AR105412A1 - Ultracentrifugación analítica para la caracterización de partículas virales recombinantes - Google Patents
Ultracentrifugación analítica para la caracterización de partículas virales recombinantesInfo
- Publication number
- AR105412A1 AR105412A1 ARP160100143A ARP160100143A AR105412A1 AR 105412 A1 AR105412 A1 AR 105412A1 AR P160100143 A ARP160100143 A AR P160100143A AR P160100143 A ARP160100143 A AR P160100143A AR 105412 A1 AR105412 A1 AR 105412A1
- Authority
- AR
- Argentina
- Prior art keywords
- recombinant
- recombinant viral
- particles
- viral particles
- sedimentation
- Prior art date
Links
- 239000002245 particle Substances 0.000 title abstract 13
- 230000003612 virological effect Effects 0.000 title abstract 13
- 238000013103 analytical ultracentrifugation Methods 0.000 title abstract 3
- 238000012512 characterization method Methods 0.000 title 1
- 238000002360 preparation method Methods 0.000 abstract 4
- 238000004062 sedimentation Methods 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 210000000234 capsid Anatomy 0.000 abstract 2
- 241000700584 Simplexvirus Species 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
- G01N2015/045—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates by optical analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Se proporcionan aqui métodos para caracterizar preparaciones de partículas virales recombinantes usando ultracentrifugación analítica. Las partículas virales recombinantes incluyen partículas virales adeno-asociadas recombinantes, partículas adenovirales recombinantes, partículas lentivirales recombinantes y partículas de virus herpes simplex recombinantes. Se pueden identificar y cuantificar especies variantes de partículas virales recombinantes que incluyen cápsides vacías y partículas virales recombinantes con genomas variantes (v.g., genomas truncados, agregados, recombinantes). Los métodos se pueden usar para caracterizar preparaciones de partículas virales recombinantes independientemente de la secuencia del genoma viral recombinante o el serotipo de la cápside viral recombinante. Reivindicación 1: Un método para caracterizar una preparación de partículas virales recombinantes que comprende las etapas de a) someter la preparación a ultracentrifugación analítica en condiciones de velocidad de sedimentación del borde, en donde la sedimentación de las partículas virales recombinantes se controla a intervalos de tiempo, b) representar gráficamente el valor de la distribución de coeficientes de sedimentación diferencial (C(s)) frente al coeficiente de sedimentación en unidades Svedberg (S), y c) integrar el área bajo cada pico en la distribución C(S) para determinar la concentración relativa de cada pico, en donde cada pico representa una especie de partícula viral recombinante viral recombinante.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562105714P | 2015-01-20 | 2015-01-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR105412A1 true AR105412A1 (es) | 2017-10-04 |
Family
ID=55305080
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP160100143A AR105412A1 (es) | 2015-01-20 | 2016-01-20 | Ultracentrifugación analítica para la caracterización de partículas virales recombinantes |
Country Status (13)
Country | Link |
---|---|
US (4) | US10429288B2 (es) |
EP (1) | EP3247793A1 (es) |
JP (3) | JP2018505695A (es) |
KR (1) | KR20170104594A (es) |
CN (1) | CN107646052A (es) |
AR (1) | AR105412A1 (es) |
AU (1) | AU2016209471B2 (es) |
CA (1) | CA2974378A1 (es) |
EA (1) | EA201791630A1 (es) |
IL (4) | IL310375A (es) |
TW (1) | TWI745285B (es) |
UY (1) | UY36522A (es) |
WO (1) | WO2016118520A1 (es) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EA201791630A1 (ru) | 2015-01-20 | 2018-04-30 | Джензим Корпорейшн | Аналитическое ультрацентрифугирование для определения характеристик рекомбинантных вирусных частиц |
EP3696534A1 (en) * | 2019-02-14 | 2020-08-19 | SmartDyeLivery GmbH | Method for determining physicochemical properties of nanoscale systems (nss) |
TW202142692A (zh) * | 2020-01-29 | 2021-11-16 | 美商航海家醫療公司 | 用於生產aav顆粒之方法及系統 |
GB202004254D0 (en) | 2020-03-24 | 2020-05-06 | Puridify Ltd | Characterization of gene therapy vectors |
US20220033782A1 (en) * | 2020-07-29 | 2022-02-03 | Pall Corporation | Adenovirus-associated viruses separation method |
JP2024511409A (ja) | 2021-03-22 | 2024-03-13 | ジェンザイム・コーポレーション | 空および完全aavキャプシドのサイズ排除クロマトグラフィー解析 |
JP7454883B2 (ja) * | 2021-04-19 | 2024-03-25 | 国立大学法人大阪大学 | 分子送達用粒子を特徴づけるための方法 |
TWI787809B (zh) * | 2021-05-07 | 2022-12-21 | 台達電子工業股份有限公司 | 資料預篩方法及其資料預篩裝置 |
WO2024025787A1 (en) * | 2022-07-26 | 2024-02-01 | Brammer Bio, Llc | Analytical ultracentrifugation methods |
WO2024079655A1 (en) | 2022-10-11 | 2024-04-18 | Meiragtx Uk Ii Limited | Chromatography methods for purification of aav capsids |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6001650A (en) * | 1995-08-03 | 1999-12-14 | Avigen, Inc. | High-efficiency wild-type-free AAV helper functions |
CA2228269C (en) | 1995-08-03 | 2008-01-08 | Avigen, Inc. | High efficiency helper system for aav vector production |
US5622856A (en) | 1995-08-03 | 1997-04-22 | Avigen | High efficiency helper system for AAV vector production |
US6027931A (en) | 1995-08-03 | 2000-02-22 | Avigen, Inc. | High-efficiency AA V helper functions |
WO1998035062A1 (en) * | 1997-02-07 | 1998-08-13 | Lingappa Jaisri R | Multistep, atp-dependent cell-free system for the assembly of human immunodeficiency virus capsids |
US6989264B2 (en) | 1997-09-05 | 2006-01-24 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
US6566118B1 (en) | 1997-09-05 | 2003-05-20 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
JP4827353B2 (ja) | 1999-08-09 | 2011-11-30 | ターゲティッド ジェネティクス コーポレイション | 鎖内塩基対を形成するような配列の設計による、組換えウイルスベクターからの一本鎖の異種ヌクレオチド配列の発現の増大 |
JP2003511037A (ja) * | 1999-10-01 | 2003-03-25 | ジェノボ, インコーポレイテッド | AAVrep/cap遺伝子を含むアデノウイルスを使用する組換えAAVの産生 |
DE60117550T2 (de) | 2000-06-01 | 2006-12-07 | University Of North Carolina At Chapel Hill | Doppelsträngige parvovirus-vektoren |
US6723551B2 (en) | 2001-11-09 | 2004-04-20 | The United States Of America As Represented By The Department Of Health And Human Services | Production of adeno-associated virus in insect cells |
SG157224A1 (en) | 2001-11-13 | 2009-12-29 | Univ Pennsylvania | A method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby |
EP2281877A3 (en) | 2003-05-21 | 2011-06-01 | Genzyme Corporation | Methods for producing preparations of recombinant AAV virions substantially free of empty capsids |
US7765583B2 (en) | 2005-02-28 | 2010-07-27 | France Telecom | System and method for managing virtual user domains |
WO2006119432A2 (en) | 2005-04-29 | 2006-11-09 | The Government Of The U.S.A., As Rep. By The Sec., Dept. Of Health & Human Services | Isolation, cloning and characterization of new adeno-associated virus (aav) serotypes |
EP2007795B1 (en) | 2006-03-30 | 2016-11-16 | The Board Of Trustees Of The Leland Stanford Junior University | Aav capsid proteins |
SI3067417T1 (sl) | 2009-06-16 | 2018-11-30 | Genzyme Corporation | Izboljšani postopki čiščenja vektorjev rekombinantnega AAV |
US8663624B2 (en) | 2010-10-06 | 2014-03-04 | The Regents Of The University Of California | Adeno-associated virus virions with variant capsid and methods of use thereof |
SG10201601110VA (en) | 2011-02-17 | 2016-03-30 | Univ Pennsylvania | Compositions and methods for altering tissue specificity and improving aav9-mediated gene transfer |
FR3002237B1 (fr) | 2013-02-15 | 2017-12-15 | Genethon | Methodes pour la production de particules virales aav double brin |
EP3230441A4 (en) * | 2014-12-12 | 2018-10-03 | Voyager Therapeutics, Inc. | Compositions and methods for the production of scaav |
EA201791630A1 (ru) | 2015-01-20 | 2018-04-30 | Джензим Корпорейшн | Аналитическое ультрацентрифугирование для определения характеристик рекомбинантных вирусных частиц |
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2016
- 2016-01-19 EA EA201791630A patent/EA201791630A1/ru unknown
- 2016-01-19 AU AU2016209471A patent/AU2016209471B2/en active Active
- 2016-01-19 IL IL310375A patent/IL310375A/en unknown
- 2016-01-19 US US15/544,498 patent/US10429288B2/en active Active
- 2016-01-19 WO PCT/US2016/013947 patent/WO2016118520A1/en active Application Filing
- 2016-01-19 CA CA2974378A patent/CA2974378A1/en active Pending
- 2016-01-19 KR KR1020177023001A patent/KR20170104594A/ko not_active Application Discontinuation
- 2016-01-19 JP JP2017556788A patent/JP2018505695A/ja active Pending
- 2016-01-19 EP EP16703205.1A patent/EP3247793A1/en active Pending
- 2016-01-19 IL IL296391A patent/IL296391B2/en unknown
- 2016-01-19 TW TW105101529A patent/TWI745285B/zh active
- 2016-01-19 CN CN201680016288.9A patent/CN107646052A/zh active Pending
- 2016-01-20 AR ARP160100143A patent/AR105412A1/es unknown
- 2016-01-20 UY UY0001036522A patent/UY36522A/es not_active Application Discontinuation
-
2017
- 2017-07-18 IL IL253542A patent/IL253542B/en active IP Right Grant
-
2019
- 2019-08-21 US US16/547,144 patent/US11639887B2/en active Active
-
2020
- 2020-04-22 IL IL274137A patent/IL274137B2/en unknown
- 2020-09-11 JP JP2020152446A patent/JP2021000110A/ja active Pending
-
2022
- 2022-04-12 JP JP2022065460A patent/JP2022095863A/ja active Pending
-
2023
- 2023-03-22 US US18/188,176 patent/US20230288308A1/en active Pending
- 2023-11-10 US US18/506,853 patent/US12013326B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EA201791630A1 (ru) | 2018-04-30 |
IL296391B2 (en) | 2024-06-01 |
TWI745285B (zh) | 2021-11-11 |
EP3247793A1 (en) | 2017-11-29 |
IL253542B (en) | 2020-05-31 |
IL274137B2 (en) | 2023-02-01 |
IL274137B (en) | 2022-10-01 |
WO2016118520A1 (en) | 2016-07-28 |
UY36522A (es) | 2016-08-31 |
US20230288308A1 (en) | 2023-09-14 |
US20180180525A1 (en) | 2018-06-28 |
US10429288B2 (en) | 2019-10-01 |
IL310375A (en) | 2024-03-01 |
IL296391A (en) | 2022-11-01 |
JP2018505695A (ja) | 2018-03-01 |
US20240085301A1 (en) | 2024-03-14 |
JP2022095863A (ja) | 2022-06-28 |
CN107646052A (zh) | 2018-01-30 |
IL253542A0 (en) | 2017-09-28 |
US20240077402A1 (en) | 2024-03-07 |
AU2016209471B2 (en) | 2022-03-31 |
US12013326B2 (en) | 2024-06-18 |
AU2016209471A1 (en) | 2017-09-07 |
TW201639958A (zh) | 2016-11-16 |
US20200225139A1 (en) | 2020-07-16 |
JP2021000110A (ja) | 2021-01-07 |
CA2974378A1 (en) | 2016-07-28 |
IL274137A (en) | 2020-06-30 |
KR20170104594A (ko) | 2017-09-15 |
IL296391B1 (en) | 2024-02-01 |
US11639887B2 (en) | 2023-05-02 |
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