TWI662898B - A synergistic composition comprising insecticides and fungicides - Google Patents

Abstract

The present invention relates to a synergistic composition comprising an insecticidal component (A) containing fenpronil and thiodichloride; and a fungicidal component (B) containing subtomin and methyldoxetrol. The invention also relates to methods for preventing, controlling and / or treating insect, nematode and fungal infections in plants, plant parts and / or the surrounding environment by applying said synergistic composition.

Description

Synergistic composition containing insecticide and fungicide

The present invention relates to a synergistic composition comprising (A) an insecticidal component comprising fipronil and thiodicarb; and (B) comprising one or more selected from azoxystrobin And the fungicidal component of thiophanate-methyl. The invention also relates to methods for preventing, controlling and / or treating insect, nematode and fungal infections in plants, plant parts and / or their surroundings by applying the synergistic composition of the invention at the location to be treated. The present invention is easily related to the use of the aforementioned composition, particularly in the treatment of seeds, leaves and soil applications.

Nematode and insect infections, as well as fungal diseases, are major threats to important economic crops such as cereals, fiber, legumes and sugar cane. Yields of plants such as sugar cane and soybeans are negatively affected by nematode, insect and fungal attack. Therefore, there is a continuing need to provide compositions for preventing, controlling, and / or treating nematode, insect, and fungal infections to increase plant yield.

Researchers in the chemical field have synthesized a variety of chemicals and preparations to deal with nematode, insect, and fungal infections. Different types of insecticides and fungicides are known and available on the market. In some cases, combined application of ingredients that have shown insecticidal and fungicidal activity is more effective than single application, and this is referred to as "synergy". As defined in the seventh edition of the Herbicide Handbook by the Weed Science Society of America (1994, p. 318), "'synergy' [yes] two or more The interaction of these factors makes the effect when combined is greater than the expected effect based on each factor when applied alone. " The invention is based on the finding that certain fungicides and insecticides show synergistic effects when applied in combination.

Insecticide and fungicidal active ingredients forming the synergistic composition of the present invention in terms of plant protection The effects are respectively known in the art. They are all published in the Twelfth Edition (2000) of The Pesticides Manual published by The British Crop Protection Council. They are also commercially available.

Yatomin, a methoxyacrylate type fungicide, is a well-known fungicide with broad spectrum disease control. It is extracted from the fungus Strobilurus tenacellus . It has inhibitory effects on other fungi and slows down competition for nutrients; it inhibits electron transfer between cytochrome b and cytochrome C ₁ at the ubiquitin oxidation site in mitochondria, interferes with metabolism and prevents the growth of target fungi.

Methyldoxetine is a systemic fungicide with protective and therapeutic effects. It is absorbed through leaves and roots.

Fenipni belongs to a class of insecticides known as phenylpyrazoles. Fenipney through the γ-aminobutyric acid (GABA) -regulated chloride channel, hinder the chloride ion pathway and interfere with CNS activity. It is moderately absorbent and can be used to control insects when applied as a soil or seed treatment. WO2013037291 A1 discloses a method for preparing fenpnyl.

Thiodicarb is an N-methyl oxyimidothioate carbamate insecticide. It is a neurotoxic compound and its mode of action is through inhibition of cholinesterase.

The present invention is directed to a synergistic composition comprising: (A) an insecticidal component comprising fenpronil and thiodichloride; and (B) a fungicidal component comprising subtomin and / or methyl polysulfide.

The synergistic composition of the present invention may provide benefits over the use of components (A) and (B) alone. This can significantly reduce the rate of application of the individual components while maintaining a high level of insecticidal or fungicidal efficacy. The composition can have a fairly broad-spectrum effect, which is more effective in this respect than any individual component. The composition may have the potential to control fungal and / or insect and / or nematode species at low application rates, while individual compounds are ineffective when applied alone at such low application rates. The rate of action of the composition can be faster than the expected rate of the individual components.

The composition comprises an insecticidally effective amount of component (A) and a fungicidal effective amount of component (B).

The term "effective amount" refers to the amount of said compound or combination of said compounds capable of providing the prevention, control and / or treatment of insect, nematode and / or fungal infections of plants.

"Plant" as used herein refers to all plants and plant populations, such as desired and unwanted wild plants or crops.

As used herein, "plant part" refers to all parts and organs of a plant, such as shoots, leaves, needles, stems, stems, fruiting bodies, fruits, seeds, roots, tubers and rhizomes. Also included are harvested materials, as well as asexual and sexually propagating materials, such as cuttings, tubers, meristems, rhizomes, side branches, seeds, single plant cells and multiple plant cells, and any other plant tissue.

The term "environment" or "location" refers to a place where the plant is grown, a place where the plant propagation material of the plant is sown, or a place where the plant propagation material of the plant is to be sown.

The invention also relates to a method for preventing, controlling and / or treating insect, nematode and fungal infections, and to the use of said synergistic composition.

It has also been surprisingly discovered that when plants, plant parts and / or their surroundings (especially cereals, fibrous plants, leguminous plants and sugar cane) are applied with an insecticidal component containing (A) containing fenpnyl and thiodichlor; With (B) synergistic compositions containing subtomic and / or methyl polysecure fungicidal components, superior performance in preventing, controlling and / or treating insect, nematode and fungal infections will be observed.

The insecticidal component (A) may be present in the composition of the present invention in any suitable amount, and it is usually present in an amount of about 20% to about 80% (by weight of the composition), preferably about 30% to about 70% (by weight of the composition), more preferably about 35% to about 65% (by weight) or about 40% to about 65% (by weight of the composition).

Fenipney may be present in the composition of the present invention in any suitable amount, and is generally present in an amount of about 1% to about 50% (by weight of the composition), preferably about 1% to about 30. % Or about 5% to about 20% (by weight of the composition), more preferably about 5% to about 15% (by weight of the composition). In some embodiments, fenipni is present in an amount of about 10% (by weight of the composition). In certain embodiments, fenipni is present in an amount of about 7% (by weight of the composition).

Thiodicarb may be present in the composition of the present invention in any suitable amount, and is generally present in an amount of about 1% to about 70% (by weight of the composition), preferably about 1% to about 55. % Or about 30% to about 55% (by weight of the composition), more preferably about 32% to about 50% (by weight of the composition). In some embodiments, thiodione is present in an amount of about 44% (by weight of the composition). In certain embodiments, thiodione is present in an amount of about 35% (by weight of the composition).

The fungicidal component (B) may be present in the composition of the invention in any suitable amount, and generally It is present in an amount of about 0.5% to about 50% (by weight of the composition), preferably about 0.5% to about 30% or about 0.5% to about 20% (by weight of the composition) , More preferably about 0.5% to about 15% (by weight of the composition).

Yatomin may be present in the composition of the present invention in any suitable amount, and is generally present in an amount of about 0% to about 50% (by weight of the composition), preferably about 0.5% to about 30. % Or about 0.5% to about 15% (by weight of the composition), more preferably about 0.5% to about 10% (by weight of the composition). In some embodiments, yatomin is present in an amount of about 6% (by weight of the composition). In certain embodiments, attomin is present in an amount of about 0.6% (by weight of the composition).

Methyl polysulfide may be present in the composition of the invention in any suitable amount, and is generally present in the following amount: about 0% to about 50% (by weight of the composition), preferably about 0.5% to About 30% or about 0.5% to about 15% (by weight of the composition), more preferably about 5% to about 10% (by weight of the composition). In some embodiments, the methyl polysulfide is present in an amount of about 7.4% (by weight of the composition).

In some embodiments, the weight percentages of the components in the composition are independently: about 5% to about 12% of fenproni; about 40% to about 50% of thiodiphene; and about 5% to about About 10% of atormin (by weight of the composition).

In certain embodiments, the weight percentages of the components in the composition are independently: about 5% to about 10% of fenproni; about 30% to about 40% of thiodiphene; about 0.5% to About 1% of yatomin; and about 5% to about 10% of methyl doxorubicin (by weight of the composition).

Components (A) and (B) may be present in the composition or applied in any amount relative to each other to provide an enhancement or synergy of the mixture. Specifically, the weight ratio of components (A) and (B) in the composition is independently preferably about 50: 1 to about 1:50 or about 15: 1 to about 1:15, more preferably about 12: 1 to about 1:12, and even more preferably about 9: 1 to about 1: 9. In some embodiments, the weight ratio of components (A) and (B) in the composition is about 9: 1, or 8: 1 or 7: 1 or 6: 1 or 5: 1 or 4: 1 Or 3: 1 or 2: 1 or 1: 1. In some embodiments, in terms of seed treatment, the weight ratio of components (A) to (B) in the composition is about 5: 1.

Components (A) and (B) may be present in the composition together in any suitable amount, and are generally present in an amount of from about 2% to about 95% (by weight of the composition), compared to It is preferably about 25% to about 80% (by weight of the composition), more preferably about 35% to about 70%, and even more preferably about 45% to about 65% (by weight of the composition).

In a preferred embodiment of the invention, each combination is a composition comprising components (A) and (B), and optionally one or more auxiliaries. The adjuvant used in the composition will depend on the type of formulation and / or the way the end user applies the formulation. The formulations incorporating the composition of the present invention are described later. Suitable adjuvants that can be included in the composition of the present invention are all conventional formulation adjuvants or components, such as extenders, carriers, solvents, surfactants, stabilizers, defoamers, antifreeze agents , Preservatives, antioxidants, colorants, thickeners, solid adhesives and inert fillers. Suitable auxiliaries are known in the art and are commercially available. Its use in the formulation of the composition according to the invention will be apparent to those skilled in the art.

The composition also includes one or more inert fillers. The inert fillers are known in the art and are commercially available. Suitable fillers in solid form are, for example, natural ground minerals such as kaolin, alumina, talc, chalk, quartz, attapulgite, montmorillonite, and diatomaceous earth, or synthetic ground fines Minerals, such as highly dispersed silicic acid, alumina, silicates, and calcium phosphate, and calcium hydrogen phosphate. Suitable inert fillers for granules include, for example, crushed and fractionated natural minerals such as calcite, marble, pumice, sepiolite, and dolomite, or particles of synthetic inorganic and organic ground materials, and organic materials (Such as sawdust, coconut husks, corn cobs, and tobacco stalks).

The composition optionally comprises one or more surfactants, which are preferably nonionic, cationic and / or anionic in their natural state, and a mixture of surfactants having good emulsifying, dispersing and wetting properties. This depends on the nature of the active compound to be configured. Suitable surfactants are known in the art and are commercially available. Suitable anionic surfactants can be referred to as water-soluble soaps and water-soluble synthetic surface-active compounds. Useful soaps are alkali metal, alkaline earth metal or ammonium salts of substituted or unsubstituted higher fatty acids (C ₁₀ -C ₂₂ ), such as sodium or potassium salts of oleic acid or stearic acid or a mixture of natural fatty acids. The surfactant may be an ionic or non-ionic emulsifier, dispersant or wetting agent. Useful examples are salts of polyacrylic acid, salts of lignosulfonic acid, salts of phenylsulfonic acid or naphthalenesulfonic acid, polycondensates of ethylene oxide with fatty alcohols or with fatty acids or with fatty amines, substituted phenols ( Especially alkylphenols), salts of sulfosuccinates, taurine derivatives (especially alkyltaurates), or phosphates of polyethoxyphenols or alcohols. When the active compound and / or inert carrier and / or adjuvant / adjuvant is insoluble in water and the carrier used for the final application of the composition is water, it is generally required that at least one surfactant is present.

The composition optionally further comprises one or more polymer stabilizers. Suitable polymer stabilizers useful in the present invention include, but are not limited to, polypropylene, polyisobutylene, polyisopentanediol, Copolymers of olefins and diolefins, polyacrylates, polystyrene, polyvinyl acetate, polyurethanes or polyamides. Suitable stabilizers are known in the art and are commercially available.

In general, the surfactants and polymer stabilizers described above are believed to provide stability to the composition, allowing the composition to be formulated, stored, transported, and applied.

Suitable defoamers include all substances conventionally used in agrochemicals for this purpose. Suitable defoamers are known in the art and are commercially available. A particularly preferred defoamer is a mixture of polydimethylsiloxane and perfluoroalkylphosphonic acid, such as silicone defoamers available from GE or Compoton.

Suitable organic solvents are selected from all common organic solvents capable of completely dissolving the active compound used. Likewise, suitable organic solvents for the active components (A) and (B) are known in the art. The following may be considered as preferred materials: N-methylpyrrolidone, N-octylpyrrolidone, cyclohexyl-1-pyrrolidone; or SOLVESSO ^TM 200, a mixture of paraffins, isoparaffins, cycloparaffins and aromatics. Suitable solvents are commercially available.

Suitable preservatives include all substances conventionally used in this type of agrochemical composition to achieve this purpose, and are also well known in the art. Suitable examples may include include PREVENTOL ^® (from Bayer (Bayer AG)), and PROXEL ^® (from Bayer).

Suitable antioxidants are all substances conventionally used in agrochemical compositions to achieve this purpose, and are known in the art. Butylated hydroxytoluene is preferred.

Suitable thickeners include all substances which can be conventionally used in agrochemical compositions to achieve this purpose. Examples include xanthan gum, PVOH, cellulose and its derivatives, clay hydrated silicates, magnesium aluminosilicates, or mixtures thereof. As such, such thickeners are known in the art and are commercially available.

The composition also includes one or more solid adhesives. Such adhesives are known in the art and are commercially available. It includes organic adhesives, including tackifiers, such as cellulose substituted cellulose, natural and synthetic polymers, in the form of powders, granules, or meshes, and inorganic adhesives such as gypsum, silica, or cement.

In addition, depending on the formulation, the composition of the present invention may further include water.

In some embodiments, the composition of the present invention comprises a combination of: (A) fenpronil and thiodigram; and (B) yatomin; (A) fenpronil and thiodigram; and ( B) methyl poly net; and (A) fenproni and thiodimeth; and (B) subtomin and methyl poly net.

Each of the compositions of the present invention is useful in agricultural areas and relates to controlling insects (including termites, Beetles, moths, weevil families), polypods, nematodes and fungi, such as but not limited to: wheat: nematodes: Meloidogyne javanica; Pratylenchus brachyurus; ants : Acromyrmex landolti landolti, Atta capiguara, Atta sexdens rubropilosa beetle: Migdolus fryanus; Tucuman chafer (Diloboderus abderus) termites: Cornitermes cumulans; Heterotermes tenuis; Neocapritermes opacus; Procornitermes triacifer fungi: Bipolaris oryzae ), Bipolaris sorokiniana, Drechslera teres, Drechslera tritici-repentis, Puccinia coronata var.avenae), Puccinia triticina, Pyricularia grisea moth: Elasmopalpus lignosellus; Spodoptera frug iperda); Agrotis ipsilon; Diatraea saccharalis; cotton: nematode: Meloidogyne javanica; Pratylenchus brachyurus; moth: corn seed spot of South America Stingray (Elasmopalpus lignosellus); Spodoptera frugiperda; Agrotis ipsilon; Heliothis virescens

Ants: Acromyrmex landolti landolti, Atta sexdens rubropilosa

Beetle: Cotton bell weevil (Anthonomus grandis)

Fungi: Ramularia areola

Thrips: Schulch flower thrips (Frankliniella schultzei)

Worms: Alabama argillacea

sugar cane:

Nematodes: Meloidogyne javanica; Pratylenchus brachyurus; Termites: Heterotermes tenuis; Cornitermes cumulan; Beetles: Migdolus fryanus; Sphenophorus levi; Fungi: Pineapple disease (fungi complex, The main ones are: Ceratocystis paradoxa); Rotten Roots (Pythium spp); Cuttings Disease (Phytophthora spp.); And Fusarium Disease (Semi-Nude) Fusarium semitectum)

Soybean: Nematodes: Meloidogyne javanica; Pratylenchus brachyurus; Beetles: Phyllophaga cuyabana; Diabrotica speciosa; Megascelis aeruginosa ; Moths: Elasmopalpus lignosellus; Spodoptera frugiperda; Agrotis ipsilon; Weevil: Sternechus subsignatus; Polypods: Julus Hesperus); fungi: purple spot disease (Cercospora kikuchii); stem anthracnose (Colletotrichum dematium f. Sp. Truncatum); Fusarium sp (Fusarium semitectum)); black spots, stem ulcers, rotten species (Diaporthe phaseolorum var.sojae); stem rot (Sclerotinia sclerotiorum); catastrophic disease, cotton rot ( Pythium spp.); Cutting flower disease (Phytophthora spp.)

The composition of the present invention is suitable for crop plants: cereals (wheat, barley, rye, oats, corn, rice, sorghum, triticale and related crops); beets (such as sugar beets and fodder beets); fruits, such as pears, Stone fruits and small fruits, such as apples, grapes, pears, plums, peaches, apricots, cherries, and berries, such as strawberries, raspberries, and blackberries; legumes (dried beans, lentils, peas, soybeans); oil production Plants (rapeseed, mustard, sunflower); Cucurbitaceae (cucurbit, cucumber, melon); fiber plants (cotton, flax, hemp, jute); citrus, such as orange, lemon, grapefruit and citrus; vegetables (spinach, lettuce, Asparagus, kale, carrot, onion, tomato, potato, red pepper); coffee; sugar cane; and ornamentals (flowers, such as roses, shrubs, broad-leaved trees, and evergreens, such as conifers). In certain embodiments, the composition of the invention is applied to cereals, fiber plants, legumes, and sugar cane. In some embodiments, a composition of the invention is applied Used on wheat, soybeans, cotton and sugar cane.

A synergistic composition comprising (A) an insecticidal component comprising fenproni and thiodione; and (B) a fungicidal component comprising subtomin and / or methyl polysulfide are as described above in wheat, soybean , Cotton and sugarcane, their plant parts and / or the surrounding environment are particularly effective in preventing, controlling and / or treating insect, nematode and fungal infections.

The application (application) rate of the composition of the present invention may vary according to factors such as the degree of control required, the type of application, the type of crop, the specific active compound in the combination, and the type of plant, but it is necessary to make the The compound is present in an effective amount that provides the desired effect, such as insect, nematode or fungal control. The application rate of the composition used under specific conditions can be easily determined through experiments. Generally speaking, the composition of the present invention can be about 0.1 kg / ha (kg / ha) to about 4 kg / ha (based on the total amount of active ingredients (component (A) + component (B) in the composition) Application rate). An application rate of about 1 kg of active ingredient (a.i.) / Ha to 1.5 kg of a.i./ha is preferred. For seed treatment, the application rate may be about 10 g ai / ha to about 500 g ai / ha, preferably about 150 g ai / ha to about 250 g ai / ha, based on the active ingredient (component (component ( A) + total amount of component (B)).

The composition of the present invention can be used as an insecticide, a nematicide and a fungicide, which shows a synergistic activity for preventing, controlling and / or treating insect, nematode and fungal infections. The composition can be formulated in the same manner as insecticides, nematicides, and fungicides. The compounds may be administered alone or as part of a two-part system, a three-part, or a four-part system. Components (A) and (B) or active ingredients can be administered in any desired order, in any combination, continuously or simultaneously. In the case where the components (A) and (B) or the active ingredient are applied simultaneously in the present invention, they can be applied as a composition containing the components (A) and (B) or the active ingredient, in which case the group Points (A) and (B) or active ingredients can be obtained from separate formulation sources and optionally mixed with other pesticides (known as tank-mix, ready-to-use, soup-spray or slurry type) ), Or components (A) and (B) or active ingredients are available as a single formulation mixture source (referred to as premixed, concentrated, formulated compounds (or products)) and optionally mixed with other pesticides.

The composition of the present invention can be applied at various concentrations in a variety of ways known to those skilled in the art. The composition can be used to prevent, control and / or treat insect, nematode and fungal infections of plants, plant parts and / or surroundings, as needed, before or after emergence, to plants, plant parts and / or surroundings.

The synergistic composition of the present invention preferably further comprises an agriculturally acceptable carrier. In practice, The composition is industrialized to promote dispersion. For any given compound, the choice of formulation and mode of administration can affect its activity, so the choice should be made based on the actual situation. Therefore, the composition of the present invention can be formulated as: water soluble solution (SL), emulsion (EC), water-based emulsion (EW), microemulsion (ME), water suspension (SC), water-dispersible oil suspension (OD), suspended seed coating (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water-soluble powders (SP), granules (GR), capsules Granules (CG), Fine Granules (FG), Large Granules (GG), Concentrated Emulsions (SE), Microencapsulated Suspensions (CS), and Granules (MG), or preferably Water Suspension (SC) And suspension seed coating (FS).

With such formulations, the plants, plant parts and / or the surrounding environment can be treated and protected against insects, nematodes and Fungus. In general, the formulation can be diluted with water at a ratio of about 100 mL-5L of the composition in 100 L of water. In some embodiments, the composition is formulated as a suspension. For SC formulations, the composition can be diluted with water at a ratio of 2L of the composition in 100L of water. For FS formulations, the composition can be diluted with water at a concentration of 360 mL of the composition in 100 L of water.

The composition can be applied by methods known in the art. The method includes coating, spraying, dipping, soaking, injection, irrigation, and the like.

In addition, other biocidal active ingredients or compositions can be combined with the synergistic composition of the present invention. For example, the composition may contain, in addition to components (A) and (B), herbicides, insecticides, fungicides, bactericides, acaricides or nematicides to expand the spectrum of activity. The composition of the present invention has the following outstanding features; it has a particularly good plant tolerance and is environmentally friendly.

Those skilled in the art will understand that in insect, nematode and fungal tests, there are a number of factors that are not easily controlled that can affect the results of a single test and make it non-repeatable. For example, the results may depend on environmental factors, such as the amount of sunlight and water, soil type, soil pH, temperature, and humidity. Furthermore, the depth of planting, the rate of application of individual and combined insecticides and fungicides, the ratio of each insecticide and fungicide, and the nature of the crop or weed being tested can all affect the test results. Among crop varieties, results may differ from crop to crop.

Embodiments of the invention are provided by way of illustration, and not limitation.

Formulation examples

Example 1-10% Fenipney + 44% Thiodicarb SC

Example 2-15% Fenipney + 25% Thiodicarb SC

Example 3-7% Fenipney + 35% Sulfate FS

Example 4-6% Yatomin SC

Example 5-0.6% attomin + 7.4% methyl poly net FS

Example 6-15% methyl poly net SC

Example 7-10% Fenipney + 44% Thiodicarb + 6% Yatomin SC

Example 8-15% Fenipney + 25% Thiodicarb + 15% Methyl Polycarbonate SC

Example 9-5% Fenipney + 55% Thiodicarb + 1% Atomin + 2.5% Methyl Polysulfide SC

Example 10-7% Fenipney + 35% Thiodicarb + 0.6% Yatomin + 7.4% Methyl Poly-Clean FS

Biological Examples

Field Test 1-Soybean-Soybean Purple Spot Fungus ( CERCOSPORA KIKUCHII ) (Fungus)

Soybean seeds were treated with formulation examples 3, 5 and 10, then sprayed with a conidia suspension of P. sojae, and incubated at 20 ° C and 100% relative atmospheric humidity for 48 hours. Planting the soybean seeds. It was kept in a greenhouse at 15 ° C and 80% relative atmospheric humidity for 15 days. Assess the severity of young soybean plants (Table 1).

Field Test 2-Soybean-Black Line Anthracnose Variety Maple Ingot ( COLLETOTRICHUM DEMATIUM F.SP.TRUNCATUM )

Soybean seeds were treated with formulation examples 3, 5 and 10, and then sprayed with a coniferous suspension of a maple variant of Ingot of Anthracnose, and incubated at 20 ° C and 100% relative atmospheric humidity for 48 hours. Planting the soybean seeds. It was kept in a greenhouse at 15 ° C and 80% relative atmospheric humidity for 15 days. Assess the severity of young soybean plants (Table 2).

Field Test 3- Soybean- Javanese Root-Knot Nematode ( MELOIDOGYNE JAVANICA ) -Nematode

The nematode inoculum was prepared from a pure subpopulation of Meloidogyne javanica from tomato ( Solanum lycopersicom L. ) in a clay container in a greenhouse. This subgroup is based on the morphological characteristics of the perineal surface sample prepared by TAYLOR and NETSCHER (1974), based on the morphology of the male mouth area (EISENBACK et al., 1981), and based on traditional vertical electrophoresis using the techniques of ESBENSHADE and TRIANTAPHYLLOU (1990) The system, the isozyme phenotype obtained by Mini Protean II of BIO-RAD, was previously identified.

A suspension containing eggs and a second stage juvenile larva (J2) was prepared from the root of the tomato. 10 mL of the suspension was inoculated with eggplant for 22 days. After this, the eggplants were transplanted into pots and kept in the greenhouse. After 100 days, the roots of the eggplant were washed and crushed in a mixer with a 0.5% sodium hypochlorite solution. The suspension was then passed through a 200 mesh (0.074 mm opening) screen on a 500 (0.025 mm opening). The eggs and juvenile larvae retained on a 500 mesh screen were collected and washed.

Soy seeds were treated with formulation examples 3, 5 and 10. The seeds were then inoculated with a 3 mL suspension containing 3,000 eggs and a second-stage juvenile Javan root-knot nematode.

The number of galls on 10 grams of roots of soybean plants was counted 50 days after sowing. The results are listed in Table 3 below.

Field Test 4-Soybean- Bradychus Nematode ( PRATYLENCHUS BRACHYURUS ) -Nematode

The nematode inoculum was prepared from a pure subpopulation of Pratylenchus Brachyurus , which was recovered from a sugarcane crop. This pure subpopulation is propagated by crop plants in a clay container in a greenhouse. This subgroup was previously identified based on the morphological characteristics of adult females fixed in temporary loadings, using a dicotomic key created by SANTOS et al. (2005).

Soy seeds were treated with formulation examples 3, 5 and 10. Then, the seed is A 3 mL suspension of B. brevis at the same developmental stage was inoculated, after which the roots were covered with soil.

The number of nematode eggs in the roots of the plants was counted 100 days after sowing. The results are shown in Table 4 below.

Field Test 5-Soybean-Cuyabana Gill Beetle ( PHYLLOPHAGA CUYABANA ) (Beetle)

Soybean seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Beetles ( Phyllophaga cuyabana ) are cultivated in the laboratory. The number of beetles is counted, collected, and placed in the planting area. The remaining beetle population was tested 10 days after sowing. (table 5)

6- soybean field tests - South American Maize seedlings Oligochroa (ELASMOPALPUS LIGNOSELLUS) (Pyralidae)

Soybean seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Moths (larvae) ( Elasmopalpus lignosellus ) are cultivated in the laboratory. The number of larvae was counted, collected, and placed in the planting area. The remaining larval population was tested 15 days after sowing. (Table 6)

Table 6.

Field Test 7-Soybean-Spodoptera Frugiperda (Moth)

Moths (larvae) ( Spodoptera frugiperda ) are cultivated in the laboratory. Larvae were counted, collected, and applied to healthy young soybean plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining larval populations were tested. (Table 7)

Field Test 8-Soybean-Soybean Stem Weevil ( STERNECHUS SUBSIGNATUS ) -Weevil

Elephant beetles ( Sternechus subsignatus ) are cultivated in the laboratory. The number of subject beetles was counted, collected, and then applied to healthy young soybean plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining weevil beetle populations were tested. (Table 8)

Field Test 9-Soybean- JULUS HESPERUS-Polypods

Soybean seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Polypods ( Julus Hesperus ) are cultivated in the laboratory. The number of polypods is counted, collected, and placed in the planting area. The remaining polypod population was tested 10 days after sowing. (Table 9)

Field Test 10-Soybean- Fusillariasis ( FUSARIUM SEMITECTUM )

Juvenile soybean plant formulation examples 1, 2, 4, 6-9 were treated, then sprayed with a conidial suspension of Fusarium semitectum , and incubated at 20 ° C and 100% relative atmospheric humidity for 48 hours. After 15 days in a greenhouse at 15 ° C and 80% relative atmospheric humidity. Assess the severity of young soybean plants (Table 10).

Field Test 11-Soybean-Stem Rot ( SCLEROTINIA SCLEROTIORUM )

Juvenile soybean plant preparation examples 1, 2, 4, 6-9 were treated, then sprayed with a conidia suspension of Sclerotinia sclerotiorum , and incubated at 20 ° C and 100% relative atmospheric humidity for 48 hours. After 15 days in a greenhouse at 15 ° C and 80% relative atmospheric humidity. Assess the severity of young soybean plants (Table 11).

Field Test 12-Soybean-Cotton Rot (Pythium spp .)

Juvenile soybean plant formulation examples 1, 2, 4, 6-9 were treated, then sprayed with a conidial suspension of Pythium spp. , And incubated at 20 ° C and 100% relative atmospheric humidity for 48 hours. After 15 days in a greenhouse at 15 ° C and 80% relative atmospheric humidity. Assess the severity of young soybean plants (Table 12).

Field test 13-soybean-cutting flower blight ( PHYTOPHTHORA SPP.)

Juvenile soybean plant preparation examples 1, 2, 4, 6-9 were treated, then sprayed with a conidial suspension of Phytophthora spp. , And incubated at 20 ° C and 100% relative atmospheric humidity for 48 hours. After 15 days in a greenhouse at 15 ° C and 80% relative atmospheric humidity. Assess the severity of young soybean plants (Table 13).

Field Test 14- Sugarcane- Javanese Root- knot Nematode ( MELOIDOGYNE JAVANICA ) -Nematode

The nematode inoculum was prepared from a pure subpopulation of Javan root-knot nematode ( Meloidogyne javanica ) of a soybean plant (Glycine max L.) kept in a clay container in a greenhouse. This subgroup is based on the morphological characteristics of the perineal surface sample prepared by TAYLOR and NETSCHER (1974), the morphology of the male mouth area (EISENBACK et al., 1981), and based on the traditional vertical electrophoresis using the techniques of ESBENSHADE and TRIANTAPHYLLOU (1990) The system, the isozyme phenotype obtained by Mini Protean II of BIO-RAD, was previously identified.

3 mL samples of Formulation Examples 1, 2, 4, 6-9 were uniformly applied on the soil and near the roots at the ratios shown in the table. After this, the roots of the sugarcane plant were inoculated with a 10 mL suspension containing 3,000 eggs (Javanese root-knot nematodes) and second-stage juvenile Javan root-knot nematodes, after which the roots were covered with soil.

Java root-knot nematodes at different developmental stages in 10 grams of roots were counted 100 days after application. The results are shown in Table 14 below.

Field test 15-sugar cane-short-tailed nematode ( PRATYLENCHUS BRACHYURUS ) -nematode

The nematode inoculum was prepared from a pure subpopulation of Pratylenchus Brachyurus , which was recovered from a sugarcane crop. This pure subpopulation is propagated by crop plants in a clay container in a greenhouse. This subgroup was previously identified based on the morphological characteristics of adult females fixed in temporary loadings, using a dicotomic key created by SANTOS et al. (2005).

3 mL samples of Formulation Examples 1, 2, 4, 6-9 were evenly applied to the soil and near the roots of the sugarcane plant. Thereafter, the roots of the sugarcane plant were inoculated with a 10 mL suspension of Brevidium brachii at different developmental stages, after which the roots were covered with soil.

The number of Pratylenchus Brachyurus at different developmental stages in a 10 gram root sample of the sugarcane plant was counted after 45 days. The results are shown in Table 15 below.

Field test 16- Sugarcane- Sugarcane termites ( HETEROTERMES TENUIS ) -termites

Termites ( Heterotermes tenuis ) are cultivated in the laboratory. The number of termites was counted, collected, and applied to healthy young sugarcane plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then applied to the soil. The remaining termite colonies were detected in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity for 10 days. (Table 16)

Field test 17- Sugarcane- CORNITERMES CUMULAN -termites

Termites ( Cornitermes cumulan ) are cultivated in the laboratory. The number of termites was counted, collected, and applied to healthy young sugarcane plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then applied to the soil. The remaining termite colonies were detected in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity for 10 days. (Table 17)

Field test 18- Sugarcane- MIGDOLUS FRYANUS (beetle)

Beetles ( Migdolus fryanus ) are cultivated in the laboratory. The number of beetles is counted, collected, and applied to healthy young sugarcane plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then applied to the soil. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining beetle populations were tested. (Table 18)

Field Test 19-Sugar Cane- Chrysanthemum Cleopatra ( SPHENOPHORUS LEVI ) (Beetle)

Beetles ( Sphenophorus Levi ) are cultivated in the laboratory. The number of beetles is counted, collected, and applied to healthy young sugarcane plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then applied to the soil. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining beetle populations were tested. (Table 19)

Field test 20-cane-pineapple disease

The young sugarcane plant preparation examples 1, 2, 4, 6-9 were treated and then sprayed with a conidial suspension of a fungal complex, the main one of which is: singular long beak shell ( Ceratocystis paradoxa ) (Causing pineapple disease), and incubated at 20 ° C and 100% relative atmospheric humidity for 48 hours. After 15 days in a greenhouse at 15 ° C and 80% relative atmospheric humidity. Assess the severity of young sugarcane plants (Table 20).

Field Test 21-Wheat-Java Root Knot Nematode ( MELOIDOGYNE JAVANICA ) -Nematode

The nematode inoculum was prepared from a pure subpopulation of Meloidogyne javanica in tomato ( Solanum lycopersicom L. ) in a clay container in a greenhouse. This subgroup was previously identified based on the morphological characteristics of the perineal surface, the morphology of the mouth region, and the isozyme phenotype of the esterase.

3 mL samples of Formulation Examples 1, 2, 4, 6-9 were uniformly applied on the soil and near the roots at the ratios shown in the table. Thereafter, the wheat roots were inoculated with 10 mL suspensions of Java root-knot nematodes of different developmental stages, after which the roots were covered with soil.

The number of galls on 10 grams of roots was measured 90 days after application. The results are shown in Table 21 below.

Field test 22-wheat-short-tailed nematode ( PRATYLENCHUS BRACHYURUS ) -nematode

The nematode inoculum is prepared from a pure subpopulation of Pratylenchus brachyurus in tomatoes ( Solanum lycopersicom L. ) in a clay container in a greenhouse. This subgroup was previously identified based on the morphological characteristics of the perineal surface, the morphology of the mouth region, and the isozyme phenotype of the esterase.

3 mL samples of Formulation Examples 1, 2, 4, 6-9 were uniformly applied on the soil and near the roots at the ratios shown in the table. After that, the roots of the wheat plant were inoculated with 10 mL suspensions of Brachylena brevis at different developmental stages, after which the roots were covered with soil.

The number of short-tailed nematodes at different developmental stages in the roots of wheat plants was counted 90 days after application. The results are shown in Table 22 below.

Field Test 23-Wheat- Migdolus Frayus -Beetle

Wheat seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Beetles ( Migdolus fryanus ) are cultivated in the laboratory. The number of beetles is counted, collected, and placed in the planting area. The remaining beetle swarms were tested 10 days after sowing. (Table 23)

Field test 24-wheat-sugar cane termites ( TERTERS ) -termites

Termites ( Heterotermes tenuis ) are cultivated in the laboratory. The number of termites was counted, collected, and applied to healthy young wheat plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then applied to the soil. The remaining termite colonies were detected in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity for 10 days. (Table 24)

Field test 25-wheat- spodoptera frugiperda -moth

Moths (larvae) ( Spodoptera frugiperda ) are cultivated in the laboratory. The number of larvae was counted, collected, and applied to healthy young wheat plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining larval populations were tested. (Table 25)

Field Test 26-Cotton-Javanese Nematode ( MELOIDOGYNE JAVANICA ) -Nematode

The nematode inoculum was prepared from a pure subpopulation of Meloidogyne javanica in tomato ( Solanum lycopersicom L. ) in a clay container in a greenhouse. This subgroup was previously identified based on the morphological characteristics of the perineal surface, the morphology of the mouth region, and the isozyme phenotype of the esterase.

3 mL samples of Formulation Examples 1, 2, 4, 6-9 were uniformly applied on the soil and near the roots at the ratios shown in the table. Thereafter, the roots of the cotton were inoculated with a 10 mL suspension of Javan root-knot nematodes of different developmental stages, after which the roots were covered with soil.

The number of galls on 10 grams of roots was measured 90 days after application. The results are shown in Table 26 below.

Field Test 27-Cotton-Short-tailed Nematode ( PRATYLENCHUS BRACHYURUS ) -Nematode

The nematode inoculum was prepared from a pure subpopulation of Pratylenchus Brachyurus , which was recovered from a sugarcane crop. This subpopulation is propagated by crop plants in clay containers in a greenhouse. This subgroup was previously identified based on the morphological characteristics of adult females fixed in temporary loadings using a dicotomic key created by SANTOS et al. (2005).

3 mL samples of Formulation Examples 1, 2, 4, 6-9 were evenly applied to the soil and near the roots of cotton plants. Thereafter, the roots of the cotton plant were inoculated with a 10 mL suspension of Brevidium brachii at different developmental stages, after which the roots were covered with soil.

The number of Pratylenchus brachyurus at different developmental stages in a 10 gram root sample of the cotton plant was counted after 45 days. The results are shown in Table 27 below.

Field Test 28-Cotton-Spodoptera Frugiperda (Moth)

Moths (larvae) ( Spodoptera frugiperda ) are cultivated in the laboratory. The number of larvae was counted, collected, and applied to healthy young cotton plants. A 200 mL formulation example was diluted with water and sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining larval populations were tested. (Table 28)

Wheat Field Test 29- - South American Maize seedlings Oligochroa (ELASMOPALPUS LIGNOSELLUS) (Pyralidae)

Wheat seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Moths (larvae) ( Elasmopalpus lignosellus ) are cultivated in the laboratory. The number of larvae was counted, collected, and placed in the planting area. The remaining larval population was tested 15 days after sowing. (Table 29)

Field test 30-wheat-small ground tiger ( AGROTIS IPSILON ) (moth)

Wheat seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Moths (larvae) ( Agrotis ipsilon ) are cultivated in the laboratory. The number of larvae was counted, collected, and placed in the planting area. The remaining larval population was tested 15 days after sowing. (Table 30)

Table 30.

Field tests 31- Cotton - South American corn seedlings Oligochroa (ELASMOPALPUS LIGNOSELLUS) (Minge)

Cotton seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Moths (larvae) ( Elasmopalpus lignosellus ) are cultivated in the laboratory. The number of larvae was counted, collected, and placed in the planting area. The remaining larval population was tested 15 days after sowing. (Table 31)

Field test 32-cotton-small tiger ( AGROTIS IPSILON ) (moth)

Cotton seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Moths (larvae) ( Agrotis ipsilon ) are cultivated in the laboratory. The number of larvae was counted, collected, and placed in the planting area. The remaining larval population was tested 15 days after sowing. (Table 32)

Field Test 33-Soybean-Small Tiger ( AGROTIS IPSILON ) (Moth)

Soybean seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Moths (larvae) ( Agrotis ipsilon ) are cultivated in the laboratory. The number of larvae was counted, collected, and placed in the planting area. The remaining larval population was tested 15 days after sowing. (Table 33)

Field Test 34-Cotton-Spodoptera Frugiperda (Moth)

Cotton seeds were treated with formulation examples 3, 5 and 10 and subsequently planted. Moths (larvae) ( Spodoptera Frugiperda ) are cultivated in the laboratory. The number of larvae was counted, collected, and placed in the planting area. The remaining larval population was tested 15 days after sowing. (Table 34)

Field test 35-wheat-South American corn seed spotted moth ( ELASMOPALPUS LIGNOSELLUS ) (moth)

Moths (larvae) ( Elasmopalus Lignosellus ) are cultivated in the laboratory. The number of larvae was counted, collected, and applied to healthy young wheat plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining larval populations were tested. (Table 35)

Field test 36-wheat-small ground tiger ( AGROTIS IPSILON ) (moth)

Moths (larvae) ( Agrotis ipsilon ) are cultivated in the laboratory. The number of larvae was counted, collected, and applied to healthy young wheat plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining larval populations were tested. (Table 36)

Field Test 37-Cotton-South American Corn Seedlings ( ELASMOPALPUS LIGNOSELLUS ) (Moth)

Moths (larvae) ( ELASMOPALPUS LIGNOSELLUS ) are cultivated in the laboratory. The number of larvae was counted, collected, and applied to healthy young cotton plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining larval populations were tested. (Table 37)

Field Test 38-Cotton- Ground Tiger ( AGROTIS IPSILON ) (Moth)

Moths (larvae) ( Agrotis ipsilon ) are cultivated in the laboratory. The number of larvae was counted, collected, and applied to healthy young cotton plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining larval populations were tested. (Table 38)

Field Test 39-Soybean- Ground Tiger ( AGROTIS IPSILON ) (Moth)

Moths (larvae) ( Agrotis ipstlon ) are cultivated in the laboratory. Larvae were counted, collected, and applied to healthy young soybean plants. Formulation examples 1, 2, 4, 6-9 were diluted with water and then sprayed onto plants. After 10 days in a greenhouse at 21-25 ° C and 80% relative atmospheric humidity, the remaining larval populations were tested. (Table 39)

All publications, patents, and patent applications cited in this specification are incorporated herein by reference, as if each publication or patent application was specifically and individually incorporated herein by reference. Although the present invention has been described in detail by way of illustration and example for clarification purposes, it is not difficult for a person of ordinary skill in the art to understand based on the teachings of the present invention, and may make it without departing from the concept or scope of the scope of the appended patent application. Certain changes and modifications.

Unless the context requires otherwise, it should be understood that the words "including" and "including" and variations thereof such as "containing" and "having" in the scope of this specification and the appended patent applications include the integers or integers referred to. The group means, but does not mean to exclude any other integer or groups of integers.

Any prior art referenced in this specification should not be considered or taken as an acknowledgement or any form of suggestion that the prior art forms part of general common sense.

Claims (14)

A synergistic composition comprising: (A) an insecticidal component comprising fenproni and thiodione; and (B) a fungicidal component comprising subtomin and / or methyl polysulfide. The synergistic composition according to claim 1, wherein the amount of the insecticidal component (A) is 20% to 80% by weight of the composition. The synergistic composition according to claim 1, wherein the amount of the fungicidal component (B) is 0.5% to 50% by weight of the composition. The synergistic composition according to claim 1, wherein the amount of the fenpronil is 1% to 50% by weight of the composition. The synergistic composition according to claim 1, wherein the amount of thiodione is 1% to 70% by weight of the composition. The synergistic composition according to claim 1, wherein the amount of the subatomin is 0% to 50% by weight of the composition. The synergistic composition according to claim 1, wherein the amount of the methyl polyketone is from 0% to 50% by weight of the composition. The synergistic composition according to any one of claims 1-7, wherein the weight ratio of the components (A) to (B) in the composition ranges from about 50: 1 to about 1:50. The synergistic composition according to any one of claims 1 to 7, further comprising an extender, a carrier, a solvent, a surfactant, a stabilizer, a defoamer, an antifreeze, a preservative, an antioxidant, and a coloring agent. Agents, thickeners, solid adhesives and inert fillers; and / or other insecticides and / or nematicides and / or fungicides. The synergistic composition according to any one of claims 1-7, wherein the synergistic composition is in the form of: a water-soluble solution (SL), an emulsion (EC), a water-based emulsion (EW), a microemulsion ( ME), water suspension (SC), water-dispersible oil suspension (OD), suspended seed coating (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powder (WP ), Water-soluble powder (SP), granules (GR), capsule granules (CG), fine granules (FG), large granules (GG), concentrated suspension emulsion (SE), microcapsule suspension (CS), And granules (MG). A method for preventing, controlling and / or treating insect, nematode and fungal infections comprising applying to a plant, plant part and / or its surroundings a synergistic composition according to any one of claims 1-10. The method of claim 11, wherein the plant is selected from the group consisting of cereals, fiber plants, legumes, and sugar cane. The method according to claim 11 or 12, wherein the infection is: wheat: nematode: Meloidogyne javanica, Pratylenchus brachyurus; ant: Acromyrmex landolti landolti), Atta capiguara, Atta sexdens rubropilosa; beetles: Migdolus fryanus, Diloboderus abderus; termites: test Cornitermes cumulans, Heterotermes tenuis, Neocapritermes opacus, Procornitermes triacifer; fungi: Bipolaris oryzae, wheat root rot Bipolaris sorokiniana, Drechslera teres, Barley mesh, Drechslera tritici-repentis, Puccinia coronata var.avenae, wheat leaves Puccinia triticina, Pyricularia grisea; Moths: Elasmopalpus lignosellus, Spodoptera frugiperda, A grotis ipsilon), Diatraea saccharalis; cotton: nematode: Meloidogyne javanica, Pratylenchus brachyurus; moth: Elasmopalpus lignosellus, grassland Spodoptera frugiperda, Agrotis ipsilon, Heliothis virescens; ants: Acromyrmex landolti landolti, Attasexdens rubropilosa; Beetle: Anthonomus grandis; Fungus: Ramularia areola; Thrips: Frankliniella schultzei; Worm: Alabama argillacea; Sugarcane: Nematodes: Meloidogyne javanica, Pratylenchus brachyurus; termites: Heterotermes tenuis, Cornitermes cumulan; beetles: Migdolus fryanus , Sphenophorus levi; fungus: pineapple disease (fungi complex, the main one is: Ceratocystis paradoxa) Rotten Root (Pythium spp.), Cuttings Disease (Phytophthora spp.), Fusarium Disease (Fusarium semitectum); Soybean: Nematode: Java Meloidogyne javanica, Pratylenchus brachyurus; Beetles: Phyllophaga cuyabana, Diabrotica speciosa, Megascelis aeruginosa; Moth: South America Corn seedling spotted (Elasmopalpus lignosellus), Spodoptera frugiperda, Agrotis ipsilon; Weevil: Sternechus subsignatus; Polypods: Julus Hesperus; Fungus : Purple spot disease (Cercospora kikuchii), Stem anthracnose (Colletotrichum dematium f.sp. Truncatum), Fusarium disease (Fusarium semitectum), Black spot disease, stem ulcers, rotten species (Diaporthe phaseolorum var.sojae), stem rot (Sclerotinia sclerotiorum), stuttering disease, cotton rot (Pythium spp .)), Cutting flowers Disease (Phytophthora (Phytophthora spp.)). A use of the composition according to any one of claims 1 to 10 for the prevention, control and / or treatment of insect, nematode and fungal infections in plants, plant parts and / or their surroundings.