UA99594C2 - Epsps mutants - Google Patents

Epsps mutants

Info

Publication number
UA99594C2
UA99594C2 UAA200810303A UAA200810303A UA99594C2 UA 99594 C2 UA99594 C2 UA 99594C2 UA A200810303 A UAA200810303 A UA A200810303A UA A200810303 A UAA200810303 A UA A200810303A UA 99594 C2 UA99594 C2 UA 99594C2
Authority
UA
Ukraine
Prior art keywords
epsps
phosphonomethylglytin
herbicide
amino acid
production
Prior art date
Application number
UAA200810303A
Other languages
English (en)
Ukrainian (uk)
Inventor
Грег Ф.В. Гоукал
Original Assignee
Сайбас Юроп Б.В.
Инсима Ипко Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Сайбас Юроп Б.В., Инсима Ипко Б.В. filed Critical Сайбас Юроп Б.В.
Publication of UA99594C2 publication Critical patent/UA99594C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Изобретение относится к способу получения нетрансгенного растения, стойкого к гербициду фосфонометилглициновой группы, например, глифосата, путем введения в растительные клетки рекомбинагенной олигонуклеоосновы с направленной мутацией а гене EPSPS для получения растительных клеток с мутегенным геном EPSPS, который экспрессирует белок EPSPS, который является мутантным в аминокислотных позициях Thri Proв белке EPSPS Arabidopsis (AF360224), или в аналогичном аминокислотном остатке в паралоге EPSPS, где Thrзаменен на Ile, и Proзаменен на Thr и Ala. Изобретение также относится к нетрансгенным растениям, стойким к гербициду фосфонометилглициновой группы, полученным указанным способом.
UAA200810303A 2006-01-12 2007-01-10 Epsps mutants UA99594C2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US75843906P 2006-01-12 2006-01-12

Publications (1)

Publication Number Publication Date
UA99594C2 true UA99594C2 (en) 2012-09-10

Family

ID=38288103

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA200810303A UA99594C2 (en) 2006-01-12 2007-01-10 Epsps mutants

Country Status (14)

Country Link
US (3) US8268622B2 (ru)
EP (4) EP2923563B1 (ru)
JP (1) JP2009523418A (ru)
AU (1) AU2007207813B2 (ru)
CA (3) CA2923605C (ru)
DK (2) DK2465340T3 (ru)
ES (2) ES2531882T3 (ru)
NZ (4) NZ613267A (ru)
PL (2) PL2465341T3 (ru)
PT (2) PT2465341E (ru)
RU (1) RU2441366C2 (ru)
SI (2) SI2465340T1 (ru)
UA (1) UA99594C2 (ru)
WO (1) WO2007084294A2 (ru)

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DK2235187T3 (en) 2007-12-21 2014-03-03 Keygene Nv Enhanced mutagenesis using mutagenic introduction of polyethylenglycolmedieret nucleobases in plant protoplasts
PL2342331T3 (pl) 2008-09-26 2016-05-31 Basf Agrochemical Products Bv Mutanty ahas odporne na herbicydy i sposoby ich zastosowania

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EP1978799A4 (en) 2009-11-11
US20090307802A1 (en) 2009-12-10
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PT2465341E (pt) 2015-03-09
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