UA75321C2 - Химерный белок растворимого рецептора интерлейкина-6/интерлейкина-6 (sil-6r/il-6), применение данного слитого белка для получения лекарственного средства для лечения рака, заболеваний печени, неврологических заболеваний, для стимуляции кровообразования, для улучшения приживляемости клеток костного мозга при его трансплантации и фармацевтическая композиция, которая его содержит - Google Patents
Химерный белок растворимого рецептора интерлейкина-6/интерлейкина-6 (sil-6r/il-6), применение данного слитого белка для получения лекарственного средства для лечения рака, заболеваний печени, неврологических заболеваний, для стимуляции кровообразования, для улучшения приживляемости клеток костного мозга при его трансплантации и фармацевтическая композиция, которая его содержит Download PDFInfo
- Publication number
- UA75321C2 UA75321C2 UA2000020719A UA2000020719A UA75321C2 UA 75321 C2 UA75321 C2 UA 75321C2 UA 2000020719 A UA2000020719 A UA 2000020719A UA 2000020719 A UA2000020719 A UA 2000020719A UA 75321 C2 UA75321 C2 UA 75321C2
- Authority
- UA
- Ukraine
- Prior art keywords
- chimeric protein
- cells
- protein
- specified
- 6cli
- Prior art date
Links
- 102000037865 fusion proteins Human genes 0.000 title claims abstract description 165
- 108020001507 fusion proteins Proteins 0.000 title claims abstract description 165
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 55
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 54
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 19
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 18
- 201000011510 cancer Diseases 0.000 title claims abstract description 17
- 208000012902 Nervous system disease Diseases 0.000 title claims abstract description 12
- 208000019423 liver disease Diseases 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 238000002054 transplantation Methods 0.000 title claims abstract description 9
- 238000011282 treatment Methods 0.000 title claims description 27
- 210000002798 bone marrow cell Anatomy 0.000 title claims description 12
- 230000000638 stimulation Effects 0.000 title claims description 4
- 239000003814 drug Substances 0.000 title abstract description 3
- 238000000034 method Methods 0.000 claims abstract description 38
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 28
- 210000001185 bone marrow Anatomy 0.000 claims abstract description 17
- 210000004027 cell Anatomy 0.000 claims description 106
- 241000282414 Homo sapiens Species 0.000 claims description 49
- 150000001413 amino acids Chemical class 0.000 claims description 49
- 239000013598 vector Substances 0.000 claims description 43
- 108020004414 DNA Proteins 0.000 claims description 36
- 210000005260 human cell Anatomy 0.000 claims description 24
- 210000004962 mammalian cell Anatomy 0.000 claims description 23
- 230000012010 growth Effects 0.000 claims description 19
- 201000001441 melanoma Diseases 0.000 claims description 17
- 239000013612 plasmid Substances 0.000 claims description 17
- 238000010322 bone marrow transplantation Methods 0.000 claims description 16
- 125000000539 amino acid group Chemical group 0.000 claims description 15
- 239000001963 growth medium Substances 0.000 claims description 14
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 13
- 230000004083 survival effect Effects 0.000 claims description 12
- 229940126601 medicinal product Drugs 0.000 claims description 11
- 241000701022 Cytomegalovirus Species 0.000 claims description 10
- 208000025966 Neurological disease Diseases 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 210000004081 cilia Anatomy 0.000 claims description 8
- 230000011132 hemopoiesis Effects 0.000 claims description 8
- 102000003886 Glycoproteins Human genes 0.000 claims description 7
- 108090000288 Glycoproteins Proteins 0.000 claims description 7
- 210000004899 c-terminal region Anatomy 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 7
- 239000004480 active ingredient Substances 0.000 claims description 6
- 230000002163 immunogen Effects 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 230000002401 inhibitory effect Effects 0.000 claims description 5
- 241000699802 Cricetulus griseus Species 0.000 claims description 4
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 4
- 208000035250 cutaneous malignant susceptibility to 1 melanoma Diseases 0.000 claims description 4
- 239000000945 filler Substances 0.000 claims description 4
- 210000004185 liver Anatomy 0.000 claims description 4
- 210000001672 ovary Anatomy 0.000 claims description 4
- 230000004936 stimulating effect Effects 0.000 claims description 4
- 238000004587 chromatography analysis Methods 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 claims description 3
- 239000003937 drug carrier Substances 0.000 claims description 3
- 230000003211 malignant effect Effects 0.000 claims description 3
- 230000028327 secretion Effects 0.000 claims description 3
- 231100000334 hepatotoxic Toxicity 0.000 claims description 2
- 230000003082 hepatotoxic effect Effects 0.000 claims description 2
- 210000003734 kidney Anatomy 0.000 claims description 2
- 108090001005 Interleukin-6 Proteins 0.000 abstract description 3
- 230000021615 conjugation Effects 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 description 48
- 235000018102 proteins Nutrition 0.000 description 39
- 230000000694 effects Effects 0.000 description 32
- 241000699670 Mus sp. Species 0.000 description 27
- 230000004071 biological effect Effects 0.000 description 17
- 239000000203 mixture Substances 0.000 description 17
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 15
- 150000003839 salts Chemical class 0.000 description 15
- 210000000130 stem cell Anatomy 0.000 description 14
- 125000003275 alpha amino acid group Chemical group 0.000 description 12
- 239000000047 product Substances 0.000 description 11
- 102000005962 receptors Human genes 0.000 description 11
- 108020003175 receptors Proteins 0.000 description 11
- 238000007792 addition Methods 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 9
- 239000003446 ligand Substances 0.000 description 9
- 102000004127 Cytokines Human genes 0.000 description 8
- 108090000695 Cytokines Proteins 0.000 description 8
- 230000004927 fusion Effects 0.000 description 8
- 230000013595 glycosylation Effects 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- 238000002347 injection Methods 0.000 description 8
- 108020004707 nucleic acids Proteins 0.000 description 8
- 102000039446 nucleic acids Human genes 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 238000006467 substitution reaction Methods 0.000 description 8
- 238000010276 construction Methods 0.000 description 7
- 238000006206 glycosylation reaction Methods 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 102000004196 processed proteins & peptides Human genes 0.000 description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 102000003675 cytokine receptors Human genes 0.000 description 6
- 108010057085 cytokine receptors Proteins 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000001962 electrophoresis Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 230000035755 proliferation Effects 0.000 description 6
- 108020004705 Codon Proteins 0.000 description 5
- 108010076504 Protein Sorting Signals Proteins 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 206010028851 Necrosis Diseases 0.000 description 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 125000003178 carboxy group Chemical class [H]OC(*)=O 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 4
- 208000035475 disorder Diseases 0.000 description 4
- 210000000720 eyelash Anatomy 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 230000017074 necrotic cell death Effects 0.000 description 4
- 229920001184 polypeptide Polymers 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000004114 suspension culture Methods 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 3
- 206010027480 Metastatic malignant melanoma Diseases 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 210000000988 bone and bone Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000034994 death Effects 0.000 description 3
- 239000010432 diamond Substances 0.000 description 3
- 239000002552 dosage form Substances 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 208000021039 metastatic melanoma Diseases 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 239000002751 oligonucleotide probe Substances 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 102000004058 Leukemia inhibitory factor Human genes 0.000 description 2
- 108090000581 Leukemia inhibitory factor Proteins 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- NQRYJNQNLNOLGT-UHFFFAOYSA-N Piperidine Chemical compound C1CCNCC1 NQRYJNQNLNOLGT-UHFFFAOYSA-N 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 235000011054 acetic acid Nutrition 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 208000027697 autoimmune lymphoproliferative syndrome due to CTLA4 haploinsuffiency Diseases 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 230000005907 cancer growth Effects 0.000 description 2
- 229950005499 carbon tetrachloride Drugs 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000001332 colony forming effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 210000004700 fetal blood Anatomy 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 238000001415 gene therapy Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 102000035122 glycosylated proteins Human genes 0.000 description 2
- 108091005608 glycosylated proteins Proteins 0.000 description 2
- 230000009422 growth inhibiting effect Effects 0.000 description 2
- 231100000304 hepatotoxicity Toxicity 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000001990 intravenous administration Methods 0.000 description 2
- 210000003292 kidney cell Anatomy 0.000 description 2
- 210000005228 liver tissue Anatomy 0.000 description 2
- 206010061289 metastatic neoplasm Diseases 0.000 description 2
- JLESVLCTIOAHPT-UHFFFAOYSA-N mmai Chemical compound C1=C(C)C(OC)=CC2=C1CC(N)C2 JLESVLCTIOAHPT-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004660 morphological change Effects 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 229960005489 paracetamol Drugs 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 230000004481 post-translational protein modification Effects 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000002629 repopulating effect Effects 0.000 description 2
- 230000001177 retroviral effect Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- AYNDKKQQUZPETC-NXVVXOECSA-N (z)-2,3-bis(2,4,5-trimethylthiophen-3-yl)but-2-enedinitrile Chemical compound CC1=C(C)SC(C)=C1\C(C#N)=C(\C#N)C1=C(C)SC(C)=C1C AYNDKKQQUZPETC-NXVVXOECSA-N 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 102100034612 Annexin A4 Human genes 0.000 description 1
- 108090000669 Annexin A4 Proteins 0.000 description 1
- 101100262446 Arabidopsis thaliana UBA1 gene Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000283725 Bos Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 101100476202 Caenorhabditis elegans mog-2 gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 206010010099 Combined immunodeficiency Diseases 0.000 description 1
- 244000102216 Crateva tapia Species 0.000 description 1
- 235000003495 Crateva tapia Nutrition 0.000 description 1
- 244000019459 Cynara cardunculus Species 0.000 description 1
- 235000019106 Cynara scolymus Nutrition 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000003951 Erythropoietin Human genes 0.000 description 1
- 108090000394 Erythropoietin Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
- 206010019799 Hepatitis viral Diseases 0.000 description 1
- 206010019851 Hepatotoxicity Diseases 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000016844 Immunoglobulin-like domains Human genes 0.000 description 1
- 108050006430 Immunoglobulin-like domains Proteins 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- 102100032352 Leukemia inhibitory factor Human genes 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 101150033052 MAS5 gene Proteins 0.000 description 1
- 206010027458 Metastases to lung Diseases 0.000 description 1
- 101100310622 Mus musculus Soga1 gene Proteins 0.000 description 1
- OKIZCWYLBDKLSU-UHFFFAOYSA-M N,N,N-Trimethylmethanaminium chloride Chemical compound [Cl-].C[N+](C)(C)C OKIZCWYLBDKLSU-UHFFFAOYSA-M 0.000 description 1
- 230000004989 O-glycosylation Effects 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 102000004140 Oncostatin M Human genes 0.000 description 1
- 108090000630 Oncostatin M Proteins 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 102000004278 Receptor Protein-Tyrosine Kinases Human genes 0.000 description 1
- 108090000873 Receptor Protein-Tyrosine Kinases Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 101100453651 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) URA6 gene Proteins 0.000 description 1
- 101100344462 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YDJ1 gene Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000289690 Xenarthra Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 150000001243 acetic acids Chemical class 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000000556 agonist Substances 0.000 description 1
- 230000001270 agonistic effect Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000006229 amino acid addition Effects 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000002001 anti-metastasis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000016520 artichoke thistle Nutrition 0.000 description 1
- 238000000211 autoradiogram Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 201000008275 breast carcinoma Diseases 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001886 ciliary effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000005016 dendritic process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000925 erythroid effect Effects 0.000 description 1
- 229940105423 erythropoietin Drugs 0.000 description 1
- -1 etc. Chemical class 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 230000007686 hepatotoxicity Effects 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 210000004293 human mammary gland Anatomy 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 230000009851 immunogenic response Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000007056 liver toxicity Effects 0.000 description 1
- 238000011866 long-term treatment Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 201000005296 lung carcinoma Diseases 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 108020004084 membrane receptors Proteins 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 210000004498 neuroglial cell Anatomy 0.000 description 1
- 239000003076 neurotropic agent Substances 0.000 description 1
- 230000002276 neurotropic effect Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 210000004248 oligodendroglia Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 150000007530 organic bases Chemical class 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 150000002913 oxalic acids Chemical class 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 235000013550 pizza Nutrition 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 1
- MFDFERRIHVXMIY-UHFFFAOYSA-N procaine Chemical compound CCN(CC)CCOC(=O)C1=CC=C(N)C=C1 MFDFERRIHVXMIY-UHFFFAOYSA-N 0.000 description 1
- 229960004919 procaine Drugs 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 208000002491 severe combined immunodeficiency Diseases 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid group Chemical class S(O)(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000011477 surgical intervention Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000003954 umbilical cord Anatomy 0.000 description 1
- 125000002987 valine group Chemical class [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 201000001862 viral hepatitis Diseases 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 150000003751 zinc Chemical class 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Изобретение относится к химерным белкам, которые получают путем слияния природной формы растворимого рецептора IL-6 и IL-6, где обе формы гликозилированы таким способом, как и природные формы данных белков. Отмеченные химерные белки могут быть также слиты через молекулу пептидного линкера. Изобретение также относится к применению данных белков для получения лекарственного средства для лечения различных видов рака, болезней печени, неврологических нарушений, улучшения приживляемости клеток при трансплантации костного мозга и фармацевтической композиции, которые их содержат.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL12128497A IL121284A0 (en) | 1997-07-10 | 1997-07-10 | Chimeric interleukin-6 soluble receptor/ligand protein analogs thereof and uses thereof |
PCT/IL1998/000321 WO1999002552A2 (en) | 1997-07-10 | 1998-07-09 | Chimeric interleukin-6 soluble receptor/ligand protein, analogs thereof and uses thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
UA75321C2 true UA75321C2 (ru) | 2006-04-17 |
Family
ID=11070372
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UA2000020719A UA75321C2 (ru) | 1997-07-10 | 1998-09-07 | Химерный белок растворимого рецептора интерлейкина-6/интерлейкина-6 (sil-6r/il-6), применение данного слитого белка для получения лекарственного средства для лечения рака, заболеваний печени, неврологических заболеваний, для стимуляции кровообразования, для улучшения приживляемости клеток костного мозга при его трансплантации и фармацевтическая композиция, которая его содержит |
Country Status (3)
Country | Link |
---|---|
IL (1) | IL121284A0 (ru) |
UA (1) | UA75321C2 (ru) |
ZA (1) | ZA986145B (ru) |
-
1997
- 1997-07-10 IL IL12128497A patent/IL121284A0/xx unknown
-
1998
- 1998-07-10 ZA ZA986145A patent/ZA986145B/xx unknown
- 1998-09-07 UA UA2000020719A patent/UA75321C2/ru unknown
Also Published As
Publication number | Publication date |
---|---|
ZA986145B (en) | 1999-04-07 |
IL121284A0 (en) | 1998-01-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP7087047B2 (ja) | 改変されたインターロイキン-7タンパク質およびその使用 | |
EA004793B1 (ru) | Химерный белок растворимого рецептора интерлейкина-6/лиганда, его аналоги и способы применения | |
RU2179034C2 (ru) | ЛЕЧЕНИЕ В СЛУЧАЕ ДЕФИЦИТА α-ГАЛАКТОЗИДАЗЫ А | |
TWI454482B (zh) | 在活體中具有延長之半生期以及提高之紅血球生成活性的重組人類紅血球生成素(epo)-fc融合蛋白 | |
JP5558213B2 (ja) | 貧血の予防および治療用の方法および組成物 | |
ES2281704T3 (es) | Procedimientos y compuestos para inhibir el crecimiento de celulas neoplasicas. | |
US8163520B2 (en) | Nucleic acids encoding a chimeric polypeptide comprising IL-7 and HGF-beta chain and methods of use | |
US7304150B1 (en) | Methods and compositions for the prevention and treatment of anemia | |
PT95524B (pt) | Novos factores poliptidicos com actividade estimuladora de celulas indiferenciadas, processo para a sua preparacao e para a preparacao de composicoes farmaceuticas que os contem | |
BRPI0814465B1 (pt) | Proteína de fusão, dímero, método para produzir uma proteína de fusão, linhagem de célula, usos de uma proteína de fusão e de uma composição farmacêutica e composição farmacêutica | |
CA2311681A1 (en) | Human interferon-epsilon: a type i interferon | |
JP2002514887A (ja) | トロンボポイエチンポリペプチドの分泌方法 | |
JPH04501421A (ja) | 血小板産生刺激薬剤のためのil―7の使用 | |
CA2149763A1 (en) | Progenitor b cell stimulating factor | |
CN106397569B (zh) | 一种治疗代谢疾病的突变细胞因子融合蛋白 | |
DE69524093T2 (de) | Kombination von prokoagulans und cytokin oder induktor seiner produktion für die behandlung von tumoren | |
JP4280444B2 (ja) | 腫瘍性細胞成長阻害のための組成物及び方法 | |
UA75321C2 (ru) | Химерный белок растворимого рецептора интерлейкина-6/интерлейкина-6 (sil-6r/il-6), применение данного слитого белка для получения лекарственного средства для лечения рака, заболеваний печени, неврологических заболеваний, для стимуляции кровообразования, для улучшения приживляемости клеток костного мозга при его трансплантации и фармацевтическая композиция, которая его содержит | |
RU2186783C2 (ru) | Кроличья антисыворотка, ингибирующая транспорт катионных аминокислот, и содержащая ее фармацевтическая композиция | |
CN112724263B (zh) | 改造抗cd20单克隆抗体以提高其药物疗效的方法及其应用 | |
JP3646927B2 (ja) | 生体内血小板増殖効能が向上した新規なヒトトロンボポイエチン誘導体 | |
CN100460015C (zh) | 免疫调节剂及其应用 | |
JP2002529516A (ja) | CD66aを用いて新脈管形成に影響を及ぼす方法 | |
RO118299B1 (ro) | Polipeptida trombopoietina, secventa adn care o codifica, procedeu pentru prepararea polipeptidei trombopoietine si compozitie farmaceutica cu aceasta | |
CN115590976A (zh) | 治疗和/或预防hiv相关疾病的apc偶联物及其制法和应用 |