TWI865753B - 雙特異性抗體及其製造方法 - Google Patents

雙特異性抗體及其製造方法 Download PDF

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TWI865753B
TWI865753B TW110111315A TW110111315A TWI865753B TW I865753 B TWI865753 B TW I865753B TW 110111315 A TW110111315 A TW 110111315A TW 110111315 A TW110111315 A TW 110111315A TW I865753 B TWI865753 B TW I865753B
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Taiwan
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amino acid
acid sequence
seq
sequence represented
variable region
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TW110111315A
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Chinese (zh)
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TW202204407A (zh
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珠玖洋
赤堀泰
田中健人
谷津彩香
市川淳也
大塚敏明
上妻志保
橋本隆二
中山麻紀子
篠崎直也
中村健介
渡邊一郎
古薗信二
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國立大學法人三重大學
日商第一三共股份有限公司
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    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2833—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against MHC-molecules, e.g. HLA-molecules
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TW110111315A 2020-03-30 2021-03-29 雙特異性抗體及其製造方法 TWI865753B (zh)

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JP2020061476 2020-03-30

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TW202204407A TW202204407A (zh) 2022-02-01
TWI865753B true TWI865753B (zh) 2024-12-11

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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7674278B2 (ja) * 2019-07-03 2025-05-09 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 抗ニューヨーク食道扁平上皮がん１（ｎｙ－ｅｓｏ－１）抗原結合タンパク質およびその使用方法
US20250195645A1 (en) * 2022-03-16 2025-06-19 Daiichi Sankyo Company, Limited Combination of multispecific molecule and immune checkpoint inhibitor
EP4536839A1 (en) * 2022-06-09 2025-04-16 Amgen Inc. Methods of screening for peptide-hla class i alloreactivity
CN120077068A (zh) * 2022-08-18 2025-05-30 英美偌科有限公司 多结构域结合分子
EP4573125A1 (en) * 2022-08-18 2025-06-25 Immunocore Limited Multi-domain binding molecules

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010106431A2 (en) * 2009-03-20 2010-09-23 Ludwig Institute For Cancer Research Ltd High affinity t-cell receptor-like ny-eso-1 peptide antibodies, methods, and uses thereof
TW201713701A (zh) * 2015-06-24 2017-04-16 優瑞科生物技術公司 靶向ｎｙ－ｅｓｏ－１肽／ｍｈｃ複合體之構築體及其用途

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
DK0585287T3 (da) 1990-07-10 2000-04-17 Cambridge Antibody Tech Fremgangsmåde til fremstilling af specifikke bindingsparelementer
EP0605522B1 (en) 1991-09-23 1999-06-23 Medical Research Council Methods for the production of humanized antibodies
PT1024191E (pt) 1991-12-02 2008-12-22 Medical Res Council Produção de auto-anticorpos a partir de reportórios de segmentos de anticorpo e exibidos em fagos
CA2131151A1 (en) 1992-03-24 1994-09-30 Kevin S. Johnson Methods for producing members of specific binding pairs
GB9313509D0 (en) 1993-06-30 1993-08-11 Medical Res Council Chemisynthetic libraries
WO1995015388A1 (en) 1993-12-03 1995-06-08 Medical Research Council Recombinant binding proteins and peptides
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
ATE299938T1 (de) 1997-05-02 2005-08-15 Genentech Inc Ein verfahren zur herstellung multispezifischer antikörper die heteromultimere und gemeinsame komponenten besitzen
DK1071700T3 (da) 1998-04-20 2010-06-07 Glycart Biotechnology Ag Glykosylerings-modifikation af antistoffer til forbedring af antistofafhængig cellulær cytotoksicitet
CA2704600C (en) 1999-04-09 2016-10-25 Kyowa Kirin Co., Ltd. A method for producing antibodies with increased adcc activity
JP2003531588A (ja) 2000-04-11 2003-10-28 ジェネンテック・インコーポレーテッド 多価抗体とその用途
CA2953239A1 (en) 2000-10-06 2002-04-18 Kyowa Hakko Kirin Co., Ltd. Antibody composition-producing cell
JP2003111595A (ja) 2001-06-25 2003-04-15 Kyogo Ito 腫瘍抗原
ATE417065T1 (de) 2004-05-19 2008-12-15 Medigene Ltd Hochaffiner ny-eso-t-zellen-rezeptor
AU2006232287B2 (en) 2005-03-31 2011-10-06 Chugai Seiyaku Kabushiki Kaisha Methods for producing polypeptides by regulating polypeptide association
US7612181B2 (en) 2005-08-19 2009-11-03 Abbott Laboratories Dual variable domain immunoglobulin and uses thereof
JP6157046B2 (ja) 2008-01-07 2017-07-05 アムジェン インコーポレイテッド 静電的ステアリング（ｅｌｅｃｔｒｏｓｔａｔｉｃｓｔｅｅｒｉｎｇ）効果を用いた抗体Ｆｃヘテロ二量体分子を作製するための方法
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
WO2011028952A1 (en) 2009-09-02 2011-03-10 Xencor, Inc. Compositions and methods for simultaneous bivalent and monovalent co-engagement of antigens
HRP20241208T1 (hr) 2010-04-20 2024-11-22 Genmab A/S Heterodimerni proteini koji sadrže fc fragment protutijela i postupci za njihovu proizvodnju
BR112014010580B1 (pt) 2011-11-04 2021-01-12 Zymeworks, Inc. constructo de fc heteromultimérico isolado, composição, uso de um constructo de fc heteromultimérico isolado, composição de ácido nucléico e método para expressar o constructo de fc heteromultimérico isolado
EP2832856A4 (en) 2012-03-29 2016-01-27 Chugai Pharmaceutical Co Ltd ANTI-LAMP5 ANTIBODIES AND USE THEREOF
RU2639287C2 (ru) * 2012-06-27 2017-12-20 Ф. Хоффманн-Ля Рош Аг Способ отбора и получения высокоселективных и мультиспецифичных нацеливающих групп с заданными свойствами, включающих по меньшей мере две различные связывающие группировки, и их применения
KR102211837B1 (ko) 2013-01-14 2021-02-03 젠코어 인코포레이티드 신규한 이형이량체 단백질
KR102332303B1 (ko) 2013-05-31 2021-11-26 자임워크스 인코포레이티드 감소되거나 침묵화된 효과기 기능을 갖는 이종다량체
RU2585091C1 (ru) 2015-01-19 2016-05-27 государственное бюджетное образовательное учреждение высшего профессионального образования "Кемеровская государственная медицинская академия" Министерства здравоохранения Российской Федерации Способ определения антител к аллогенным hla-g
GB201522592D0 (en) 2015-12-22 2016-02-03 Immunocore Ltd T cell receptors
TWI781108B (zh) 2016-07-20 2022-10-21 比利時商健生藥品公司 抗ｇｐｒｃ５ｄ抗體、結合ｇｐｒｃ５ｄ與ｃｄ３之雙特異性抗原結合分子及其用途
JP7202185B2 (ja) 2016-12-22 2023-01-11 第一三共株式会社 抗ｃｄ３抗体及び該抗体を含む分子
JP2020061476A (ja) 2018-10-11 2020-04-16 ソニーセミコンダクタソリューションズ株式会社 固体撮像装置及び電子機器
JP7674278B2 (ja) 2019-07-03 2025-05-09 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 抗ニューヨーク食道扁平上皮がん１（ｎｙ－ｅｓｏ－１）抗原結合タンパク質およびその使用方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010106431A2 (en) * 2009-03-20 2010-09-23 Ludwig Institute For Cancer Research Ltd High affinity t-cell receptor-like ny-eso-1 peptide antibodies, methods, and uses thereof
TW201713701A (zh) * 2015-06-24 2017-04-16 優瑞科生物技術公司 靶向ｎｙ－ｅｓｏ－１肽／ｍｈｃ複合體之構築體及其用途

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
期刊 Gerhard Held et al., "Dissecting cytotoxic T cell responses towards the NY-ESO-1 protein by peptide/MHC-specific antibody fragments" Eur J Immunol., 34(10), Wiley, 2004 Oct, p. 2919-29. *
期刊 Guillaume Stewart-Jones et al., "Rational development of high-affinity T-cell receptor-like antibodies" PNAS, 106(14), NAS, 2009 Apr 7, p. 5784-8.; *

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