UA105459C2 - Композиция и способ получения энтерокиназы в дрожжах - Google Patents
Композиция и способ получения энтерокиназы в дрожжах Download PDFInfo
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- UA105459C2 UA105459C2 UAA201307761A UAA201307761A UA105459C2 UA 105459 C2 UA105459 C2 UA 105459C2 UA A201307761 A UAA201307761 A UA A201307761A UA A201307761 A UAA201307761 A UA A201307761A UA 105459 C2 UA105459 C2 UA 105459C2
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- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
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- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
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- C12N1/14—Fungi; Culture media therefor
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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Abstract
Изобретение относится к изолированной полинуклеотидной молекулы, которая кодирует энтерокиназу, дрожжевому экспрессионному конструкту, который включает дрожжевой вектор экспрессии и полинуклеотидные молекулы, кодирующие энтерокиназу, к дрожжевой клетке, содержащей такой экспрессионный конструкт, способу продуцирования энтерокиназы с использованием таких дрожжевых клеток, и способу расщепления или получения рекомбинантного полипептида с использованием энтерокиназы, которая продуцируется с помощью таких способов.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41662210P | 2010-11-23 | 2010-11-23 | |
| PCT/US2011/061334 WO2012071257A1 (en) | 2010-11-23 | 2011-11-18 | Compositions and methods of producing enterokinase in yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA105459C2 true UA105459C2 (ru) | 2014-05-12 |
Family
ID=45034208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAA201307761A UA105459C2 (ru) | 2010-11-23 | 2011-11-18 | Композиция и способ получения энтерокиназы в дрожжах |
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| EP (1) | EP2643457B1 (ru) |
| JP (1) | JP5425350B2 (ru) |
| KR (1) | KR101920094B1 (ru) |
| CN (1) | CN103282490B (ru) |
| AU (1) | AU2011332131B2 (ru) |
| BR (1) | BR112013012565A2 (ru) |
| CA (1) | CA2816217C (ru) |
| CL (1) | CL2013001476A1 (ru) |
| DK (1) | DK2643457T3 (ru) |
| ES (1) | ES2553149T3 (ru) |
| HK (1) | HK1189028A1 (ru) |
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| RU (1) | RU2560577C2 (ru) |
| SG (1) | SG190316A1 (ru) |
| UA (1) | UA105459C2 (ru) |
| WO (1) | WO2012071257A1 (ru) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SK500482016A3 (sk) | 2016-07-29 | 2018-02-05 | Univerzita Komenského v Bratislave | Expresná DNA kazeta nesúca gén kódujúci ľahký reťazec ľudskej enterokinázy, kmeň Pichia pastoris nesúci uvedenú expresnú DNA kazetu a spôsob jeho kultivácie |
| RU2716975C1 (ru) * | 2019-01-09 | 2020-03-17 | Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | Рекомбинантная плазмидная днк, кодирующая гибридный белок l-hep, штамм escherichia coli продуцент указанного белка и способ получения рекомбинантного белка |
Family Cites Families (8)
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| US6746859B1 (en) | 1993-01-15 | 2004-06-08 | Genetics Institute, Llc | Cloning of enterokinase and method of use |
| US7825233B2 (en) | 2004-06-30 | 2010-11-02 | Allergan, Inc. | Optimizing expression of active Botulinum Toxin type E |
| US20110111483A1 (en) * | 2004-06-30 | 2011-05-12 | Allergan, Inc. | Optimizing Expression of Active Botulinum Toxin Type E |
| JP2008508886A (ja) | 2004-08-04 | 2008-03-27 | アラーガン、インコーポレイテッド | 活性ボツリヌス毒素ａ型の発現の最適化 |
| US20070281198A1 (en) | 2006-06-01 | 2007-12-06 | Lousenberg Robert D | Membranes electrode assemblies prepared from fluoropolymer dispersions |
| US8013137B2 (en) | 2006-10-17 | 2011-09-06 | National University Corporation Hokkaido University | Modified enteropeptidase protein |
| WO2008136014A1 (en) * | 2007-05-07 | 2008-11-13 | Usv Limited | Cloning and expression of enterokinase and its process for production |
| CN102061302B (zh) * | 2010-11-26 | 2012-07-04 | 扬子江药业集团北京海燕药业有限公司 | 肠激酶轻链基因的合成及其表达产物的制备方法 |
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Also Published As
| Publication number | Publication date |
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| CA2816217C (en) | 2014-07-29 |
| DK2643457T3 (en) | 2015-11-30 |
| CA2816217A1 (en) | 2012-05-31 |
| ES2553149T3 (es) | 2015-12-04 |
| US20130302853A1 (en) | 2013-11-14 |
| EP2643457A1 (en) | 2013-10-02 |
| KR20140018850A (ko) | 2014-02-13 |
| CN103282490A (zh) | 2013-09-04 |
| IL226165A0 (en) | 2013-06-27 |
| JP5425350B2 (ja) | 2014-02-26 |
| JP2014501511A (ja) | 2014-01-23 |
| MY170261A (en) | 2019-07-13 |
| AU2011332131A1 (en) | 2013-07-04 |
| RU2560577C2 (ru) | 2015-08-20 |
| HK1189028A1 (en) | 2014-05-23 |
| WO2012071257A1 (en) | 2012-05-31 |
| BR112013012565A2 (pt) | 2016-10-25 |
| NZ611164A (en) | 2015-02-27 |
| SG190316A1 (en) | 2013-06-28 |
| CN103282490B (zh) | 2016-09-21 |
| MX2013005749A (es) | 2013-08-01 |
| US8557558B2 (en) | 2013-10-15 |
| IL226165A (en) | 2017-02-28 |
| RU2013128339A (ru) | 2014-12-27 |
| AU2011332131B2 (en) | 2015-08-13 |
| CL2013001476A1 (es) | 2014-01-31 |
| KR101920094B1 (ko) | 2018-11-19 |
| US20120156720A1 (en) | 2012-06-21 |
| EP2643457B1 (en) | 2015-08-26 |
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