US20050084482A1

US20050084482A1 - Use of lactobacillus salivarius

Info

Publication number: US20050084482A1
Application number: US10/804,223
Authority: US
Inventors: John Collins; Gerald O'Sullivan; Liam O'Mahony; Fergus Shanahan
Original assignee: Enterprise Ireland
Current assignee: University College Cork; Enterprise Ireland
Priority date: 1999-01-15
Filing date: 2004-03-19
Publication date: 2005-04-21
Also published as: CN1342196A; US20030215467A1; CA2359334C; IL144184A0; US20060002908A1; EP1143985B1; CA2360243A1; JP4706016B2; HK1044964A1; AU779405B2; CN1245994C; RU2001119046A; NO327792B1; US20030170217A1; US20070141039A1; ID29150A; DE60035670T2; US20080311080A1; BRPI0007481B8; AU3071600A

Abstract

Lactobacillus salivarius is useful in the prophylaxis or treatment of undesirable inflammatory activity, especially gastrointestinal inflammatory activity such as inflammatory bowel disease or irritable bowel syndrome. The inflammatory activity may also be due to cancer. The Lactobacillus salivarius is of human origin isolated from resected and washed human gastrointestinal tract. One such strain is UCC 118 described in WO-A-9835014.

Description

The invention relates to the use of strains of Lactobacillus salivarius.
The defense mechanisms to protect the human gastrointestinal tract from colonization by intestinal bacteria are highly complex and involve both immunological and non-immunological aspects (V. J. McCracken and H. R Gaskins, ‘Probiotics a critical review’, Horizon Scientific Press, UK, 1999, p 278.). Innate deference mechanisms include the low pH of the stomach, bile salts, peristalsis, much layers and anti-microbial compounds such as lysozyme (D. C. Savage, ‘Microbial Ecology of the Gut’, Academic Press, London, 1997, p.278.). Immunological mechanisms include specialised lymphoid aggregates, underlying M cells, called peyers patches which are distributed throughout the small intestine and colon (M. F. Kagnoff. Gastrocaterol. 1993, 105, 1275). Luminal antigens presented at these sites result in stimulation of appropriate T and B cell subsets with establishment of cytoline networks and secretion of antibodies into the gastrointestinal tract (M. R. Neutra and J- P Kraehenbuhl, ‘Essentials of mucosal immunology’, Academic Press, San Diego, 1996, p.29., M. E. Lamm. Ann. Rev. Mirobiol. 1997, 51, 311). In addition, antigen presentation may occur via epithelial cells to intraepithelial lymphocytes and to the underlying lamina propria immune cells (S. Raychaudhur et al. Nat Biotecinol., 1998, 16, 1025). Therefore, the host invests substantially in immunological defense of the gastrointestinal tract. However, as the gastrointestinal raucous is the largest surface at which the host interacts with the external environment, specific control mechanisms must be in place to regulate immune responsiveness to the 100 tons of food which is handled by the gastrointestinal tract over an average lifetime (F. Shanahan, ‘Physiology of the gastrointestinal tract’, Raven Press, 1994, p.643.). Furthermore, the gut is colonised by over 500 species of bacteria numbering 10¹¹-10¹²/g in the colon. Thus, these control mechanisms must be capable of distinguishing non-pathogenic adherent bacteria from invasive pathogens which would cause significant damage to the host. In fact, the intestinal flora contributes to defense of the host by competing with newly ingested potentially pathogenic microorganisms.

Consumption of non-pathogenic, or probiotic, bacteria has resulted in enhancement of immune parameters in healthy volunteers. Examples of these immune modulatory effects are given in Table 1.

TABLE 1


Immune Enhancing Effects Following Oral Consumption of Probiotic
Bacteria.

	Observed Effect	Reference

	Increased Macrophage Phagocytosis	10
	Increased Natural Killer Cell Activity	11
	Increased IFNγ serum levels	12
	Increased B cell and NK cell numbers	12
	Promotion of IgA responses	11, 13-15
	Increased DTH responses	16

Bacteria present in the human gastrointestinal tract can promote inflammation Aberrant immune responses to the indigenous ricroflora have been implicated in certain disease states, such as inflammatory bowel disease (Brandzeag P. et al. Springer Semin. Immunopathol., 1997, 18, 555). Antigens associated with the normal flora usually lead to immunological tolerance and failure to achieve this tolerance is a major mechanism of mucosal inflammation (Stallmach A. et al., Immunol. Today, 1998, 19, 438). Evidence for this breakdown in tolerance includes an increase in antibody levels directed against the gut flora in patients with IBD.
WO-A-98/35014 describes strains of Lactobacillus salivarius isolated from resected and washed human gastrointestinal tract which inhibits a broad range of Gram positive and Gram negative microorganisms and which secretes a product having anti-microbial activity into a cell-free supernatant.

STATEMENTS OF INVENTION

The immune system is designed to defend host tissue and destroy invading pathogens. Upon recognition of the presence of a bacterial cell, cells of the immune system become activated and eliminate the bacterial threat. The production of inflammatory mediators promote cellular activation and pathogen destruction.
Surprisingly, we have found that strains of Lactobacillus salivarius elicit an anti-inflammatory effect in vitro and an vivo. We have found that the immune perception of Lactobacillus salivarius results in the suppression of inflammatory activity. The deliberate consumption in large numbers of Lactobacillus salivarius results in the suppression of inflammatory activity. The invention is therefore of major potential therapeutic value in the prophylaxis or treatment of undesirable inflammatory responses, such as inflammatory bowel disease.
Lactobacillus salivarius is a commensal microorganism originally isolated from the microbial flora within the human gastrointestinal tract. The immune system within the gastrointestinal tract cannot have a pronounced reaction to members of this flora as the resulting inflammatory activity would also destroy host cells and tissue function. Therefore, some mechanism(s) exist whereby the immune system can recognise commensal non-pathogenic members of the gastrointestinal flora as being different to pathogenic organisms. This ensures that damage to host tissues is restricted and a defensive barrier is still maintained.
According to the invention there is provided use of a strain of Lactobacillus salivarius in the prophylaxis and/or treatment of undesirable inflammatory activity.
The invention the undesirable inflammatory activity may be undesirable gastrointestinal inflammatory activity such as inflammatory bowel disease, eg. Crohns disease, ulcerative colitis, irritable bowel syndrome, pouchitis or post infection colitis.
The gastrointes inflammatory activity may also be diarrhoeal disease. The diarrhoeal disease may be associated by Clostridium difficile or be associated with Rotoviius. The diarrhoeal disease may also be post infective diarrhoeal disease.
The inflammatory activity may be due to gastrointestinal cancer or systemic inflammatory disease such as rheumatoid arthritis.
In another instance the undesirable inflammatory activity may be due to an autoimmune disorder.
In yet another instance the undesirable inflammatory activity may be due to cancer.
In one embodiment the invention provides use of a strain of Lactobacillus salivarius in the prophylaxis of cancer.
In another embodiment the invention provides use of a strain of Lactobacillus salivarius wherein the Lactobacillus salivarius is contained in a formulation.
Preferably the formulation includes another probiotic material. Alternatively or additionally the formulation includes a prebiotic material.
Ideally the formulation includes an ingestive carrier. The ingestable carrier may be a pharmaceutically acceptable carrier such as a tablet, capsule or powder.
Preferably the ingestable carrier is a protein and/or a peptide, in particular proteins and/or peptides that are rich in glutamine/glutamate; a lipid; a carbohydrate; a vitamin; mineral and/or trace element.
Most preferably the ingestable carrier is a food product such as acidified milk, yoghurt, frozen yoghurt, milk powder, milk concentrate, cheese spreads, dressings or beverages.
In one embodiment the Lactobacillus salivarius is present in the formulation at more than 10⁶cfu per gram of delivery system.
In another embodiment the formulation includes an adjuvant. The formulation may include a bacterial component. The formulation may alternatively or additionally include a drug entity. The formulation may also include a biological compound.
In one embodiment the invention provides use of a strain of Lactobacillus salivarius wherein the strain or formulation is for administration to animals. Preferably the animal is a mammal, most preferably a human.
In another embodiment the invention provides use of a strain of Lactobacillus salivarius wherein the Lactobacillus salivarius effects changes in an immunological marker when introduced into a system comprising cells which interact with the immune system and cells of the immune system.
Preferably the cells which interact with the immune system are epithelial cells. Most preferably the immunological marker is a cytokine especially TNFα.
Preferably the cells which interact with the immune system and the immune system cells are of matched origin.
In one embodiment the cells which interact with the immune system are of gastrointestinal, respiratory or genitourinary origin.
In another embodiment the cells of the immune system are of gastrointestinal, respiratory or genitourinary origin.
In a further embodiment the invention provides use of a strain of Lactobacillus salivarius wherein the Lactobacillus salivarius strain is Lactobacallius salivarius subspecies salivarius. Preferably the Lactobacallius salivarius is of human origin, most preferably from resected and washed human gastrointestinal tract.
Preferably the Lactobacillus salivarius inhibits a broad range of Gram positive and Gram negative micro-organisms. Most preferably it secretes a product having anti-microbial activity into a cell-free supernatant, said activity being produced only by growing cells and being destroyed by proteinase K and pronase E.
An especially preferred strain of Lactobacillus salivarius is Lactobacallius salivarius strain UCC188 or mutant or variant thereof.
A deposit of Lactobacillus salivarius strain UCC118 was made at the NCIB on Nov. 27, 1996 and accorded the accession number NCMB 40829. The strain of Lactobacillus salivarius is described in WOA-98/35014.
The Lactobacillus salivarius may be a genetically modified mutant or it may be a naturally occurring van ant of Lactobacillus salivarius.
Preferably the Lactobacillus salivarius is in the form of viable cells. Alternatively the Lactobacillus salivarius may be in the form of non-viable cells.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a graph of C. perfingens levels in the mice consuming UCC118 compared to a placebo group (p<0.05). Results are plotted as the mean log values±standard error for each of the groups
FIG. 2 is a bar chart of inflammatory scores for mice consuming UCC118 in comparison to control mince. Results are shown as the mean±standard error for each of the groups.
FIG. 3 is a graph of TNFα levels over six weeks that patients consume UCC118. Results are plotted as the mean pg/ml TNFα level for each time point (n=22).
FIG. 4 is a graph of CDAI scores for patients consuming UCC118 over the course of probiotic feeding. CDAI scores decreased from an average of 180 to 160.
FIG. 5 is a graph of cytokine production in vivo following exposure to UCC118, Results are expressed as pg/ml.
FIG. 6 is bar chart of extracellular TNFα, IL-1 RA, IL-6, sIL-6R, and IFNα levels following exposure to Lactobacillus salivarius UCC118.
FIG. 7 is a gene array with specific gene sequences for 268 cytokines and related molecules to examine the immune response to UCC118. The bottom panel illustrates the control culture while the top panel illustrates cytokine gene expression by PBMCs following exposure to UCC118; and
FIG. 8 is a bar chart of TNFα levels in the presence of various bacterial strains.

DETAILED DESCRIPTION

We have developed criteria for in vitro selection of probiotic bacteria that reflect certain in vivo effects on their host, such as modulation of the GIT microflora and modulation of the mucosal immune response resulting in the production of secretory antibodies specific to the consumed strain. We have found that Lactobacillus salivarius subsp. salivarius UCC118 not only survives passage through the gastrointestinal tract and adheres to human intestinal cell lines but also, surprisingly has anti-inflammatory effects.
The general use of probiotic bacteria is in the form of viable cells. However, it can also be extended to non-viable cells such as killed cultures or -compositions containing beneficial factors expressed by the probiotic bacteria. This could include thermally killed micro-organisms or micro-organisms killed by exposure to altered pH or subjection to pressure. With non-viable cells product preparation is simpler, cells may be incorporated easily into pharmaceuticals and storage requirements are much less limited than viable cells. Lactobacillus casei YIT 9018 offers an example of the effective use of heat killed cells as a method for the treatment and/or prevention of tumour growth as described in US Patent -No. U.S. Pat. No. 4,347,240.
It is unknown whether intact bacteria are required to exert an anti-inflammatory effect or if individual active components of the invention can be utllised alone. Proinflammatory components of certain bacterial strains have been identified. The proinflammatory effects of gram-negative bacteria are mediated by lipopolysaccharide (LPS). LPS alone induces a proinflammatory network, partially due to LPS binding to the CD14 receptor on monocytes. It is assumed that components of probiocic bacteria possess anti-inflammatory activity, due to the effects of the whole cell. Upon isolation of these components, pharmaceutical grade manipulation is anticipated.
The invention will be more clearly understood from the following examples.

EXAMPLE 1

Detailed Description of the In Vivo Demonstration of the Anti-Inflammatory Effects of Lactobacillus salivarius Especially Subspecies salivarius UCC118.

Murine Model of Gastrointestinal Inflammation
Aberrant immune responses to the indigenous microflora have been implicated in certain disease states, such as inflammatory bowel disease (Brandzeag P., et al. Springer Semin. Immunopathol., 1991, 18, 555). Antigens associated with the normal flora usually lead to immunological tolerance and failure to achieve this tolerance is a major mechanism of mucosal inflammation (Stallmach A., et al. Immunol. Today, 1998, 19, 438). Evidence for this breakdown in tolerance includes an increase in antibody levels directed against the gut flora in patients with IBD. In addition, certain mouse models predisposed to inflammatory lesions in the gastrointestinal tract remain disease free when housed in germ free conditions or when treated with antibiotics (Kuhn R., et al. Cell, 1993, 75, 263; Panwala C. M., et al. J. Immunol., 1998, 161, 5733).
C57BL/6 Interleukin-10 knockout mice are predisposed to developing enterocolitis in the presence of an enteric bacterial flora. When maintained in germ free conditions, IL-10 knock out mice do not develop the disease (Kuhn R., et al. Cell, 1993, 75, 263). Since the pathogenesis of this disease has been linked with the enteric flora, elimination of specific components of this flora may have a beneficial effect on the severity of this disease.
Lactobacillus salivarius subsp. salivarius UCC118 is a probiotic bacteria, which was isolated from a healthy human ileum. It is suited to gastrointestinal colonization as it fulfills many criteria set down for the selection of probiotic strains. These include traits such as bile tolerance, acid resistance and in vitro adherence to human colonic cell lines. Feeding trials in healthy humans have been conducted and considerable modification of the gastrointestinal flora was noted. In addition, UCC118 was perceived by the mucosal immune system resulting in the production and secretion of IgA specific to UCC118.
Thus, UCC118 survives passage through the gastrointestinal tract, modulates the gut flora and is perceived by the mucosal immune system. The influence of this probiotic bacteria in modulating inflammatory responses within the gastrointestinal tract was examined using a murine model of enterocolitis. In addition, we examined the role of Lactobacillus salivarius subsp. salivarius UCC118 in reducing the rate of neoplastic change within the gastrointestinal tract.
Twenty IL-10 KO mice were studied (ten consumed probiotic organisms in milk and 10 consumed unmodified milk) for 16 weeks. Fecal microbial analysis was performed weekly to enumerate excretion of lactobacilli, Clostridium perfringens, bacteroides, coliforms, bifidobacteria and enterococci. At sacrifice, small and large bowel were microbiologically and histologically assessed.
Fecal coliform and enterococci levels were significantly reduced in test animals compared to the controls. At sacrifice, a significant reduction in C. perfringens numbers was observed in the test mice (FIG. 1). There were no fatalities in the test group compared to two deaths from fulminant colitis in the control group. Only one test mouse developed colonic adenocarcinoma compared to five in the control group. Test animal mucosal inflammation consistently scored lower than that of the control mice (FIG. 2). The reduction in tumour incidence following consumption of UCC118 may be related to the reduced level of inflammation within the gastrointestinal tract or may be due to elimination of pro-carcinogenic members of the gastrointestinal flora (Rumney C. J., et al. Carcinogenesis, 1993, 14, 79; Rowland I. R. (1995). In: Gibson G. R. (ed). Human colonic bacteria: role in nutrition. physiology and pathology, pp 155-174. Boca Raton CRC Press: Darveau D. Nat. Biotech., 1999, 17, 19).
In conclusion, consumption of Lactobacillus salivarius UCC118 results in a significant modulation of the gut flora and an improvement in mortality rate, cancer incidence and disease score.

EXAMPLE 2

Human Trial with UCC118 in Patients with Active Crohn's Disease.

Inflammatory bowel disease (IBD) encompasses a number of inflammatory disorders of the gastrointestinal tract, including both Crohn's disease and ulcerative colitis.
Patients suffering from active Crohn's disease have been treated with UCC118. Briefly, UCC118 was consumed in a fermented milk product for 6 weeks by 22 patients. Microbiological and immunological determinations were made at week 0, week 1, week 3 and week 6. This was not a placebo-controlled trial.
A number of systemic cytoline levels were measured over the course of feeding. In particular, tumour necrosis factor α (TNFα), a proinflammatory cytokine that has been implicated in the pathogenesis of many inflammatory disease states, including inflammatory bowel disease. Current therapies for inflammatory bowel disease specifically aim to reduce TNFα levels (Present. D. H., et al. New Eng. J. Med., 1999, 340, 1398). In this trial, systemic TNFα levels were reduced following consumption of UCC118 (FIG. 3).
In addition, patients were assessed regarding their Crohn's Disease Activity Index (CDAI) over the six week trial period. This index assesses the general health and well being of each patient (FIG. 4). Overall, the disease activity index improved slightly for the majority of individuals in the trial. These are patients with moderately active disease and their CDAI scores would be expected to increase. However, following treatment with UCC118, CDAI scores did not increase and in fact they improved from a mean of 180 to 160.

EXAMPLE 3

Detailed Description of the In Vitro Demonstration of the Mechanisms Underlying the Anti-Inflammatory Effects of Lactobacillus salivarius Especially Subspecies salivarius UCC118.

A number of methodologies have been utilised for these studies including ELISAs (extracellular protein determination), flow cytometry (intracellular protein determination) and cDNA expression arrays (mRNA expression). In particular, examination of the expression of tumour necrosis factor a has been targeted, due to its clinical importance, and suppression of the production of this cytoline, following exposure to UCC118, has been noted using all three methodologies.
Using a transwell assay system, with epithelial cells and peripheral blood mononuclear cells, extracellular cytoline levels were measured by ELISAs. Following to-incubation with UCC118, the amount of TNFα produced, was significantly reduced compared to control cultures. Furthermore, IL-IRA and IFNγ levels dropped while IL6 and soluble IL-6 receptor levels increased (FIG. 5). Intracellular staining for TNFα confirmed the ELISA result as TNFα levels were lowering the UCC118 stimulated sample compared to controls.
FIG. 6 demonstrates the tricellular signalling that occurs. To-incubation of PBMCs and Lactobacillus salivarius strain UCC118 results in the stimulation of TNFα production. However, to-incubation of PBMCs, Lactobacillus salivarius strain UCC118 and epithelial cells (CaCo-2 cells) results in a significant inhibition of TNFα production. Thus, a significantly different pattern of signalling is present in the tricellular model compared to bacteria and PBMCs alone.
Gene arrays measure the quantity of mRNA in a population of cells. We stimulated peripheral blood mononuclear ceUs with UCC118 for 24 hours and we examined the effect on cyeoline gene expression (FIG. 7). Considerable modification of cytokine gene expression was noted. For example, genes encoding the proinflammatory cytokines IL-1 P and TNFα were turned off while genes encoding Th2 type cytokines, such as IL-6, were enhanced.
In vitro models have demonstrated that UCC118 is capable of inducing Th2 type cytoldnes (i.e. IL-6 and IL-6 soluble receptor) while suppressing the production of inflammatory cytokines such as TNFα and IL-1β. Thus, these results suggest that consumption of UCC118 would be of benefit to patients suffering from inflammatory diseases, such as IBD.

EXAMPLE 4

Test for Anti-Inflammatory Bacterial Strains

A number of lactic acid bacteria, which have been isolated from the human gastrointestinal tract, were examined in this novel assay system for anti-inflammatory effect. All bacterial strains were taken from −20° C. glycerol stocks and incubated anaerobically overnight in MRS broth and washed in antibiotic containing medium. Epithelial cell monolayers were grown for 6 weeks prior to the addition of PBMCs and bacterial cells.
The results of these stimulations can be observed in FIG. 8. Relative to control cultures, two bacterial strains suppressed TNFα production. The two strains Lactobacillus salivarius strain UCC118, which suppressed production of TNFα, is the subject of WO-A-9835014. The Bifdobacterium longum infantis strain UCC 35624 is the subject of a PCT Application filed concurrently with the present application.
Inflammation
Inflammation is the term used to describe the local accumulation of fluid, plasma proteins and white blood cells at a site that has sustained physical damage, infection or where there is an ongoing immune response. Control of the inflammatory response is exerted on a number of levels (for review see Henderson B., and Wilson M. 1998. In “Bacteria-Cytokine interactions in health and disease. Portland Press, 79-130). The controlling factors include cytokines, hormones (e.g. hydrocortisone), prostaglandins, reactive intermediates and leukotrienes. Cytokines are low molecular weight biologically active proteins that are involved in the generation and control of immunological and inflammatory responses, while also regulating development, tissue repair and haematopoiesis. They provide a means of communication between leukocytes themselves and also with other cell types. Most cytolines are pleiotrophic and express multiple biologically overlapping activities. Cytokine cascades and networks control the inflammatory response rather than the action of a particular cytokine on a particular cell type (Aral K I, et al., Annu Rev Biochem 1990;59:783-836). Warning of the inflammatory response results in lower concentrations of the appropriate activating signals and other inflammatory mediators leading to the cessation of the inflammatory response. TNFα is a pivotal proinflammatory cytolone as it initiates a cascade of cytokines and biological effects resulting in the inflammatory state. Therefore, agents which inhibit TNFα are currently being used for the treatment of inflammatory diseases, e.g. inliximab.
Pro-inflammatory cytokines are thought to play a major role in the pathogenesis of many inflammatory diseases, including inflammatory bowel disease (IBD). Current therapies for treating IBD are aimed at reducing the levels of these pro-inflammatory cytokines, including IL-8 and TNFα. Such therapies may also play a significant role in the treatmentc of systemic inflammatory diseases such as rheumatoid arthritis.
In view of the anti-inflammatory properties of Lactobacillus salivarius that we have discovered these strains may have potential application in the treatment of a range of inflammatory diseases, particularly if used in combination with other anti-inflammatory therapies, such as non-steroid anti-inflammatory drugs (NSAIDs) or Infliximab.
Diarrhoeal Disease.
The barrier function of the intestinal epithelium can be diminished during nervous (acetylcholine) and immune (histamine) mediated secretion. Certain bacterial toxins may also induce Ca2+ and PKC dependent secretion and thereby can disturb the epithelial barrier (Ganguly N K and Kaur T. Indian J Med Res 1996;104:28-37, Groot J A. Vet Q 1998;20(S3):45-9). Several studies have examined the prevention and treatment of diarrhoeal using probiotic bacteria. Prospective studies have demonstrated the efficacy of lactic acid bacteria administration for both prophylactic and therapeutic use against diarrhoea in pre-mature infants, new borns, children (Isolauri E, et al., Dig Dis Sci 1994 December;39(12):2595-600) and in the treatment of antibiotic related diarrhoea (Siitonen S, et al., Ann Med 1990 February;22(1):57-9) and traveller's diarrhoea (Oksanen P J, et al., Ann Med 1990 February;22(1):53-6).
In view of the anti-inflammatory effect we have discovered Lactobacillus salivarius may also produce an anti-diarrhoeal effect, possibly medicated via cAMP modulation. Cyclic AMP-dependent Cl-secretion is the major secretory pathway in the human intestine (Brzuszczak I M, et al., J. Gastroenterol. Hepatol. 1996;11(9):804-10). The anti-diarrhoeal effect may not be restricted just to diarrhoea resulting from gastrointestinal inflammation, but can be applied to the general treatment of diarrhoeal disease.
Autoimmune Disease
The immune system has a large repertoire of specificities expressed by B and T cells. Some of these specificities will be directed to self-components. Self-recognition is normally controlled by clonal deletion and inactivation of self-reactive lymphocytes. However, there is a constant background of autoimmunity with antibodies to many proteins being found in serum. A breakdown in the self-nonself recognition system results in autoimmunity. When autoimmune disease does occur, the resulting immune response damages the tissue bearing the offending antigen. Immune complex deposition, type II hypersensitivity and cell-mediated reactions are the most important mechanisms by which immunopathological damage occurs. Examples of autoimmune diseases include, but are not limited to, systemic lupus erythematosus, rheumatoid arthritis, insulin dependent diabetes melitus, myasthenia gravis and pernicious anaenua. We have found that Lactobacillus salivarius is an immunomodulatory bacterium. Thus, consumption either as a single component or in combination with other bacteria by patients suffering from autoimmune disease may restrict organ damage and help restore normal body homeostasis.
Inflammation and Cancer
The production of multifunctional cytolines across a wide spectrum of tumour types suggests that significant inflammatory responses are ongoing in patients with cancer. It is currently unclear what protective effect this response has against the growth and development of tumour cells in vivo. However, these inflammatory responses could adversely affect the tumour bearing host. Complex cytokine interactions are involved in the regulation of cytokine production and cell proliferation within tumour and normal tissues (McGee D W, et al., Immunology 1995 September;86(l):6-11, Wu S, et al.,. Gynecol Oncol 1994 April;53(.1):59-63). It has long been recognized that weight loss (cachexia) is the single most common cause of death in patience with cancer (Inagaki J. et al. Cancer 1974 February;33(2):568-73) and initial malnutrition indicates a poor prognosis (Van Eys J. Nutr Rev 1982 December;40(12):353-9). For a tumour to grow and spread it must induce the formation of new blood vessels and degrade the extracellular matrix. The inflammatory response may have significant roles to play in the above mechanisms, thus contributing to the decline of the host and progression of the tumour. Due to the anti-inflammatory properties of Lactobacillus salivarius these bacterial strains they may reduce the rate of malignant cell transformation. Furthermore, intestinal bacteria can produce, from dietary compounds, substances with genotoxic, carcinogenic and tumour-promoting activity and gut bacteria can activate pro-carcinogens to DNA reactive agents (Rowland I. R. (1995). Toxicology of the colon: role of the intestinal microflora. In: Gibson G. R. (ed). Human colonic bacteria: role in nutrition, physiology and pathology, pp 155-174. Boca Raton CRC Press). In general, species of Lactobacillus have low activities of xenobiotic metabolising enzymes compared to other populations within the gut such as bacteroides, eubacteria and clostridia (Saito Y., et al., Microb. Ecol. Health Dis., 1992;5, 105-110). Therefore, increasing the number of Lactobacillus bacteria in the gut could beneficially modify the levels of these enzymes.
Prebiotics
The introduction of probiotic organisms is accomplished by the ingestion of the microorganism in a suitable carrier. It would be advantageous to provide a medium that would promote the growth of these probiotic strains in the large bowel. The addition of one or more oligosaccharides, polysaccharides, or other prebiotics enhances the growth of lactic acid bacteria in the gastrointestinal tract (Gibson, G R. Br. J. Nutr. 1998;80 (4):S209-12). Prebiotics refers to any non-viable food component that is specifically fermented in the colon by indigenous bacteria thought to be of positive value, e.g. bifidobacteria, lactobacilli. Types of prebiotics may include those which contain fructose, xylose, soya, galactose. glucose and mannose. The combined administration of a probiotic strain with one or more prebiotic compounds, may enhance the growth of the administered probiotic in vivo resulting in a more pronounced health benefit, and is termed symbiotic.
Other Active Ingredients
It will be appreciated that the Lactobacillus salivarius may be administered prophylactically or as a method of treatment either on its own or with other probiotic and/or prebiotic materials as described above. In addition, the bacteria may be used as part of a prophylactic or treatment regime using other active materials such as those used for treating inflammation or other disorders, especially those of the gastrointestinal tract. Such combinations may be administered in a single formulation or as separate formulations administered at the same or different times and using the same or different routes of administration.
The invention is not limited to the embodiments hereinbefore described which may be varied in detail.

Claims

1-51. (canceled)

52. A method of treating or preventing inflammation or an inflammatory disease in a subject which comprises administering to the subject a preparation comprising a Lactobacillus salivarius strain of Lactobacillus salivarius wherein the Lactobacillus salivarius strain is isolated from resected and washed human gastrointestinal tract.

53. The method of claim 52 wherein the inflammation is gastrointestinal inflammation.

54. The method of claim 52, wherein the inflammatory disease is selected from any one or more of inflammatory bowel disease, Crohn's disease, ulcerative colitis, irritable bowel syndrome, pouchitis, post infection colitis, diarrhoeal disease, and a systemic inflammatory disease.

55. The method of claim 54, wherein the diarrhoeal disease is associated with Clostridium difficile.

56. The method of claim 54, wherein the diarrhoeal disease is associated with Rotovirus.

57. The method of claim 54, wherein the diarrhoeal disease is post infective diarrhoeal disease.

58. The method of claim 53, wherein the inflammation is due to a cancer or an autoimmune disorder.

59. The method of claim 52, wherein the systemic inflammatory disease is rheumatoid arthritis.

60. The method of claim 58, wherein the cancer is gastrointestinal cancer.

61. The method of claim 52, which further comprises administering to the subject another probiotic material.

62. The method of claim 52, which further comprises administering to the subject a prebiotic material.

63. The method of claim 52, wherein the preparation is a formulation comprising an ingestible carrier.

64. The method of claim 63, wherein the ingestible carrier is a pharmaceutically acceptable carrier.

65. The method of claim 64, wherein the formulation is a pharmaceutical formulation in the form of a tablet, a capsule, or a powder.

66. The method of claim 63, wherein the ingestible carrier comprises a protein, a polypeptide, a peptide, or a combination thereof.

67. The method of claim 66, wherein the protein, the polypeptide, or the peptide is rich in glutamine, glutamate, or both.

68. The method of claim 63, wherein the ingestible carrier comprises a lipid, a carbohydrate, a vitamin, a mineral, a trace element, or a combination thereof.

69. The method of claim 63, wherein the ingestible carrier is a food product.

70. The method of claim 69, wherein the food product is selected from any one or more of acidified milk, yoghurt, a frozen yoghurt, a milk powder, a milk concentrate, a cheese spread, a dressing, and a beverage.

71. The method of claim 52, wherein the Lactobacillus salivarius strain is present at more than 10⁶cfu per gram of preparation.

72. The method of claim 52, wherein the preparation comprises an adjuvant, bacterial components, a drug, a biological compound, or a combination thereof.

73. The method of claim 52, wherein the subject is a mammal.

74. The method of claim 73, wherein the mammal is human.

75. The method of claim 52, wherein the Lactobacillus salivarius strain is in the form of viable cells.

76. The method of claim 52, wherein the Lactobacillus salivarius strain is in the form of non-viable cells.

77. The method of claim 52, wherein the Lactobacillus salivarius strain inhibits a broad range of Gram positive and Gram negative microorganisms.

78. The method of claim 52, wherein the Lactobacillus salivarius strain secretes a product having anti-microbial activity into a cell-free supernatant, said activity being produced only by growing cells and being destroyed by proteinase K, pronase E, or both.

79. The method of claim 52, wherein the Lactobacillus salivarius strain is Lactobacillus salivarius subspecies salivarius.

80. The method of claim 52, wherein the Lactobacillus salivarius strain is UCC118 [NCIMB 40829] or a mutant or variant thereof.

81. The method of claim 52, wherein the Lactobacillus salivarius strain is a genetically modified mutant strain.

82. The method of claim 52, wherein the Lactobacillus salivarius strain is a naturally occurring variant of Lactobacillus salivarius.

83. A method of treating or preventing inflammation or an inflammatory disease in a subject comprising administering to the subject a preparation comprising a Lactobacillus salivarius strain of Lactobacillus salivarius wherein the Lactobacillus salivarius is characterised by changing an immunological marker when introduced into a system comprising cells which interact with the immune system and cells of the subject.

84. The method claim 83 wherein the strain of Lactobacillus salivarius is isolated from resected and washed human gastrointestinal tract.

85. The method of claim 83 wherein the cells which interact with the immune system are epithelial cells.

86. The method of claim 83, wherein the immunological marker is a cytokine.

87. The method of claim 86, wherein the cykotine is TNFα.

88. The method of claim 83, wherein the cells which interact with the immune system and the immune system cells are of matched origin.

89. The method of claim 83, wherein the cells which interact with the immune system are of gastrointestinal, respiratory, or genitourinary origin.

90. The method of claim 83, wherein the cells of the immune system are of gastrointestinal, respiratory, or genitourinary origin.

91. A method of treating or preventing inflammation or an inflammatory disease in a subject which comprises administering to the subject a preparation comprising a strain of Lactobacillus salivarius UCC118 [NCIMB 40829] or a mutant or variant thereof.

92. The method of claim 91, wherein the Lactobacillus salivarius strain is a genetically modified mutant strain.

93. The method of claim 91, wherein the Lactobacillus salivarius strain is a naturally occurring variant of Lactobacillus salivarius.

94. The method of claim 91, wherein the Lactobacillus salivarius strain is in the form of viable cells.

95. The method of claim 91, wherein the Lactobacillus salivarius strain is in the form of non-viable cells.

96. A method of treating or preventing cancer in a subject which comprises administering to the subject a Lactobacillus salivarius strain of Lactobacillus salivarius or a mutant or variant thereof.