AU2020242966B2 - Novel selection marker-comprising cell line and uses thereof for protein production - Google Patents

Novel selection marker-comprising cell line and uses thereof for protein production

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Publication number
AU2020242966B2
AU2020242966B2 AU2020242966A AU2020242966A AU2020242966B2 AU 2020242966 B2 AU2020242966 B2 AU 2020242966B2 AU 2020242966 A AU2020242966 A AU 2020242966A AU 2020242966 A AU2020242966 A AU 2020242966A AU 2020242966 B2 AU2020242966 B2 AU 2020242966B2
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AU
Australia
Prior art keywords
dhodh
cell line
expression
gene
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2020242966A
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English (en)
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AU2020242966A1 (en
Inventor
Bruno Louis DUMAS
Mohammed Nabil LOUNIS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi SA
Original Assignee
Sanofi SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Sanofi SA filed Critical Sanofi SA
Publication of AU2020242966A1 publication Critical patent/AU2020242966A1/en
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Publication of AU2020242966B2 publication Critical patent/AU2020242966B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
    • C12Y103/05002—Dihydroorotate dehydrogenase (1.3.5.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2020242966A 2019-03-19 2020-03-19 Novel selection marker-comprising cell line and uses thereof for protein production Active AU2020242966B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19305331.1 2019-03-19
EP19305331 2019-03-19
PCT/EP2020/057583 WO2020188021A1 (en) 2019-03-19 2020-03-19 Novel selection marker-comprising cell line and uses thereof for protein production

Publications (2)

Publication Number Publication Date
AU2020242966A1 AU2020242966A1 (en) 2021-10-21
AU2020242966B2 true AU2020242966B2 (en) 2025-09-25

Family

ID=66001151

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020242966A Active AU2020242966B2 (en) 2019-03-19 2020-03-19 Novel selection marker-comprising cell line and uses thereof for protein production

Country Status (14)

Country Link
US (1) US12529071B2 (https=)
EP (1) EP3942054A1 (https=)
JP (1) JP7609795B2 (https=)
KR (1) KR20210141511A (https=)
CN (1) CN113811615B (https=)
AU (1) AU2020242966B2 (https=)
BR (1) BR112021018124A2 (https=)
CA (1) CA3134116A1 (https=)
IL (1) IL286419A (https=)
MX (1) MX2021011303A (https=)
SG (1) SG11202110105UA (https=)
TW (1) TWI881970B (https=)
WO (1) WO2020188021A1 (https=)
ZA (1) ZA202106718B (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI912369B (zh) * 2020-09-21 2026-01-21 法商賽諾菲公司 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007132949A1 (ja) * 2006-05-16 2007-11-22 National Institute Of Advanced Industrial Science And Technology タンパク質の高分泌生産方法
WO2012027533A1 (en) * 2010-08-25 2012-03-01 Gt Life Sciences, Inc. Selectable markers and related methods
WO2016062837A1 (en) * 2014-10-23 2016-04-28 Sanofi Novel selection marker for cell transfection and protein production

Family Cites Families (8)

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Publication number Priority date Publication date Assignee Title
GB8717430D0 (en) 1987-07-23 1987-08-26 Celltech Ltd Recombinant dna product
KR100267720B1 (ko) 1997-12-29 2001-03-02 박호군 사람 글루타민 합성효소 유전자를 포함하는 발현벡터 및 이를 이용하여 동물세포에서 에리트로포이에틴을 고수율로 생산하는 방법
US20030166201A1 (en) * 2001-04-30 2003-09-04 Jensen Michael C. Selection systems for genetically modified cells
RU2556816C1 (ru) 2014-03-12 2015-07-20 Открытое акционерное общество "Фармацевтический импорт, экспорт" (ОАО "Фармимэкс") Штамм клеток яичников китайского хомячка - продуцент рекомбинантного антитела против фактора некроза опухоли альфа человека
US11096934B2 (en) 2015-09-01 2021-08-24 The Broad Institute, Inc. Compounds and methods useful for treating or preventing hematological cancers
US10736911B2 (en) * 2015-12-29 2020-08-11 The Board Of Trustees Of The Leland Stanford Junior University Use of a DHODH inhibitor in combination with an inhibitor of pyrimidine salvage
CN109777837A (zh) 2018-12-26 2019-05-21 首都医科大学 一种利用CRISPR/Cas9系统构建致死基因全身性敲除小鼠模型的方法
TWI912369B (zh) 2020-09-21 2026-01-21 法商賽諾菲公司 用於生產治療蛋白之高生產重組中國倉鼠卵巢細胞株之世代

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007132949A1 (ja) * 2006-05-16 2007-11-22 National Institute Of Advanced Industrial Science And Technology タンパク質の高分泌生産方法
WO2012027533A1 (en) * 2010-08-25 2012-03-01 Gt Life Sciences, Inc. Selectable markers and related methods
WO2016062837A1 (en) * 2014-10-23 2016-04-28 Sanofi Novel selection marker for cell transfection and protein production

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Bajzikova, Martina et al. 'Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells.' Cell metabolism vol. 29,2 (2019): 399-416.e10. *
MOUSE GENOME INFORMATICS: "Dhodh tm1b(EUCOMM)Wtsi", 11 June 2019 (2019-06-11), XP002792217, Retrieved from the Internet [retrieved on 20190218] *
MOUSE GENOME INFORMATICS: "Dhodh tm1e(EUCOMM)Wtsi", 11 June 2019 (2019-06-11), XP002792216, Retrieved from the Internet *
VAN DER WEYDEN L ET AL: "Genome-wide in vivo screen identifies novel host regulators of metastatic colonization (includes Methods)", NATURE, vol. 541, no. 7636, 12 January 2017 (2017-01-12), NATURE PUBLISHING GROUP, pages 233 - 236+13pp *
VAN DER WEYDEN L ET AL: "Genome-wide in vivo screen identifies novel host regulators of metastatic colonization. Supplementary information", 12 January 2017 (2017-01-12), XP002792219, Retrieved from the Internet [retrieved on 20190619] *
YAMAZAKI H ET AL: "Genetic transformation of a Rhizomucor pusillus mutant defective in asparagine-linked glycosylation: Production of a milk-clotting enzyme in a less-glycosylated form", APPL MICROBIOL BIOTECHNOL, vol. 52, no. 3, Sept 1999 *

Also Published As

Publication number Publication date
AU2020242966A1 (en) 2021-10-21
CN113811615B (zh) 2025-11-11
TW202102669A (zh) 2021-01-16
JP7609795B2 (ja) 2025-01-07
CA3134116A1 (en) 2020-09-24
TWI881970B (zh) 2025-05-01
ZA202106718B (en) 2023-04-26
EP3942054A1 (en) 2022-01-26
MX2021011303A (es) 2021-10-22
US12529071B2 (en) 2026-01-20
BR112021018124A2 (pt) 2021-11-16
CN113811615A (zh) 2021-12-17
KR20210141511A (ko) 2021-11-23
IL286419A (en) 2021-10-31
WO2020188021A1 (en) 2020-09-24
US20220177909A1 (en) 2022-06-09
SG11202110105UA (en) 2021-10-28
JP2022526497A (ja) 2022-05-25

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