MX2021011303A - Nueva linea celular que comprende marcador de seleccion y usos de la misma para la produccion de proteinas. - Google Patents

Nueva linea celular que comprende marcador de seleccion y usos de la misma para la produccion de proteinas.

Info

Publication number
MX2021011303A
MX2021011303A MX2021011303A MX2021011303A MX2021011303A MX 2021011303 A MX2021011303 A MX 2021011303A MX 2021011303 A MX2021011303 A MX 2021011303A MX 2021011303 A MX2021011303 A MX 2021011303A MX 2021011303 A MX2021011303 A MX 2021011303A
Authority
MX
Mexico
Prior art keywords
cell line
selection marker
protein production
novel selection
novel
Prior art date
Application number
MX2021011303A
Other languages
English (en)
Inventor
Bruno Louis Dumas
Mohammed Nabil Lounis
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of MX2021011303A publication Critical patent/MX2021011303A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
    • C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a una línea celular que comprende un gen endógeno de deshidrogenasa de dihidroorotato (DHODH, por sus siglas en inglés) que se inactiva de una forma parcial o total, y a su uso para la producción de las proteínas recombinantes.
MX2021011303A 2019-03-19 2020-03-19 Nueva linea celular que comprende marcador de seleccion y usos de la misma para la produccion de proteinas. MX2021011303A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19305331 2019-03-19
PCT/EP2020/057583 WO2020188021A1 (en) 2019-03-19 2020-03-19 Novel selection marker-comprising cell line and uses thereof for protein production

Publications (1)

Publication Number Publication Date
MX2021011303A true MX2021011303A (es) 2021-10-22

Family

ID=66001151

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021011303A MX2021011303A (es) 2019-03-19 2020-03-19 Nueva linea celular que comprende marcador de seleccion y usos de la misma para la produccion de proteinas.

Country Status (14)

Country Link
US (1) US20220177909A1 (es)
EP (1) EP3942054A1 (es)
JP (1) JP2022526497A (es)
KR (1) KR20210141511A (es)
CN (1) CN113811615A (es)
AU (1) AU2020242966A1 (es)
BR (1) BR112021018124A2 (es)
CA (1) CA3134116A1 (es)
IL (1) IL286419A (es)
MX (1) MX2021011303A (es)
SG (1) SG11202110105UA (es)
TW (1) TW202102669A (es)
WO (1) WO2020188021A1 (es)
ZA (1) ZA202106718B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202227622A (zh) * 2020-09-21 2022-07-16 法商賽諾菲公司 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8717430D0 (en) 1987-07-23 1987-08-26 Celltech Ltd Recombinant dna product
KR100267720B1 (ko) 1997-12-29 2001-03-02 박호군 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법
MX2017005295A (es) 2014-10-23 2017-07-28 Sanofi Sa Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas.
WO2017037022A1 (en) * 2015-09-01 2017-03-09 Bayer Pharma Aktiengesellschaft Compounds and methods useful for treating or preventing hematological cancers
CN109777837A (zh) * 2018-12-26 2019-05-21 首都医科大学 一种利用CRISPR/Cas9系统构建致死基因全身性敲除小鼠模型的方法
TW202227622A (zh) * 2020-09-21 2022-07-16 法商賽諾菲公司 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代

Also Published As

Publication number Publication date
WO2020188021A1 (en) 2020-09-24
ZA202106718B (en) 2023-04-26
US20220177909A1 (en) 2022-06-09
SG11202110105UA (en) 2021-10-28
EP3942054A1 (en) 2022-01-26
CA3134116A1 (en) 2020-09-24
JP2022526497A (ja) 2022-05-25
KR20210141511A (ko) 2021-11-23
IL286419A (en) 2021-10-31
TW202102669A (zh) 2021-01-16
BR112021018124A2 (pt) 2021-11-16
CN113811615A (zh) 2021-12-17
AU2020242966A1 (en) 2021-10-21

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