TWI827531B - 基於使用增強表現基因座之製備抗體的組成物及方法 - Google Patents

基於使用增強表現基因座之製備抗體的組成物及方法 Download PDF

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TWI827531B
TWI827531B TW106113298A TW106113298A TWI827531B TW I827531 B TWI827531 B TW I827531B TW 106113298 A TW106113298 A TW 106113298A TW 106113298 A TW106113298 A TW 106113298A TW I827531 B TWI827531 B TW I827531B
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TW201803986A (zh
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羅伯特 鮑比
達爾亞 布維克弗
剛 陳
詹姆斯 凡德
趙瑜
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美商再生元醫藥公司
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Biophysics (AREA)
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  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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TW106113298A 2016-04-20 2017-04-20 基於使用增強表現基因座之製備抗體的組成物及方法 TWI827531B (zh)

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US201662325400P 2016-04-20 2016-04-20
US62/325,400 2016-04-20

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TW201803986A TW201803986A (zh) 2018-02-01
TWI827531B true TWI827531B (zh) 2024-01-01

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US (2) US11512144B2 (enExample)
EP (1) EP3445781A1 (enExample)
JP (4) JP7096770B2 (enExample)
KR (3) KR102547738B1 (enExample)
CN (2) CN116515757A (enExample)
AR (1) AR109451A1 (enExample)
AU (2) AU2017253241B2 (enExample)
BR (1) BR112018071283A2 (enExample)
CA (1) CA3015389A1 (enExample)
EA (1) EA201892010A1 (enExample)
IL (2) IL314779A (enExample)
MX (2) MX2018012868A (enExample)
SG (2) SG11201807885PA (enExample)
TW (1) TWI827531B (enExample)
WO (1) WO2017184832A1 (enExample)

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SG11202110076PA (en) * 2019-04-02 2021-10-28 Chugai Pharmaceutical Co Ltd Method of introducing target-specific foreign gene
EP3990649A1 (en) 2019-06-26 2022-05-04 Genentech, Inc. Randomized configuration targeted integration of nucleic acids
AR127674A1 (es) 2021-10-18 2024-02-21 Regeneron Pharma Células de mamífero que comprenden genes cas9 integrados para producir sitios de integración estables, y células de mamífero que comprenden sitios de integración estables y otros sitios
JP2024537896A (ja) 2021-10-18 2024-10-16 リジェネロン・ファーマシューティカルズ・インコーポレイテッド アデノウイルス随伴ウイルスポリヌクレオチドを含む真核細胞
CN118159660A (zh) 2021-10-18 2024-06-07 瑞泽恩制药公司 多核苷酸的受控转录
CN119731330A (zh) * 2021-12-22 2025-03-28 基因泰克公司 多载体重组酶介导的盒式交换
CN118974078A (zh) * 2022-02-04 2024-11-15 小利兰·斯坦福大学董事会 替代细胞因子多肽
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CN121794393A (zh) 2023-09-07 2026-04-03 里珍纳龙药品有限公司 经共价表面修饰的腺相关病毒的生产和纯化
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WO2025115876A1 (ja) 2023-11-29 2025-06-05 富士フイルム株式会社 細胞の作出方法、細胞及びタンパク質の製造方法
KR20260103112A (ko) 2023-11-29 2026-07-02 후지필름 가부시키가이샤 탐색 방법, 세포의 작출 방법, 세포의 선별 방법, 세포 및 세포 생산물의 제조 방법
WO2026050618A1 (en) 2024-08-29 2026-03-05 Regeneron Pharmaceuticals, Inc. Polynucleotide constructs and cell lines for recombinant aav production

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CN109195986A (zh) 2019-01-11
MX2018012868A (es) 2019-03-11
IL262266A (en) 2018-11-29
TW201803986A (zh) 2018-02-01
US12528883B2 (en) 2026-01-20
IL262266B1 (en) 2024-09-01
EA201892010A1 (ru) 2019-03-29
IL262266B2 (en) 2025-01-01
JP7096770B2 (ja) 2022-07-06
KR20260011194A (ko) 2026-01-22
SG10202010155YA (en) 2020-11-27
EP3445781A1 (en) 2019-02-27
IL314779A (en) 2024-10-01
JP7781128B2 (ja) 2025-12-05
CA3015389A1 (en) 2017-10-26
SG11201807885PA (en) 2018-10-30
WO2017184832A1 (en) 2017-10-26
KR102547738B1 (ko) 2023-06-26
JP2025188169A (ja) 2025-12-25
KR20180134893A (ko) 2018-12-19
AR109451A1 (es) 2018-12-12
US11512144B2 (en) 2022-11-29
TW202415767A (zh) 2024-04-16
JP2021164479A (ja) 2021-10-14
BR112018071283A2 (pt) 2019-02-12
JP2024023449A (ja) 2024-02-21
JP2019515666A (ja) 2019-06-13
KR102906454B1 (ko) 2026-01-02
AU2017253241A1 (en) 2018-09-20
CN109195986B (zh) 2023-01-03
CN116515757A (zh) 2023-08-01
KR20230098361A (ko) 2023-07-03
MX2025003852A (es) 2025-05-02
US20230130799A1 (en) 2023-04-27
US20190233544A1 (en) 2019-08-01
AU2024220124A1 (en) 2024-10-17
AU2017253241B2 (en) 2024-07-04

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