PE20000184A1 - Sistema de transformacion a base de polen usando medios solidos - Google Patents

Sistema de transformacion a base de polen usando medios solidos

Info

Publication number
PE20000184A1
PE20000184A1 PE1998000616A PE00061698A PE20000184A1 PE 20000184 A1 PE20000184 A1 PE 20000184A1 PE 1998000616 A PE1998000616 A PE 1998000616A PE 00061698 A PE00061698 A PE 00061698A PE 20000184 A1 PE20000184 A1 PE 20000184A1
Authority
PE
Peru
Prior art keywords
pollen
plant
transgenic
plants
agrobacteria
Prior art date
Application number
PE1998000616A
Other languages
English (en)
Inventor
John J Burke
Melvin J Oliver
Jeffrey P Velten
Original Assignee
Us Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Agriculture filed Critical Us Agriculture
Publication of PE20000184A1 publication Critical patent/PE20000184A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

SE REFIERE A UN METODO PARA PRODUCIR UNA PLANTA TRANSGENICA QUE COMPRENDE a)OBTENER POLEN DE UNA PRIMERA PLANTA; b)APLICAR UNA CAPA DE AGROBACTERIA TRANSGENICA EN UN MEDIO DE GERMINACION DE POLEN, QUE COMPRENDE 10% DE AGAROSA, 25% DE SACAROSA, 0,52 mM DE KNO3, 3,06 mM DE MnSO4, 1,66 mM DE H3BO3, 0,42 mM DE MgSO4.7H20 Y 1,0 µm A3 DE ACIDO GIBERELICO; DONDE LA AGROBACTERIA COMPRENDE UNA SECUENCIA DE GENES HETEROLOGOS CAPAZ DE SER TRANSFERIDA A UNA CELULA VEGETAL; c)APLICAR POLEN AL MEDIO DE GERMINACION; d)DEJAR QUE EL POLEN GERMINE Y CREZCA EN EL MEDIO, PRODUCIENDO ASI POLEN TRANSGENICO; e)APLICAR EL POLEN TRANSGENICO AL ESTIGMA DE UNA SEGUNDA PLANTA CAPAZ DE SER FERTILIZADA POR EL POLEN DE LA PRIMERA PLANTA; f)OBTENER SEMILLAS TRANSGENICAS DE LA SEGUNDA PLANTA; g)HACER GERMINAR LA SEMILLA TRANSGENICA A FIN DE OBTENER UNA PLANTA TRANSGENICA. LA PRIMERA Y SEGUNDA PLANTA SON SELECCIONADAS DE PLANTAS DE TABACO, PLANTAS DE ALGODON O ALGODON EMASCULADA Y PLANTAS DE SOJA. EL POLEN DE LAS PLANTAS SON DE ESTIGMA SECO
PE1998000616A 1997-07-15 1998-07-10 Sistema de transformacion a base de polen usando medios solidos PE20000184A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/892,735 US5929300A (en) 1997-07-15 1997-07-15 Pollen-based transformation system using solid media

Publications (1)

Publication Number Publication Date
PE20000184A1 true PE20000184A1 (es) 2000-03-07

Family

ID=25400416

Family Applications (1)

Application Number Title Priority Date Filing Date
PE1998000616A PE20000184A1 (es) 1997-07-15 1998-07-10 Sistema de transformacion a base de polen usando medios solidos

Country Status (20)

Country Link
US (1) US5929300A (es)
EP (1) EP0996328B1 (es)
CN (1) CN1098029C (es)
AR (1) AR016332A1 (es)
AT (1) ATE233476T1 (es)
AU (1) AU733080B2 (es)
BR (1) BR9811791A (es)
CA (1) CA2296501C (es)
CO (1) CO4810213A1 (es)
DE (1) DE69811897T2 (es)
DK (1) DK0996328T3 (es)
EG (1) EG21999A (es)
ES (1) ES2193545T3 (es)
HK (1) HK1026118A1 (es)
PE (1) PE20000184A1 (es)
PT (1) PT996328E (es)
TW (1) TW577922B (es)
UY (1) UY25092A1 (es)
WO (1) WO1999003326A1 (es)
ZA (1) ZA986240B (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6806399B1 (en) * 2000-04-19 2004-10-19 Carmel-Haifa University Economic Corporation Ltd. Pollen-mediated method for transformation of maize, tomato or melon
KR100451666B1 (ko) * 2001-11-19 2004-10-08 박희성 식물화분을 이용한 재조합 단백질의 생산방법
US9085774B2 (en) 2005-04-19 2015-07-21 Basf Plant Science Gmbh Methods controlling gene expression
US7611898B2 (en) 2005-05-09 2009-11-03 The Samuel Roberts Noble Foundation Agrobacterium transformation of stolons
EP2980220A1 (en) 2005-09-20 2016-02-03 BASF Plant Science GmbH Improved methods controlling gene expression
ES2302627B2 (es) * 2006-10-03 2009-07-09 Universidad Politecnica De Valencia Metodo de evaluacion de la eficacia de genes de tolerancia a estres abiotico en plantas transgenicas.
EP2111452B1 (en) 2007-02-08 2012-04-11 BASF Plant Science GmbH Compositions and methods using rna interference of opr3-like gene for control of nematodes
CN104004702A (zh) * 2014-05-20 2014-08-27 四川农业大学 用于四倍体扁穗牛鞭草花粉萌发的培养基
CN104726391B (zh) * 2015-04-21 2018-04-13 河北双星种业股份有限公司 一种向日葵花粉离体萌发的培养基及测定向日葵花粉活力的方法
CN107475283B (zh) * 2016-07-12 2023-10-13 山西省农业科学院玉米研究所 一种超声波辅助农杆菌介导花粉的玉米转基因方法
CN109652455B (zh) * 2019-02-19 2022-09-23 南京农业大学 一种磁性纳米载体介导的不结球白菜高效遗传转化方法及其应用
CN110938583A (zh) * 2019-12-25 2020-03-31 石河子大学 一种棉花花粉萌发的固体培养基和萌发方法
CN117178964B (zh) * 2023-11-06 2024-01-12 海南大学三亚南繁研究院 一种适用于粉蚧人工接种植物病毒的装置

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SU538698A1 (ru) * 1975-07-03 1976-12-15 Государственный Ордена Трудового Красного Знамени Никитский Ботанический Сад Способ проращивани пыльцы тисовых, таксодиевых и кипарисовых
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
US5034322A (en) 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
WO1985001856A1 (en) * 1983-11-03 1985-05-09 Johannes Martenis Jacob De Wet Method for the transfer of exogenous genes in plants using pollen as a vector
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4743548A (en) * 1984-09-25 1988-05-10 Calgene, Inc. Plant cell microinjection technique
US5100792A (en) * 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US5569597A (en) * 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
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US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
US5187073A (en) * 1986-06-30 1993-02-16 The University Of Toledo Process for transforming gramineae and the products thereof
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CA1327173C (en) * 1987-07-21 1994-02-22 Erwin Heberle-Bors Method of gene transfer into plants
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JPH08182494A (ja) * 1994-11-04 1996-07-16 Showa Denko Kk 固体培地

Also Published As

Publication number Publication date
CA2296501C (en) 2008-10-21
EG21999A (en) 2002-05-31
PT996328E (pt) 2003-07-31
WO1999003326A1 (en) 1999-01-28
CA2296501A1 (en) 1999-01-28
US5929300A (en) 1999-07-27
AR016332A1 (es) 2001-07-04
AU733080B2 (en) 2001-05-03
CN1263434A (zh) 2000-08-16
EP0996328A1 (en) 2000-05-03
BR9811791A (pt) 2000-09-26
HK1026118A1 (en) 2000-12-08
TW577922B (en) 2004-03-01
ATE233476T1 (de) 2003-03-15
DE69811897D1 (de) 2003-04-10
DE69811897T2 (de) 2003-12-18
EP0996328B1 (en) 2003-03-05
ES2193545T3 (es) 2003-11-01
AU8400598A (en) 1999-02-10
CN1098029C (zh) 2003-01-08
ZA986240B (en) 1999-02-05
UY25092A1 (es) 1999-01-04
CO4810213A1 (es) 1999-06-30
DK0996328T3 (da) 2003-06-30

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