US4286088A - Process for preparing 7-aminocephalosporins - Google Patents
Process for preparing 7-aminocephalosporins Download PDFInfo
- Publication number
- US4286088A US4286088A US05/966,654 US96665478A US4286088A US 4286088 A US4286088 A US 4286088A US 96665478 A US96665478 A US 96665478A US 4286088 A US4286088 A US 4286088A
- Authority
- US
- United States
- Prior art keywords
- group
- halide
- process according
- tetrazol
- imino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D501/00—Heterocyclic compounds containing 5-thia-1-azabicyclo [4.2.0] octane ring systems, i.e. compounds containing a ring system of the formula:, e.g. cephalosporins; Such ring systems being further condensed, e.g. 2,3-condensed with an oxygen-, nitrogen- or sulfur-containing hetero ring
- C07D501/14—Compounds having a nitrogen atom directly attached in position 7
- C07D501/16—Compounds having a nitrogen atom directly attached in position 7 with a double bond between positions 2 and 3
- C07D501/18—7-Aminocephalosporanic or substituted 7-aminocephalosporanic acids
Definitions
- This invention relates to a process for preparing 7-aminocephalosporins, which comprises cleaving the amide group at 7-position of a compound represented by the following formula or its salt: ##STR3## wherein R 1 stands for an amino group which may be protected, and R 2 stands for 3-oxobutyryloxy group, 1-methyl-1H-tetrazol-5-ylthio group or 1-(2-dimethylaminoethyl)-1H-tetrazol-5-ylthio group, to produce a compound represented by the following formula or its salt: ##STR4## wherein R 2 is the same as defined above.
- the compound (II) can be prepared from the compound (I) with good yield when the carboxyl group at 4-position of the compound (I) is protected by acetyl or propionyl halide such as acetyl chloride or propionyl chloride.
- the present invention is accomplished based on such a finding.
- the starting compound (I) to be used in this reaction can be obtained according to the method as described in German Laid-open Specification No. 2607064 (corresponding to Belgian Pat. No. 838833) or methods similar thereto.
- the compound (I) may be used in the free form or in the salt form with an alkali or alkaline earth metal such as sodium, potassium, calcium, lithium and the like; a basic amino acid such as lysine, arginine, ornithine, histidine and the like; organic amine such as pyridine, picoline, N-methylmorpholine, quinoline, isoquinoline, N,N-dimethylaniline, triethylamine and the like; a polyhydroxy alkylamine such as N-methyl gulcamine, di-ethanolamine, tri-ethanolamine, tris-hydroxy methylaminomethane and the like; or an acid such as hydrochloric acid, sulfuric acid, trifluoroacetic acid and the like.
- R 1 represents an amino group which may be protected before protecting the carboxyl group at 4-position.
- protective groups there may be used those conventionally used in peptide chemistry and all protective groups for amino groups at 6- or 7-acyl groups of penicillins or cephalosporins are applicable here. Since it is not required to eliminate the protective group in the process of the present invention, there may also be employed groups of which elimination is difficult or impossible, for example, acrylamino groups.
- the acyl groups in said acylamino groups frequently used may include phthaloyl, benzoyl, p-nitrobenzoyl, toluoyl, naphthoyl, p-tert-butylbenzoyl and so on.
- the starting compound (I) thus prepared may also be provided for use as it is, without specific isolation or purification, in the reaction for preparation of the compound (II).
- the compound (II) can be prepared from the compound (I) according to the following procedures.
- the compound (I) is allowed to react with an acetyl or propionyl halide such as acetyl chloride or propionyl chloride to protect the carboxyl group at 4-position.
- This reaction may advantageously be carried out in an inert solvent such as dichloromethane, chloroform, 1,2-dichloroethane or tetrahydrofuran, under anhydrous conditions, in the presence of a tertiary amine such as trimethyl amine, triethyl amine, quinoline, pyridine, N,N-dimethyl aniline, N,N-diethyl aniline, N-methyl morpholine.
- an inert solvent such as dichloromethane, chloroform, 1,2-dichloroethane or tetrahydrofuran
- a tertiary amine such as trimethyl amine, triethyl amine
- the compound (I) is dissolved in the solvent after formation of salt with these amines.
- This protection reaction may be conducted at -50° C. or higher, but side reactions will occur at around room temperature to give no product with high purity. Thus, it is preferred to conduct the reaction at -50° C. to 0° C., particularly at -10° C. to -50° C. Since the carboxyl group in the acylamino group at 7-position is protected by acetyl halide as well as one at 4-position, the protective agent is added in an amount of at least twice, preferably 8-12 times as much as moles of the compound to be protected in order to obtain favorable results.
- reaction product as prepared above is allowed to react with an imino halide-forming agent, e.g. phosphorous pentachloride, etc., to be converted to an imino halide.
- an imino halide-forming agent e.g. phosphorous pentachloride, etc.
- This reaction may preferably be carried out in an inert solvent as mentioned above in the presence of a tertiary amine such as N,N-dimethyl aniline, N,N-diethyl aniline, etc.
- the reaction temperature is not specifically limited but preferably in the range from -55° C. to 0° C.
- the thus prepared imino ether is subjected to solvolysis with water, lower alkanols as mentioned above or others.
- water is added to the reaction mixture to effect hydrolysis, followed by isolation purification according to conventional method such as precipitation of the objective compound by adjusting pH of the reaction mixture to around isoelectric point of the product (II) or removal of the solvent.
- the free compound (II) and its salt as set forth in connection with the compound (I) are important intermediates for preparation of antimicrobial substances.
- U.S. Pat. No. 4,080,498 corresponding to British Pat. No. 1491081, Belgian Pat. No. 823861 and South African Pat. No. 7418050
- Japanese patent application No. 37374/1976 corresponding to German Laid-open Specification 2714419
- 3-(4-carbamoyl-1-pyridiniomethyl)-7 ⁇ -(D- ⁇ -sulfophenylacetamido)-ceph-3-em-4-carboxylate monosodium salt obtained by reacting 7-AOC and D- ⁇ -sulfophenyl acetyl chloride, and then with iso-nicotinic acid amide, as disclosed by Japanese patent application No. 83869/1977 (corresponding to German Laid-open Specification No. 2607064), is a compound having strong antimicrobial activity against Pseudomonas.
- N,N-dimethyl aniline (19 ml.) is added to the resultant reaction mixture and the mixture is cooled to -50° C., followed by stirring for 20 minutes with addition of phosphorous pentachloride (12.0 g).
- Methanol 50 ml.
- Methanol is added to the reaction mixture at not higher than -30° C., followed by stirring at -20° C. to -15° C. for 20 minutes, and the mixture is further vigorously agitated with addition of water (100 ml.) for 5 minutes. After the mixture is left to stand, the aqueous layer is separated. The aqueous solution is mixed with methanol (55 ml.) and adjusted under stirring with a saturated aqueous potassium carbonate solution at room temperature to pH 3.4.
- the reaction mixture is subsequently mixed with phosphorous pentachloride (6.0 g) at -50° C., followed by stirring at -45° C. to -40° C. for 25 minutes, and methanol (25 ml.) is added dropwise slowly thereto. During addition of the methanol, the inner temperature is maintained at not higher than -30° C. Then, water (50 ml.) is added to the reaction mixture and, after stirring the mixture vigorously at -5° C. to 0° C. for 5 minutes, the aqueous layer is separated. The organic layer is further subjected to extraction with water (5 ml.).
- the aqueous layer is separated, washed with dichloromethane (100 ml.), mixed with ethyl acetate (100 ml.) under stirring and adjusted to pH 6.0 with triethyl amine.
- the aqueous layer is separated, adjusted to pH 3.2 with 4 N-HCl, then concentrated under reduced pressure and poured into ethanol (1 liter). After stirring the precipitates at not higher than 5° C.
- Example 4 According to the same procedures as described in Example 4 except that propionyl chloride (51 g) is used in place of acetyl chloride, 7-AMTC-mono-hydrochloride is prepared.
- the IR and NMR spectra of this product are substantially identical with those of the product obtained in Example 4. Yield: 16.6 g, *2 Purity: 89.0%, Yield percentage: 86%.
- reaction mixture is cooled to -55° C. and phosphorous pentachloride (34 g) is added thereto.
- phosphorous pentachloride 34 g
- the reaction is carried out at -55° C. to -50° C. for 45 minutes.
- iso-butanol (170 ml.) is added dropwise to the reaction mixture, cooling is discontinued and the reaction mixture is warmed to room temperature. Stirring is continued at room temperature for 20 minutes and then at 0° C. for 30 minutes.
- Example 7 In place of adding acetyl chloride and stirring the mixture at -20° C. to -15° C. for 10 minutes in Example 7, propionyl chloride (56 g) is added and the mixture is stirred at -20° C. to -15° C. for 10 minutes, following otherwise the same procedures as in Example 7, 7-AMTC mono-hydrochloride is obtained. Yield: 15.6 g; *2 Purity: 80%; Yield percentage: 84%. The IR(KBr) spectrum and NMR(D 2 O) spectrum of this product are substantially identical with absorption spectra of those of Example 4.
- reaction mixture is cooled to -30° C., at which acetyl chloride is added dropwise thereto, and the mixture is then stirred at -25° C. to -20° C. for 20 minutes.
- N,N-dimethyl aniline (33 g) and then phosphorous pentachloride (21 g) are added thereto, followed by stirring at -55° C. to -50° C. for 40 minutes.
- iso-butanol 85 ml. is added dropwise at not higher than -30° C. to the mixture and the reaction is carried out at -35° C. to -30° C. for one hour.
- the concentrate is then poured into ethanol (500 ml.) and, after stirring the mixture at 0° C. to 5° C. for 30 minutes, the precipitates are collected by filtration and washed with ethanol and then with acetone, followed by drying under reduced pressure, to give 7 ⁇ -amino-3-[[[1-(2-dimethylaminoehyl)-1H-tetrazol-5-yl]thio]methyl]ceph-3-em-4-carboxylic acid mono-hydrochloride.
- the IR and NMR spectra of this product are substantially identical with those of the product obtained in Example 4. Yield: 8.2 g, *2 Purity: 81.0%, Yield percentage: 84%.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cephalosporin Compounds (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP52149718A JPS5851956B2 (ja) | 1977-12-12 | 1977-12-12 | セフアロスポリン化合物の製造法 |
JP52/149718 | 1977-12-12 | ||
JP52151105A JPS5919552B2 (ja) | 1977-12-14 | 1977-12-14 | セフアロスポリン化合物の製造法 |
JP52/151105 | 1977-12-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
US4286088A true US4286088A (en) | 1981-08-25 |
Family
ID=26479512
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US05/966,654 Expired - Lifetime US4286088A (en) | 1977-12-12 | 1978-12-05 | Process for preparing 7-aminocephalosporins |
Country Status (8)
Country | Link |
---|---|
US (1) | US4286088A (da) |
CH (1) | CH638219A5 (da) |
DE (1) | DE2853176A1 (da) |
ES (1) | ES475880A1 (da) |
FR (1) | FR2411198A1 (da) |
GB (1) | GB2009736B (da) |
GR (1) | GR70261B (da) |
IT (1) | IT1101736B (da) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1239814A (en) * | 1967-08-07 | 1971-07-21 | Koninklijke Gist Spiritus | Process for the preparation of 7-aminocephalosporanic acid and its derivatives |
US3839328A (en) * | 1969-11-13 | 1974-10-01 | Lilly Co Eli | Use of mixed anhydride protecting group in cleaving acyl groups from cephalosporins and penicillins |
US3931161A (en) * | 1972-03-09 | 1976-01-06 | Alfa Farmaceutici S.P.A. | Cephalosporin derivatives |
US3932392A (en) * | 1974-01-14 | 1976-01-13 | Bristol-Myers Company | Process for the preparation of 7-aminocephalosporanic acids |
US4036833A (en) * | 1974-05-28 | 1977-07-19 | Toshiyasu Ishimaru | 7-[(5'-N-methylthioacetamido)-adipoamido] cephalosporin derivatives |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE758800A (fr) * | 1969-11-13 | 1971-05-12 | Lilly Co Eli | Methode de coupure du groupement 7-carboxamide d'un compose de cephalosporine |
GB1391271A (en) * | 1971-04-30 | 1975-04-16 | Glaxo Lab Ltd | Preparation of antibiotic compounds |
BE788142A (fr) * | 1971-09-03 | 1973-02-28 | Lilly Co Eli | Procede de preparation de l'acide 7-aminocephalosporanique |
JPS50149694A (da) | 1974-05-21 | 1975-11-29 | ||
JPS5826355B2 (ja) * | 1974-07-24 | 1983-06-02 | ザイダンホウジン サンギヨウカガクケンキユウキヨウカイ | 7− アミノセフアロスポランサンユウドウタイノ セイゾウホウホウ |
DK159154C (da) * | 1975-02-24 | 1991-02-11 | Takeda Chemical Industries Ltd | Fremgangsmaade til fremstilling af cephalosporinderivater eller salte deraf |
-
1978
- 1978-11-24 GR GR57733A patent/GR70261B/el unknown
- 1978-12-05 US US05/966,654 patent/US4286088A/en not_active Expired - Lifetime
- 1978-12-06 GB GB7847436A patent/GB2009736B/en not_active Expired
- 1978-12-08 DE DE19782853176 patent/DE2853176A1/de not_active Ceased
- 1978-12-11 CH CH1259178A patent/CH638219A5/de not_active IP Right Cessation
- 1978-12-11 ES ES475880A patent/ES475880A1/es not_active Expired
- 1978-12-12 FR FR7834908A patent/FR2411198A1/fr active Granted
- 1978-12-12 IT IT30762/78A patent/IT1101736B/it active Protection Beyond IP Right Term
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1239814A (en) * | 1967-08-07 | 1971-07-21 | Koninklijke Gist Spiritus | Process for the preparation of 7-aminocephalosporanic acid and its derivatives |
US3839328A (en) * | 1969-11-13 | 1974-10-01 | Lilly Co Eli | Use of mixed anhydride protecting group in cleaving acyl groups from cephalosporins and penicillins |
US3931161A (en) * | 1972-03-09 | 1976-01-06 | Alfa Farmaceutici S.P.A. | Cephalosporin derivatives |
US3932392A (en) * | 1974-01-14 | 1976-01-13 | Bristol-Myers Company | Process for the preparation of 7-aminocephalosporanic acids |
US4036833A (en) * | 1974-05-28 | 1977-07-19 | Toshiyasu Ishimaru | 7-[(5'-N-methylthioacetamido)-adipoamido] cephalosporin derivatives |
Also Published As
Publication number | Publication date |
---|---|
FR2411198B1 (da) | 1982-12-17 |
GR70261B (da) | 1982-09-02 |
CH638219A5 (de) | 1983-09-15 |
GB2009736B (en) | 1982-08-18 |
ES475880A1 (es) | 1979-05-01 |
GB2009736A (en) | 1979-06-20 |
IT1101736B (it) | 1985-10-07 |
FR2411198A1 (fr) | 1979-07-06 |
DE2853176A1 (de) | 1979-06-13 |
IT7830762A0 (it) | 1978-12-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: TAKEDA CHEMICAL INDUSTRIES, LTD., 27, DOSHOMACHI 2 Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:TSUSHIMA, SUSUMU;MATSUMOTO, NORICHIKA;KATO, MASAYASU;REEL/FRAME:003859/0209 Effective date: 19790725 |
|
STCF | Information on status: patent grant |
Free format text: PATENTED CASE |