US20140287403A1 - Nanoscale motion detector - Google Patents
Nanoscale motion detector Download PDFInfo
- Publication number
- US20140287403A1 US20140287403A1 US14/350,451 US201214350451A US2014287403A1 US 20140287403 A1 US20140287403 A1 US 20140287403A1 US 201214350451 A US201214350451 A US 201214350451A US 2014287403 A1 US2014287403 A1 US 2014287403A1
- Authority
- US
- United States
- Prior art keywords
- motion detector
- support
- cantilever
- sensor
- displacement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/533—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isomerase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/02—Analysing fluids
- G01N29/022—Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/02—Analysing fluids
- G01N29/036—Analysing fluids by measuring frequency or resonance of acoustic waves
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/22—Details, e.g. general constructional or apparatus details
- G01N29/24—Probes
- G01N29/2418—Probes using optoacoustic interaction with the material, e.g. laser radiation, photoacoustics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/44—Processing the detected response signal, e.g. electronic circuits specially adapted therefor
- G01N29/46—Processing the detected response signal, e.g. electronic circuits specially adapted therefor by spectral analysis, e.g. Fourier analysis or wavelet analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y35/00—Methods or apparatus for measurement or analysis of nanostructures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2291/00—Indexing codes associated with group G01N29/00
- G01N2291/04—Wave modes and trajectories
- G01N2291/042—Wave modes
- G01N2291/0427—Flexural waves, plate waves, e.g. Lamb waves, tuning fork, cantilever
Definitions
- the present invention relates to the analysis at a very low scale of objects having a size ranging from Angstroms to micrometers. It particularly but not exclusively relates to the detection of the movement or the inner dynamics of said objects.
- the present invention concerns a motion detector as defined in the claims.
- the detector comprises a flexible support adapted to hold at least one object (for instance an object having a movement or an intrinsic dynamics), a sensor for measuring the displacement said support and processing means for differentiating the movement of said support from the one induced by the said object.
- object for instance an object having a movement or an intrinsic dynamics
- processing means for differentiating the movement of said support from the one induced by the said object.
- the invention also concerns a method for analysing small sized specimens, ranging typically from Angstroms to micrometers.
- the method according to the invention can be used in any kind of environment, vacuum, air, liquid or physiological medium.
- fluctuation encompasses any type of motion, in particular the vibration and the deflection.
- the invention may be particularly useful for detecting the movement or intrinsic dynamics (or absence of them) of objects such as proteins, lipids, nucleic acids, glucides, viruses, bacteria or cells in presence or absence of external or internal stimuli.
- the induced displacement of the said support may occur by the movement of the object, by the thermal fluctuations induced by the object, by changes in the interaction between the object and the flexible support, by the internal dynamics of the object or by any physical, chemical or biological phenomenon generated by the object and provoking the fluctuations of the said support.
- FIG. 1 illustrates a first setup according to the invention for sensing movement at the nanoscale.
- FIG. 2 illustrates another setup according to the invention for sensing movement at the nanoscale.
- FIG. 3 illustrates a theoretical molecular structure of human TopoII and a setup according to the invention for observing TopoII drug interactions.
- FIG. 4 represents an analysis (deflection and variance) of the interaction of human Topoisomerase II with ATP and aclarubicin (ACLAR).
- FIG. 5 represents an analysis (deflection and variance) of the interaction of human Topoisomerase II with different concentrations of ATP.
- FIG. 6 represents an analysis (deflection and variance) of the interaction of human TopoII with supercoiled DNA.
- FIG. 7 illustrates another setup according to invention for observing bacteria viability following exposure to chemical and/or physical stimuli.
- FIG. 8 represents an analysis (deflection and variance) of the resistance of E. coli to antibiotics.
- FIG. 9 represents a dose dependent analysis (variance values) with a detector according to the invention.
- FIG. 10 represents an analysis (deflection and variance) of the exposure of Staphylococcus aureus bacteria to ampicillin.
- FIG. 1 represents a first embodiment of the invention with a motion detector comprising a cantilever 1 , a laser beam 2 , a minor 3 and two or four segments photodiodes 4 .
- FIG. 2 represents a similar assembly but where the cantilever is replaced by an optical fibre 5 and where the laser beam 2 is collimated in the fibre 5 and is collected towards its free end.
- One or several movable objects ( 6 - 9 )—see also FIGS. 3 , 6 and 7 —to be investigated are positioned on a microsized flexible support 1 , 5 .
- the fluctuations of the support 1 are recorded as a function of time.
- the method offers the advantage to monitor the evolution of the dynamics of the object(s), for instance as exposed to chemical or physical modifications of the environment.
- the system may be advantageously made of one or several fluctuating supports, an analysis chamber in which the supports are introduced and a transduction system that detects and records the support movements.
- the support 1 , 5 may be a cantilever 1 , such as those used in atomic force microscopy (AFM) (see FIGS. 1 , 3 , 6 and 7 ), an optical fibre 5 , a piezoelectric system (not illustrated), a membrane or any microdevice capable of fluctuating. It has to be optimized to allow the attachment of the object on its surface by any means, for instance using chemical, biological or physical methods.
- AFM atomic force microscopy
- the objects 6 - 9 can range from single molecules to complex specimens such as nanodevices, proteins, DNA, viruses, bacteria, single cells or complex multicellular systems.
- the analysis chamber preferably comprises a single or multiple inlets, a space containing the sensor and the object and one or several outlets, in order to permit exposure of the object(s) to different environmental conditions.
- the transduction system e.g. the photodetector 4 , detects the fluctuations of the objects 6 - 9 through the support 1 , 5 fluctuations. It can be based on, but not limited to, optical reflection, optical interference, piezo electric, electric, magnetic, capacitive or tunneling detection systems. As examples similar systems are typically employed in AFM microscopy, microbalances or accelerometers.
- the data collected by the transduction system may be advantageously analysed by a dedicated electronics optimized to highlight the dynamical component of the signal, by performing any kind of manipulation capable to evidence the variation in the object dynamics.
- the fluctuating detector is first processed in a way to promote the attachment of the objects 6 - 9 .
- the support 1 , 5 is exposed to the objects 6 - 9 .
- This procedure can be carried on in or outside an analysis chamber.
- different working conditions are produced in the analysis chamber by modifying the chemical or physical environment around the specimen.
- the conformational changes of the specimen or its motions, during all the described steps, induce fluctuations that are translated in measurable (electric) signals by the sensor and are recorded by the dedicated electronics.
- the data are finally analysed by dedicated algorithms to highlight the insurgence or modification of the specimen's movements.
- Topoisomerase II (TopoII— FIG. 3 ) and its interaction with anticancerous drugs.
- TopoII is an essential enzyme that interacts with DNA to simplify its topology and permits the transcription to occur safely.
- TopoII is also the preferred target of numerous anticancerous drugs such as aclarubicin. This drug binds to TopoII, freezes its conformation and inhibits its action ( 15 ).
- TopoII was adsorbed onto both sides of a cantilever. It was than introduced in the analysis chamber of an AFM and its laser beam was collimated on the apex of the cantilever. The reflection of the laser beam, sent to a split photo-detector, allowed detecting the fluctuations of the cantilever as depicted in FIG. 1 and, more in detail, FIG. 3 .
- the experiment consisted in injecting successively an ATP depleted buffer, an ATP enriched solution and an aclarubicin+ATP rich media in the analysis chamber and by recording the resulting fluctuations of the cantilever.
- This experiment was performed using an APTES-coated AFM cantilever.
- the different buffers injected during experiment are: buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM MgCl2 and 0.5 mM dithiothreitol), 0.02 mM ATP and 0.02 mM ATP plus 100 ⁇ M aclarubicin.
- the top panel shows the cantilever deflection data, while the bottom evidences the differences of the cantilever fluctuation in terms of variance.
- TopoII molecules by ATP were dependent on its concentration, as shown in FIG. 5 .
- the media used in this experiment are: buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM MgCl 2 and 0.5 mM dithiothreitol), 0.02 mM ATP, 0.2 mM ATP, 2 mM ATP and again buffer.
- the top panel shows the cantilever deflection data, while the bottom one evidences the differences of the cantilever fluctuation in terms of variance.
- TopoII interacts with DNA to simplify its topology.
- TopoII-supercoiled DNA complexes on both sides of an AFM cantilever, as depicted in FIG. 6 .
- the experiment was performed using an APTES-coated AFM cantilever.
- the different media injected during experiment are: buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM MgCl 2 and 0.5 mM dithiothreitol), 0.02 mM AMPPNP (an ATP analogue) and 0.02 mM ATP.
- Panel A shows the cantilever deflection data.
- Panel B depicts the experimental set-up to follow TopoII-DNA interactions. a) DNA molecule, b) TopoII, c) AFM cantilever, d) laser beam.
- Panel C evidences the differences in terms of the variance.
- the presented method is sensitive enough to detect external as well as internal motion of bacteria and can be used to assess the action of antibacterial agents.
- This experiment shows the capability to explore the sensibility of bacteria to antibiotics with a very high temporal resolution ranging between seconds and minutes.
- Motile bacteria Escherichia coli
- resistant to kanamycin but sensitive to ampicillin were adsorbed to both sides of a cantilever.
- the bacteria were successively exposed to both antibiotics to determine the impact on the bacterial motion/viability (see FIG. 7 ).
- the cantilever was introduced in the analysis chamber and exposed to a solution containing bacteria that eventually attached to its surface. The system was then exposed to: 1) nutriment depleted buffer, 2) nutriment solution (Lysogeny broth (LB)), 3) kanamycin-enriched LB solution 4) LB solution 5) ampicillin-enriched LB solution and, finally, 6) LB solution.
- LB Lysogeny broth
- step 2 It appeared that the bacterial motion increased during step 2), diminished when exposed to kanamycin at step 3), increased again in the presence of nutriment at step 4) dramatically decreased in the presence of ampicillin at step 5) and remained at the same value despite the presence of the nutrient solution at step 6).
- FIG. 8 depicts the evolution of the cantilever fluctuations and of its STD during the different phases of the experiment.
- the experiment was performed using an APTES-coated AFM cantilever.
- the different phases of the experiment are depicted: PBS, bacteria in PBS, bacteria in LB, exposure to kanamycin, washing with LB, exposure to ampicillin, washing with LB.
- the top panel shows the cantilever deflection data, while the bottom one evidences the differences in terms of the variance.
- the present invention provides a device and a method that detect motion of nano to micrometer sized systems with a high spatial and temporal resolution.
- the method can be used to (but is not limited to) monitoring conformational changes of single molecules, biochemical reactions, drug-target interactions as well as internal and external motions of cells and bacteria. Due to its high sensitivity to movement, it can be used as a detector of life presence in extreme environments (e.g. extra-terrestrial environments).
- the procedure improves the existing technology ( 16 - 24 ) by evidencing easily and quantitatively even the slightest fluctuation of the motion detector and can be utilized in any kind of environment, especially in physiological medium.
- the achievable fluctuation and temporal resolution permits to predict its potential application to a vast number of fields, such as (but not limited to) cellular and molecular biology, bacteriology, microbiology, drug development, high-speed pharmaceutical evaluation, or molecule conformational monitoring.
- fields such as (but not limited to) cellular and molecular biology, bacteriology, microbiology, drug development, high-speed pharmaceutical evaluation, or molecule conformational monitoring.
- this technique to slow growing bacteria, such as Mycobacterium tuberculosis.
- the operating principle is extremely simple and the required materials are standard and completely reusable (electronics, microfluidics, mechanics), a device based on such invention has very low manufacturing and maintenance costs.
- it can be easily parallelized by combining several sensors in order to improve measurement throughput and reliability.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Signal Processing (AREA)
- Acoustics & Sound (AREA)
- Toxicology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Optics & Photonics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Mathematical Physics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Strength Of Materials By Application Of Mechanical Stress (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IBIB2011/054553 | 2011-10-14 | ||
IB2011054553 | 2011-10-14 | ||
PCT/IB2012/055564 WO2013054311A1 (en) | 2011-10-14 | 2012-10-12 | Nanoscale motion detector |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2012/055564 A-371-Of-International WO2013054311A1 (en) | 2011-10-14 | 2012-10-12 | Nanoscale motion detector |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/957,694 Division US11299762B2 (en) | 2011-10-14 | 2018-04-19 | Nanoscale motion detector |
Publications (1)
Publication Number | Publication Date |
---|---|
US20140287403A1 true US20140287403A1 (en) | 2014-09-25 |
Family
ID=47297332
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US14/350,451 Abandoned US20140287403A1 (en) | 2011-10-14 | 2012-10-12 | Nanoscale motion detector |
US15/957,694 Active 2033-12-18 US11299762B2 (en) | 2011-10-14 | 2018-04-19 | Nanoscale motion detector |
US17/693,682 Pending US20220195489A1 (en) | 2011-10-14 | 2022-03-14 | Nanoscale motion detector |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/957,694 Active 2033-12-18 US11299762B2 (en) | 2011-10-14 | 2018-04-19 | Nanoscale motion detector |
US17/693,682 Pending US20220195489A1 (en) | 2011-10-14 | 2022-03-14 | Nanoscale motion detector |
Country Status (8)
Country | Link |
---|---|
US (3) | US20140287403A1 (tr) |
EP (1) | EP2766722B1 (tr) |
JP (2) | JP2014528591A (tr) |
DK (1) | DK2766722T3 (tr) |
ES (1) | ES2710191T3 (tr) |
PL (1) | PL2766722T3 (tr) |
TR (1) | TR201901893T4 (tr) |
WO (1) | WO2013054311A1 (tr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10947576B2 (en) | 2017-07-25 | 2021-03-16 | Arizona Board Of Regents On Behalf Of Arizona State University | Rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160299132A1 (en) | 2013-03-15 | 2016-10-13 | Ancera, Inc. | Systems and methods for bead-based assays in ferrofluids |
WO2016210348A2 (en) | 2015-06-26 | 2016-12-29 | Ancera, Inc. | Background defocusing and clearing in ferrofluid-based capture assays |
NL2024356B1 (en) | 2019-12-02 | 2021-08-31 | Univ Delft Tech | 2D material detector for activity monitoring of single living micro-organisms and nano-organisms |
EP4081649A1 (en) | 2019-12-23 | 2022-11-02 | Resistell AG | Attachment of biological and non-biological objects, e.g. bacterial cells, to surfaces, e.g cantilevers |
NL2031130B1 (en) | 2022-03-02 | 2023-09-11 | Univ Delft Tech | Clinical sample preparation and handling for activity monitoring of single living micro- and nano-organism |
WO2023174728A1 (en) | 2022-03-15 | 2023-09-21 | Resistell Ag | Method of analysing the motional activity of particles |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090053709A1 (en) * | 2007-02-16 | 2009-02-26 | Drexel University | Enhanced sensitivity of a cantilever sensor via specific bindings |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0835976A (ja) * | 1994-07-21 | 1996-02-06 | Olympus Optical Co Ltd | 集積型spmセンサーおよび変位検出回路 |
US6289717B1 (en) | 1999-03-30 | 2001-09-18 | U. T. Battelle, Llc | Micromechanical antibody sensor |
ATE309042T1 (de) * | 1999-05-03 | 2005-11-15 | Cantion As | Sensor für ein mikrofluidisches bearbeitungssystem |
JP2001013155A (ja) * | 1999-07-02 | 2001-01-19 | Seiko Instruments Inc | 走査型プローブ顕微鏡の測定方法および装置 |
US7148017B1 (en) | 2000-07-12 | 2006-12-12 | Cornell Research Foundation, Inc. | High sensitivity mechanical resonant sensor |
TWI220423B (en) | 2001-08-30 | 2004-08-21 | Hrl Lab Llc | A method of fabrication of a sensor |
US20030068655A1 (en) | 2001-09-12 | 2003-04-10 | Protiveris, Inc. | Microcantilever apparatus and methods for detection of enzymes |
US20060121502A1 (en) | 2001-11-09 | 2006-06-08 | Robert Cain | Microfluidics apparatus for cantilevers and methods of use therefor |
DE10209245B4 (de) | 2002-03-04 | 2005-12-22 | Concentris Gmbh | Vorrichtung und Verfahren zur Detektion von Mikroorganismen |
CA2479861A1 (en) | 2002-03-20 | 2003-10-02 | Purdue Research Foundation | Microscale sensor element and related device and method of manufacture |
WO2004038762A2 (en) | 2002-10-21 | 2004-05-06 | Alegis Microsystems | Nanomotion sensing system and method |
US7207206B2 (en) * | 2004-02-19 | 2007-04-24 | Ut-Battelle, Llc | Chemically-functionalized microcantilevers for detection of chemical, biological and explosive material |
JP2005348651A (ja) * | 2004-06-10 | 2005-12-22 | Nagasaki Prefecture | 有機・無機系抗菌剤のマイクロプレート殺菌力試験方法 |
CN101198866A (zh) | 2005-05-20 | 2008-06-11 | 皇家墨尔本理工大学 | 化验装置 |
JP2006337249A (ja) * | 2005-06-03 | 2006-12-14 | Canon Inc | カンチレバーセンサを利用するターゲット物質の検出方法及び検出装置 |
JP2007003234A (ja) | 2005-06-21 | 2007-01-11 | Mitsubishi Chemicals Corp | カンチレバーを用いた検出対象物質の検出方法及びカンチレバーセンサシステム |
US7671511B2 (en) | 2006-12-12 | 2010-03-02 | Concentris Gmbh | System for oscillating a micromechanical cantilever |
JP4967062B2 (ja) * | 2007-08-22 | 2012-07-04 | ホンダ リサーチ インスティテュート ヨーロッパ ゲーエムベーハー | オプティカルフロー、運動学及び深さ情報を使用して、物体の適切な運動を推定する方法 |
US8236508B2 (en) | 2008-01-29 | 2012-08-07 | Drexel University | Detecting and measuring live pathogens utilizing a mass detection device |
TR201201780T2 (tr) | 2009-08-20 | 2012-03-21 | K�Lah Haluk | Mikroelektromekanik sistem (MEMS) teknolojisi ile üretilmiş, mikroakışkan-kanal içine gömülebilir, yatay eksende salınan gravimetrik sensör aygıtı. |
-
2012
- 2012-10-12 US US14/350,451 patent/US20140287403A1/en not_active Abandoned
- 2012-10-12 DK DK12797978.9T patent/DK2766722T3/en active
- 2012-10-12 EP EP12797978.9A patent/EP2766722B1/en active Active
- 2012-10-12 JP JP2014535222A patent/JP2014528591A/ja active Pending
- 2012-10-12 ES ES12797978T patent/ES2710191T3/es active Active
- 2012-10-12 PL PL12797978T patent/PL2766722T3/pl unknown
- 2012-10-12 TR TR2019/01893T patent/TR201901893T4/tr unknown
- 2012-10-12 WO PCT/IB2012/055564 patent/WO2013054311A1/en active Application Filing
-
2018
- 2018-04-06 JP JP2018073545A patent/JP6782274B2/ja active Active
- 2018-04-19 US US15/957,694 patent/US11299762B2/en active Active
-
2022
- 2022-03-14 US US17/693,682 patent/US20220195489A1/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090053709A1 (en) * | 2007-02-16 | 2009-02-26 | Drexel University | Enhanced sensitivity of a cantilever sensor via specific bindings |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10947576B2 (en) | 2017-07-25 | 2021-03-16 | Arizona Board Of Regents On Behalf Of Arizona State University | Rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells |
US11198897B2 (en) | 2017-07-25 | 2021-12-14 | Arizona Board Of Regents On Behalf Of Arizona State University | Rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells |
Also Published As
Publication number | Publication date |
---|---|
US20180312898A1 (en) | 2018-11-01 |
EP2766722B1 (en) | 2018-12-26 |
JP6782274B2 (ja) | 2020-11-11 |
WO2013054311A1 (en) | 2013-04-18 |
JP2018136332A (ja) | 2018-08-30 |
US11299762B2 (en) | 2022-04-12 |
TR201901893T4 (tr) | 2019-03-21 |
PL2766722T3 (pl) | 2019-07-31 |
DK2766722T3 (en) | 2019-03-18 |
EP2766722A1 (en) | 2014-08-20 |
US20220195489A1 (en) | 2022-06-23 |
ES2710191T3 (es) | 2019-04-23 |
JP2014528591A (ja) | 2014-10-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20220195489A1 (en) | Nanoscale motion detector | |
Syal et al. | Antimicrobial susceptibility test with plasmonic imaging and tracking of single bacterial motions on nanometer scale | |
Tang et al. | Characterization and analysis of mycobacteria and Gram-negative bacteria and co-culture mixtures by Raman microspectroscopy, FTIR, and atomic force microscopy | |
Gilbert et al. | Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction | |
Sha et al. | Identification of spherical and nonspherical proteins by a solid-state nanopore | |
Yoshikawa et al. | Nanomechanical membrane-type surface stress sensor | |
Marszalek et al. | Stretching single polysaccharides and proteins using atomic force microscopy | |
Pujol-Vila et al. | Nanomechanical sensors as a tool for bacteria detection and antibiotic susceptibility testing | |
Mustazzolu et al. | A rapid unraveling of the activity and antibiotic susceptibility of mycobacteria | |
Kohler et al. | Nanomotion detection based on atomic force microscopy cantilevers | |
Formosa-Dague et al. | Cell biology of microbes and pharmacology of antimicrobial drugs explored by Atomic Force Microscopy | |
Elishakoff et al. | Clamped-free double-walled carbon nanotube-based mass sensor | |
Perret et al. | Real-time mechanical characterization of DNA degradation under therapeutic X-rays and its theoretical modeling | |
Huber et al. | Nanosensors for cancer detection. | |
Maloney et al. | Nanomechanical sensors for single microbial cell growth monitoring | |
EP1342789B1 (en) | Cantilever apparatus and method for detecting microorganisms | |
Mertens et al. | Nanomechanical detection of Escherichia coli infection by bacteriophage T7 using cantilever sensors | |
JP6796561B2 (ja) | 生体試料分析装置、及び方法 | |
NL2024356B1 (en) | 2D material detector for activity monitoring of single living micro-organisms and nano-organisms | |
CN104965069A (zh) | 细胞活性在线检测和药物筛选方法及装置 | |
Tzeng et al. | Adhesin-specific nanomechanical cantilever biosensors for detection of microorganisms | |
Vineetha et al. | Performance analysis of MEMS sensor for the detection of cholera and diarrhea | |
Floto et al. | A rapid unravelling of mycobacterial activity and of their susceptibility to antibiotics | |
Chan et al. | Selective weighing of individual microparticles using a hybrid micromanipulator-nanomechanical resonator system | |
Katole et al. | Design of MEMS Biosensor for Pathogenic Bacterial Disease Detection. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL), S Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:KASAS, SANDOR;LONGO, GIOVANNI;DIETLER, GIOVANNI;AND OTHERS;SIGNING DATES FROM 20140416 TO 20140422;REEL/FRAME:032753/0510 |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- AFTER EXAMINER'S ANSWER OR BOARD OF APPEALS DECISION |