TW201729825A - Composition for promoting hair growth or preventing hair loss comprising extract of ginseng floral axis - Google Patents

Abstract

The present disclosure discloses a composition for promoting hair growth or preventing hair loss, which contains a ginseng floral axis extract as an active ingredient. The extract may be an extract extracted using water, a C1-C5 alcohol or a C1-C5 alcohol aqueous solution and the composition may be used as a food, pharmaceutical or cosmetic composition. The composition exhibits a superior effect of promoting hair growth or preventing hair loss by promoting proliferation of dermal papilla cells.

Description

Composition for promoting hair growth or preventing hair loss containing ginseng flower shaft extract

The present invention relates to a composition for promoting hair growth or preventing hair loss comprising a ginseng flower shaft extract as an active ingredient.

Currently, only two drugs used to treat hair loss are approved by the US Food and Drug Administration (FDA). They were minoxidil and finasteride, first adopted in 1988. The treatment of hair loss remains a challenge given that no new drugs have been approved in the past 30 years.

However, minoxidil has side effects such as hirsutism when administered orally, and according to reports, it takes about 1-2% of patients to have side effects associated with sexual dysfunction, such as Hypoactive sexual desire disorder, ejaculation disorder, impotence, etc. Therefore, there is a need for a safe and effective substance to promote hair growth and prevent hair loss. [References] [Patent Documents] Korean Patent Registration No. 10-0894029.

[Technical Problem] The inventors of the present invention studied the extract of ginseng flower shaft and found that the ginseng flower shaft extract can effectively promote hair growth or prevent hair loss.

In one form, the present invention is directed to a composition for promoting hair growth or preventing hair loss comprising a ginseng flower shaft extract as an active ingredient.

In another form, the invention is directed to providing a composition that promotes hair growth or prevents hair loss as a food, pharmaceutical or cosmetic composition.

[Technical Solution] In one form, the present invention provides a composition for promoting hair growth or preventing hair loss, which comprises a ginseng flower shaft extract as an active ingredient.

In another form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the extract is an extract extracted with an aqueous solution of water, a C ₁ -C ₅ alcohol or a C ₁ -C ₅ alcohol. .

In another type, the present invention provides a method to promote hair growth or prevent hair loss composition, wherein the aqueous solution of the C ₁ -C ₅ alcohol is 40-90% (vol / vol).

In another form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the C ₁ -C ₅ alcohol is one or more selected from the group consisting of methanol, ethanol, and isopropyl. A group consisting of isopropyl alcohol, n-propyl alcohol, n-butanol, and isobutanol.

In another form, the invention provides a composition for promoting hair growth or preventing hair loss, wherein the extract is extracted using a concentration of 60-80% (vol/vol) ethanol aqueous solution. Extracts.

In another form, the invention provides a composition for promoting hair growth or preventing hair loss, wherein the ginseng flower axis is a ginseng flower axis from which ginseng berries have been removed.

In another form, the invention provides a composition that promotes hair growth or prevents hair loss, wherein the composition promotes proliferation of dermal papilla cells.

In another form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the composition for promoting hair growth or preventing hair loss is a food, pharmaceutical or cosmetic composition.

In another form, the invention provides a composition for promoting hair growth or preventing hair loss, wherein the composition is formulated as a powder, a granule, a tablet, a capsule, One of a suspension, an emulsion, a syrup, an aerosol, a formulation for external application, a suppository, and a sterile injection solution.

[Advantageous Effects] According to one form of the present invention, the composition is effective for preventing hair loss.

According to one form of the invention, the composition is effective to promote hair growth.

According to one form of the invention, the composition is effective to promote proliferation of dermal papilla cells.

In one form, the present invention provides an effect of supplying a food composition which is effective for preventing hair loss or promoting hair growth.

In one form, the present invention provides an effect of supplying a pharmaceutical composition which is effective for preventing hair loss or promoting hair growth.

In one form, the present invention provides an effect of supplying a cosmetic composition which is effective for preventing hair loss or promoting hair growth.

According to one form of the invention, the composition has the advantage of recovering the ginseng flower axis, which is typically discarded during human processing.

In general, ginseng refers to the root of ginseng or "ginseng root." "Ginseng Root" has been used as a medicine or food. Panax ginseng C.A. Meyer belongs to Araliaceae ginseng and has been used in Korea, China, Japan, etc. to prevent disease and prolong life. The efficacy and effects of ginseng have been known to date to prevent hair loss, promote hair growth, anti-cancer activity, regulate immune function, anti-diabetic activity, improve liver function, improve cardiovascular disease, control blood pressure, improve menopausal disorder, and resist stress. And anti-fatigue activity, antioxidant activity, anti-aging activity and the like.

In general, the flower axis of ginseng is not used as a medicine or food. The ginseng flower shaft sprouts in May or June of the fourth year after sowing. Usually removed and discarded with ginseng flowers in June to facilitate efficient growth of ginseng roots.

Ginsenosides are representative physiologically active ingredients of ginseng and are evenly distributed in the aerial parts and underground parts of ginseng. Specifically, it is known that ginseng has different contents of ginsenosides depending on different parts (for example, ginseng root, ginseng leaves, ginseng berries, etc.). Different parts exhibit different effects due to differences in composition. For example, ginseng flower extract is about 23 times more effective than ginseng root extract in preventing and treating diabetes.

The inventors of the present invention evaluated the effect of the often discarded ginseng flower shaft on hair growth, and found that the ginseng flower shaft extract has an effect of preventing hair loss or promoting hair growth and enhancing hair health.

Hereinafter, the present invention will be described in detail.

In one form, the present invention provides a composition for promoting hair growth or preventing hair loss, which comprises a ginseng flower shaft extract as an active ingredient.

In the present invention, "ginseng flower axis" means a shaft portion of a ginseng flower or a flower stem.

In the present invention, "extract" refers to a substance extracted from a natural substance regardless of the extraction method or the extracted component. It is used in a wide range of concepts, including an extract obtained by dissolving a natural substance in a solvent such as water or an organic solvent, an extract obtained by extracting a specific component of a natural substance, and the like. In a specific embodiment of the present invention, the organic solvent is not particularly limited, and may be a C ₁ -C ₅ alcohol having a small carbon number such as methanol, ethanol, isopropanol, n-propanol, n-butanol, isobutanol, or the like. , polyalcohol (polyolcohol) such as glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, etc., hydrocarbon solvents ( Hydrocarbon-based solvent such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, chloroform ( Chloroform), etc., nonpolar organic solvent such as petroleum ether, methyl acetate, benzene, hexane, chloroform, methylene chloride Chloride), dimethyl ether, ethyl acetate and the like.

In a particular embodiment, the extract may be an extract extracted with water, in particular hot water. In another embodiment, the extract may be an extract extracted with a C ₁ -C ₅ alcohol. In still another embodiment, the extract may be an extract extracted with ethanol, but is not limited thereto.

In one form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the extract is an extract extracted with an aqueous solution of water, a C ₁ -C ₅ alcohol or a C ₁ -C ₅ alcohol .

A type, the present invention provides a method to promote hair growth or prevent hair loss composition, wherein the aqueous solution of the C ₁ -C ₅ alcohol is 40-90% (vol / vol). In a specific embodiment, the concentration of the aqueous solution of the C ₁ -C ₅ alcohol may be 40% or more, 45% or more, 50% or more, 55% or more, 60% or more, 65% or more, 66%. Or above, 67% or more, 68% or more, 69% or more, 70% or more, 71% or more, 72% or more, 73% or more, 74% or more, 75% or more, or 80 %the above. The concentration of the aqueous solution of the C ₁ -C ₅ alcohol may be 90% or less, 85% or less, 80% or less, 75% or less, 74% or less, 73% or less, 72% or less, 71% or Hereinafter, 70% or less, 69% or less, 68% or less, 67% or less, 65% or less, or 60% or less. When the aqueous solution concentration of the C ₁ -C ₅ alcohol is within the above range, an excellent effect of preventing hair loss or promoting hair growth is achieved.

In one form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the C ₁ -C ₅ alcohol is one or more selected from the group consisting of methanol, ethanol, isopropanol, n-propanol, and A group consisting of butanol and isobutanol.

In one form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the aqueous solution of the C ₁ -C ₅ alcohol is an aqueous solution of ethanol having a concentration of 60-80%.

In one version of the invention, the ginseng flower shaft extract may comprise from 0.1 to 90% by weight based on the total weight of the composition. In a particular embodiment, the ginseng flower shaft extract can comprise from 10 to 85% by weight based on the total weight of the composition. In another embodiment, the ginseng flower shaft extract may comprise from 20 to 80% by weight based on the total weight of the composition.

In one form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the ginseng flower axis is a ginseng flower axis from which ginseng berries have been removed.

In one form, the invention provides a composition that promotes hair growth or prevents hair loss, wherein the composition promotes proliferation of dermal papilla cells.

In one form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the composition for promoting hair growth or preventing hair loss is a food, pharmaceutical or cosmetic composition.

In one form, the present invention provides a composition for promoting hair growth or preventing hair loss, wherein the composition is formulated into a powder, a granule, a tablet, a capsule, a suspension, an emulsion, a syrup, an aerosol, and a topical application. Formulation, suppository, and sterile injectable solution.

In one form of the invention, the pharmaceutical composition further comprises a suitable carrier, excipient or diluent which is commonly used in the preparation of pharmaceutical compositions. In one form, the carrier, excipient or diluent which may be included in the composition may include lactose, dextrose, sucrose, sorbitol, mannitol, Xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate ), calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate (methyl hydroxybenzoate), propyl hydroxybenzoate, talc, magnesium stearate, mineral oil, and the like.

The pharmaceutical composition can be formulated into a formulation for oral administration such as powder, granule, tablet, capsule, suspension, emulsion, syrup, aerosol, etc., external preparation, suppository according to a usual method. Or sterile injection solution.

Usually, the formulation uses a diluent, an excipient or the like such as a filler, an extender, a binder, a wetting agent, a disintegrant. , surfactant, etc. are prepared. Solid preparations for oral administration may include tablets, pills, powders, granules, capsules and the like. In addition to the active ingredient, the solid preparation may contain at least one excipient such as starch, calcium carbonate, sucrose, lactose or gelatin. In addition to simple excipients, a lubricant such as magnesium stearate or talc may be further included. Liquid preparations for oral administration may include suspensions, internal liquid preparations, emulsions, syrups and the like. In addition to simple diluents such as water or liquid paraffin, various excipients such as wetting agents, sweeteners, aromatics, preservatives and the like may also be included. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations or suppositories. As the nonaqueous solution or suspension, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As a base for suppositories, witepsol, macrogol, tween 61, laurin butter, cocoa butter, cocoa butter, can be used. , glycerol gelatin (glycerogelatin) and the like.

The dose of the extract to be administered in the present invention varies depending on the physical condition and body weight of the patient, the severity of the disease, the type of the drug, the route of administration, and the time of administration, and can be selected within a generally accepted range. In one form, the active ingredient can be administered in a daily dose of 0.0001 to 1000 g/kg, specifically 0.001-1 g/kg, based on the dry weight of the active ingredient.

In one form of the invention, the composition can be a cosmetic composition. The cosmetic composition of the present invention can be prepared into any preparation commonly used in the art. Examples include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants. Surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but are not limited thereto.

In one form of the invention, the composition may be a food composition in the form of various foods, beverages, chewing gums, teas, vitamin complexes, health food supplements, and the like, and may be powders, granules, tablets. Agent, capsule or liquid. Each of the preparations of the food composition can be prepared by a person skilled in the art according to the formulation or the purpose of use, without difficulty in mixing the active ingredient with ingredients commonly used in the art. A synergistic effect can be achieved when the active ingredient is used with other ingredients.

Generally, the amount of active ingredient contained in the food or beverage may range from 1 to 5% by weight in the health food composition and from 0.02 to 10 grams, specifically from 0.3 to 1 gram per 100 milliliters of the health beverage composition.

In one form, the composition may contain other ingredients that provide a synergistic effect on the main effect, without adversely affecting the primary effect of the invention. For example, it may also contain fragrances, pigments, sterilizers, antioxidants, antiseptics, humectants, thickeners, minerals, emulsifiers. (Emulsifier), synthetic polymer or the like as an additive for improving physical properties. In addition, it may also contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polypeptides, polysaccharides, and seaweed extracts. These ingredients can be appropriately selected by those skilled in the art without difficulty according to the formulation or the purpose of use, and the amount of addition can be appropriately selected within the range not adversely affecting the object and effect of the present invention. For example, the ingredients may be added to the amount of from 0.01 to 5% by weight, more specifically from 0.01 to 3% by weight, based on the total weight of the composition.

The composition according to the present invention may be in various preparation forms including solutions, emulsions, viscous mixtures, tablets, powders and the like, and may be administered by various methods including simple drinking, injection, spraying, extrusion, etc. .

Hereinafter, the present invention will be described in detail through examples. However, the following examples are for illustrative purposes only, and the scope of the invention is not limited by the examples. Example 1 and 2 : Preparation of ginseng flower shaft extract

In order to prepare the ginseng flower shaft extract, the harvested ginseng flower shaft is dried or hot air dried after removing the fruit portion. One kilogram of dried ginseng flower shaft was added to 10 liters of 70% ethanol (Example 1) or water (Example 2), refluxed, filtered, and concentrated under reduced pressure at 40-45 °C.

The obtained ginseng flower shaft extract was used in the following test examples. Test Example 1 : Evaluation of the effect of promoting proliferation of dermal papilla cells

To investigate whether the ginseng flower shaft extracts of Examples 1 and 2 were effective in preventing hair loss and promoting cell-level hair growth, their effects on proliferation of dermal papilla cells were tested.

This experiment used rat sputum dermal papilla cells donated by Dr. W. Felsell (University of Cambridge, UK) (PVGDPLTt sa 6 cells are polyomaviruses encoded by temperature-sensitive T antigens transfecting) The T gene (polyomavirus large T gene) is obtained from the dermal dermal papilla cells of PVG rats, hereinafter referred to as DP cells. The effects of the ginseng flower shaft extracts of Examples 1 and 2 on the proliferation of dermal papilla cells were evaluated.

First, DP cells were prepared on a 96-well plate at a concentration of approximately 10 ⁴ cells/well. The ginseng flower shaft extracts of Examples 1 and 2 were dissolved in dimethyl hydrazine or ethanol and serially diluted with Dulbecco's Modified Eagle's Medium (DMEM) containing 5% fetal bovine serum (FBS). To a final concentration of 0.1, 1 or 10 ppm. 200 μl of the medium containing the ginseng flower shaft extract or the control of Examples 1 and 2 was added to each well, and the cells were incubated in a 5% carbon dioxide (CO ² ) incubator at 37 ° C for about 3 days.

After 50 microliters of 2 mg/ml cell viability test reagent (MTT) solution was added to each well, the cells were incubated for 4 hours in the same incubator. After the cell viability test reagent was absorbed into the mitochondria of the cells, the supernatant was removed and 150 microliters of dimethyl hydrazine was added. Then, the formazan dye was dissolved by stirring for 10 minutes. Subsequently, an optical density value (O.D. value) of 515 nm was measured using an ELISA reader, and the number of cells was calculated using a standard curve. The ability to promote proliferation of dermal papilla cells (%) was calculated from the number of cells.

The ability to promote proliferation of dermal papilla cells is shown in Figure 1.

As shown in Fig. 1, it can be seen that the ginseng flower shaft extracts of Example 1 (70% ethanol extract) and Example 2 (water extract) exhibit different excellent effects for promoting the proliferation of dermal papilla cells depending on the dose. . Therefore, it can be seen that the ginseng flower shaft extract exhibits an excellent effect in preventing hair loss, promoting hair growth, and enhancing hair health by promoting proliferation of dermal papilla cells. Test Example 2: through hair follicles (hair follicles) organ culture (organ culture) Evaluation of Effect on hair growth

The effect of the ginseng flower shaft extract (70% ethanol extract) in Example 1 on hair growth was evaluated by organ culture of hair follicles to investigate the effects of organ-level prevention of hair loss and hair growth promotion.

The experiment was carried out as follows. - Separation of human hair follicles: The occipital biopsy tissue obtained from the dermatology department of Dankook University Hospital was placed in cold phosphate buffered saline (PBS). After trimming to the size of the follicular units, the biopsy tissue is cut into the area below the sebaceous gland, as shown in Figure 2. - Information about human hair follicle samples (No. 1: 52-year-old male, No. 2: 57-year-old male). - Use 10-15 follicular units per test group. - Hair follicle culture: After transferring 500 μl of hair follicles to a 24-well disc, 6 samples were cultured per well. - culture medium: William's E medium with L-glutamine (2 mM), hydrocortisone (10 ng/ml), antibiotics (antibiotics) 1%), insulin (10 μg / ml) and fungus intravenous injection (fungizone) supplement, every 3 days. - Treatment: The ginseng flower shaft extract of Example 1 was dissolved in dimethyl sulfoxide (DMSO) or ethanol and serially diluted to a final concentration of 10 or 20 μg/ml using the same medium. - Measurement of hair growth: Hair follicles were imaged using a dissection microscope, and hair growth on day 2, day 5 and day 7 was measured using image analysis software ImageJ. The results of imaging the No. 2 hair follicle sample on day 5 are shown in Figure 2. The results of measuring hair growth of No. 2 hair follicle samples on Days 2 and 5 are shown in Figure 3.

As can be seen from Fig. 2 and Fig. 3, the ginseng flower shaft extract of Example 1 was effective in promoting hair growth of hair follicle tissues derived from humans when treated at a concentration of 20 ppm. Therefore, it was confirmed that the ginseng flower shaft extract exhibited excellent effects for preventing hair loss, promoting hair growth, and enhancing hair health.

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Fig. 1 shows the results of a test for promoting the proliferation of dermal papilla cells by the ginseng flower shaft extract obtained in Examples 1 and 2 according to the present invention in Test Example 1.

Fig. 2 and Fig. 3 show the test results of the organ culture of the ginseng flower shaft extract obtained in Example 1 of the present invention through the hair follicle in Test Example 2.

Claims (9)

A composition for promoting hair growth or preventing hair loss, comprising a ginseng flower shaft extract as an active ingredient. The composition for promoting hair growth or preventing hair loss according to claim 1, wherein the extract is an extract extracted with an aqueous solution of water, a C ₁ -C ₅ alcohol or a C ₁ -C ₅ alcohol. The composition for promoting hair growth or preventing hair loss according to claim 2, wherein the aqueous solution of the C ₁ -C ₅ alcohol has a concentration of 40 to 90% (vol/vol). The composition for promoting hair growth or preventing hair loss according to claim 2, wherein the one or more C ₁ -C ₅ alcohols are selected from the group consisting of methanol, ethanol, isopropanol, n-propanol, n-butanol and iso A group consisting of butanol. The composition for promoting hair growth or preventing hair loss according to claim 2, wherein the extract is an extract extracted using an aqueous ethanol solution having a concentration of 60-80% (vol/vol). A composition for promoting hair growth or preventing hair loss according to claim 1, wherein the ginseng flower axis is a ginseng flower shaft from which ginseng berries have been removed. The composition for promoting hair growth or preventing hair loss according to any one of claims 1 to 6, wherein the composition promotes proliferation of dermal papilla cells. The composition for promoting hair growth or preventing hair loss according to any one of claims 1 to 6, wherein the composition for promoting hair growth or preventing hair loss is a food, pharmaceutical or cosmetic composition. The composition for promoting hair growth or preventing hair loss according to claim 8, wherein the composition is formulated into a powder, a granule, a tablet, a capsule, a suspension, an emulsion, a syrup, an aerosol, a topical formulation, a suppository, and a sterilizing agent. One of injection solutions and the like.