1378769 六、發明說明： 【發明所屬之技術領域】 本發明是提供-種鱗容器之封σ驗成，主要是對大量培 育必須在錢空間成長至-綠度的種苗（如：各種蘭苗之類 ^培養容器的開口可藉由一具有透光、及與外界保持有適度通透 容器蕙/或覆設透氣抑菌膜，來獲有一良好的供給種苗其無菌 成長空間的培養容器且其内部具有極佳之光線照度、渥度、及溫 度的設計。 【先前技術】 按’目前應用在培育必須在無菌空間成長至一定程度的種苗 .(如:各種的蘭苗之類）其培養瓶⑽（如第一圖），通常是以 較㈣玻璃瓶為代表，在其内部充填人培養液⑽（或 培養基），職放人細種苗⑽，之後，在取—較培養瓶 (10胸口（101)内徑略大的橡皮塞⑽予以套入，而該橡皮塞 (40)其中簡是馨貫穿留有一透氣孔⑽)和填滿棉花以利與外ς 空氣呈相通’作為置放入培養瓶⑽其間的種苗⑽在成長過程 能獲有所綱透氣、及光線騎人，_，亦彻在對外銷隹（如· .國内、，或國外的遠近區域）時能仍保持其成長所需環境狀態。 然，該類似種苗培料境所彻之玻璃培養瓶在操作實務與 對外銷售時過程上，則存有如下弊端： 1.該培養瓶由於是藉_#加4成，㈣在成本上較^ =培養瓶是輯璃_彡出’造絲植人所_種苗作對 外鎖售時，由於呈下寬上窄的培養瓶無法作有效空間的堆叠，使" %. «9 1378769 以上的透賴隙結構，在該容器蓋的上端層面為藉紐來覆設一 不透氣的第-基〒’及沿著第_基材膜上是可塗覆以_層抑殺 菌劑層，再由該抑㈣備上端則在藉熱壓來覆設以具不透氣的 膜’以使容器蓋上端是結合有—封口臈，而該封口膜的 -、-基制在作觀之前是顧縫製程來絲出所須範 圍认及大小_之的多數個微觀_:結構(mi⑽卿伽_) 區域，依此，作為在植入種苗後能藉容器蓋其間的封口 良好的供給種苗其成長盔菌办”的 、木獲有 度爾、繼崎糊輸光線照射 本’明$第二目的’是對内部具無菌 腎出，以利在充填入培養液u培養基 等㈣可直接的覆設，膜，=植〇種苗的培養容器在 容器其上端開口為藉熱壓來覆設一不透氣、=成形則是在培養 -基材蹲上是可塗覆以一層抑殺 、、—基材膜，在該第 則在藉熱壓來覆設以具不透氣的第二:材:由：菌:層上端 而該封口膜的第一、第二膜以構成一封口臈， 成形出所須範圍、及大小ς多數個微觀製程來 .為在植入種苗後能藉培養容器其開口上端所覆1域，依此，作 及溫度。 °4^_光_射度、座度、 —設置，— ^不透氣的第二基材膜⑽，以構成—封口膜⑶而該封口膜⑶ 升:第一基材膜(31、33)在作覆設之前是為藉輾壓製程來成 乂出所須範圍、及大小的多數個微觀卿結構區域(34);依此， 為在植入種田⑹後能藉培養容器⑴其開口（11)上端所覆設的 封:膜⑶來财良好的供給種苗⑹其成長空間的培養容器⑴ 内。崎㈤無紐染空氣對流、足夠光線照射度、Μ度保持、及溫 度控制。 另，種苗成長所需含氧空氣可以自培養容器外之大氣經由本 發^所揭露之具有多數個織間隙結構區域的容H蓋封σ膜與培 養谷态内空氣進行對流互換，具有殺抑菌功能之封口膜將防止外 界大氣内所含細菌的入侵，而僅提供無菌空氣進入培養容器内， 此=善了目前市場上習用玻璃培養瓶結合一有塞有棉花之透氣孔 橡膠瓶塞或塑膠罐結合一塞有棉花之透氣孔罐蓋，此玻璃瓶或塑 膠罐體於充填有營養培養液（或培養基）後於高溫高壓殺菌釜内 進仃殺g料祕’相雜絲氣急速膨服或於冷卻降溫過程 體積收縮作用，急速膨脹常導致往外推縮瓶塞或掀開罐蓋而形成 與外界空氣相通之現象，此易造成黴菌汙染，另者導致瓶内體積 大變化與經由充填棉花透氣孔氣體快速流動，往外與往内空氣流 動過大，空氣中夾帶之汙染黴菌有可能入侵内部空間，再一缺點 為於局溫蒸煮過程中熱蒸氣於流經棉花透氣孔或塑膠罐與罐蓋間 隙處容易因冷凝成水而形成一密閉空間，使得含培養液（或培養 基）與殺菌處理後之塑膠罐與罐蓋組合於冷卻過程中的内部氣體 冷縮而導致塑膠罐體的收縮變型，於收縮變型的同時除了有永久 型變形使用上外觀不良，同時於貯運或種苗置入操於過程，掀開 罐蓋的瞬間過程將目急遽軸外大反壓侧而將外界之徽菌帶 入’相反的，本發日月提供一無菌空間培養容器，轉容器與容器 盘結構上之卡榫緊迫結構更結合熱封於培養容器端口或熱封於容 15盘上方之具有—自動動態透氣調壓用封口膜，於殺菌爸高壓高 溫加熱過程與㈣後冷卻_中均能完全確保培養容器外觀無變 形之虞’如此卡榫緊迫轉與自動讎封σ膜設計提供種苗育種 =各階段，如種苗於她、小、中、大苗於半母瓶與子瓶等於 Γ至培育成長過程，能得有—無外來黴轩擾之錢生長空間， Γ培養容器與容器蓋打開與組合過程間能提供一低氣體擾流功 再者於/里至培月種田令需要較長養成時間過程亦能確保一長 期無菌钱環境’本發财所揭露之多數個微觀_結構區域之 2膜，除了可以於培養容器充填營養培養液（或培養基）後， 直接熱封合於培養容器開口端或熱封合於容器蓋之上端後，經由 培養容器與容n制卡財迫結構做—結合，此-封口膜於0 _殺菌爸之_過程愧自_節營養培養液（或培養基 :溫殺_中刷菌過程瞬間彿騰產生之高溫高麵蒸氣，同時， 收ΖΐίΓ過程中因自然的熱服冷縮作用，内部無菌空氣體積 内部培養液（或培養基），該封口膜能快速動態調節 I衡培養⑼之⑽綠敎对之變形，另—特徵為 Μ開口端或容H蓋上雜封以封明^ &互換’提供一無汙染之逑你無菌培養溫室容器空間。 同時對本發明覆設在培養容器⑴其容器蓋⑵上端的封口膜3 (3)/或直接覆設在培養容器⑴其開口⑻上端的封口膜⑶是可 作至少-層m的形態設置，續有預_供給種苗⑹其成長空 間的培養容器⑴内部更佳所需無_染空氣職、足夠光線照射 度、溼度保持、及溫度控制。 實施例中所述之於第一基材膜(31)上是可塗覆以-層抑殺菌 劑層（32)，塗覆方式可以採用凹版塗佈⑽伽）、凸板 I7刷塗佈(Flexo coating)、噴塗(spray c〇ating)或涵浸塗佈 (dipping coating)，建議採用凹版塗佈。另實施例中之抑菌劑材 料亦可以藉材料混練方式直接混練於第一基材膜⑶)原料配方 中’但因混練材料新配方所需抑_量較多賴祕件趨於複雜 而導致iLk成本較局’所以本發贿建議採用塗佈方式。 $本發明作為大她織與種苗培f卫程必須在無妓間成長 至-定程度的種苗，該培養容器及其容器蓋開口上端結合的封口 膜在使用上，是存有如下優點： 、1.該培養容器是以塑膠材質成形出，因此可大幅降低其製造 成本。 該培養容11在植入所須的種苗及覆設以封口膜後，是有利 於，=培養容II作有效空_相互堆疊起，再加上塑膠製品之 低於破璃製品，貯運作業有操作流程簡化，大幅降低運 輸成本之加分正面效果。 3.在制部植人以種苗的培養容器其上端開口所配合的具有 六ϋΓ卜界保持以適度透氣的容器蓋/或覆設膜’是可使培養 $、好的供給種苗其成長空間的内部所需無紐染空氣對 &、足约光線崎度、时保持、及溫度控制環境。 1378769 4.對開π覆如容II蓋/或直接覆設以封口膜的培養容器在 作為種田的成長工間，是可透過封口膜其間所形成的預設範圍及 大j該夕數個微觀間隙結構區域來確切的杜絕外界細菌侵入，以 避免培養容H内部所植人_苗遭感染。 長期植^種苗Ϊ培養容器在觀於溫室其間作為成更 六鹿、*、了培器具有極佳的保濕魏，若遇到長期 ^塵累積或顿財觀於培觀料如 門 抑殺菌劑層將阻止上述灰靡累籍赤么奴♦ 、上笮間 之㈣w 累積或冷凝路水積累所可能夾帶侵入1378769 VI. Description of the invention: [Technical field to which the invention pertains] The present invention provides a sigma test for a scale container, which is mainly for seedlings that must grow in the money space to a green level (for example, various orchid seedlings) The opening of the culture container can obtain a good culture container for supplying the sterile growth space of the seedling by a light-transmitting container and a moderately permeable container/or a gas-permeable bacteriostatic film. Design with excellent illumination, temperature, and temperature. [Prior Art] According to the 'current application in breeding seedlings that must grow to a certain extent in aseptic space. (eg, various blue seedlings, etc.) (As shown in the first figure), usually represented by a (four) glass bottle, filled with human culture solution (10) (or medium), and placed in a fine seedling (10), after which, in the comparison bottle (10 chest (101) a rubber stopper (10) having a slightly larger inner diameter is nested, and the rubber stopper (40) is succinctly inserted through a venting hole (10) and filled with cotton to facilitate communication with the outside air. (10) Seedlings in between (10) In the process of growing up, you can get some ventilation, and the light rides, _, and also keep the environmental status required for growth when it is exported to foreign countries (such as domestic, or foreign countries). In the process of operation practice and external sales, the glass culture bottle of the similar seedling culture material has the following disadvantages: 1. The culture bottle is borrowed _# plus 40%, (4) in terms of cost ^^ The culture bottle is a glass _ 彡 ' 造 造 造 植 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ a gap structure, in the upper end layer of the container cover, a non-breathable first base layer is disposed on the bottom layer of the container cover, and a layer of the fungicide layer is coated on the film of the base substrate, and then (4) The upper end of the preparation is covered with a gas-tight membrane by heat pressing so that the upper end of the container lid is bonded with a sealing jaw, and the -, - base system of the sealing film is required to be threaded out before the observation The range recognizes the size of the majority of the micro_: structure (mi (10) qing _ _) region, according to which, as a seed can be borrowed after implantation The container is covered with a good seal and the seedlings are raised. The wood is harvested. The wood is covered with light. The light is illuminated by the light. The second purpose is to have a sterile kidney inside, so as to fill it in. The culture medium u medium, etc. (4) can be directly covered, and the culture container of the membrane, the vegetable seedling is opened at the upper end of the container to be airtight by heat pressing, and the forming is on the culture substrate. Coated with a layer of anti-killing, - substrate film, in the first step by heat pressing to cover the second: material that is airtight: by: bacteria: upper end of the layer and the first and second of the sealing film The film is formed into a mouth, and the required range and size are formed into a plurality of micro-processes. After implanting the seedlings, the upper end of the opening of the culture container can be covered with a field, and accordingly, the temperature is made. °4^_ Light _ radiance, latitude, - setting, - a second substrate film (10) that is gas impermeable to form a sealing film (3) and the sealing film (3) is raised: the first substrate film (31, 33) is overlaid In the past, it was for the purpose of borrowing and suppressing the process to create a large number of microscopic structures (34) of the required range and size; After the culture container farming ⑴ ⑹ lend an opening (11) covering an upper end of the closure: good film ⑶ fiscal supplied to the culture vessel ⑹ seed space ⑴ its growth. Saki (5) No ray convection, sufficient light exposure, temperature retention, and temperature control. In addition, the oxygen-containing air required for the growth of the seedlings can be convectively exchanged from the atmosphere outside the culture vessel via the H-sealed sigma membrane having a plurality of woven gap structure regions disclosed in the present invention, and the air in the cultured valley state is convectively exchanged. The sealing membrane of the bacteria function will prevent the invasion of bacteria contained in the outside atmosphere, and only provide sterile air into the culture vessel. This is good for the conventional glass culture flasks on the market combined with a vented rubber stopper with cotton plug or The plastic can is combined with a ventilated can lid which is filled with cotton. The glass bottle or the plastic can is filled with the nutrient broth (or medium) and then placed in a high-temperature and high-pressure sterilizing kettle to kill the glutinous material. The shrinkage effect of the service or the cooling and cooling process, the rapid expansion often leads to the phenomenon of pushing the bottle stopper or opening the can lid to form a communication with the outside air, which is easy to cause mold pollution, and the other causes the volume change in the bottle and the filling through the filling. The venting gas of cotton flows rapidly, and the air flowing outwards and inward is too large. The contaminated mold entrained in the air may invade the internal space. Another disadvantage is the temperature. During the cooking process, the hot steam flows through the cotton vent hole or the gap between the plastic tank and the can lid, and is easily condensed into water to form a closed space, so that the culture liquid (or medium) and the sterilized plastic can and can lid are combined. The internal gas in the cooling process shrinks and causes the shrinkage of the plastic can body. In addition to the permanent deformation, the appearance of the shrinkage is poor, and at the same time, the storage and transportation or the seedlings are placed in the process, and the can lid is opened. The process will urgently bring out the large back pressure side and bring the outside bacteria into the opposite direction. The sun and the moon provide a sterile space culture container, and the rotating container and the container tray structure are combined with the heat sealing structure. The culture container port or the heat-sealed sealing plate with the upper surface of the 15 disk has the automatic dynamic gas pressure regulating sealing film, which can completely ensure the appearance of the culture container without deformation after the sterilization high pressure high temperature heating process and (4) post cooling _榫Equipment and automatic 雠 σ 膜 membrane design to provide seedling breeding = various stages, such as seedlings in her, small, medium, large seedlings Can have - no extra money to grow the space, Γ culture container and container lid open and combination process can provide a low gas turbulence work in the / to the cultivating farms to require a longer cultivation time process It can ensure a long-term sterilized money environment. The membrane of most of the micro-structure areas disclosed in this fortune can be directly heat-sealed to the open end of the culture vessel or hot after filling the culture vessel with the nutrient solution (or medium). After sealing at the upper end of the container lid, the culture container is combined with the n-type card-carrying structure, and the sealing film is _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ In the middle of the brushing process, the high-temperature and high-surface steam generated by Fo Teng, at the same time, the natural heat-shrinking effect in the process of collecting Ζΐ Γ, the internal sterile liquid volume internal culture solution (or medium), the sealing film can quickly and dynamically adjust the balance Cultivate (9) (10) green scorpion deformation, and the other feature is Μ open end or H cover with a seal to seal ^ & interchange 'provide a pollution-free 逑 cultivating greenhouse container space. At the same time, the sealing film 3 (3) coated on the upper end of the container lid (2) of the culture container (1) or the sealing film (3) directly disposed on the upper end of the opening (8) of the culture container (1) can be set at least in the form of layer m, continued There is a pre-supply seedling (6) culture space for the growth space (1). It is better to have no internal air, sufficient light, humidity, and temperature control. The first substrate film (31) described in the embodiment may be coated with a layer of bactericide layer (32), and the coating method may be gravure coating (10) gamma), and convex plate I7 brush coating ( For flexo coating, spray c〇ating or dipping coating, gravure coating is recommended. In another embodiment, the bacteriostatic material can also be directly mixed in the first base film (3) by using a material kneading method, but the new formula of the kneading material is required to be more complicated. The cost of iLk is better than that of the bureau', so this bribe is recommended to use the coating method. The invention is a seedling which must be grown to a certain degree in the innocent period, and the sealing film combined with the upper end of the container lid opening has the following advantages: 1. The culture container is formed of a plastic material, thereby greatly reducing the manufacturing cost thereof. The culture volume 11 is advantageous after implanting the seedlings and covering the sealing film, and the culture medium II is effectively empty _ stacked on each other, and the plastic products are lower than the glass products, and the storage operation is carried out. There is a streamlined operational process that significantly reduces the cost of shipping. 3. In the cultivation department where the seedlings are planted, the upper end opening is matched with a container cover/or a covering film which is kept in a moderately ventilated manner, so that the cultivation space can be cultivated. Internally, there is no need to add air to the air, the light, the time, and the temperature control environment. 1378769 4. The culture container with the π-clad cover II or the direct-sealing membrane is used as a growing room for farming, which is a preset range that can be formed through the sealing film and a micro-gap The structural area is to eliminate the intrusion of external bacteria to avoid the infection of the seedlings inside the culture chamber. The long-term planting nursery culture container has excellent moisturizing Wei in the view of the greenhouse as a more six deer, *, and the cultivation device. If it encounters long-term accumulation of dust or money, it can be used as a disinfectant. The layer will prevent the above-mentioned ashes from accumulating red ♦ ♦, the upper (4) w accumulation or the condensation water accumulation may be entrained intrusion
—’而有效防正汗染進入培養容器内感染種苗。 =,技術之人員所瞭解的’以上所 例而已，並非用以限定本發 ^令货Κ較隹貫轭 發明所揭干之揞袖二Ϊ 專利範圍；凡其它未脫離本 辑揭故射谓完叙較改 令 之申請專利範圍内。 — >飾，今應匕3在下述 S] 12 13.78769 【圖式簡單說明】 第一圖：習用玻璃培養瓶的前視剖面示意 第二圖：本發明培養容器在置入殺菌釜作高溫殺菌的實施示 * 意 第三圖：本發明培養容器在充填入培養液的實施示意 第四圖：本發明培養容器在植入種苗的實施示意 第五圖：本發明培養容器在上端覆設以容器蓋的實施示意 第六圖：本發明培養容器在與容器蓋作旋鎖之前的俯視示意 3 第七圖·本發明培養容器在與容器蓋完成旋鎖的俯視示意 第八圖：本發明培養容器在與容II蓋完錢鎖的的前視示意 第九圖：本發明培養容器在完成容器蓋覆設的外觀示意 【主要元件符號說明】 〔習用〕 (10)培養容器 (101)開口 (20)培養液 (30)種苗 (40)橡皮塞 (401)透氣孔 〔本發明〕 (1)培養容器 (11)開口 (12)扣合凹槽 (2)容器蓋 (21)凸部. (22)透氣孔 (3)封口膜 (31)第一基材膜 (32)抑殺菌劑層 (33)弟二基材膜 (34 )微觀間隙結構區域 (4)殺菌釜 (5)培養液 (6)種苗 13-' And effectively prevent the positive sweat from entering the culture container and infecting the seedlings. =, the above examples of the technical personnel know, not to limit the scope of the patents issued by the 轭 发明 发明 ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; Complete the application within the scope of the patent application. — > Decoration, 今应匕3 in the following S] 12 13.78769 [Simple description of the drawing] First: The front view of the conventional glass culture bottle is shown in the second figure: The culture container of the present invention is placed in the sterilization kettle for high temperature sterilization.实施实施方式Fig. 3 is a schematic view showing the implementation of the culture vessel of the present invention in filling the culture solution. FIG. 4 is a schematic view showing the implementation of the culture vessel of the present invention in the implantation of the seedling. The culture vessel of the present invention is covered with a container at the upper end. The sixth embodiment of the present invention is shown in a top view of the culture container of the present invention before being screwed with the container lid. FIG. 7 is a top view of the culture container of the present invention in a lock-up with the container lid. The front view of the front cover of the container with the volume II is shown in the ninth diagram: the appearance of the container of the present invention in the completion of the container cover [main symbol description] [used] (10) culture container (101) opening (20) ) culture solution (30) seedlings (40) rubber stopper (401) venting holes [invention] (1) culture container (11) opening (12) snap groove (2) container cover (21) convex portion. (22 Ventilation hole (3) sealing film (31) first substrate film (3) 2) Antifungal layer (33) Diji substrate film (34) Microscopic gap structure region (4) Sterilization kettle (5) Culture solution (6) Seedling 13