TWI507528B - 測試樣品的多重分析方法 - Google Patents

測試樣品的多重分析方法 Download PDF

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Publication number
TWI507528B
TWI507528B TW096101613A TW96101613A TWI507528B TW I507528 B TWI507528 B TW I507528B TW 096101613 A TW096101613 A TW 096101613A TW 96101613 A TW96101613 A TW 96101613A TW I507528 B TWI507528 B TW I507528B
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Taiwan
Prior art keywords
cell
extract
suitable body
test sample
complex
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TW096101613A
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English (en)
Chinese (zh)
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TW200801203A (en
Inventor
James R Heil
Daniel J Schneider
Daniel T Nieuwlandt
Sheri K Wilcox
Dominic Zichi
Todd Gander
Bruce Eaton
Larry Gold
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Somalogic Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/313—Irradiation, e.g. UV irradiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/101—Homogeneous assay format, e.g. one pot reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
TW096101613A 2006-01-17 2007-01-16 測試樣品的多重分析方法 TWI507528B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US75967506P 2006-01-17 2006-01-17

Publications (2)

Publication Number Publication Date
TW200801203A TW200801203A (en) 2008-01-01
TWI507528B true TWI507528B (zh) 2015-11-11

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Country Link
US (1) US20070166740A1 (enExample)
EP (3) EP2952894B1 (enExample)
JP (3) JP5680827B2 (enExample)
KR (1) KR101149688B1 (enExample)
CN (1) CN101374965B (enExample)
AU (1) AU2007205974A1 (enExample)
CA (1) CA2634987C (enExample)
DK (1) DK1994171T3 (enExample)
ES (3) ES2538121T3 (enExample)
HU (1) HUE025489T2 (enExample)
PL (1) PL1994171T3 (enExample)
PT (1) PT1994171E (enExample)
SI (1) SI1994171T1 (enExample)
TW (1) TWI507528B (enExample)
WO (1) WO2007084886A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI835899B (zh) * 2018-10-25 2024-03-21 日商Ｔｌ基因體科技股份有限公司 血液的前處理方法及血液中的細胞分級方法

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US8143004B2 (en) * 2006-09-27 2012-03-27 National University Corporation Tokyo University Of Agriculture And Technology Method of assaying target substance in sample, aptamer molecule method of constructing the same
US7964356B2 (en) * 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US9068216B2 (en) * 2007-03-22 2015-06-30 Bret T. Barnhizer Methods and devices for rapid detection and identification of live microorganisms by aptamers and/or antibodies immobilized on permeable membranes
AU2015261546B2 (en) * 2007-07-17 2017-09-28 Somalogic Operating Co., Inc. Multiplexed analyses of test samples
CA2696431C (en) * 2007-07-17 2021-01-05 Somalogic, Inc. Method for generating aptamers with improved off-rates
AU2013203374B2 (en) * 2007-07-17 2015-04-09 Somalogic Operating Co., Inc. Method for generating aptamers with improved off-rates
GB0719367D0 (en) 2007-10-03 2007-11-14 Procarta Biosystems Ltd Transcription factor decoys, compositions and methods
GB0906130D0 (en) * 2008-10-03 2009-05-20 Procrata Biosystems Ltd Transcription factor decoys
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
GB201002413D0 (en) 2010-02-12 2010-03-31 Procarta Biosystems Ltd Nucleic acid complexes
WO2011109642A1 (en) * 2010-03-03 2011-09-09 Somalogic, Inc. Aptamers to 4-1bb and their use in treating diseases and disorders
US20110275794A1 (en) 2010-04-12 2011-11-10 Somalogic, Inc. 5-position modified pyrimidines and their use
CN102608091A (zh) * 2012-03-22 2012-07-25 中国科学院广州生物医药与健康研究院 一种快速免标记检测硫离子的方法
CA2868096C (en) 2012-03-28 2019-12-31 Somalogic, Inc. Aptamers to pdgf and vegf and their use in treating pdgf and vegf mediated conditions
RU2666989C2 (ru) * 2012-06-07 2018-09-13 Сомалоджик, Инк. Мультиплексные анализы на основе аптамеров
BR112015004537A2 (pt) * 2012-08-31 2017-07-04 Toray Industries método para detecção de um ácido nucleico alvo por hibridização sanduíche
JP6591392B2 (ja) 2013-03-14 2019-10-16 ソマロジック, インコーポレイテッドSomaLogic, Inc. Ｉｌ−６に結合するアプタマー及びｉｌ−６介在性状態の治療または診断におけるそれらの使用
JP6630273B2 (ja) 2013-09-09 2020-01-15 ソマロジック・インコーポレーテッド 安定性が向上したｐｄｇｆ及びｖｅｇｆアプタマー並びにｐｄｇｆ及びｖｅｇｆ媒介性の疾患及び障害の治療におけるそれらの使用
EP3330277A3 (en) 2013-11-21 2018-09-26 Somalogic, Inc. Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
CN103616423A (zh) * 2013-12-02 2014-03-05 济南大学 一种检测土霉素的竞争型适配体传感器的制备方法及应用
KR101506147B1 (ko) * 2013-12-13 2015-03-26 삼성전자 주식회사 시약 세트, 시료 검사방법, 미세유동장치 및 검사장치
CN116334088A (zh) * 2014-02-18 2023-06-27 私募蛋白质体运营有限公司 用于检测微生物的组合物和方法
US10890581B2 (en) * 2014-05-23 2021-01-12 Firefly Bioworks, Inc. Substrate-mediated reactors for bioassays
NO2718257T3 (enExample) * 2014-05-30 2018-04-14
WO2016145362A2 (en) 2015-03-12 2016-09-15 The Regents Of The University Of Michigan Inhibitors of dek protein and related methods
CN109312346B (zh) 2016-07-01 2024-07-30 私募蛋白质体操作有限公司 包含修饰核苷的寡核苷酸
WO2018102115A1 (en) * 2016-12-01 2018-06-07 Aptitude Medical Systems, Inc. Methods and systems for identifying candidate nucleic acid agent
JP7279633B2 (ja) * 2017-03-29 2023-05-23 東洋紡株式会社 核酸の検出方法
WO2019067415A1 (en) * 2017-09-27 2019-04-04 Bio-Rad Laboratories, Inc. DIGITAL AFFINITY LINK ASSAY
JP7217913B2 (ja) * 2018-02-16 2023-02-06 株式会社日立ハイテクサイエンス プラスチック標準物質の製造方法
SG11202009802WA (en) * 2018-04-03 2020-11-27 The Royal Institution For The Advancement Of Learning/Mcgill Univ Colocalization-by-linkage sandwich assays
WO2019236841A1 (en) * 2018-06-08 2019-12-12 Ultivue, Inc. Multiplexed catalyzed reporter deposition
BR112020026129B1 (pt) * 2018-06-22 2023-10-10 Somalogic Operating Co., Inc Métodos para melhorar o desempenho de ensaios proteômicos multiplex
JP7153494B2 (ja) * 2018-07-27 2022-10-14 シスメックス株式会社 生体粒子の測定方法、非特異シグナルの検出方法、生体粒子測定方法及び生体粒子を検出するための試薬キット
KR102129937B1 (ko) * 2018-07-31 2020-07-03 재단법인 아산사회복지재단 종이기반 핵산검출용 키트 및 pcr 증폭산물을 분석하기 위한 방법
CN110824168B (zh) * 2018-08-10 2023-06-09 天津医科大学 一种多肽探针及其在翻译后修饰的结合蛋白鉴定中的应用
LU101073B1 (en) 2018-12-21 2020-06-24 Luxembourg Inst Science & Tech List Dna aptamers specific of adenovirus types
CN113624724A (zh) * 2020-05-07 2021-11-09 廖世奇 一种适配体分子信标对靶分子的多元检测分析方法
CN111735869A (zh) * 2020-05-29 2020-10-02 中山大学 一种蛋白质的检测试剂及检测方法
CN112180102B (zh) * 2020-09-29 2022-06-14 郑州安图生物工程股份有限公司 花生过敏原特异性IgE定量检测试剂、试剂盒及其应用
AU2022258298A1 (en) 2021-04-13 2023-10-05 Somalogic Operating Co., Inc. Modified nucleosides
CN113122544A (zh) * 2021-04-25 2021-07-16 杭州广科安德生物科技有限公司 一种特异性结合timp1蛋白的核酸适配体及其应用
CN113720894B (zh) * 2021-09-02 2022-07-08 清华大学 一种直接采样电离分析系统及方法、应用
EP4605745A1 (en) * 2022-10-21 2025-08-27 Standard Biotools Canada Inc. Tissue imaging replicas
EP4442282A1 (en) 2023-04-07 2024-10-09 POSTECH Research and Business Development Foundation Preparation of site-specific polypeptide conjugates
WO2025166038A1 (en) * 2024-02-02 2025-08-07 Illumina, Inc. Improved aptamer detection techniques

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same

Family Cites Families (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5599720A (en) * 1982-08-27 1997-02-04 Multilyte Limited Measurement of analyte concentration
GB8314523D0 (en) * 1983-05-25 1983-06-29 Lowe C R Diagnostic device
US4587044A (en) * 1983-09-01 1986-05-06 The Johns Hopkins University Linkage of proteins to nucleic acids
US4737453A (en) * 1984-12-12 1988-04-12 Immunomedics, Inc. Sandwich immunoassay utilizing a separation specific binding substance
US4752566A (en) * 1985-12-17 1988-06-21 Genetics Institute, Inc. Displacement polynucleotide method and reagent complex employing labeled probe polynucleotide
GB8803000D0 (en) * 1988-02-10 1988-03-09 Ekins Roger Philip Determination of ambient concentrations of several analytes
US4978608A (en) * 1987-09-04 1990-12-18 Molecular Devices Corporation DNA detection system
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5763177A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US6344321B1 (en) * 1990-06-11 2002-02-05 Gilead Sciences, Inc. Nucleic acid ligands which bind to hepatocyte growth factor/scatter factor (HGF/SF) or its receptor c-met
ATE318832T1 (de) 1990-06-11 2006-03-15 Gilead Sciences Inc Verfahren zur vervendung von nukleinsäureliganden
US5861254A (en) * 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US5270163A (en) * 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5874218A (en) * 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound in a substance using a nucleic acid ligand
US6001577A (en) 1998-06-08 1999-12-14 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US6346611B1 (en) * 1990-06-11 2002-02-12 Gilead Sciences, Inc. High affinity TGfβ nucleic acid ligands and inhibitors
US5654151A (en) * 1990-06-11 1997-08-05 Nexstar Pharmaceuticals, Inc. High affinity HIV Nucleocapsid nucleic acid ligands
US5705337A (en) 1990-06-11 1998-01-06 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5567588A (en) * 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5688935A (en) * 1990-06-11 1997-11-18 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
AU1435492A (en) * 1991-02-21 1992-09-15 Gilead Sciences, Inc. Aptamer specific for biomolecules and method of making
US5582981A (en) * 1991-08-14 1996-12-10 Gilead Sciences, Inc. Method for identifying an oligonucleotide aptamer specific for a target
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US6007987A (en) * 1993-08-23 1999-12-28 The Trustees Of Boston University Positional sequencing by hybridization
US6458539B1 (en) 1993-09-17 2002-10-01 Somalogic, Inc. Photoselection of nucleic acid ligands
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5658738A (en) * 1994-05-31 1997-08-19 Becton Dickinson And Company Bi-directional oligonucleotides that bind thrombin
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5556752A (en) * 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5908745A (en) * 1996-01-16 1999-06-01 University Of Chicago Use of continuous/contiguous stacking hybridization as a diagnostic tool
US6140098A (en) * 1996-08-30 2000-10-31 Schering Corporation Nucleic acids encoding mammalian proteinases; related reagents
KR100566859B1 (ko) * 1997-01-21 2006-04-03 제너럴 하스피톨 코포레이션 Ｒｎａ-단백질 융합물을 이용한 단백질의 선별
US6037137A (en) * 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
DE69834032T2 (de) * 1997-04-23 2006-12-07 Universität Zürich Verfahren zur erkennung von nucleinsäuremolekülen codierend für (poly)peptide, welche mit zielmolekülen interagieren
US20060057573A1 (en) * 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
EP0995798A1 (en) * 1998-10-24 2000-04-26 Novimmune Sa Transcription factor of MHC class II genes, substances capable of inhibiting this new transcription factor and medical uses of these substances
US7074586B1 (en) * 1999-06-17 2006-07-11 Source Precision Medicine, Inc. Quantitative assay for low abundance molecules
JP3463098B2 (ja) * 1999-10-08 2003-11-05 独立行政法人産業技術総合研究所 モジュレートアプタマー及びこれを用いた標的タンパク質の検出方法
SE516272C2 (sv) * 2000-02-18 2001-12-10 Ulf Landegren Metoder och kit för analytdetektion mha proximitets-probning
US6897015B2 (en) * 2000-03-07 2005-05-24 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of pathogens and biological materials
US20020037506A1 (en) 2000-04-18 2002-03-28 Yun Lin Aptamer based two-site binding assay
US6376190B1 (en) 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
EP1377684A4 (en) * 2001-04-11 2009-03-18 Ptc Therapeutics Inc METHODS FOR IDENTIFYING SMALL-SIZED MOLECULES THAT BIND WITH RNA-SPECIFIC STRUCTURAL PATTERNS
US6942972B2 (en) * 2001-10-24 2005-09-13 Beckman Coulter, Inc. Efficient synthesis of protein-oligonucleotide conjugates
WO2003050276A1 (en) 2001-12-05 2003-06-19 Dow Global Technologies Inc. Method for immobilizing a biologic in a polyurethane-hydrogel composition, a composition prepared from the method, and biomedical applications
JP2005517422A (ja) * 2002-02-19 2005-06-16 シンセリカ・コーポレイション 代替抗体ならびにその調製方法および使用
US7659076B2 (en) * 2002-02-28 2010-02-09 Microsens Biophage Limited Binding of pathological forms of prion proteins
AU2003265769A1 (en) 2002-05-02 2003-11-17 Ciphergen Biosystems, Inc. Biochips with surfaces coated with polysaccharide based hydrogels
US7045366B2 (en) 2003-09-12 2006-05-16 Ciphergen Biosystems, Inc. Photocrosslinked hydrogel blend surface coatings
US20040216174A1 (en) * 2003-03-14 2004-10-28 Siegfried Hekimi Screening assays for targets and drugs useful in treatment and prevention of lipid metabolism disorders
US7229763B2 (en) * 2003-04-07 2007-06-12 Beckman Coulter, Inc. Assay system using labeled oligonucleotides
US7195875B2 (en) * 2003-04-18 2007-03-27 Beckman Coulter, Inc. Oligonucleotide pairs for multiplexed binding assays
JP4357284B2 (ja) 2003-05-15 2009-11-04 トヨタ自動車株式会社 内燃機関の制御装置
JP5275566B2 (ja) * 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
US7329742B2 (en) 2003-09-04 2008-02-12 The Regents Of The University Of California Aptamers and methods for their in vitro selection and uses thereof
US20050250147A1 (en) * 2004-05-10 2005-11-10 Macevicz Stephen C Digital profiling of polynucleotide populations
JP2006258458A (ja) 2005-03-15 2006-09-28 Sumitomo Bakelite Co Ltd 医療材料用高分子化合物及び該高分子化合物を用いたバイオチップ用基板

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI835899B (zh) * 2018-10-25 2024-03-21 日商Ｔｌ基因體科技股份有限公司 血液的前處理方法及血液中的細胞分級方法

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