CA3134116A1 - Cell lines and selection markers for protein production - Google Patents
Cell lines and selection markers for protein production Download PDFInfo
- Publication number
- CA3134116A1 CA3134116A1 CA3134116A CA3134116A CA3134116A1 CA 3134116 A1 CA3134116 A1 CA 3134116A1 CA 3134116 A CA3134116 A CA 3134116A CA 3134116 A CA3134116 A CA 3134116A CA 3134116 A1 CA3134116 A1 CA 3134116A1
- Authority
- CA
- Canada
- Prior art keywords
- dhodh
- cell line
- gene
- sequence
- cho
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
- C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19305331.1 | 2019-03-19 | ||
| EP19305331 | 2019-03-19 | ||
| PCT/EP2020/057583 WO2020188021A1 (en) | 2019-03-19 | 2020-03-19 | Novel selection marker-comprising cell line and uses thereof for protein production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3134116A1 true CA3134116A1 (en) | 2020-09-24 |
Family
ID=66001151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3134116A Pending CA3134116A1 (en) | 2019-03-19 | 2020-03-19 | Cell lines and selection markers for protein production |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US12529071B2 (https=) |
| EP (1) | EP3942054A1 (https=) |
| JP (1) | JP7609795B2 (https=) |
| KR (1) | KR20210141511A (https=) |
| CN (1) | CN113811615B (https=) |
| AU (1) | AU2020242966B2 (https=) |
| BR (1) | BR112021018124A2 (https=) |
| CA (1) | CA3134116A1 (https=) |
| IL (1) | IL286419A (https=) |
| MX (1) | MX2021011303A (https=) |
| SG (1) | SG11202110105UA (https=) |
| TW (1) | TWI881970B (https=) |
| WO (1) | WO2020188021A1 (https=) |
| ZA (1) | ZA202106718B (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI912369B (zh) * | 2020-09-21 | 2026-01-21 | 法商賽諾菲公司 | 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
| KR100267720B1 (ko) | 1997-12-29 | 2001-03-02 | 박호군 | 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법 |
| US20030166201A1 (en) * | 2001-04-30 | 2003-09-04 | Jensen Michael C. | Selection systems for genetically modified cells |
| AU2007250735A1 (en) * | 2006-05-16 | 2007-11-22 | Daiichi Sankyo Company, Limited | Method for high-level secretory production of protein |
| US9234210B2 (en) | 2010-08-25 | 2016-01-12 | Intrexon Ceu, Inc. | Selectable markers and related methods |
| RU2556816C1 (ru) | 2014-03-12 | 2015-07-20 | Открытое акционерное общество "Фармацевтический импорт, экспорт" (ОАО "Фармимэкс") | Штамм клеток яичников китайского хомячка - продуцент рекомбинантного антитела против фактора некроза опухоли альфа человека |
| AU2015334872B2 (en) | 2014-10-23 | 2021-12-16 | Sanofi | Novel selection marker for cell transfection and protein production |
| US11096934B2 (en) | 2015-09-01 | 2021-08-24 | The Broad Institute, Inc. | Compounds and methods useful for treating or preventing hematological cancers |
| US10736911B2 (en) * | 2015-12-29 | 2020-08-11 | The Board Of Trustees Of The Leland Stanford Junior University | Use of a DHODH inhibitor in combination with an inhibitor of pyrimidine salvage |
| CN109777837A (zh) | 2018-12-26 | 2019-05-21 | 首都医科大学 | 一种利用CRISPR/Cas9系统构建致死基因全身性敲除小鼠模型的方法 |
| TWI912369B (zh) | 2020-09-21 | 2026-01-21 | 法商賽諾菲公司 | 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代 |
-
2020
- 2020-03-19 US US17/440,728 patent/US12529071B2/en active Active
- 2020-03-19 SG SG11202110105UA patent/SG11202110105UA/en unknown
- 2020-03-19 WO PCT/EP2020/057583 patent/WO2020188021A1/en not_active Ceased
- 2020-03-19 MX MX2021011303A patent/MX2021011303A/es unknown
- 2020-03-19 KR KR1020217029997A patent/KR20210141511A/ko active Pending
- 2020-03-19 CA CA3134116A patent/CA3134116A1/en active Pending
- 2020-03-19 AU AU2020242966A patent/AU2020242966B2/en active Active
- 2020-03-19 TW TW109109257A patent/TWI881970B/zh active
- 2020-03-19 EP EP20710976.0A patent/EP3942054A1/en active Pending
- 2020-03-19 JP JP2021556438A patent/JP7609795B2/ja active Active
- 2020-03-19 CN CN202080035132.1A patent/CN113811615B/zh active Active
- 2020-03-19 BR BR112021018124A patent/BR112021018124A2/pt unknown
-
2021
- 2021-09-10 ZA ZA2021/06718A patent/ZA202106718B/en unknown
- 2021-09-14 IL IL286419A patent/IL286419A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020242966A1 (en) | 2021-10-21 |
| CN113811615B (zh) | 2025-11-11 |
| TW202102669A (zh) | 2021-01-16 |
| JP7609795B2 (ja) | 2025-01-07 |
| TWI881970B (zh) | 2025-05-01 |
| ZA202106718B (en) | 2023-04-26 |
| EP3942054A1 (en) | 2022-01-26 |
| MX2021011303A (es) | 2021-10-22 |
| US12529071B2 (en) | 2026-01-20 |
| AU2020242966B2 (en) | 2025-09-25 |
| BR112021018124A2 (pt) | 2021-11-16 |
| CN113811615A (zh) | 2021-12-17 |
| KR20210141511A (ko) | 2021-11-23 |
| IL286419A (en) | 2021-10-31 |
| WO2020188021A1 (en) | 2020-09-24 |
| US20220177909A1 (en) | 2022-06-09 |
| SG11202110105UA (en) | 2021-10-28 |
| JP2022526497A (ja) | 2022-05-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12203103B2 (en) | Selection marker for cell transfection and protein production | |
| US12529071B2 (en) | Selection marker-comprising cell line and uses thereof for protein production | |
| EP4214308B1 (en) | Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production | |
| RU2820830C2 (ru) | Линия клеток, содержащая новый маркер отбора, и ее применение для продуцирования белков | |
| RS61371B1 (sr) | Efikasna selektivnost rekombinantnih proteina | |
| RU2849601C1 (ru) | Получение рекомбинантной линии клеток китайского хомячка, характеризующейся высоким продуцированием, для продуцирования терапевтических белков | |
| HK40096091A (en) | Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production | |
| HK40096091B (en) | Generation of a high producing recombinant chinese hamster ovary cell line for therapeutic protein production |
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