UY25092A1 - Sistema de transformacion en base a polen utilizando medios solidos - Google Patents

Sistema de transformacion en base a polen utilizando medios solidos

Info

Publication number
UY25092A1
UY25092A1 UY25092A UY25092A UY25092A1 UY 25092 A1 UY25092 A1 UY 25092A1 UY 25092 A UY25092 A UY 25092A UY 25092 A UY25092 A UY 25092A UY 25092 A1 UY25092 A1 UY 25092A1
Authority
UY
Uruguay
Prior art keywords
pollen
plant
plants
transgenic
agrobacteria
Prior art date
Application number
UY25092A
Other languages
English (en)
Inventor
j burke John
j oliver Melvin
P Velter Jeffrey
Original Assignee
Us Secretaty Of Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Secretaty Of Agriculture filed Critical Us Secretaty Of Agriculture
Publication of UY25092A1 publication Critical patent/UY25092A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se relaciona con el campo general de la ingeniería genética vegetal, en particular con la transformación de material genético exógeno en la línea germinal de líneas vegetales mediante una transformación de Agrobacteria en base a polen. Un método para producir una planta transgénica que comprende: a) obtener polen de una primera planta, b) aplicar un césped de Agrobacterias a un medio sólido de cultivo de polen, comprendiendo las Agrobacterias por lo menos una secuencia génica heteróloga capaz de ser transferida a una célula vegetal, c) aplicar el polen al medio sólido, d) dejar que el polen germine y crezca en el medio, produciendo de esta manera polen transgénico, e) aplicar el polen transgéncio al estigma de la segunda planta capaz de ser fertilizada por el polen de la primera planta, fertilizando de esta manera la segunda planta, f) obtener semillas transgénicas de la segunda planta, g) germinar la semilla transgénica para obtener una planta transgénica. La primera y segunda planta se seleccionan del grupo que consiste en plantas de tabaco, plantas de algodón y plantas de soja. Se caracteriza porque las plantas son plantas con polen de estigma seco. El medio sólido de cultivo de polen comprende agarosa al 10%, sacarosa al 25%, 0.52 mM de KNO3, 3.06 mM de MnSO4, 1.66 mM de H3BO3, 0.42 mM de MgSO4.7H2O y 1.0 microM de ácido giberélico A3.
UY25092A 1997-07-15 1998-07-14 Sistema de transformacion en base a polen utilizando medios solidos UY25092A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/892,735 US5929300A (en) 1997-07-15 1997-07-15 Pollen-based transformation system using solid media

Publications (1)

Publication Number Publication Date
UY25092A1 true UY25092A1 (es) 1999-01-04

Family

ID=25400416

Family Applications (1)

Application Number Title Priority Date Filing Date
UY25092A UY25092A1 (es) 1997-07-15 1998-07-14 Sistema de transformacion en base a polen utilizando medios solidos

Country Status (20)

Country Link
US (1) US5929300A (es)
EP (1) EP0996328B1 (es)
CN (1) CN1098029C (es)
AR (1) AR016332A1 (es)
AT (1) ATE233476T1 (es)
AU (1) AU733080B2 (es)
BR (1) BR9811791A (es)
CA (1) CA2296501C (es)
CO (1) CO4810213A1 (es)
DE (1) DE69811897T2 (es)
DK (1) DK0996328T3 (es)
EG (1) EG21999A (es)
ES (1) ES2193545T3 (es)
HK (1) HK1026118A1 (es)
PE (1) PE20000184A1 (es)
PT (1) PT996328E (es)
TW (1) TW577922B (es)
UY (1) UY25092A1 (es)
WO (1) WO1999003326A1 (es)
ZA (1) ZA986240B (es)

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US6806399B1 (en) * 2000-04-19 2004-10-19 Carmel-Haifa University Economic Corporation Ltd. Pollen-mediated method for transformation of maize, tomato or melon
KR100451666B1 (ko) * 2001-11-19 2004-10-08 박희성 식물화분을 이용한 재조합 단백질의 생산방법
AU2006237317B8 (en) 2005-04-19 2011-05-12 Basf Plant Science Gmbh Improved methods controlling gene expression
WO2006122023A1 (en) 2005-05-09 2006-11-16 The Samuel Roberts Noble Foundation, Inc. Agrobacterium transformation of stolons
CN101268194A (zh) 2005-09-20 2008-09-17 巴斯福植物科学有限公司 使用ta-siRNA调控基因表达的方法
ES2302627B2 (es) * 2006-10-03 2009-07-09 Universidad Politecnica De Valencia Metodo de evaluacion de la eficacia de genes de tolerancia a estres abiotico en plantas transgenicas.
CN101605894A (zh) 2007-02-08 2009-12-16 巴斯福植物科学有限公司 用opr3-样基因的rna干扰控制线虫的组合物和方法
CN104004702A (zh) * 2014-05-20 2014-08-27 四川农业大学 用于四倍体扁穗牛鞭草花粉萌发的培养基
CN104726391B (zh) * 2015-04-21 2018-04-13 河北双星种业股份有限公司 一种向日葵花粉离体萌发的培养基及测定向日葵花粉活力的方法
CN107475283B (zh) * 2016-07-12 2023-10-13 山西省农业科学院玉米研究所 一种超声波辅助农杆菌介导花粉的玉米转基因方法
CN109652455B (zh) * 2019-02-19 2022-09-23 南京农业大学 一种磁性纳米载体介导的不结球白菜高效遗传转化方法及其应用
CN110938583A (zh) * 2019-12-25 2020-03-31 石河子大学 一种棉花花粉萌发的固体培养基和萌发方法
CN117178964B (zh) * 2023-11-06 2024-01-12 海南大学三亚南繁研究院 一种适用于粉蚧人工接种植物病毒的装置

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SU538698A1 (ru) * 1975-07-03 1976-12-15 Государственный Ордена Трудового Красного Знамени Никитский Ботанический Сад Способ проращивани пыльцы тисовых, таксодиевых и кипарисовых
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
US5034322A (en) 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
EP0160692A1 (en) * 1983-11-03 1985-11-13 DE WET, Johannes Martenis Jacob Method for the transfer of exogenous genes in plants using pollen as a vector
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4743548A (en) * 1984-09-25 1988-05-10 Calgene, Inc. Plant cell microinjection technique
US5100792A (en) * 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
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US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
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CA1327173C (en) * 1987-07-21 1994-02-22 Erwin Heberle-Bors Method of gene transfer into plants
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US5629183A (en) * 1989-05-08 1997-05-13 The United States Of America As Represented By The Secretary Of Agriculture Plant transformation by gene transfer into pollen
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JPH08182494A (ja) * 1994-11-04 1996-07-16 Showa Denko Kk 固体培地

Also Published As

Publication number Publication date
TW577922B (en) 2004-03-01
ZA986240B (en) 1999-02-05
ES2193545T3 (es) 2003-11-01
CA2296501A1 (en) 1999-01-28
AU8400598A (en) 1999-02-10
DE69811897D1 (de) 2003-04-10
AR016332A1 (es) 2001-07-04
PE20000184A1 (es) 2000-03-07
ATE233476T1 (de) 2003-03-15
EP0996328A1 (en) 2000-05-03
EP0996328B1 (en) 2003-03-05
PT996328E (pt) 2003-07-31
CN1263434A (zh) 2000-08-16
CN1098029C (zh) 2003-01-08
CA2296501C (en) 2008-10-21
AU733080B2 (en) 2001-05-03
CO4810213A1 (es) 1999-06-30
WO1999003326A1 (en) 1999-01-28
DE69811897T2 (de) 2003-12-18
US5929300A (en) 1999-07-27
EG21999A (en) 2002-05-31
BR9811791A (pt) 2000-09-26
HK1026118A1 (en) 2000-12-08
DK0996328T3 (da) 2003-06-30

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Effective date: 20180714