TWI881970B - 含有新穎選擇標記的細胞株及其用於蛋白製造的用途 - Google Patents

含有新穎選擇標記的細胞株及其用於蛋白製造的用途 Download PDF

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TWI881970B
TWI881970B TW109109257A TW109109257A TWI881970B TW I881970 B TWI881970 B TW I881970B TW 109109257 A TW109109257 A TW 109109257A TW 109109257 A TW109109257 A TW 109109257A TW I881970 B TWI881970 B TW I881970B
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cell line
dhodh
sequence
seq
gene
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TW109109257A
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Chinese (zh)
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TW202102669A (zh
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布魯諾 當馬斯
穆罕默德 路易士
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法商賽諾菲公司
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
    • C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
TW109109257A 2019-03-19 2020-03-19 含有新穎選擇標記的細胞株及其用於蛋白製造的用途 TWI881970B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19305331.1 2019-03-19
EP19305331 2019-03-19

Publications (2)

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TW202102669A TW202102669A (zh) 2021-01-16
TWI881970B true TWI881970B (zh) 2025-05-01

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TW109109257A TWI881970B (zh) 2019-03-19 2020-03-19 含有新穎選擇標記的細胞株及其用於蛋白製造的用途

Country Status (14)

Country Link
US (1) US12529071B2 (https=)
EP (1) EP3942054A1 (https=)
JP (1) JP7609795B2 (https=)
KR (1) KR20210141511A (https=)
CN (1) CN113811615B (https=)
AU (1) AU2020242966B2 (https=)
BR (1) BR112021018124A2 (https=)
CA (1) CA3134116A1 (https=)
IL (1) IL286419A (https=)
MX (1) MX2021011303A (https=)
SG (1) SG11202110105UA (https=)
TW (1) TWI881970B (https=)
WO (1) WO2020188021A1 (https=)
ZA (1) ZA202106718B (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI912369B (zh) * 2020-09-21 2026-01-21 法商賽諾菲公司 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016062837A1 (en) * 2014-10-23 2016-04-28 Sanofi Novel selection marker for cell transfection and protein production

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GB8717430D0 (en) 1987-07-23 1987-08-26 Celltech Ltd Recombinant dna product
KR100267720B1 (ko) 1997-12-29 2001-03-02 박호군 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법
US20030166201A1 (en) * 2001-04-30 2003-09-04 Jensen Michael C. Selection systems for genetically modified cells
AU2007250735A1 (en) * 2006-05-16 2007-11-22 Daiichi Sankyo Company, Limited Method for high-level secretory production of protein
US9234210B2 (en) 2010-08-25 2016-01-12 Intrexon Ceu, Inc. Selectable markers and related methods
RU2556816C1 (ru) 2014-03-12 2015-07-20 Открытое акционерное общество "Фармацевтический импорт, экспорт" (ОАО "Фармимэкс") Штамм клеток яичников китайского хомячка - продуцент рекомбинантного антитела против фактора некроза опухоли альфа человека
US11096934B2 (en) 2015-09-01 2021-08-24 The Broad Institute, Inc. Compounds and methods useful for treating or preventing hematological cancers
US10736911B2 (en) * 2015-12-29 2020-08-11 The Board Of Trustees Of The Leland Stanford Junior University Use of a DHODH inhibitor in combination with an inhibitor of pyrimidine salvage
CN109777837A (zh) 2018-12-26 2019-05-21 首都医科大学 一种利用CRISPR/Cas9系统构建致死基因全身性敲除小鼠模型的方法
TWI912369B (zh) 2020-09-21 2026-01-21 法商賽諾菲公司 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016062837A1 (en) * 2014-10-23 2016-04-28 Sanofi Novel selection marker for cell transfection and protein production

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
期刊 Bajzikova et al. Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells Cell Metab 29(2) 2019 Feb 5 1-50 *

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Publication number Publication date
AU2020242966A1 (en) 2021-10-21
CN113811615B (zh) 2025-11-11
TW202102669A (zh) 2021-01-16
JP7609795B2 (ja) 2025-01-07
CA3134116A1 (en) 2020-09-24
ZA202106718B (en) 2023-04-26
EP3942054A1 (en) 2022-01-26
MX2021011303A (es) 2021-10-22
US12529071B2 (en) 2026-01-20
AU2020242966B2 (en) 2025-09-25
BR112021018124A2 (pt) 2021-11-16
CN113811615A (zh) 2021-12-17
KR20210141511A (ko) 2021-11-23
IL286419A (en) 2021-10-31
WO2020188021A1 (en) 2020-09-24
US20220177909A1 (en) 2022-06-09
SG11202110105UA (en) 2021-10-28
JP2022526497A (ja) 2022-05-25

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