KR20090129928A - Composition for applying to skin externally containing extract from flower of ginseng - Google Patents

Abstract

PURPOSE: A skin external composition containing ginseng flower extract is provided to prevent and treat dry skin and ensure anti-aging, anti-wrinkle, skin elasticity, whitening, and moisturization. CONSTITUTION: A skin external composition contains ginseng flower extract as an active ingredient. The ginseng flower extract is contained in 0.001-10 weight% based on total weight. A method for producing ginseng flower extract comprises: a step of adding organic solvent(ethanol, methanol, butanol, ether, ethylacetate, chloroform, etc) containing water to dried ginseng flower; a step of placing mixture at room temperature for one day; a step of removing chlorophyll; and a step of concentrating in vacuum condenser.

Description

Composition for applying to skin externally containing extract from flower of ginseng}

The present invention relates to the use of ginseng flower extract. More specifically, the present invention is a skin protection use, anti-aging use, anti-wrinkle use, skin elasticity improvement use, whitening use, skin moisturizing use, prevention and improvement of skin dry diseases such as atopy, sebum control use of ginseng flower extract , Anti-inflammatory use, prevention or improvement of skin troubles such as acne, exfoliation, improvement of skin color, prevention of hair loss, anti dandruff, improvement of gloss and softness of hair, enhancement of volume and elasticity of face or body, improvement of lines of face or body It is about a use.

Panax ginseng C.A.Meyer is a plant belonging to the genus Ogapiaceae ginseng, and has been used in Korea, China and Japan for more than 2,000 years. It has been used to prevent disease and extend life. Ginseng's known effects and effects to the central nervous system, anti-carcinogenic and anti-cancer activity, immune function regulation, anti-diabetic effect, liver function anti-inflammatory effect, cardiovascular disorder improvement and anti-arteriosclerosis, blood pressure control action, menopausal Effects on improving disorders and osteoporosis, antistress and anti-fatigue, antioxidant activity and anti-aging effects are known.

Ginsenoside, a representative physiologically active component of ginseng, is evenly distributed on the ground and underground parts of ginseng. However, ginsenoside content and composition differ greatly depending on the parts of ginseng root (root), ginseng leaf, ginseng fruit. In particular, ginseng flowers have been reported to have high hepatoprotective efficacy unlike other parts based on ginseng root (root) and other ginsenoside components and ingredients (J. Nat. Prod, 66; 922-927, 2003.) .

Recently, as consumers' interest in natural cosmetics increases and many cosmetics using herbal ingredients are released, ginseng is also being researched as a plant material having important skin effects of cosmetics. However, most of them use ginseng root. Specifically, only ginsenoside components and ginseng polysaccharides of the ginseng root extract or ginseng root, using the ginseng polysaccharide, there is no study on the skin efficacy by the components of the ginseng flower.

An object of the present invention is to provide a skin external preparation useful for skin.

An object of one embodiment of the present invention, to provide a composition for protecting the skin.

It is an object of another embodiment of the present invention to provide a composition for protecting hair.

It is an object of another embodiment of the present invention to provide a composition for preventing or improving aging.

It is an object of another embodiment of the present invention to provide a composition for preventing or improving wrinkles.

Another object of an embodiment of the present invention, to provide a composition for improving skin elasticity.

Another object of one embodiment of the present invention, to provide a composition for skin whitening.

Another object of one embodiment of the present invention, to provide a composition for moisturizing the skin.

Another object of one embodiment of the present invention, to provide a composition for preventing or improving skin dry diseases such as atopy.

 It is another object of an embodiment of the present invention to provide a composition for removing dead skin cells.

Another object of an embodiment of the present invention is to provide a composition for controlling sebum of the skin.

It is an object of another embodiment of the present invention to provide a composition for preventing or alleviating inflammation.

It is an object of another embodiment of the present invention to provide a composition for preventing or alleviating inflammation of the scalp.

Another object of one embodiment of the present invention, to provide a composition for preventing or improving skin problems such as acne.

Another object of an embodiment of the present invention is to provide a composition for preventing or improving skin troubles such as acne occurring on the scalp.

It is an object of another embodiment of the present invention to provide a composition for improving the color of the skin.

Another object of an embodiment of the present invention is to provide a composition for preventing hair loss.

Another object of one embodiment of the present invention, to provide a composition for anti-dandruff.

It is an object of another embodiment of the present invention to provide a composition that promotes the gloss or softness of hair.

It is an object of another embodiment of the present invention to provide a composition for enhancing the volume and elasticity of the face or body.

It is an object of another embodiment of the present invention to provide a composition for improving the line of the face or body.

In order to achieve the above object, the external preparation composition for skin according to the present invention is characterized by containing ginseng flower extract as an active ingredient.

In one embodiment of the present invention, the external preparation composition for skin is characterized in that it has a use of protecting skin by inhibiting skin damage. The skin here includes the scalp or hair. That is, in the present specification, a topical skin composition for protecting hair by inhibiting damage to the scalp or hair is disclosed.

In another embodiment of the present invention, the external preparation composition for skin is characterized by protecting the skin through the action of inhibiting skin damage caused by oxidation or ultraviolet rays. The skin here includes the scalp or hair. That is, a topical skin composition for protecting the scalp or hair by inhibiting damage to the scalp or hair by oxidation or ultraviolet rays is disclosed.

In another embodiment of the present invention, the external preparation composition for skin is characterized by having a use for inhibiting skin aging.

In another embodiment of the present invention, the external preparation composition for skin may be obtained by inhibiting skin damage caused by oxidation or ultraviolet rays, promoting collagen production or inhibiting biosynthesis of matrix metalloproteinase I (MMP-1). It is characterized by suppressing aging.

In another embodiment of the present invention, the external preparation composition for skin is characterized in that it has a use for preventing or improving skin wrinkles.

In another embodiment of the present invention, the external preparation composition for skin is characterized by preventing or improving skin wrinkles by promoting collagen production or inhibiting biosynthesis of matrix metalloproteinase I (MMP-1).

In another embodiment of the present invention, the external preparation composition for skin is characterized in that it has a use to improve skin elasticity.

In another embodiment of the present invention, the external preparation composition for skin is characterized by having a skin whitening use.

In another embodiment of the present invention, the external preparation composition for skin is characterized in that skin whitening is performed through inhibiting melanin production or whitening of pigments deposited on the skin.

In another embodiment of the present invention, the external preparation composition for skin is characterized by having a skin moisturizing use.

In another embodiment of the present invention, the external preparation composition for skin is characterized by maintaining skin moisture through promoting differentiation of keratinocytes or promoting expression of transglutaminase.

In another embodiment of the present invention, the external preparation composition for skin is characterized by having a use for preventing or ameliorating a skin dry disease including atopic dermatitis or psoriasis.

In another embodiment of the present invention, the external preparation composition for skin is characterized in that it has an application for exfoliation.

In another embodiment of the present invention, the external preparation composition for skin is characterized by having a sebum control use of the skin.

In another embodiment of the present invention, the external preparation composition for skin is characterized by controlling sebum of the skin by inhibiting secretion of sebum.

In another embodiment of the present invention, the external preparation composition for skin is characterized in that it has the use of anti-inflammatory or irritation relief. Here the skin contains the scalp. That is, the present invention provides an external composition for skin having a use for preventing or ameliorating inflammation of the scalp and alleviating irritation to the scalp.

In another embodiment of the present invention, the external preparation composition for skin is characterized in that it has a use for preventing or improving skin troubles including acne. Here the skin contains the scalp. That is, the present specification provides an external composition for skin having a use for preventing skin troubles including acne from occurring on the scalp and improving skin troubles.

In another embodiment of the present invention, the external preparation composition for skin is characterized by having a use for improving skin color.

In another embodiment of the present invention, the external preparation composition for skin is characterized by having the purpose of preventing hair loss.

In another embodiment of the present invention, the external preparation composition for skin is characterized by having an antidandruff use.

In another embodiment of the present invention, the external preparation composition for skin is characterized in that it has a use to enhance the gloss or softness of hair.

In another embodiment of the present invention, the external preparation composition for skin is characterized in that it has a use to enhance the volume and elasticity of the face or body.

In another embodiment of the present invention, the external preparation composition for skin is characterized in that it has a use to improve the lines of the face or body.

In the present invention, by containing the ginseng flower extract having a different ingredient and composition from the ground portion of ginseng as an active ingredient, it protects the skin dry diseases such as skin protection, anti-aging, anti-wrinkle, skin elasticity improvement, whitening, skin moisturizing, atopy Prevention and improvement, sebum control, anti-inflammatory, prevent or improve skin troubles such as acne, exfoliation, improve skin color, prevent hair loss, anti dandruff, improve hair gloss and softness, increase volume and elasticity of face or body, face or There is an effect that can provide a composition for line improvement of the body.

As used herein, the term "extract" includes any material obtained by extracting a component therefrom from a natural product, regardless of the method of extraction or the kind of the component. For example, it is a broad concept that includes all components obtained by extracting a component dissolved in a solvent from a natural product using water or an organic solvent, and those obtained by extracting only a specific component such as an oil such as oil.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is a broad concept including not only tissues covering the body surface such as the face or body, but also the scalp and hair.

In an embodiment of the present invention, the ginseng is Panax ginseng C.A. Meyer.

In an embodiment of the present invention, the ginseng flower extract may be mixed in an amount of 0.001 to 10% by weight based on the total weight of the composition, but is not limited thereto.

Ginseng flower extract according to an embodiment of the present invention, dried organic ginseng flower, including water, water and an organic solvent (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.) or organic solvent 1 to 10 times the ginseng flower It can be prepared by adding (wt / v%) and standing at room temperature for one day. Preferably, the above process is repeated twice to obtain an extract. Further, preferably, after removing chlorophyll from the extract, the filtrate can be concentrated in a vacuum concentrator to obtain a dried product of ginseng flower extract.

Human skin is changed by a number of internal and external factors as it ages. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays reaching the surface of the sun's rays and further increase environmental pollution, free radicals and free radicals, such as free radicals, skin thickness, wrinkles, elasticity is reduced only In addition, the color of the skin becomes dull, skin problems frequently occur, and blemishes, freckles, and mushrooms are also increasing.

As aging progresses, symptoms such as changes and decreases in the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins constituting the skin appear, and are subject to oxidative stress caused by free radicals and active harmful oxygen. In addition, cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokine, which is known to cause inflammation in most cells constituting the skin, due to aging or ultraviolet rays. Biosynthesis increases, and the biosynthesis of matrix metalloproteinase (MMP), an enzyme that breaks down skin tissues by these inflammatory factors, increases NO (nitric oxide) production by inducible nitric oxide synthase (iNOS). It is known to increase. In other words, the biosynthesis of the substrate material is reduced by the reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as the increase of stress caused by various harmful environments and the increase of free radical species caused by sunlight. As a result, decomposition and degeneration are accelerated, and the skin matrix is destroyed and thinned, resulting in various symptoms of skin aging.

The external application composition for skin disclosed herein contains a ginseng flower extract, which is effective in inhibiting the production of free radicals and inhibiting oxidative damage caused by free radicals, as an active ingredient to prevent damage to cells caused by free radicals or ultraviolet rays. You can prevent it and protect your skin. In addition, the progress of aging due to active oxygen or ultraviolet rays can be suppressed. In addition, ginseng flower extract can prevent or improve inflammation by inhibiting the biosynthesis of COX-2, thereby alleviating skin problems. Wherein the skin comprises the scalp. That is, the ginseng flower extract disclosed herein prevents damage to cells caused by free radicals or ultraviolet rays generated from the scalp or hair, prevents or improves inflammation of the scalp, and alleviates stimulation of the scalp by perming or dyeing. Can be used for purposes. In addition, the ginseng flower extract disclosed herein may be used for the purpose of preventing or improving wrinkles of the skin.

Many factors are involved in determining the skin color of humans, including the activity of melanocytes (melanocytes) that make melanin pigment, the distribution of blood vessels, the thickness of skin and the presence or absence of pigments in the body such as carotenoids and bilirubin. It is important.

Especially, the most important factor is a black pigment called melanin which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of this melanin pigment is influenced by genetic factors, physiological factors related to hormone secretion, stress, and environmental factors such as UV irradiation.

Melanin pigment, which is produced from melanocytes of the skin of the body, is a phenolic polymer material having a complex form of black pigment and protein. It protects skin organs below the dermis by blocking ultraviolet rays from the sun and free radicals generated in the skin body. It plays a useful role in protecting proteins and genes in the skin.

As described above, melanin produced by stress stimulation inside and outside the skin is a stable substance that does not disappear until it is discharged to the outside through skin keratinization even if the stress disappears. However, if more melanin is produced than necessary, it causes hyperpigmentation such as blemishes, freckles, and spots, resulting in cosmetically bad results.

The ginseng flower extract disclosed herein can whiten skin by inhibiting the production of melanin. Therefore, the external preparation composition for skin disclosed herein containing ginseng flower extract as an active ingredient can be used for skin whitening.

The most important function of the outermost epidermis of the skin is to protect the skin and protect against various stimuli (physical and chemical stimulating factors such as chemicals, air pollutants, dry environment and ultraviolet rays) from the outside. It is a protective function that prevents excessive divergence of moisture through the body, and this protective function can be maintained only when the stratum corneum composed of keratinocytes is normally formed. The outermost stratum corneum (horney layer) is formed from keratinocytes, and is composed of the differentiated keratinocytes and the surrounding lipid layer. Keratinocytes are characteristic cells whose basal cells, which continuously proliferate in the epidermal layer, undergo morphological and functional changes in stages and rise to the surface of the skin. Exfoliated and new keratinocytes take over their functions. This repetitive sequence of changes is called "epidermal differentiation" or "keratinization." During keratinization, keratinocytes form the stratum corneum by producing natural moisturizing factor (NMF) and intercellular fat (ceramide, cholesterol, fatty acids), making the stratum corneum firm and flexible. It has a function as).

However, these stratum corneum can easily lose its function due to excessive face washing, lifestyle factors such as bathing, environmental factors such as dry air and pollutants, and endogenous diseases such as atopic or aged skin. In fact, due to various factors that have increased in modern times, more and more people are complaining of dry skin symptoms and all kinds of obstacles. Therefore, many studies have been conducted to supply moisture from the outside or to prevent water loss from the body in order to maintain proper skin moisture, and in fact, various types of moisturizers having moisture retention capabilities have been developed and mainly cosmetics. It is widely used in the field.

However, as the risk factors for humans increase and the aged population rapidly increases in the living environment, the turnover rate of the stratum corneum is slowed, the lipid synthesis ability of the keratinocytes is degraded, or the epidermis is normal in the epidermis. Due to poor cell division, maturation and differentiation, the amount of moisturizing factors and lipids in the stratum corneum is reduced, so that humans with skin with a condition that is unable to maintain normal stratum corneum function, that is, the skin barrier function is deteriorated There is a growing trend.

Due to the abnormal division and differentiation of epidermal cells, the skin causes various skin diseases such as dry skin, atopy and psoriasis, and these diseases can alleviate the symptoms slightly by using a conventional moisturizer having only water retention function. However, it is difficult to expect radical healing.

The ginseng flower extract disclosed in the present specification promotes differentiation of keratinocytes, thereby assisting in the process of `` epidermal cell differentiation '' or `` keratinization '', a process of eliminating old keratinocytes and producing new keratinocytes, and thus keratinocytes. It has the effect of effectively removing the. The external preparation composition for skin disclosed herein may be usefully used as an exfoliation composition by containing ginseng flower extract having an excellent exfoliation effect as an active ingredient.

In addition, the ginseng flower extract disclosed in the present specification, by maintaining the turn-over rate of the stratum corneum in the normal range to enable the normal amount of moisturizing factors and lipids in the stratum corneum. It also promotes trans glutamine expression. Through this, it is possible to moisturize the skin.

In addition, the ginseng flower extract disclosed herein can enhance the skin barrier function by promoting the differentiation of keratinocytes and maintaining skin moisture, thereby preventing or treating skin dry diseases such as atopy or psoriasis.

In general, the sebum in the skin including the scalp and face plays a role in maintaining the moisturizing of the skin and preventing the invasion of microorganisms, but when the sebum is excessively secreted, the sebum is excessively promoted, hair loss becomes worse, acne enlarged pores Is caused and several diseases are caused, including seborrheic dermatitis.

The secretion of sebum is caused by a number of causes, the most important of which is the activation of sebaceous gland cells by the amount of dihydrotestosterone (DHT), one of the hormones involved in promoting sebaceous gland secretion. It is oversecreted. That is, in hair loss, testosterone (T) is converted into dihydrotestosterone which is one of the male hormones by 5-alpha-reductase type 2 in the cell and at the same time the receptor in the cytoplasm. It binds to the protein and enters the fluid, causing hair loss, but in the skin or sebaceous glands, testosterone is converted to dihydrotestosterone by 5-alpha-reductase type 1, which activates sebaceous gland cells. Differentiation is promoted by oversecreting sebum in the sebaceous glands, causing acne.

Acne and hair loss, skin problems that occur in addition to the excessive secretion of sebum is further exacerbated by the skin's fine inflammatory response. The cause of acne is that excessive sebum accumulates in the hair follicles, thereby accommodating the activity of acne bacteria and causing inflammation.

Ginseng flower extract disclosed herein can prevent or improve acne, pores enlargement, seborrheic dermatitis, etc. due to sebum secretion by inhibiting sebum secretion.

The external application composition for skin disclosed herein may enhance the volume and elasticity of the face and body by promoting the differentiation of fat cells. When the external application composition for skin disclosed in the present specification is applied to the face and the body in which volume and elasticity are required to be enhanced, volume and elasticity are enhanced. Sites in which volume and elasticity enhancement are desired may be sites recessed due to aging. For example, the cheek meat etc. depressed by aging are mentioned. Applying the topical skin composition disclosed herein to the depressed cheeks, the volume can be enhanced by promoting adipocyte differentiation. In addition, other examples of negatives that require increased volume and elasticity include breast and hip areas. Applying the skin topical composition disclosed herein to the chest and buttocks has the effect of making the chest and buttocks elastic and voluminous.

In addition, the topical skin composition disclosed herein may improve the lines of the face and body. Ginseng flower extract as an active ingredient promotes the differentiation of fat cells to promote the volume and elasticity of the face and body, promote the biosynthesis of collagen and inhibit the breakdown of collagen, so that the face and body damaged due to aging, etc. To improve the line.

The cosmetic form according to the invention contains a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing the oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases, and It may be provided in the form of a nonionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

Cosmetics of the present invention may be used in the preparation of fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers. Cosmetics such as fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics or It may contain adjuvants commonly used in the field of dermatology. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts.

The external preparation for skin containing the ginseng flower extract of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing It may be formulated into a cosmetic such as cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil and body essence.

The external preparation composition for skin according to the present invention may be a pharmaceutical composition. The pharmaceutical composition may further contain pharmaceutical adjuvants such as preservatives, stabilizers, hydrating or emulsifying accelerators, salts for regulating osmotic pressure and / or buffers and other therapeutically useful substances. The pharmaceutical composition may be formulated as a lotion, cream, ointment or gel.

The pharmaceutical composition is preferably administered transdermally.

Dosage of ginseng flower extract as an active ingredient of the pharmaceutical composition will vary depending on the age, sex and weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber. . Dosage determination based on these factors is within the level of skill in the art. Generally dosages range from 0.0001 mg / kg / day to approximately 2000 g / kg / day.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to Examples and Test Examples. However, these experimental examples and examples are provided only for the purpose of illustration in order to help the understanding of the present invention is not limited to the scope and scope of the present invention.

Example 1 Preparation of Ginseng Flower Extract

Into the dried ginseng flower 1kg, 70% (v / v) ethanol 10L was added and extracted at room temperature for one day. The above process was repeated twice to obtain an extract, and the obtained extract was filtered and the filtrate was concentrated to 2 L in a vacuum concentrator. Chlorophyll was then removed by adding the same amount of ether to remove the ether layer from the filtrate. The above extraction was repeated three times, and the remaining extract was concentrated in a vacuum concentrator to obtain 150 g of dried matter.

Example 2, Comparative Example 1

The composition containing the ginseng flower extract prepared in Example 1 was prepared by mixing according to the composition of Table 1.

Ingredient (% by weight) Example 2 Comparative Example 1 Ginseng Flower Extract (Example 1) 0.01 - Caprylic Capric Triglyceride 70 80 ethanol 10 10 water Remaining amount Remaining amount

[Test Example 1] free radical production inhibition experiment

Antioxidant effect was investigated through experiments comparing the ability to remove reactive oxygen species (ROS) generated from cells by ultraviolet (UV) irradiation. As a positive control group, vitamin C (Sigma. Co.), which is generally used to compare antioxidant effects, was used. In addition, the group not treated with the test substance was used as a control. An extract prepared according to Example 1 was treated with ultraviolet (UVB 30 mJ / cm ² ) in a human HaCaT keratinocytes monolayer culture system (P Boukamp, German Cancer Research Center, Heidelberg.German). 10 ppm, 50 ppm and 100 ppm were respectively treated. Then, DCFH-DA (2 ', 7'-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc) was measured by a fluorescent plate reader. The control group not treated with UV was set to 100 and the ratio of ROS generation was calculated as a ratio thereof. The results are shown in Table 2 and FIG.

Test substance ROS generation rate (%) Non-UVB 100 UVB 161 UVB + Example 1 (10 ppm) 78 UVB + Example 1 (50 ppm) 72 UVB + Example 1 (100 ppm) 70 UVB + Vitamin C 100 uM 72

The ginseng flower extract of Example 1 according to the present invention showed an effect of significantly scavenging the active oxygen generated by UV compared to the control. This is an excellent activity similar to that of vitamin C used as an indicator of antioxidant activity. That is, the ginseng flower extract according to Example 1 of the present invention protects the skin including the scalp and hair by significantly eliminating active oxygen which causes skin damage, and furthermore, to prevent wrinkles, elasticity and pigmentation, etc. It was confirmed that it can have a prophylactic effect.

Experimental Example 2 Inhibitory Activity Against DNA Oxidative Damage by Single Cell Gel Electrophoresis (SCGE, Comet Assay)

After culturing human fibroblasts for 24 hours, the ginseng flower extract and vitamin E of Example 1 were treated by concentration, and after 12 hours, the positive control group H ₂ O ₂ (final concentration 10-3M) was treated. The result of staining by the electrophoresis method was observed by fluorescence microscope. At this time, the length (um) of tails resulting from DNA cleavage in each of 25 cells was analyzed using KOMET 3.1 (Kinetic Imaging, England), an image analyzer. 2 is shown.

Test substance Tail length (μm) % Inhibition H ₂ O ₂ 112.4 - H ₂ O ₂ + Example 1 (10 ppm) 104.6 6.9 H ₂ O ₂ + Example 1 (50 ppm) 70.2 37.5 H ₂ O ₂ + Example 1 (100 ppm) 65.4 41.8 H ₂ O ₂ + Vitamin E 10 ppm 68.9 38.7

In the results of Table 3, the ginseng flower extract of Example 1 by cell gel electrophoresis showed significant oxidative DNA damage inhibitory activity, whereby the ginseng flower extract according to the present invention is a hydroxyl radical (.OH) It was confirmed that it acts as a DNA oxidative damage inhibitor that inhibits the DNA single strand break. This is an important protective effect of protecting the genes of cells of the skin, including the scalp or hair.

Experimental Example 3 Type I Procollagen Assay

Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ and then replaced with a medium containing 1 ppm, 10 ppm, 50 ppm of ginseng flower extract of Example 1. Cells were harvested on the third day of culture to quantify the amount of type I procollagen produced by ELISA. TGF-β (Human Transforming Growth Factor-β1, Roche Co.) was used as a positive control. The results were calculated as a comparison with the values measured with the control group not containing the ginseng flower extract of Example 1 as 0 and the positive control group as 100. This is shown in Table 4 and FIG.

Type I Procollagen Biosynthesis (%) Negative control 0 Example 1 (1 ppm) 7 Example 1 (10 ppm) 22 Example 1 (50 ppm) 31 TGF-β 10ng / ml 100

In the normal human fibroblast monolayer culture system, the ginseng flower extract of Example 1 showed an effect of clearly promoting type I procollagen production as compared to the control group. That is, the ginseng flower extract of Example 1 may promote collagen production to alleviate collagen reduction caused by aging of human skin.

Experimental Example 4 Analysis of MMP-1 Expression Inhibition

Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ and then irradiated with ultraviolet B at 40 mJ / cm ² , and then replaced with a medium containing 1 ppm, 10 ppm and 50 ppm of ginseng flower extract of Example 1. Cells were harvested on the second day of culture (harvest) to quantify the amount of matrix metalloproteinase I (MMP-1) produced by ELISA. The result was calculated as a comparison with the value measured using a control group not irradiated with UV light containing no test substance 100, the positive control group was used RA (Retinoic acid , Sigma. Co.).

MMP-1 biosynthesis (%) No-UV 100 UV 152 UV + Example 1 (1 ppm) 128 UV + Example 1 (10 ppm) 105 UV + Example 1 (50 ppm) 92 UV + RA 10 uM 45

The ginseng flower extract of Example 1 according to the present invention in human normal fibroblast monolayer culture system significantly inhibited the expression of MMP-1 induced by UVB 40 mJ / cm ² . Therefore, the ginseng flower extract of Example 1 according to the present invention inhibits the biosynthesis of the skin tissue degrading enzyme MMP-1 caused by internal aging or external environmental factors, which can inhibit the reduction of collagen in the skin and improve wrinkles. will be.

[Experiment 5] Skin wrinkle improvement effect for human skin

For 40 subjects with facial wrinkles aged 30 to 50 years, except the nutrition cream containing the ginseng flower extract of Example 1 having the composition shown in Table 5 and the ginseng flower extract were not contained. And after preparing the nourishing cream of the same composition (Comparative Example 2) as in Example 3, the skin wrinkle improvement effect was compared and evaluated (unit: weight%).

ingredient Example 3 Comparative Example 2 Ginseng flower extract 0.1 - Beeswax 10 10 Polysorbate 60 1.5 1.5 Sebum 60 Cured Castor Oil 2 2 Sorbitan sesquioleate 0.5 0.5 Liquid paraffin 10 10 Squalane 5 5 Caprylic / Capric Triglycerides (Estasan: Uniqema) 5 5 glycerin 5 5 Butylene glycol 3 3 Propylene glycol 3 3 Triethanolamine 0.2 0.2 Preservative, coloring, flavoring Quantity Quantity Purified water Remaining amount Remaining amount

Example 3 was used for the left side of the subject and Comparative Example 2 was used for the right side for 3 months. The skin condition was measured on both sides of the face before the use of the cream, and three months after the use of the cream, the same area was remeasured to measure the change in skin wrinkles. In the constant temperature and humidity room of 24 ° C. and 40% RH, the wrinkles at the tail of the eyes were replicated, and skin wrinkles were measured using a Visiometer system (C + K). The amount of change in skin wrinkles was calculated according to Equation 1 below.

In Equation 1, "Tdi" is a measurement site value at D90, and "Tdo" is a measurement site value at D0.

As a result of the calculation according to Equation 1, the skin wrinkle of the site using Comparative Example 2 showed a decrease of 6.1 ± 7% (mean ± standard deviation), while the skin wrinkle of the site using Example 3 was 35 ± 8%. It showed a reduction of, showing an excellent skin wrinkle improvement effect.

Experimental Example 6 Experiment of Skin Elasticity Improvement Effect in Human Body

The skin elasticity improving effect of Example 3 and Comparative Example 2 prepared by the composition of Table 6 was measured. Forty women over 30 years old were divided into two sets, and each set of nutritional creams of Example 3 and Comparative Example 2 was applied to the face twice a day for 12 weeks, followed by a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic). Co., Germany) to measure skin elasticity. As a result of calculating the results, the elasticity effect of the site using Comparative Example 2 showed 0.21 ± 0.14 (mean ± standard deviation), while the elasticity effect of the site using Example 3 showed 0.30 ± 0.10, thereby improving the skin elasticity. Indicated. The result value refers to the nature of the skin viscoelasticity of the skin elasticity measuring instrument.

As shown in the results, when using the nutrition cream of Example 3 containing the ginseng flower extract of Example 1 showed about 140% skin elasticity enhancing effect.

Experimental Example 7 Inhibitory Effects of Melanin Production Using Pigment Cells

In order to investigate the melanin production inhibitory effect of the ginseng flower extract of Example 1, the mouse pigment cells were used.

First, mouse Mel-Ab cells derived from C57BL / 6 mice (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) were placed in DMEM (Dulbeccos modified Eagles media) in 10% fetal placental serum, 100 nM. The culture was carried out at 37 ° C. and 5% CO ₂ in a medium to which 2-O-tetracanocanophorbol-13-acetate and 1 nM cholera toxin were added. Cultured Mel-Ab cells were detached with 0.25% trypsin-EDTA, incubated at a concentration of 10 ⁵ cells / well in 24-well plates, and then 10 ppm, 50 ppm and 3 consecutive days from day 2 100 ppm ginseng flower extract (Example 1) was added and cultured. At this time, arbutin (arbutin, Sigma Co.) was used as a positive control group. Then, the culture medium was removed, washed with PBS, the cells were dissolved with 1N sodium hydroxide, the absorbance was measured at 400 nm, and then the melanin production inhibition rate was calculated according to Equation 2 below, and the results are shown in Tables 7 and 5 below. (Dooley's method).

Melanin production inhibition rate (%) Control (untreated) 0 Example 1 (10 ppm) 4 Example 1 (50 ppm) 25 Example 1 (100 ppm) 49 Arbutin 100 ppm 52

As shown in Table 7, it was confirmed that the ginseng flower extract of Example 1 according to the present invention showed melanin production inhibition rate similar to that of hydroquinone. Through this, the ginseng flower extract of Example 1 according to the present invention was confirmed to exhibit an excellent whitening effect.

Experimental Example 8 Whitening Effect on Human Skin

In order to determine the whitening effect of the ginseng flower extract of Example 1 on the human skin was carried out the following experiment.

First, a opaque tape with a hole of 1.5 cm in diameter was attached to the upper arm of 12 healthy men, and then 1.5 ~ 2 times of ultraviolet light (UVB) of each subject's minimum erythema (Minimal Erythema Dose). Was irradiated to induce skin blackening.

After the UV irradiation, the ginseng flower extract and hydroquinone of Example 1, which were test substances, were applied to each, and one place was observed for 10 weeks without applying anything. Skin color was measured on a weekly basis with a colorimeter CR2002 (Minolta, Japan).

Then, the difference in skin color (ΔL *) at the start of the application and the completion of the application of each test substance was calculated according to Equation 3 below, and the results are shown in Table 8 below. On the other hand, the whitening effect is determined by comparing ΔL * between the sample application site and the control site. When ΔL * value is about 2, the whitening effect of the deposited pigment is clear, and when it is about 1.5 or more, the whitening effect is determined. Can be.

ΔL * = L * value at the time of completion of coating-L * value at the start of coating

Test substance Skin color brightness degree (△ L *) Example 1 1.91 ± 0.17 Hydroquinone (positive control) 1.89 ± 0.15 Vehicle (negative control) 0.51 ± 0.13

As shown in Table 8, it was confirmed that the ginseng flower extract of Example 1 according to the present invention showed a skin color brightness similar to that of hydroquinone. This is because the ginseng flower extract improves the pigmentation produced by ultraviolet light and brightens the skin color.

Experimental Example 9 Induction of Differentiation of Human Keratinocytes

The differentiation effect of the ginseng flower extract of Example 1 according to the present invention was measured by measuring the amount of CE (Cornified Envelop) produced during the differentiation of keratinocytes and human skin cell lines (HaCaT).

After primary cultured human keratinocytes were put in a culture flask and attached to the bottom, the test substances described in Table 9 below were added to the culture medium by concentration, and 5 days until the cells grew to 70-80% of the floor area. Incubated. The cells were harvested and washed with PBS (phosphate buffered saline) and then 10 mM Tris-HCl (Tris-HCl) containing 2% Sodium Dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT). , pH 7.4) 1 ml was added, and the precipitates sonicated, boiled, and centrifuged were again suspended in 1 ml of PBS to measure absorbance at 340 nm. Separately, a portion of the solution after the sonication was taken to measure the protein content, which was used as a reference for evaluating the degree of cell differentiation. The low calcium (0.03mM) treated group and the high calcium (1.2 mM) treated group were negative / positive controls, respectively.

Test substance Differentiation capacity in keratinocytes (%) Control Low calcium group (Ca ²⁺ 0.03 mM) 100 High Calcium Treated Group (Ca ²⁺ 1.2 mM) 215 Example 1 1 ppm 117 10 ppm 122 100 ppm 125

Through the results of Table 9, the ginseng flower extract was confirmed to promote differentiation in keratinocytes. This suggests that skin moisturization can be enhanced by normalizing the turnover cycle, which is the elimination of old keratinocytes and the production cycle of new keratinocytes. In addition, it was confirmed that ginseng flower extract can promote skin exfoliation.

Experimental Example 10 Expression Effect of Transglutaminase in Human Keratinocytes

Human keratinocytes 5 × 10 ⁴ cells were placed in each well of a 96 well cell culture plate and attached for 24 hours. After treatment with the test substance to the attached skin cell line, two days later, the medium was removed, and stored in a -20 ℃ refrigerator. After freeze-thawing was repeated twice, the cells treated with material were destroyed, and then treated with acetone: ethanol (1: 1, v / v) stored at -20 ° C and left at 4 ° C for 30 minutes. The cells were fixed. Thereafter, the organic solvent was allowed to evaporate at room temperature, and treated with blocking (1% bovine serum albumin), transglutaminase antibody, HRP anti-mouse antibody (secondary), and color development was OPD (o). -phennyldiamine) was added. Expression was measured by absorbance at 490nm, calibration was performed by measuring the background (background) at 630nm. Low calcium (0.03mM) treated group and high calcium (1.2 mM) treated group as a negative / positive control, respectively, the test results performed by adding the test substance to the low calcium concentration is shown in Table 10 below.

Test substance Transglutaminase Expression (%) Control Low calcium group (Ca ²⁺ 0.03 mM) 100 High Calcium Treated Group (Ca ²⁺ 1.2 mM) 138 Example 1 1 ppm 98 10 ppm 119 100 ppm 141

In the results of Table 10, the ginseng flower extract of Example 1 according to the present invention showed an excellent expression compared to the control. Therefore, it was found that the ginseng flower extract of Example 1 according to the present invention improves the expression of transglutaminase.

Experimental Example 11 Increased Skin Moisturizing Power in the Human Body

Fifty adult men and women in their fifties and sixties with skin dryness were divided into two groups, and Example 3 and Comparative Example 2 were applied to the face twice daily for four weeks in each group. Before application start, after 1 week, 2 weeks, 4 weeks after application, and after 2 weeks (total 6 weeks) after application was stopped, constant temperature, constant humidity (24 ° C, 40% relative humidity) was used as a comonometer. Skin moisture was measured and the results are shown in Table 11 below. The test result is a percentage of the increase of the measured value after a certain period of treatment based on the Koniometer value measured immediately before the test was started.

Moisture Growth Rate (%) Test substance 1 week past 2 weeks past 4 weeks past After 6 weeks Example 3 33 41 42 33 Comparative Example 2 31 34 34 15

The Koniometer is a skin moisture meter that measures the amount of moisture present on the skin by measuring the conductivity of the epidermis. As shown in Table 11, the amount of skin moisture was increased in the group coated with the material of Example 3 containing the ginseng flower extract of Example 1 compared to the control group applied to Comparative Example 2. Also, two weeks after the application of the nourishing cream of Example 3 (6 weeks in total), the skin moisture levels were similar to those after 1 to 2 weeks. During the period it was found that the skin moisture by the application of the ginseng flower extract according to the present invention is continuously maintained.

Experimental Example 12 Sebum Secretion Inhibitory Effect

To measure the effects of oily skin, 20 to 45-year-old females with oily skin had 10 cases of Example 3 (with ginseng flower extract) on the forehead, and 10 others of Comparative Example 2 in the morning and evening. After application twice a day to use, after 1 week, 2 weeks, 3 weeks and 4 weeks sebum secretion of the skin was measured using a skin oil meter (Sebumeter SM810). The experiment was carried out at 20 ℃, 20% relative humidity and the measurement results are shown in Table 12. The results are expressed as the average value of 10 subjects. For oily skin the measured value is 220 or more, for normal skin it is between 100 and 220.

Test substance Before application 1 week 2 weeks 3 weeks 4 Weeks Comparative Example 2 246 240 239 235 230 Example 3 248 237 232 216 211

In the results of Table 12, the nutrition cream of Example 3 containing the ginseng flower extract of Example 1 according to the present invention exhibited the effect of inhibiting excessive sebum secretion, it was confirmed that there is a sebum control effect. Therefore, it is possible to prevent acne and troubles caused by excessive sebum occurring on the skin including the scalp.

Experimental Example 13 Inhibitory Effect of Cyclooxygenase-2 (COX-2) Induced by Lipopolysaccharide

Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ and then subjected to LPS treatment, and then the ginseng flower extract of Example 1 was replaced with a medium containing 1 ppm, 10 ppm and 100 ppm. Cells were harvested on the second day of culture (harvest) and the amount of cyclooxygenase-2 (COX-2) produced was quantified using the Western blot method. A comparison with the value measured with a densitometer using the control group as 100 is shown in Table 13 below. At this time, the control group was an untreated group cultured without treating the ginseng flower extract of Example 1.

Test substance (concentration: ppm) COX-2 biosynthesis (%) Example 1 100 56 10 64 One 89 Control 100

As can be seen from the results of Table 13, the ginseng flower extract of Example 1 according to the present invention is to suppress the inflammatory response by reducing the biosynthesis of cyclooxygenase-2 induced by the inflammatory response triggering factor LPS I could see that. In addition, it showed an effect that can alleviate skin problems.

Experimental Example 14 Acne and Skin Trouble Improvement

The number of participants in the experiment was 10 women with severe skin problems including acne. After washing the face with soap in a constant temperature and humidity room for 30 minutes, the skin trouble was measured by comparing the number of acne and the number with inflammation. .

The test subjects were allowed to use the nutrition creams of Example 3 and Comparative Example 2 for 4 weeks, and then the degree of acne and skin troubles was compared, and the results are shown in Table 14 below.

Example 1 (5 persons) Comparative Example 1 (5 persons) Skin trouble improvement rate 86% 32%

As shown in the results of Table 14, the application of Example 3, including the ginseng flower extract of Example 1 according to the present invention for 4 weeks, was shown to reduce the number of existing acne and improve the inflammation accompanying troubles.

Experimental Example 15 Effect of Improving Color on Human Skin (Face)

In order to evaluate the blood circulation promoting effect on the skin of the composition containing the ginseng flower extract of the present invention, the degree of blood circulation in the skin was measured by using a LDPI (Laser Doppler Perfusion Imager). LDPI is a well-known and still used device for measuring blood circulation in the skin. It is a very sensitive device that can measure not only the velocity and amount of blood in the capillaries of the skin but also the flow in the small arteries and the venous veins. .

After washing the face with soap in a thermo-hygrostat room for 30 minutes, the initial values were measured using an IRPI (Infrad) camera, an LDPI and skin temperature measuring instrument. The participants were 20 women with cold hands and feet. LDPI was used to measure the initial blood flow in the lower part of the forehead, and the IR temperature was measured for the forehead, under eye and cheek skin.

After allowing Example 3 to be used by the subjects for one week, blood flow and skin temperature (° C.) were compared with the initial measured values, and the results are shown in Tables 15 and 16, respectively.

Face Before use After 1 week medium 0.945 1.154

forehead Under the eyes cheek Before use (℃) 32.16 32.09 30.34 After 1 week of use (℃) 33.67 33.81 33. 75

As described above, as a result of using Example 3 containing the ginseng flower extract according to the present invention, it was confirmed that the color was improved due to the peripheral blood circulation improvement effect on the faces of the test subjects, which ultimately was the ginseng according to the present invention. Containing a flower extract suggests that it can contribute to the effective delivery of skin nutrients, to inhibit and retard skin aging.

Experimental Example 16 Hair Loss Prevention Effect Test

Thirty subjects with initial hair loss were tested for hair loss prevention for 16 weeks. After using the composition of Example 2 and Comparative Example 1 once a day for 16 weeks, 16 weeks after the hair was collected again when the hair was collected and counted, the results are shown in Table 17.

Treatment group Fallout parameter number (standard) / standard deviation Comparative Example 1 82.80 / 10.32 Example 2 (Ginseng Flower Extract) 75.07 / 8.70

As shown in the above table, when the composition containing the ginseng flower extract was treated, it was confirmed that the number of the fallen hairs decreased significantly compared to the comparative example.

Experimental Example 17 Inhibitory Effect of Dandruff

In order to determine whether Example 1 (Ginseng flower extract) has an effect of inhibiting dandruff ( P. ovale ATCC 12087), an experiment was performed using a liquid dilution method. At this time, after performing double dilution, and incubated at 32 ℃ for 24 hours and 48 hours, it is determined whether the growth of the bacteria to the extent of suspension to the minimum concentration without MIC (Minimum Inhibition Concentration, minimum inhibition) Concentration). If the mixed solution is opaque and it is difficult to determine the growth of bacteria, it was confirmed by microscopic observation. The results are shown in the table below.

Treatment group MIC (minimum inhibitory concentration) Example 1 (Ginseng Flower Extract) 0.05%

As can be seen from the table, it can be seen that the degree of reduction of MIC of ginseng flower extract is excellent. Therefore, ginseng flower extract can be effectively used for dandruff treatment and prevention.

Experimental Example 18 Hair Gloss Enhancement Effect

After washing 0.1g of the hair sample with 0.1g of shampoo and washing it under running water, the hair sample was dried at 25 ° C. and 50% humidity for 6 hours. Then, 0.1g each of the compositions of Example 2 and Comparative Example 1 of Table 1 was applied, and the gloss was measured using a Goniphotometer in the following manner.

The hair samples were measured for gloss on a coordinator photometer equipped with a halogen lamp. The coherent photometer has an angle of incidence of light of 30 degrees and a reflected value of 0 to 90 degrees can be obtained. Glossiness was calculated by the following equation.

Glossiness (G) = Sf / d

Sf: Specular reflection value, the highest of the reflections

d: numerical value when the reflection is 0 degree

Gloss is a less diffuse reflection and the more the specular reflection occurs, the better the results are shown in Table 19 below.

Treatment group d Sf G Comparative Example 1 6.49 68.41 10.54 Example 2 (Ginseng Flower Extract) 3.58 83.20 23.24

From the results of the table, it was confirmed that the hair gloss significantly improved compared to the comparative example when the composition containing the ginseng flower extract.

Test Example 19 Confirmation of Adipocyte Differentiation Promoting Effect

Human bone marrow mesenchymal stem cells (hBM-MSCs) were purchased from Lonza, Inc. Walkersbille, MD, USA and cultured according to Lonza's instructions. Adipocyte differentiation was performed using a method recommended by Lonza, except that troglitazone (TRO) was used instead of indomethacin to induce adipocyte differentiation of mesenchymal stem cells. Specifically, differentiation of mesenchymal stem cells into adipocytes was performed by incubating 1 g / ml of insulin, dexamethasone (DEXA) 1M, 0.5 mM isobutylmethylxanthine (IBX) and TRO 2M in the culture of mesenchymal stem cells. I was. For the experimental group, the ginseng flower extract, troglitazone, and glibenclamide (purchased from Sigma-aldrich, USA) of Example 1 at the time of inducing adipocyte differentiation were each treated with 10M (treated every two days for 10 days), 10M each was added to the medium. After 10 days of induction of adipocyte differentiation of mesenchymal stem cells, the cell culture medium was removed, the cells were washed with phosphate buffered saline (PBS), and then the cells were washed with 0.2ml / cm ² of 10% formalin / PBS solution. Was fixed for 10 minutes. The fixed cells were washed once with 0.5 ml / cm ² of 60% isopropanol solution, and the lipid body in fat cells was stained with 0.2 ml / cm ² for 10 minutes in a stain solution of Oil Red O in 60% isopropanol. Stained. The stained oil red o was eluted with 100% isopropanol solution and then absorbance was measured at a wavelength of 500 nm. The results are shown in the table below. Each data value was corrected with an IDX (-) negative control.

Treatment group Oil Red Relative Dyeing Ratio Comparative example 1.00 Example 1 (Ginseng Flower Extract) 2.18

As can be seen in the table, the ginseng flower extract was found to promote the differentiation of adipocytes (P value <0.01).

Hereinafter, one example to describe the formulation of the composition according to the present invention, but is not limited thereto.

Formulation Example 1 Flexible Cosmetic (Skin Lotion)

Ingredient Content (% by weight) Purified water Remaining amount Ginseng Flower Extract (Example 1) 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity

Formulation Example 2 Nutritious Longevity (Milk Lotion)

Ingredient Content (% by weight) Purified water Remaining amount Ginseng Flower Extract (Example 1) 0.1 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

EXAMPLE 3 Massage Cream

Ingredient Content (% by weight) Purified water Remaining amount Ginseng Flower Extract (Example 1) 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

Formulation Example 4 Pack

Ingredient Content (% by weight) Purified water Remaining amount Ginseng Flower Extract (Example 1) 0.1 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Pingzi-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, flavoring Quantity

1 is a graph showing the results of the inhibition test of the active oxygen production of ginseng flower extract according to an embodiment of the present invention.

Figure 2 is a graph showing the DNA oxidative damage inhibitory activity of the ginseng flower extract according to an embodiment of the present invention.

Figure 3 is a graph showing the effect of ginseng flower extract according to an embodiment of the present invention on the production of type I procollagen.

Figure 4 is a graph showing the effect of the ginseng flower extract according to an embodiment of the present invention on the expression of MMP-1.

5 is a graph showing the effect of ginseng flower extracts on melanin production according to an embodiment of the present invention.

Claims (29)

An external composition for skin containing ginseng flower extract as an active ingredient. The method of claim 1, The external preparation composition for skin is used for external preparation composition for inhibiting skin damage and protecting the skin. The external preparation composition for skin according to claim 2, wherein the external preparation composition for skin protects the skin through the action of inhibiting skin damage caused by oxidation or ultraviolet rays. According to claim 2, wherein the skin is an external composition for skin, characterized in that the scalp or hair. The external preparation composition for skin according to claim 1, which has a use for preventing or improving skin aging. The method of claim 5, wherein the external composition for skin inhibits aging of the skin through inhibition of skin damage by oxidation or ultraviolet rays, promotion of collagen production or inhibition of biosynthesis of matrix metalloproteinase I (MMP-1). The external preparation composition for skin. The external preparation composition for skin according to claim 1, wherein the external preparation composition for skin has a use for preventing or improving skin wrinkles. The composition for external application for skin according to claim 7, wherein the composition for external application for skin is used to prevent or improve skin wrinkles by promoting collagen production or inhibiting biosynthesis of matrix metalloproteinase I (MMP-1). The external preparation composition for skin according to claim 1, which has a use for improving skin elasticity. The external preparation composition for skin according to claim 1, wherein the external preparation composition for skin has a skin whitening use. The external preparation composition for skin according to claim 10, wherein the external preparation composition for skin is performed by inhibiting melanin production or whitening the pigment deposited on the skin. The external preparation composition for skin according to claim 1, wherein the external preparation composition for skin has a skin moisturizing use. The external preparation composition for skin according to claim 12, wherein the external preparation composition for skin maintains skin moisture by promoting differentiation of keratinocytes or promoting expression of transglutaminase. The external preparation composition for skin according to claim 12, wherein the external preparation composition for skin has a use for preventing or ameliorating a skin dry disease including atopy or psoriasis. The external preparation composition for skin according to claim 1, wherein the external preparation composition for exfoliation has a use of exfoliation. The external preparation composition for skin according to claim 1, wherein the external preparation composition for skin has a sebum control use for skin. The external preparation composition for skin according to claim 16, wherein sebum of the skin is controlled by inhibiting secretion of sebum. The composition for external application for skin according to claim 1, wherein the composition for external application for skin has the purpose of preventing or ameliorating inflammation or alleviating irritation. 19. The composition for external application for skin according to claim 18, wherein the skin is the scalp. The external preparation composition for skin according to claim 1, wherein the external preparation composition for skin has a use for preventing or improving skin troubles including acne. 21. The composition for external application for skin according to claim 20, wherein the skin is the scalp. The external preparation composition for skin according to claim 1, wherein the external preparation composition for skin has a use to improve skin coloration. According to claim 1, wherein the skin is an external composition for skin, characterized in that the scalp or hair. The external preparation composition for skin according to claim 23, wherein the external preparation composition for skin has a use for preventing hair loss. The external preparation composition for skin according to claim 23, wherein the external application composition for skin has antidandruff use. The composition for external application for skin according to claim 23, wherein the composition for external application for skin has a purpose of enhancing hair gloss or softness. The external preparation composition for skin according to claim 1, wherein the external preparation composition for skin has a volume or elasticity promotion purpose of the face or body. The composition for applying the external of the skin according to claim 27, wherein the composition for external application for skin improves the volume and elasticity of the face or body through promoting the differentiation of adipocytes. The external preparation composition for skin according to claim 1, wherein the external preparation composition for skin has a use for improving a face or body line.

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