JP2018136332A - ナノスケール運動検出器(Motion Detector) - Google Patents
ナノスケール運動検出器(Motion Detector) Download PDFInfo
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Abstract
【解決手段】少なくとも1つの物体を保持するように適合された可撓性支持体と、前記支持体の変位を測定するためのセンサと、前記物体によって誘起される変動から前記支持体の変動を区別するための処理手段とを備える運動検出器を構成することで、細胞および細菌の単一分子の立体構造変化、生化学反応、薬物標的の相互作用、ならびに内部および外部運動を監視するために使用することができる。
【選択図】図3
Description
薬物親和性の検出
これらの実験は、トポイソメラーゼII(TopoII、図3)、および抗がん剤に対するその相互作用に関する。TopoIIは、DNAと相互作用してそのトポロジーを簡略化し、転写を安全に行うことを可能にする必須酵素である。
生化学反応の検出
前述のように、TopoIIは、DNAと相互作用してそのトポロジーを簡略化する。発明者らの方法を用いてこの反応を記録するために、発明者らは、図6に示すように、AFMカンチレバーの両側にTopoII−スーパーコイルDNA複合体を堆積させた。
抗生物質感受性検出
本方法は、細菌の外部ならびに内部の運動を検出するのに十分なほど感度が高く、抗菌剤の作用を評価するために使用することができる。この実験は、秒から分の範囲の非常に高い時間分解能で抗生物質に対する細菌の感度を探る能力を示す。
1 Boisen et al., Rep. Prog. Phys., 74 (2011) 036101
2 Ilic et al., Appl. Phys. Lett., 77 (2000) 450-452
3 Braun et al., Nature Nanotech., 4 (2009) 179-185
4 Lui et al., Sensors 2008 IEEE, 1464-1467
5 特許 US 7148017B1
6 特許出願 US 2009235746A1
7 特許出願 WO 2011021984A1
8 Fritz et al., Science, 288 (2000) 316-318
9 Berger et al., Science, 276 (1997) 2021-2024
10 Godin et al., Nanotech., 21 (2010) 075501
11 特許 EP 1342789A2
12 特許 WO 0058729A2
13 特許 WO 03023363A2
14 Luckulum et al., Anal. Bioanal. Chem., 384 (2006) 667-682
15 Jensen et al., Cancer Res., 51 (1991): 5093-5099
16 Mckendry et al., PNAS, 99 (2002) 9783-9788
17 Gupta et al., J. Vac. Sci. Technol. B, 22 (2004) 2785-2791
18 Campbell et al., J. Bios. Bioel., 21 (2005) 462-473
19 Djuric et al., J. Microel. Eng., 84 (2007) 1639-1642
20 特許出願 US 2006121502A1
21 特許出願 US 2003045019A1
22 特許出願 WO 2004038762A2
23 特許出願 WO 03081204A2
24 特許出願 US 2008136291A1
2 レーザ・ビーム
3 鏡
4 光検出器
5 光ファイバ
6 動いている標本
7 TopoII
8 DNA分子
9 細菌
Claims (17)
- 少なくとも1つの物体(6〜9)を保持するように適合された可撓性支持体(1、5)と、前記支持体(1)の変位を測定するためのセンサ(4)と、前記物体(6〜9)によって誘起される変動から前記支持体(1)の変動を区別するための処理手段とを備える運動検出器。
- 前記処理手段は、前記変位の分散を計算するように適合される、請求項1に記載の運動検出器。
- 前記支持体はカンチレバー(1)を備える、請求項1または2に記載の運動検出器。
- 前記センサは、前記カンチレバーの一部に固定され、または前記カンチレバーの一部を形成する、請求項3に記載の運動検出器。
- センサは圧電材料を備える、請求項4に記載の運動検出器。
- 前記センサ(4)は光センサである、請求項1〜4のいずれか1項に記載の運動検出器。
- 前記光センサは光検出器(4)である、請求項6に記載の運動検出器。
- 分析チャンバをさらに備える、請求項1〜7のいずれか1項に記載の運動検出器。
- 前記分析チャンバは、前記チャンバ内で物体(複数可)(6〜9)に対して異なる流体環境を提供するように適合された入口および出口手段を備える、請求項8に記載の運動検出器。
- 前記支持体(1)上に位置する少なくとも1つの物体(6〜9)を分析するための請求項1〜9のいずれか1項に記載の運動検出器の使用。
- 前記物体(6〜9)は生物学的物体である、請求項10に記載の使用。
- 前記生体は細菌(6、9)である、請求項11に記載の使用。
- MICおよび/またはMBCの定量的な推定のための請求項10〜12のいずれか1項に記載の使用。
- 可撓性支持体(1)上に保持された少なくとも1つの物体を分析する方法であって;前記支持体(1)の変位を測定する工程と、前記物体(6〜9)によって誘起される変動から前記支持体(1)の変動を区別するための処理工程とを含む上記方法。
- 少なくとも1つの物体(6〜9)の動きまたはダイナミクスを検出する、請求項14に記載の方法。
- 前記処理工程は、前記変位の分散を計算する工程を含む、請求項14または15に記載の方法。
- 前記支持体(1、5)に対する前記物体(6〜9)の粘着を容易にするために物体(6〜9)に適用される前処理を含む、請求項14〜16のいずれか1項に記載の方法。
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US (3) | US20140287403A1 (ja) |
EP (1) | EP2766722B1 (ja) |
JP (2) | JP2014528591A (ja) |
DK (1) | DK2766722T3 (ja) |
ES (1) | ES2710191T3 (ja) |
PL (1) | PL2766722T3 (ja) |
TR (1) | TR201901893T4 (ja) |
WO (1) | WO2013054311A1 (ja) |
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WO2014145765A1 (en) | 2013-03-15 | 2014-09-18 | Ancera, Inc. | Systems and methods for bead-based assays in ferrofluids |
US11285490B2 (en) | 2015-06-26 | 2022-03-29 | Ancera, Llc | Background defocusing and clearing in ferrofluid-based capture assays |
US10947576B2 (en) | 2017-07-25 | 2021-03-16 | Arizona Board Of Regents On Behalf Of Arizona State University | Rapid antibiotic susceptibility testing by tracking sub-micron scale motion of single bacterial cells |
NL2024356B1 (en) | 2019-12-02 | 2021-08-31 | Univ Delft Tech | 2D material detector for activity monitoring of single living micro-organisms and nano-organisms |
CN114829620A (zh) | 2019-12-23 | 2022-07-29 | 雷希斯特尔股份公司 | 生物物体和非生物物体例如细菌细胞向表面例如悬臂的附着 |
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JP2014528591A (ja) | 2014-10-27 |
US11299762B2 (en) | 2022-04-12 |
WO2013054311A1 (en) | 2013-04-18 |
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ES2710191T3 (es) | 2019-04-23 |
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