EA201791630A1 - Аналитическое ультрацентрифугирование для определения характеристик рекомбинантных вирусных частиц - Google Patents

Аналитическое ультрацентрифугирование для определения характеристик рекомбинантных вирусных частиц

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Publication number
EA201791630A1
EA201791630A1 EA201791630A EA201791630A EA201791630A1 EA 201791630 A1 EA201791630 A1 EA 201791630A1 EA 201791630 A EA201791630 A EA 201791630A EA 201791630 A EA201791630 A EA 201791630A EA 201791630 A1 EA201791630 A1 EA 201791630A1
Authority
EA
Eurasian Patent Office
Prior art keywords
recombinant
particles
virus
viral particles
recombinant viral
Prior art date
Application number
EA201791630A
Other languages
English (en)
Inventor
Кэтрин Р. О'Риордан
Бренда Бернхем
Original Assignee
Джензим Корпорейшн
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55305080&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA201791630(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Джензим Корпорейшн filed Critical Джензим Корпорейшн
Publication of EA201791630A1 publication Critical patent/EA201791630A1/ru

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
    • G01N2015/045—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates by optical analysis

Abstract

В данном документе представлены способы определения характеристик препаратов на основе рекомбинантных вирусных частиц с применением аналитического ультрацентрифугирования. Рекомбинантные вирусные частицы включают частицы рекомбинантного аденоассоциированного вируса, частицы рекомбинантного аденовируса, частицы рекомбинантного лентивируса и частицы рекомбинантного вируса простого герпеса. Разные виды рекомбинантных вирусных частиц, в том числе пустые капсиды и рекомбинантные вирусные частицы с иными геномами (например, усеченными геномами, агрегатами, рекомбинантами), можно идентифицировать и количественно оценить. Способы можно применять для определения характеристик препаратов на основе рекомбинантных вирусных частиц независимо от последовательности генома рекомбинантного вируса или серотипа капсида рекомбинантного вируса.
EA201791630A 2015-01-20 2016-01-19 Аналитическое ультрацентрифугирование для определения характеристик рекомбинантных вирусных частиц EA201791630A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562105714P 2015-01-20 2015-01-20
PCT/US2016/013947 WO2016118520A1 (en) 2015-01-20 2016-01-19 Analytical ultracentrifugation for characterization of recombinant viral particles

Publications (1)

Publication Number Publication Date
EA201791630A1 true EA201791630A1 (ru) 2018-04-30

Family

ID=55305080

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201791630A EA201791630A1 (ru) 2015-01-20 2016-01-19 Аналитическое ультрацентрифугирование для определения характеристик рекомбинантных вирусных частиц

Country Status (13)

Country Link
US (5) US10429288B2 (ru)
EP (1) EP3247793A1 (ru)
JP (3) JP2018505695A (ru)
KR (1) KR20170104594A (ru)
CN (1) CN107646052A (ru)
AR (1) AR105412A1 (ru)
AU (1) AU2016209471B2 (ru)
CA (1) CA2974378A1 (ru)
EA (1) EA201791630A1 (ru)
IL (4) IL296391B1 (ru)
TW (1) TWI745285B (ru)
UY (1) UY36522A (ru)
WO (1) WO2016118520A1 (ru)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3696534A1 (en) * 2019-02-14 2020-08-19 SmartDyeLivery GmbH Method for determining physicochemical properties of nanoscale systems (nss)
TW202142692A (zh) * 2020-01-29 2021-11-16 美商航海家醫療公司 用於生產aav顆粒之方法及系統
GB202004254D0 (en) 2020-03-24 2020-05-06 Puridify Ltd Characterization of gene therapy vectors
US20220033782A1 (en) * 2020-07-29 2022-02-03 Pall Corporation Adenovirus-associated viruses separation method
JP2024511409A (ja) 2021-03-22 2024-03-13 ジェンザイム・コーポレーション 空および完全ａａｖキャプシドのサイズ排除クロマトグラフィー解析
EP4328318A1 (en) * 2021-04-19 2024-02-28 Osaka University Method for characterizing molecule delivery particles
TWI787809B (zh) * 2021-05-07 2022-12-21 台達電子工業股份有限公司 資料預篩方法及其資料預篩裝置
WO2024025787A1 (en) * 2022-07-26 2024-02-01 Brammer Bio, Llc Analytical ultracentrifugation methods
WO2024079655A1 (en) 2022-10-11 2024-04-18 Meiragtx Uk Ii Limited Chromatography methods for purification of aav capsids

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0842287B1 (en) 1995-08-03 2004-02-04 Avigen, Inc. High efficiency helper system for aav vector production
US6027931A (en) 1995-08-03 2000-02-22 Avigen, Inc. High-efficiency AA V helper functions
US6001650A (en) 1995-08-03 1999-12-14 Avigen, Inc. High-efficiency wild-type-free AAV helper functions
US5622856A (en) 1995-08-03 1997-04-22 Avigen High efficiency helper system for AAV vector production
US6593103B1 (en) * 1997-02-07 2003-07-15 The Regents Of The University Of California HIV capsid assembly-associated compositions and method
US6995006B2 (en) 1997-09-05 2006-02-07 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6566118B1 (en) 1997-09-05 2003-05-20 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
ES2478635T3 (es) 1999-08-09 2014-07-22 Targeted Genetics Corporation Incremento de la expresión de una secuencia de nucleótidos heteróloga monocatenaria de vectores virales recombinantes diseñando la secuencia de modo que forma pares de bases intracatenarios
AU7841400A (en) 1999-10-01 2001-05-10 Genovo, Incorporated Production of recombinant aav using adenovirus comprising aav rep/cap genes
ATE318923T1 (de) 2000-06-01 2006-03-15 Univ North Carolina Doppelsträngige parvovirus-vektoren
US6723551B2 (en) 2001-11-09 2004-04-20 The United States Of America As Represented By The Department Of Health And Human Services Production of adeno-associated virus in insect cells
ES2258601T3 (es) 2001-11-13 2006-09-01 The Trustees Of The University Of Pennsylvania Un metodo para la identificacion de las secuencias desconocidas del virus adeno-asociado (vaa) y un kit para el metodo.
EP2277996B1 (en) 2003-05-21 2014-09-03 Genzyme Corporation Methods for producing preparations of recombinant AAV virions substantially free of empty capsids
US7765583B2 (en) 2005-02-28 2010-07-27 France Telecom System and method for managing virtual user domains
WO2006119432A2 (en) 2005-04-29 2006-11-09 The Government Of The U.S.A., As Rep. By The Sec., Dept. Of Health & Human Services Isolation, cloning and characterization of new adeno-associated virus (aav) serotypes
EP2007795B1 (en) 2006-03-30 2016-11-16 The Board Of Trustees Of The Leland Stanford Junior University Aav capsid proteins
DK3444335T3 (da) 2009-06-16 2021-07-26 Genzyme Corp Forbedrede fremgangsmåder til oprensning af rekombinante aav-vektorer
US8663624B2 (en) 2010-10-06 2014-03-04 The Regents Of The University Of California Adeno-associated virus virions with variant capsid and methods of use thereof
EP2675902B1 (en) 2011-02-17 2019-03-27 The Trustees Of The University Of Pennsylvania Compositions and methods for altering tissue specificity and improving aav9-mediated gene transfer
FR3002237B1 (fr) 2013-02-15 2017-12-15 Genethon Methodes pour la production de particules virales aav double brin
EP3230441A4 (en) * 2014-12-12 2018-10-03 Voyager Therapeutics, Inc. Compositions and methods for the production of scaav

Also Published As

Publication number Publication date
IL274137B2 (en) 2023-02-01
US20240085301A1 (en) 2024-03-14
US20230288308A1 (en) 2023-09-14
AU2016209471A1 (en) 2017-09-07
IL274137B (en) 2022-10-01
US20200225139A1 (en) 2020-07-16
UY36522A (es) 2016-08-31
JP2018505695A (ja) 2018-03-01
WO2016118520A1 (en) 2016-07-28
IL274137A (en) 2020-06-30
CN107646052A (zh) 2018-01-30
AU2016209471B2 (en) 2022-03-31
KR20170104594A (ko) 2017-09-15
TW201639958A (zh) 2016-11-16
US11639887B2 (en) 2023-05-02
JP2021000110A (ja) 2021-01-07
IL296391A (en) 2022-11-01
CA2974378A1 (en) 2016-07-28
EP3247793A1 (en) 2017-11-29
TWI745285B (zh) 2021-11-11
AR105412A1 (es) 2017-10-04
US10429288B2 (en) 2019-10-01
IL296391B1 (en) 2024-02-01
JP2022095863A (ja) 2022-06-28
IL253542A0 (en) 2017-09-28
US20240077402A1 (en) 2024-03-07
US20180180525A1 (en) 2018-06-28
IL310375A (en) 2024-03-01
IL253542B (en) 2020-05-31

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