US20180014536A1 - Agent for improving plant growth and method for producing plant using same - Google Patents

Agent for improving plant growth and method for producing plant using same Download PDF

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US20180014536A1
US20180014536A1 US15/548,684 US201615548684A US2018014536A1 US 20180014536 A1 US20180014536 A1 US 20180014536A1 US 201615548684 A US201615548684 A US 201615548684A US 2018014536 A1 US2018014536 A1 US 2018014536A1
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stress
plant
agent
zerumbone
improving
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Hiroyuki Kishimoto
Shigetoyo Matsumura
Takaya NISHINOKAWA
Mari KADO
Akinori Hoshino
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Fuso Chemical Co Ltd
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Fuso Chemical Co Ltd
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Assigned to FUSO CHEMICAL CO., LTD. reassignment FUSO CHEMICAL CO., LTD. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: HOSHINO, AKINORI, KADO, MARI, KISHIMOTO, HIROYUKI, MATSUMURA, SHIGETOYO, NISHINOKAWA, TAKAYA
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal

Definitions

  • the present disclosure relates to an agent for improving plant growth which makes a plant grow healthily, and to a method for producing a plant by use of the agent.
  • the plant is subjected to various stresses due to, e.g., an environment change.
  • the plant may be subjected to temperature stress, such as high temperature stress or low temperature stress, and ultraviolet ray stress, due to drastic changes in the weather or unusual weather.
  • the plant may be subjected to dry stress due to water shortage.
  • the plant may be seriously damaged by chemical stresses caused by an agrochemical or a chemical.
  • pH stress occurs because of, e.g., root rot caused by changes in pH of soil due to acid rain or a chemical fertilizer.
  • Low oxygen stress occurs when breathing of a root is inhibited by immersion of the root in water due to a long rain or by too-hard soil.
  • An organic acid is widely used for growth promotion and disease prevention for a plant.
  • the organic acid has a high cell permeability.
  • adding an excessive amount of the organic acid to a plant gives a stress on the plant.
  • light stress encompasses infrared ray stress and low sunshine stress, each of which also inhibits growth of a plant.
  • the growth of the plant is greatly inhibited also by disease stress caused by, e.g., bacteria and/or feeding damage stress caused by an insect pest.
  • a plant growing in a seacoast area is subjected to strong salt stress. Furthermore, use of underground water for irrigation may cause a salt damage.
  • a seasonal environmental stress is a factor that limits a cultivation period. This elongates an idling period, thereby increasing a production cost. In another case, the seasonal environmental stress may be a factor that limits a species of a plant to be cultivated.
  • Patent Literatures 1 and 2 have been confirmed to have the effect of improving the plant's resistances to the environmental stresses. However, in actual cultivation of a crop, the plant may be subjected to a greater stress. Therefore, there is a demand for an agent capable of further improving the resistances. Also, there is a demand for an agent capable of improving not only the resistances to temperature stress and dry stress but also resistances to a wider range of stresses such as chemical stress and ultraviolet ray stress.
  • an active ingredient is not specified, and thus there is a concern about its safety.
  • an active ingredient is alkaloid, which is highly toxic, and thus its safety is a concern, too.
  • an object of the present disclosure is to solve the foregoing problems. Namely, the present disclosure provides an agent which gives a plant strong resistances to all various kinds of environmental stresses on the plant and which has a high safety. Alternatively, the present disclosure provides an agent which promotes plant growth or improves a plant quality.
  • An agent for improving plant growth according to the present disclosure alleviates an environmental stress on a plant, promotes plant growth, or improves a plant quality.
  • the agent for improving plant growth according to the present disclosure contains, as its main ingredient, zerumbone, an analog of zerumbone, or a salt of zerumbone or the analog.
  • the agent for improving plant growth according to the present disclosure is capable of addressing all various kinds of environmental stresses on a plant. Furthermore, the present agent for improving plant growth gives a plant a stronger stress resistance. Alternatively, the present agent for improving plant growth promotes plant growth or improves a plant quality. Furthermore, a main ingredient of the present agent for improving plant growth is a natural product contained in edible plants. Thus, this agent secures a high safety.
  • FIG. 1 shows photographs each indicating a state of cucumber seedlings in a verification experiment for anti-high temperature stress.
  • FIG. 2 shows a photograph indicating states of cucumber seedlings in a verification experiment for anti-dry stress.
  • the agent for improving plant growth alleviates various stresses on a plant, promotes plant growth, or improves a plant quality.
  • the “various stresses” herein refers to almost all kinds of stresses that may be subjected to a plant, for example, a temperature stress, a chemical stress, a light stress, a dry stress, a pH stress, a salt stress, a low oxygen stress, a feeding damage stress, and a physical stress. These stresses also include a stress caused by a disease. Namely, the present agent may also have an effect of disease prevention or improvement of a healing power.
  • the agent for improving plant growth according to the present disclosure is sesquiterpenes such as zerumbone, ⁇ -humulene, and ⁇ -caryophyllene. Sesquiterpenes are natural products contained in edible plants, and thus have a high safety.
  • Zerumbone is a type of cyclic sesquiterpene.
  • An essential oil component of wild ginger contains 80% to 90% of zerumbone.
  • ⁇ -humulene and ⁇ -caryophyllene, which is an isomer of ⁇ -humulene are analogues of zerumbone.
  • An analog has molecular biological characteristics, such as receptor binding characteristics, and a structure analogous to those of another one. Thus, the analog exhibits very similar properties to those of another one. However, the analog is a different compound from another one. Furthermore, the analog has a composition in which an atom or an atom group of a certain compound is replaced with another atom or another atom group.
  • Zerumbone, ⁇ -humulene, and ⁇ -caryophyllene can be obtained as an extract or a pulverized product of a plant of the ginger family such as ginger or turmeric.
  • zerumbone, ⁇ -humulene, and ⁇ -caryophyllene can be obtained as an extract or a pulverized product of hop, clove tree, or lavender.
  • an extract from an essential oil component of wild ginger is mainly used as zerumbone, ⁇ -humulene, and ⁇ -caryophyllene.
  • a similar efficacy can be achieved.
  • a verification experiment for high temperature stress was conducted by use of Japanese mustard spinach. The experiment was conducted in the following procedures.
  • a liquid fertilizer used was a liquid fertilizer of the Ensi (the Horticultural research station of the Ministry of Agriculture, Forestry and Fisheries of Japan) formulation, which was a general-purpose formulation for vegetable hydroponics developed by the Horticultural Experiment Station in 1960s.
  • the Japanese mustard spinach seedlings were cultured in a high temperature environment for nine days, during which growth of the seedlings were observed.
  • the agent used was zerumbone.
  • a Japanese mustard spinach seedling sprayed with sanguinarine as an agent and a Japanese mustard spinach seedling sprayed with water containing no active ingredient (hereinafter, such a seedling is called a “control sample”) were also cultured.
  • a maximum temperature during the nine days of the culture period was in a range from 30° C. to 43° C.
  • the seedlings of Japanese mustard spinach were cultured in a place with a plenty of sunlight.
  • the number of samples was 40 for each type.
  • a dry weight of 10 samples was measured at a time.
  • the reason why the Japanese mustard spinach seedling sprayed with sanguinarine was used as the comparative sample is that, among many substances, sanguinarine has an especially high effect of improving a stress resistance.
  • sanguinarine has approximately 10 times greater stress activity than the agent for giving a plant a stress resistance, especially isothianate that is known to improve a resistance to high temperature stress. Therefore, it was considered that confirming the agent's higher effect than sanguinarine would verify superiority of the agent over the other agents for giving a plant a stress resistance.
  • the average weights of the Japanese mustard spinach seedlings were compared to one another.
  • the weights of the samples sprayed with sanguinarine were merely approximately 3% greater than those of the control samples. Whether or not sanguinarine had a significant effect is unclear from this. Meanwhile, the weights of the samples sprayed with zerumbone were approximately 15% greater than those of the control samples. This clearly shows that zerumb one had an effect of improving a stress resistance.
  • the number of samples was 60 for each type. Among these, 40 samples were cultured in a place with a plenty of sunlight, whereas the other 20 samples were cultured in a place with almost no sunlight. As well as in the verification experiment 1, a maximum temperature during the nine days of the culture period was in a range from 30° C. to 43° C.
  • the average weights of the Japanese mustard spinach seedlings were compared to one another.
  • the weights of the samples sprayed with sanguinarine were as much as approximately 11% greater than those of the control samples.
  • the weights of the samples sprayed with zerumbone were as much as approximately 17% greater than those of the control samples.
  • This verification experiment was conducted under conditions almost identical to those of the verification experiment 1. Nevertheless, the samples sprayed with sanguinarine, which did not exhibit a significant effect in the verification experiment 1, exhibited a significant effect this time.
  • the samples sprayed with zerumbone exhibited better growth more stably than the control samples and the samples sprayed with sanguinarine. Meanwhile, although the samples sprayed with sanguinarine exhibited better growth than the control samples, the effect thereof was confirmed to be unstable.
  • zerumbone was confirmed to give high resistances to high temperature stress and low sunshine stress and to have an effect of growth promotion. Meanwhile, sanguinarine was also confirmed to have an effect of improving the stress resistance. However, the effect of sanguinarine was smaller than that of zerumbone. Furthermore, in a certain case, the effect of sanguinarine was hardly recognizable.
  • the culture soil contains, as fertilizer components, 180 mg/L of nitrogen, 120 mg/L of phosphoric acid, and 220 mg/L of potassium.
  • the soil was adjusted at pH 6.0. Watering was performed every early morning.
  • Zerumbone was sprayed onto the leaf surfaces of the cotyledons of cucumber seedlings three to four days after germination, and the seedlings were then cultivated for 10 days. Meanwhile, only water was sprayed onto the leaf surfaces of another cucumber seedlings, and the seedlings were then cultivated for 10 days. A growth difference was observed therebetween. Temperature and sunshine conditions were identical to those of the verification experiment 1.
  • the cucumber seedlings having the leaf surfaces sprayed with zerumbone were grown greatly. This exhibits a remarkable effect of giving a resistance to high temperature stress. Spraying zerumbone onto the leaf surfaces of the cucumber seedlings allows the seedlings to be grown greatly in all respects of the weight, the leaf area, the stem length, the root length, and the root amount.
  • Nitric acid was used here, because nitric acid is a main ingredient of general chemical fertilizers and thus the use of nitric acid does not give any effect on a plant other than acid stress.
  • Zerumbone was sprayed onto the leaf surfaces of the cotyledons of radish sprouts seedlings three to four days after germination, and the seedlings were then cultivated for several days. Meanwhile, only water was sprayed onto the leaf surfaces of another radish sprouts seedlings, and the seedlings were then cultivated for several days. The radish sprouts seedlings thus cultivated were immersed in a pH 2.5 acid solution for one minute.
  • potassium hydroxide is a main ingredient of general chemical fertilizers.
  • potassium hydroxide it is not necessary to consider any effects on a plant other than alkaline stress, and therefore it is possible to properly evaluate an efficacy regarding alkaline stress.
  • Zerumbone was sprayed onto the leaf surfaces of the cotyledons of radish sprouts seedlings three to four days after germination, and the seedlings were then cultivated for several days. Meanwhile, only water was sprayed onto the leaf surfaces of another radish sprouts seedlings, and the seedlings were then cultivated for several days. The radish sprouts seedlings thus cultivated were immersed in a pH 9.0 acid solution.
  • zerumbone gives a plant resistance to both acid and alkali.
  • Suitable pH for plant growth is in a range from approximately 5.5 to approximately 6.5. From the above results, it has turned out that zerumbone is capable of reducing the stress significantly even in an environment with pH extremely lower or higher than the suitable range.
  • FIG. 2 shows that the zerumbone-added cucumber grew more greatly than the control.
  • An average weight of the cucumber seedlings sprayed with zerumbone was approximately 13% greater than the control samples.
  • a T-test value thereof was 1.32%. This is below 5%, which is a significance level. Namely, this shows a significant difference clearly.
  • An average weight of the cucumber seedlings sprayed with ⁇ -humulene or ⁇ -caryophyllene is 8% or more greater than the control samples. T-test values thereof were below 5%, which is a significance level. Namely, this shows a significant difference clearly.
  • radish sprouts seedlings were dried so that the radish sprouts seedlings were severely damaged. Subsequently, adequate water was given to the radish sprouts seedlings. Thus, whether or not the radish sprouts seedlings could be recovered from the damage was verified.
  • radish sprouts were immersed in water during night so that germination thereof was promoted. After germination, radish sprouts seedlings planted in vermiculite were impregnated in a nutrient fluid of the Ensi formulation. The radish sprouts seedlings were cultured at 25° C. for five days. A light period during the culture was six hours. Then, zerumbone was sprayed to the radish sprouts seedlings. Meanwhile, water was sprayed to samples used as a control.
  • radish sprouts seedlings were cultured for one more day. Thereafter, the roots thereof were washed so that the vermiculite was removed therefrom. Then, the roots were wiped with a paper towel. The radish sprouts seedlings were then arranged on a tray, and were dried for one night.
  • the radish sprouts seedlings thus dried were planted in culture soil obtained by blending leaf soil and Akadama soil in a ratio of 4:6 in a pot.
  • the verification experiments 6 and 7 show that the use of zerumbone can adequately alleviate dry stress on a plant. Not only this, the verification experiments 6 and 7 show that the use of zerumbone has an effect of recovering the damage given by dry stress.
  • Cucumber was used as a plant.
  • cucumber seeds were immersed in water of 25° C. for 48 hours. Thereafter, two seeds were sown in a cultivation pot. At the time of sowing, 3 ml of a respective liquid agent was added thereto. Then, in the sunlight, the seeds were cultivated for 17 days under a temperature condition of approximately 30° C. A respective liquid agent was sprayed onto the whole leaf surfaces of grown cucumber seedlings. Furthermore, under conditions identical to the above, the cucumber seedlings were cultivated for one day. As a control sample, a cucumber seedling to which only water was added and onto which only water was sprayed was also cultivated under conditions identical to the above.
  • the second leaf from the top was collected from each sample. Then, the leaves thus collected were cut in an almost equal size. The leaves thus cut were put into poly bags together with tap water, and then the poly bags were tightly closed. Then, the poly bags were left at rest for two days in a dark place cooled at ⁇ 10° C. or 0° C.
  • TTC test (2,3,5-triphenyl tetrazolium chloride test) was conducted.
  • TTC test is a technique for determining an activity of a cell. According to this technique, a cell having a higher activity is dyed redder, whereas a dead cell is hardly dyed.
  • the control sample applied with no agent was dyed slightly. Namely, a part of the cells in the control sample had a weak activity. Meanwhile, the samples applied respectively with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene as the agents were dyed deeply. Namely, it was confirmed that almost all the cells in these samples had a strong activity.
  • the second leaf from the top was collected from each sample as a sample for low temperature stress.
  • the remaining parts of the samples were further cultivated for two days under a fluorescent lamp in a cool place at 4° C. Then, TTC test was conducted on these samples.
  • the above result of the experiment shows that the agents of zerumbone, ⁇ -humulene, ⁇ -caryophyllene are capable of giving cucumber a high resistance to low temperature stress.
  • the verification experiment 4 verified the resistance to general acid stress, i.e., a low pH environment, with use of nitric acid, which is an inorganic acid. Meanwhile, acetic acid, which is an organic acid, was used in this verification experiment.
  • organic acids such as acetic acid are widely used for growth promotion and disease prevention for a crop.
  • Pyroligneous acid (a supernatant in a liquid produced by destructive distillation of woods), which is used in organic farming, also contains acetic acid as its main ingredient.
  • organic acid has a high cell permeability. Therefore, if a too high concentration of organic acid is given to a plant, a cell of the plant will be damaged.
  • acetic acid has the smallest molecular weight among the organic acids.
  • acetic acid has a small dissociation rate. Therefore, acetic acid easily penetrates a cell to give a serious damage to the cell.
  • each sample was immersed in a 0.4% acetic acid solution for two hours. On such samples, TTC test was conducted.
  • the above agents improve a resistance to acetic acid, which has the highest cell permeability. From this, it is considered that the above agents are capable of improving resistances to other organic acids adequately.
  • Inorganic nitrogen exists in soil in three forms of ammonia-nitrogen, nitrite-nitrogen, and nitrate nitrogen. Typically, once an organic matter is decomposed, ammonia-nitrogen is first formed. Furthermore, due to effects of nitrate bacteria and/or the like in the soil, ammonia-nitrogen is converted to nitrite-nitrogen, and then to nitrate nitrogen. Ideally, nitrate nitrogen, which is nontoxic, is applied to a plant. However, due to too much fertilization and/or the like, ammonia-nitrogen, which is toxic, may be absorbed in a plant. Therefore, it is important to improve a resistance to ammonia for healthy growth of a plant.
  • the ultraviolet ray has a shorter wavelength (i.e., a greater energy per photon) than those of visible light and an infrared ray. Therefore, the ultraviolet ray gives a significantly severe damage to a living organism.
  • This verification experiment used some of the samples that were cut in the almost equal size in the verification experiment 8.
  • the samples were floated in water in petri dishes, and were irradiated with an ultraviolet ray for 24 hours. On such samples, TTC test was conducted.
  • the above agents were effective against the ultraviolet ray, which is the severest.
  • the above agents are capable of improving resistances to all kinds of light stress given to a plant in a normal environment.
  • each of zerumbone, ⁇ -humulene, and ⁇ -caryophyllene is capable of giving a plant resistances to various stresses of organic acid stress, ammonia stress, light stress, and salt stress.
  • the vinyl greenhouse was a pipe-structure vinyl greenhouse.
  • a deep flow technique device was set, and hydroponics was conducted.
  • the deep flow technique is one technique for hydroponics. According to this technique, a nutrient fluid containing a fertilizer dissolved therein is accumulated in a cultivation bed, and a crop is cultivated only with the nutrient fluid, without using soil.
  • the cultivation was performed for approximately one month in summer.
  • a maximum temperature was in a range from 28° C. to 44° C.
  • a minimum temperature was in a range from 24° C. to 27° C.
  • Leaf lettuce was deliberately selected as a crop to be cultivated, although the leaf lettuce has its growth optimum temperature of 18° C. to 23° C. and, therefore, grows suitably in a cool climate. Since the leaf lettuce has a low tolerance for heat, the leaf lettuce is likely to suffer from various physiological disorders such as unproductive growth, tip burn (tip burn disease), and heterophyll and various diseases such as powdery mildew and root rot. Thus, cultivating the leaf lettuce in summer is generally considered to be difficult.
  • Seeds were sown on culture sponge spread on a seedling tray. One day after the sowing, germination was observed.
  • the sponge was cut into pieces such that each of the pieces had one seedling.
  • the pieces of sponge were set in a styrene foam float such that the seedlings were spaced from each other by 10 cm.
  • the float was floated in the deep flow technique device.
  • a liquid fertilizer a liquid fertilizer of the Ensi formulation was used.
  • a pH adjuster sodium hydroxide and phosphoric acid were used. During the cultivation period, a pH value was maintained within a range of 6.0 to 6.5.
  • the leaf lettuce seedlings were transplanted to a styrene foam float such that the seedlings were spaced from each other by 25 cm.
  • the float was floated in the deep flow technique device.
  • the liquid fertilizer and the pH value were equal to those described above.
  • the leaf lettuce seedlings were harvested. The leaf lettuce seedlings were observed regarding whether or not a physiological disorder or a disease occurred.
  • the five items regarding the physiological disorder include heterophyll, unproductive growth, tip burn, a root spread condition, and taste
  • the two items regarding the disease resistance include powdery mildew and root rot.
  • the harvest timing at which the harvest size reached the range of 150 g to 180 g was the 38th day in the control test section, and was the 35th day in the sanguinarine test section. This shows that the growing speed was improved by the spraying of sanguinarine. Meanwhile, in the zerumbone test section, the harvest size reached the range of 150 g to 180 g on the 30th day after the germination. Furthermore, the harvest timing was the 32nd day in the ⁇ -humulene test section, and was the 33rd day in the ⁇ -caryophyllene test section. Thus, it was confirmed that the growing speed was improved by the spraying of zerumbone, ⁇ -humulene, or ⁇ -caryophyllene, as compared to the case where sanguinarine was sprayed.
  • the heterophyll, the unproductive growth, and the tip burn occurred in the control test section. Further, in this section, the root spread condition was also poor. Also in the sanguinarine test section, the heterophyll and the unproductive growth occurred. Meanwhile, in the test sections sprayed respectively with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene, no physiological disorder occurred. Further, in these test sections, the root spread condition was far better than in the sanguinarine test section.
  • the leaf lettuce seedling in the control test section had bitterness. This is assumed to be caused by excess production of lactucopirin, which is a bitter substance. Meanwhile, the leaf lettuce seedlings in the test sections respectively sprayed with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene had no bitterness.
  • Embodiment 1 the description has been given regarding the results of the experiments for verifying effectiveness of the agent for improving plant growth with respect to Spermatophyta.
  • the present embodiment will describe results of experiments for verifying effectiveness of the agent for improving plant growth with respect to Pteridophyta.
  • autumn fern As a Pteridophyta, autumn fern was used, which is a representative Pteridophyta often found in, e.g., a glassland and a bright forest. Verification experiments for various stresses were conducted thereon. The experiments were conducted in the following procedures.
  • the whole leaf surfaces of autumn fern seedlings that were grown sufficiently were sprayed respectively with the agents of zerumbone, ⁇ -humulene, and ⁇ -caryophyllene. Meanwhile, the leaf surfaces of the control sample were sprayed only with water. After the spraying onto the leaf surfaces, the autumn fern seedlings were cultivated at 25° C. for one day. The leaves were cut in a suitable size, and were then subjected to various stresses. Then, cell activities of the samples were determined by TTC test.
  • the leaves that were cut in the suitable size were immersed in tap water of 45° C. for 18 hours. On such leaves, TTC test was conducted.
  • the control sample applied with no agent was not dyed. Namely, almost all the cells therein were dead. Meanwhile, all the samples applied respectively with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene as the agents were dyed slightly. Namely, the cells therein had an activity. Thus, these cells were confirmed to be alive.
  • the leaves that were cut in the suitable size were immersed in a 0.4% acetic acid solution for three hours. On such leaves, TTC test was conducted.
  • the control sample applied with no agent was not dyed. Namely, almost all the cells therein were dead. Meanwhile, all the samples applied respectively with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene as the agents were dyed slightly. Namely, the cells therein had an activity. Thus, these cells were confirmed to be alive.
  • the control sample applied with no agent was not dyed. Namely, almost all the cells therein were dead. Meanwhile, all the samples applied respectively with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene as the agents were dyed slightly. Namely, the cells therein had an activity. Thus, these cells were confirmed to be alive.
  • Embodiments 1 and 2 the descriptions have been given regarding the results of the experiments for verifying effectiveness of the agent for improving plant growth with respect to Spermatophyta and Pteridophyta.
  • the present embodiment will describe results of experiments for verifying effectiveness of the agent for improving plant growth with respect to Bryophyta.
  • racomitrium moss As a Bryophyta, racomitrium moss was used, which is a Bryophyta widely used for greening a park, a rooftop, and a garden. Verification experiments for various stresses were conducted thereon. The experiments were conducted in the following procedures.
  • Racomitrium moss mats were sprayed respectively with the agents of zerumbone, ⁇ -humulene, and ⁇ -caryophyllene. Meanwhile, the control sample was sprayed only with water. After the spraying, the racomitrium mosses were cultivated at 25° C. for one day. Then, the samples were subjected to various stresses. Thus, cell activities of the samples were determined by TTC test.
  • the samples were immersed in tap water of 45° C. for 18 hours. On such samples, TTC test was conducted.
  • the control sample applied with no agent was not dyed. Namely, almost all the cells therein were dead. Meanwhile, all the samples applied respectively with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene as the agents were dyed slightly. Namely, the cells therein had an activity. Thus, these cells were confirmed to be alive.
  • the samples were immersed in a 0.4% acetic acid solution for three hours. On such samples, TTC test was conducted.
  • control sample applied with no agent was not dyed. Namely, almost all the cells therein were dead. Meanwhile, all the samples applied respectively with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene as the agents were dyed slightly.
  • the cells therein had an activity. Thus, these cells were confirmed to be alive.
  • the samples were immersed in a 1.0% ammonia solution for 48 hours. On such samples, TTC test was conducted.
  • the control sample applied with no agent was not dyed. Namely, almost all the cells therein were dead. Meanwhile, all the samples applied respectively with zerumbone, ⁇ -humulene, and ⁇ -caryophyllene as the agents were dyed slightly. Namely, the cells therein had an activity. Thus, these cells were confirmed to be alive.
  • the agent for improving plant growth according to the present disclosure alleviates various stresses on a plant. Furthermore, the present agent for improving plant growth also has an effect of improving a power of recovery from a damage when it is given to a plant due to stress.
  • the present embodiment will describe a method for effectively producing, by taking advantage of the above characteristics, a crop with a high content of a functional component.
  • a crop with a high content of a functional component can be produced by causing a plant to directly take in the functional component from its root and leaf.
  • a plant in order to cause the plant to take in the functional component, it is necessary to expose the plant to a high-concentration functional component. Consequently, the plant is damaged (chemical stress) by the functional component itself.
  • the agent for improving plant growth By preliminarily adding to a plant the agent for improving plant growth, the resistances to the various stresses are improved. Consequently, the first method achieves an expanded range of choices for a kind of stress to be given to a plant and a way to give the stress. Thus, it is possible to produce a plant with a higher amount of a functional component.
  • the second method achieves an expanded range of choices for a functional component that can be taken into a plant.
  • the second method allows causing a plant to take in a larger amount of a functional component more efficiently.
  • the present agent for giving a plant a stress resistance is sesquiterpenes such as zerumbone, ⁇ -humulene, and ⁇ -caryophyllene.
  • the sesquiterpenes are natural products contained in edible plants.
  • the present agent for giving a plant a stress resistance secures a high safety. Therefore, the present agent for giving a plant a stress resistance can be used for a crop to be eaten by a human without anxiety.
  • the present agent for giving a plant a stress resistance has an effect of improving resistances to all various stresses that may be subjected to a plant, and therefore has a quite high utility value.
  • plants have their growth optimum temperatures.
  • the plants have respective specific temperature ranges in which the plants can grow, namely, respective regions where the plants can grow.
  • use of the present agent improves resistances to high temperature stress and low temperature stress. This makes it possible to grow a plant even in a high or low temperature region which is outside the suitable temperature region. Namely, this makes it possible to expand a range of choices for a plant to be grown in each region.
  • the present agent for giving a plant a stress resistance is also capable of improving a resistance to dry stress. This makes it possible to expand a range of choices for a plant to be grown in an arid region.
  • the present agent is also capable of improving a resistance to salt stress. This makes it possible to expand a range of choices for a plant to be grown in a coastal region.
  • the present agent may also alleviate pH stress and ammonia stress. This makes it possible to grow a wide variety of plants independently of a quality of soil.
  • the present agent for giving a plant a stress resistance is capable of improving other various environmental stress resistances. Therefore, even with extensive cultivation in which sufficient management is not performed, it is possible to improve the quality and yield. This makes it possible to reduce a management cost in agriculture.
  • the present agent is capable of improving resistances to various stresses that may occur in an artificial cultivation environment such as a plant factory or horticulture under structure. This makes it possible to improve the quality and yield.
  • the present agent for improving plant growth results in improvement of a systemic acquired resistance of the plant.
  • the plant is capable of resisting therewith. This leads to reduction in amounts of agrochemicals.
  • the present agent for improving plant growth has not only an effect of alleviating a stress but also an effect of improving a power of recovery from a damage.
  • the present agent is capable of improving a plant's natural healing power from various diseases.
  • a pathogen that causes harm to a plant is exterminated by use of a chemical method such as a drug, an acid, or an alkali and/or a physical method such as heat, an ultraviolet ray, or drying.
  • a chemical method such as a drug, an acid, or an alkali
  • a physical method such as heat, an ultraviolet ray, or drying.
  • the resistances to pH stress and organic acid stress can be improved by the present agent. This expands a range of choices for an agent available for disease prevention and extermination of a pathogen and/or the like.
  • a plant is cultivated for rooftop greening or wall greening
  • the plant is exposed to various kinds of strong stress.
  • the plant In its special environment with extremely little soil, the plant is always exposed to (high and low) temperature stress, dry stress, and light stress.
  • a buffer action in soil does not function.
  • pH and a chemical substance concentration are likely to have extreme values. Consequently, the plant is subjected to strong pH stress and chemical stress.
  • breakout of an insect causing a biting damage such as a gold beetle larva is likely to occur. In such a severe environment for the plant, the plant is cultivated.
  • a plant is often subjected to plural various stresses.
  • plural different agents are applied to the plant to improve resistances to the respective stresses, interaction between the agents is a matter of concern.
  • use of the agent for improving plant growth according to the present disclosure which is effective for the various kinds of stress, eliminates such concern.
  • the functional crop refers to a crop containing a high content of a functional component that improves a biological regulation function of a human.
  • the present agent for improving plant growth is effective.
  • the first method achieves an expanded range of choices for a kind of stress to be given and a way to give the stress. Furthermore, it is possible to produce a plant containing a larger amount of a functional component. Meanwhile, the second method achieves a wider range of choices for a functional component that can be taken into a plant. In addition, the second method allows for letting plants to take a larger amount of functional components more efficiently.
  • zerumbone, ⁇ -humulene, or ⁇ -caryophyllene has an effect of alleviating an environmental stress on a plant, promoting plant growth, or improving the quality. Furthermore, it was confirmed that these substances are suitable for new use as an agent for improving plant growth.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)
US15/548,684 2015-02-06 2016-02-04 Agent for improving plant growth and method for producing plant using same Abandoned US20180014536A1 (en)

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JP2015021848A JP6789612B2 (ja) 2015-02-06 2015-02-06 植物用発育向上剤、およびそれを用いた植物の製造方法
PCT/JP2016/053431 WO2016125875A1 (ja) 2015-02-06 2016-02-04 植物用発育向上剤、およびそれを用いた植物の製造方法

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Cited By (3)

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Publication number Priority date Publication date Assignee Title
CN110301183A (zh) * 2019-06-27 2019-10-08 华南农业大学 一种食用白醋促进构树种子萌发的方法
KR20240161512A (ko) 2023-05-04 2024-11-12 씨제이제일제당 (주) 아미노산을 포함하는 염 스트레스 경감용 조성물
KR20240161513A (ko) 2023-05-04 2024-11-12 씨제이제일제당 (주) 아미노산을 포함하는 저온 스트레스 경감용 조성물

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JPWO2024038805A1 (enExample) 2022-08-18 2024-02-22

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JPH05201821A (ja) * 1991-06-11 1993-08-10 Nippon Getsutou Kk 防虫抗菌性物質
JP3205446B2 (ja) * 1993-09-22 2001-09-04 高砂香料工業株式会社 植物成長促進剤及び植物成長促進方法
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110301183A (zh) * 2019-06-27 2019-10-08 华南农业大学 一种食用白醋促进构树种子萌发的方法
KR20240161512A (ko) 2023-05-04 2024-11-12 씨제이제일제당 (주) 아미노산을 포함하는 염 스트레스 경감용 조성물
KR20240161513A (ko) 2023-05-04 2024-11-12 씨제이제일제당 (주) 아미노산을 포함하는 저온 스트레스 경감용 조성물

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US20190150436A1 (en) 2019-05-23
US10834923B2 (en) 2020-11-17

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