KR101550917B1 - Composition for applying to skin externally containing extract from seeds of ginseng - Google Patents

Abstract

The present invention relates to the use of ginseng seed extract selected from ginseng seed oil and ginseng seed residue extract. The use of the ginseng seed extract of the present invention can be used for skin protection, anti-aging, anti wrinkle, skin elasticity improvement, whitening, skin moisturizing, Prevention and improvement of diseases, prevention and improvement of skin troubles such as sebum control, anti inflammation and acne, improvement of skin color, exfoliation, hair loss prevention, anti dandruff, improvement of gloss and softness of hair, volume and elasticity of face or body, Includes facial or body line enhancement.

Ginseng seed, ginseng seed oil, extract, protection, aging, wrinkle, elasticity, whitening, moisturizing, atopic, sebum, inflammation, acne, fever, hair, scalp, keratin, volume, line, fat cells, hair loss, anti-dandruff , Polish

Description

[0001] The present invention relates to a composition for external application for skin containing ginseng seed extract,

The present invention relates to the use of ginseng seed extract. More particularly, the present invention relates to the use of ginseng seed extract selected from ginseng seed oil and ginseng seed residue extract. More particularly, the present invention relates to the use of ginseng seed extract for skin protection, anti-aging, anti-wrinkle, skin elasticity improvement, whitening, skin moisturizing, prevention and improvement of skin dryness diseases such as atopy, Anti-inflammatory use, prevention or improvement of skin troubles such as acne, exfoliation, improvement of skin color, prevention of hair loss, anti dandruff, improvement of gloss and softness of hair, volume or elasticity of face or body, improvement of face or body line .

Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogawa and Ginseng. It has been used for over 2,000 years in Korea, China and Japan. It has been empirically used to prevent disease and prolong its life. The known efficacy and efficacy of ginseng are as follows: action on the central nervous system, anticarcinogenic and anticancer activities, immune function regulation, anti-diabetic effect, hyperfunction of liver function, improvement of cardiovascular disorder and anti-arteriosclerosis effect, Anti-stress and anti-fatigue action, antioxidant activity and anti-aging effect are known.

Ginsenoside, a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground of ginseng. However, ginsenoside content and composition vary greatly depending on the site such as ginseng roots, ginseng leaves, and ginseng fruit. In particular, since ginseng seeds were used only as a means to grow ginseng, studies on the oil content of ginseng seeds, especially ginseng seeds, were conducted by analyzing the fatty acid composition and phytosterol content of ginseng seed oil (J. Agric. , 744-750, 2002).

Recently, consumers' interest in natural cosmetics has been increasing, and at the same time, many cosmetics utilizing herbal materials have been released, and ginseng has been studied as a plant material having important skin effect of cosmetics. However, most of them use ginseng roots. Specifically, only the extract of ginseng root or the ginsenoside component of ginseng root and ginseng polysaccharide are used, and there is no research on the skin effect by the ingredient of ginseng seed.

It is an object of the present invention to provide a skin external preparation useful for skin.

It is an object of one embodiment of the present invention to provide a composition for protecting the skin.

It is an object of another embodiment of the present invention to provide a composition for protecting hair.

It is an object of another embodiment of the present invention to provide a composition for preventing or improving aging.

It is an object of another embodiment of the present invention to provide a composition for preventing or improving wrinkles.

Another object of one embodiment of the present invention is to provide a composition for improving skin elasticity.

Another object of one embodiment of the present invention is to provide a composition for skin whitening.

Another object of the present invention is to provide a composition for skin moisturizing.

Another object of the present invention is to provide a composition for preventing or improving skin dryness diseases such as atopy.

Another object of one embodiment of the present invention is to provide a composition for removing keratin.

It is an object of another embodiment of the present invention to provide a composition for controlling skin sebum.

It is an object of another embodiment of the present invention to provide a composition for preventing or alleviating inflammation.

It is an object of another embodiment of the present invention to provide a composition for preventing or alleviating inflammation of the scalp, or alleviating irritation.

Another object of the present invention is to provide a composition for preventing or improving skin troubles such as acne.

Another object of the present invention is to provide a composition for preventing or ameliorating skin troubles such as acne occurring on the scalp.

It is an object of another embodiment of the present invention to provide a composition for improving skin color.

Another object of the present invention is to provide a composition for preventing hair loss.

Another object of one embodiment of the present invention is to provide an anti-dandruff composition.

It is an object of another embodiment of the present invention to provide a composition for promoting gloss or softness of hair.

It is an object of another embodiment of the present invention to provide a composition for enhancing the volume and elasticity of a face or a body.

It is an object of another embodiment of the present invention to provide a composition for improving the face or body line.

To achieve the above object, the composition for external application for skin according to the present invention contains ginseng seed extract as an active ingredient.

In the composition for external application for skin according to an embodiment of the present invention, the ginseng seed extract may be ginseng seed oil. When the ginseng seed extract is ginseng seed oil, the content thereof is preferably 0.001 to 100% by weight based on the total weight of the composition for external application for skin.

In the composition for external application for skin according to another embodiment of the present invention, the ginseng seed extract may be a ginseng seed residue extract. The content of the ginseng seed residue extract is preferably 0.001 to 10% by weight based on the total weight of the composition for external application for skin.

The composition for external application for skin according to one embodiment of the present invention is characterized by having a use for protecting the skin by inhibiting skin damage. Wherein the skin comprises scalp or hair. That is, in the present specification, a composition for external application for skin which protects the hair by suppressing damage to the scalp or hair is disclosed.

The composition for external application for skin according to another embodiment of the present invention is characterized by protecting the skin through an action of suppressing skin damage caused by oxidation or ultraviolet rays. Wherein the skin comprises scalp or hair. That is, a composition for external application for skin which protects scalp or hair by inhibiting damage of scalp or hair by oxidation or ultraviolet rays is disclosed.

The composition for external application for skin according to another embodiment of the present invention is characterized by having a use for preventing or improving skin aging.

The composition for external application for skin according to another embodiment of the present invention inhibits skin aging through inhibition of skin damage by oxidation or ultraviolet rays, promotion of collagen production, or inhibition of biosynthesis of matrix metalloproteinase I (MMP-1) .

The composition for external application for skin according to another embodiment of the present invention is characterized by having an application for preventing or improving skin wrinkles.

The composition for external application for skin according to another embodiment of the present invention is characterized by preventing or improving wrinkles of the skin through promotion of collagen production or inhibition of biosynthesis of matrix metalloproteinase I (MMP-1).

The composition for external application for skin according to another embodiment of the present invention is characterized by having an application for improving skin elasticity.

The composition for external application for skin according to another embodiment of the present invention is characterized by having skin whitening application.

The composition for external application for skin according to another embodiment of the present invention is characterized by performing skin whitening through inhibition of melanin formation or whitening of pigment deposited on the skin.

The composition for external application for skin according to another embodiment of the present invention is characterized by having a skin moisturizing use.

The composition for external application for skin according to another embodiment of the present invention is characterized in that skin moisture is maintained through promotion of differentiation of keratinocytes or promotion of transglutaminase expression.

The composition for external application for skin according to another embodiment of the present invention is characterized by having a use for preventing or ameliorating skin dryness diseases including atopic or psoriasis.

In another embodiment of the present invention, the composition for external application for skin is characterized by having an application for exfoliation.

The composition for external application for skin according to another embodiment of the present invention is characterized by having a skin sebum control purpose.

The composition for external application for skin according to another embodiment of the present invention is characterized by controlling sebum of the skin by inhibiting secretion of sebum.

The composition for external application for skin according to another embodiment of the present invention is characterized by having the use of preventing or ameliorating inflammation or relieving irritation. Here the skin contains the scalp. That is, in the present specification, there is provided a composition for external application for skin having the use of preventing or ameliorating inflammation of the scalp and alleviating irritation to the scalp.

The composition for external application for skin according to another embodiment of the present invention is characterized by having a use for preventing or improving skin troubles including acne. Here, the skin contains the scalp. That is, in the present specification, there is provided a composition for external application for skin having a use for preventing skin troubles including acne on the scalp from occurring and for improving skin troubles that have already occurred.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for improving skin color.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for preventing hair loss.

In another embodiment of the present invention, the composition for external application for skin is characterized by having anti-dandruff application.

In another embodiment of the present invention, the composition for external application for skin is characterized in that it has a use for improving gloss or softness of hair.

In another embodiment of the present invention, the composition for external application for skin is characterized in that it has a use for increasing the volume and elasticity of a face or a body.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for improving lines of a face or a body.

In the present invention, ginseng root extract and ginseng seed extract having different composition and composition from ginseng root are contained as an effective ingredient in the top part of ginseng to provide skin protection, anti-aging, anti wrinkle, skin elasticity improvement, whitening, skin moisturizing, Prevention and improvement of skin, prevention and improvement of skin trouble such as sebum, anti-inflammation and acne, exfoliation, improvement of skin color, prevention of hair loss, anti dandruff, improvement of gloss and softness of hair, volume and elasticity of face or body, Or a composition for improving the line of the body can be provided.

As used herein, the term "extract" refers to a substance obtained by extracting a component contained therein from a natural product, regardless of the method of extraction or the kind of the component. For example, it is a broad concept including both extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and extracts of specific components of natural products, such as oils, and extracts of specific components.

In the present specification, the term "ginseng seed oil" means an oil obtained from the seeds of ginseng. For example, it may be an extract extracted from ginseng seed using an organic solvent such as hexane, ether, methylene chloride or the like.

In the present specification, the term "ginseng seeds" means the remaining portion of the ginseng seed which has been removed from the oil. For example, an organic solvent such as hexane, ether, or methylene chloride may be used to remove remaining oil from the ginseng seed.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is a concept of the broadest notion including a scalp and hair as well as a tissue covering a body surface such as a face or a body.

In an embodiment of the present invention, the ginseng is Panax ginseng C. A. Meyer.

In the composition for external application for skin according to an embodiment of the present invention, the ginseng seed residue extract may be mixed in an amount of 0.001 to 10% by weight based on the total weight of the composition, depending on the form of the composition for external application for skin. If the content of ginseng seed residue is less than 0.001% by weight, the effect is insignificant. If the content exceeds 10% by weight, the effect is not increased more than the content.

The ginseng seed residue extract can be prepared by the following method. Dried ginseng seeds are pulverized, and then 1 to 10 times (wt / v%) of an organic solvent such as hexane is added and left at room temperature for one day to be extracted. The above process is repeated three times to remove the oil from the ginseng seed and the organic solvent (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.) 1 to 10 times (wt / v%) of solvent may be added and left at room temperature for one day to be extracted. The above process is repeated three times to obtain an extract. The extract is filtered, and the filtrate is concentrated in a vacuum concentrator to obtain a dry product of ginseng seed residue.

In another embodiment of the present invention, the ginseng seed extract is ginseng seed oil, and the oil may be mixed in an amount of 0.001 to 100 wt% based on the total weight of the composition, depending on the form of the composition for external application for skin. If the ginseng seed oil is less than 0.001% by weight, the effect is insignificant.

In another embodiment of the present invention, the ginseng seed oil is prepared by pulverizing dried ginseng seeds, and then subjecting the ginseng seed oil to an organic solvent (hexane, ether, methylene chloride, etc.) And allowed to stand at room temperature for one day. For example, the above process is repeated three times to obtain an extract, and the extract is filtered, and the filtrate is concentrated in a vacuum concentrator to obtain ginseng seed oil.

The human skin undergoes changes due to various internal and external factors as it gets older. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction As the amount of ultraviolet rays reaching the surface of the sunlight increases and the environmental pollution is further intensified, free radicals and active noxious oxygen are increased, thereby decreasing the thickness of the skin, increasing the wrinkles and decreasing the elasticity However, skin coloration becomes dull and unpleasant, skin troubles frequently occur, and spots, freckles, and black blotch also increase.

As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid, and glycoprotein, which constitute the skin, change or decrease, and oxidative stress due to free radicals and active noxious oxygen occurs. Cox-2 (cyclooxygenase), an enzyme that produces a proinflammatory cytokine, known to cause inflammation in most cells constituting the skin by aging or ultraviolet rays, And the biosynthesis of Matrix metalloproteinase (MMP), an enzyme that degrades skin tissue, is increased by these inflammatory factors, and nitric oxide (NO) production by iNOS (inducible nitric oxide synthase) Respectively. In other words, decrease of cellular activity due to natural endogenous aging and decrease of biosynthesis of substrate material by microinflammation, increase of stress due to various harmful environment, increase of active oxygen species by sunlight, As the degradation and denaturation are accelerated, the skin substrate is destroyed and thinned, and various symptoms of skin aging appear.

The composition for external application for skin disclosed in the present specification contains a ginseng seed extract having an effect of inhibiting the production of active oxygen and inhibiting oxidative damage caused by active oxygen as an active ingredient to thereby inhibit cell damage by active oxygen or ultraviolet rays It can prevent and protect the skin through this. Further, progress of aging by active oxygen or ultraviolet rays can be suppressed. In addition, the ginseng seed extract can prevent or ameliorate inflammation by inhibiting the biosynthesis of COX-2, thereby alleviating skin troubles. Here the skin contains the scalp. That is, the ginseng seed extract disclosed in the present specification can prevent damage to cells caused by active oxygen or ultraviolet rays generated from the scalp or hair, prevent or improve inflammation of the scalp, relieve the stimulation of the scalp by perming, It can be used for applications. In addition, the ginseng seed extract disclosed in the present specification can also be used for preventing or improving wrinkles of the skin.

Several factors are involved in determining the skin color of a person. Among them, factors such as the activity of the melanocyte making the melanin pigment, the distribution of the blood vessels, the thickness of the skin, the presence or absence of pigment in and out of the human body such as carotenoid and bilirubin This is important.

Especially, the most important factor is melanin, a melanin pigment produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside of the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results.

The ginseng seed extract disclosed in the present specification can whiten skin by inhibiting the production of melanin. Accordingly, the composition for external application for skin disclosed in the present specification, which contains ginseng seed extract as an active ingredient, can be used for skin whitening purposes.

The most important function of the epidermis in the outermost skin is to protect against external stimuli (chemical, air pollutants, dry environment, physical and chemical stimulants such as ultraviolet rays) This protective function can maintain its function only when the stratum corneum composed of keratinocytes is normally formed. The outermost stratum corneum (horney layer) is formed from keratinocytes and consists of keratinocytes with complete differentiation and a surrounding lipid layer. The keratinocyte is a characteristic cell in which basal cells continuously proliferating in the lowest epidermis undergo a stepwise change in morphology and function and are elevated to the surface of the skin. After a certain period of time, And the new keratinocyte replaces its function. This repetitive sequence of changes is called "epidermal cell differentiation" or "keratinization". During keratinization, keratinocytes form the stratum corneum while producing natural moisturizing factors (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids), which make the stratum corneum firm and flexible, As shown in Fig.

However, such a stratum corneum can be easily lost due to excessive harshness, environmental factors such as living habits such as bath, dry atmosphere, pollutants, and endogenous diseases such as atopic skin and senile skin, In fact, due to the increasing number of factors in the modern age, there has been an increasing tendency in recent years to appeal to people with dry skin symptoms and disorders. Therefore, in order to maintain proper skin moisture, studies have been conducted to supply moisture from the outside or to prevent water loss from the body, and a variety of moisturizers having moisture retention ability have been developed, It is widely used in the area.

However, as the risk factors for humans increase in the living environment and the aging population rapidly increases, the turnover rate of the stratum corneum is delayed, the lipid synthesis ability of the keratinocytes decreases, The cells are not smoothly divided, maturated and differentiated, and the amount of moisturizing factors and lipids in the stratum corneum is decreased, so that the normal stratum corneum does not maintain its function. Namely, .

Due to the division and differentiation of abnormal abnormal epidermal cells, the skin causes various skin diseases such as dry skin, atopy and psoriasis. These diseases can alleviate the symptoms only by a conventional moisturizing agent having only moisture holding function , But it is difficult to expect fundamental healing.

The ginseng seed extract disclosed in this specification promotes the differentiation of keratinocytes and thus helps the process of elimination of old keratinocytes and the process of "differentiation of epidermal cells" or "keratinization", which is a process of generating new keratinocytes, Can effectively be removed. The composition for external application for skin disclosed in this specification can be effectively used as a composition for removing exfoliation by containing an extract of ginseng seed having an excellent exfoliation effect as an effective ingredient as described above.

In addition, the ginseng seed extract disclosed in the present specification can keep the amount of moisturizing factor and lipid in the stratum corneum normally by keeping the turnover rate of the stratum corneum at a normal range. It also promotes the expression of transglutaminase. Through this, it is possible to moisturize the skin.

In addition, the ginseng seed extract disclosed in the present specification can promote the differentiation of keratinocytes and enhance skin barrier function by maintaining skin moisture, thereby preventing or treating skin dryness diseases such as atopy and psoriasis.

In general, sebaceous glands in the skin, including the scalp and face, play a role in preventing moisturization and microbial invasion of the skin. However, when excess sebum secreted by sebum is secreted excessively, hair loss is promoted and acne is worsened. And seborrheic dermatitis is caused.

The hypersecretion of sebaceous glands is caused by various causes, and the most important of them is the activation of sebaceous glands by the amount of dihydrotestosterone (DHT), one of the hormones involved in promoting sebaceous gland secretion in sebaceous gland activity, It is overdone. That is, in the case of hair loss, testosterone (T) is converted into dihydrotestosterone, one of the male hormones, by the 5-alpha-reductase type 2 in cells, It binds to the receptor to cause hair loss. However, in skin and sebaceous glands, testosterone is converted to dihydrotestosterone by 5-α-reductase type 1 to activate sebaceous cells By accelerating the differentiation sebum in the sebaceous (sebum) is overdose to cause acne.

Acne and hair loss, which are skin troubles other than simple sebum hypersecretion, are further exacerbated by the minute inflammatory reaction of the skin. The cause of acne is that excess sebum accumulates in the hair follicle, activating acne activity and inducing inflammation.

The ginseng seed extract disclosed in the present specification can prevent or improve acne, enlargement of pores and seborrheic dermatitis due to excessive secretion of sebum by inhibiting sebum secretion.

The composition for external application for skin disclosed in the present specification can promote volume and elasticity of face and body by promoting differentiation of adipocytes. Applying the composition for external application for skin disclosed herein to areas where the volume and elasticity of the face and the body are required to be improved, volume and elasticity are enhanced. The area where volume and elasticity are required to be promoted may be a depressed area due to aging. For example, it may be a bow tortured by aging. Applying the composition for external application for skin disclosed herein to a depressed ball may promote volume by promoting adipocyte differentiation. Further, as another example in which the volume and the elasticity are required to be enhanced, a chest and a hip region are exemplified. When the composition for external application for skin disclosed in this specification is applied to the chest and the buttocks, the chest and buttocks are resilient and bulky.

In addition, the composition for external application for skin disclosed in this specification can improve the line of face and body. The ginseng seed extract contained as an active ingredient promotes the differentiation of adipocytes, thereby enhancing the volume and elasticity of the face and body, promoting collagen biosynthesis, inhibiting collagen degradation, and preventing the damaged face and body Can be improved.

The cosmetic form according to the invention contains a cosmetically or dermatologically acceptable medium or base. It may be in any form suitable for topical application, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing the oil phase in water, a suspension, a microemulsion, a microcapsule, In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art. The composition according to the invention may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition of the present invention can be used in the form of a cosmetic or dermatological composition comprising a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, , Cosmetics such as fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants conventionally used in the field of dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

The external preparation for skin including ginseng seed extract of the present invention is not particularly limited in its formulation. Examples of the external preparation for skin include softening lotion, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, Cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil and body essence.

The composition for external application for skin according to the present invention may be a pharmaceutical composition. The pharmaceutical composition may additionally contain preservatives, stabilizers, wetting or emulsifying accelerators, pharmaceutical adjuvants such as salts and / or buffers for controlling osmotic pressure, and other therapeutically useful substances. The pharmaceutical composition may be formulated into lotions, creams, ointments or gels.

The pharmaceutical composition is preferably transdermally administered.

The dosage of ginseng seed extract, which is an effective ingredient of the pharmaceutical composition, will depend on the age, sex, and weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, . Dosage determinations based on these factors are within the level of those skilled in the art. Generally, the dosage ranges from 0.0001 mg / kg / day to about 2000 g / kg / day.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to examples and test examples. However, these examples and examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Example 1] Production of ginseng seed oil

1 kg of dried ginseng seeds were pulverized and then 10 L of hexane was added thereto, and the mixture was allowed to stand at room temperature for one day. The above process was repeated three times to obtain an extract. The extract was filtered, and the filtrate was concentrated in a vacuum concentrator to obtain 120 g of ginseng seed oil. Sodium hydroxide was added to the extract oil to remove the free fatty acids. The extract was washed three times with distilled water, decolorized with carbon, and then removed with an inert gas under a vacuum of 100 ° C to obtain 100 g of ginseng seed oil.

[Example 2] Preparation of ginseng seed residue extract

1 kg of dried ginseng seeds were pulverized and then 10 L of hexane was added thereto, and the mixture was allowed to stand at room temperature for one day. The above process was repeated 3 times to remove the oil from the ginseng seeds, and 10 L of water was added to the remaining ginseng seeds after the oil was removed, and the mixture was left standing at room temperature for one day. The above process was repeated three times to obtain an extract. The extract was filtered, and the filtrate was concentrated in a vacuum concentrator to obtain 100 g of dry solid.

[Experimental Example 1] Inhibition of active oxygen production

Antioxidant effect was investigated by comparing the ability to remove reactive oxygen species (ROS) from cells by ultraviolet (UV) irradiation. As a positive control, trolox (Aldrich Co.), which is generally used to compare antioxidative effects, was used. In addition, the test group was not used as a control group. (UVB 30 mJ / cm ² ) was applied to a human HaCaT keratinocyte monolayer culture system (P Boukamp, German Cancer Research Center, Heidelberg. German) Oil and the ginseng seed residue extract of Example 2 are shown in Table 1 below Respectively. Then, the amount of ROS was measured with a fluorescent plate reader using DCFH-DA (2 ', 7'-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc.). The ratio of inhibition of ROS formation was calculated as a ratio to the control group treated with ultraviolet light. The results are shown in Table 1.

Test substance ROS generation inhibition rate (%) Control group 0 Trolox 0.1 mM 42.7 Example 1 (10 ppm) 52.9 Example 1 (1 ppm) 38.3 Example 2 (10 ppm) 58.1 Example 2 (1 ppm) 45.9

The ginseng seed oil prepared according to Example 1 and the ginseng seed extract of Example 2 showed an increase in free oxygen generated by UV in the human HaCaT keratinocytes monolayer culture system (human HaCaT keratinocytes monolayer culture system) The effect of erasing is shown. This is an excellent activity similar to that of trolox used as an activity index of antioxidants. That is, the ginseng seed oil produced according to Example 1 of the present invention and the ginseng seed residue extract of Example 2 significantly inhibited active oxygen which is a cause of skin damage, thereby protecting the skin including scalp and hair, Further, it has been confirmed that it is possible to prevent wrinkle formation, decrease in elasticity and pigmentation.

[Experimental Example 2] Inhibitory activity against DNA oxidative damage by single cell gel electrophoresis (SCGE, Comet assay)

Human normal fibroblasts (their primary cultures) were cultured for 24 hours. Then, the ginseng seed oil prepared according to Example 1, the ginseng seed extract of Example 2, and vitamin E were treated for each concentration. After 12 hours, H ₂ O ₂ (final concentration: 10 -3 M), and then stained with electrophoresis. The result was observed with a fluorescence microscope. At this time, the length (um) of the tail, which is the result of DNA cleavage in each of 25 cells, was analyzed using an image analyzer, KOMET 3.1 (Kinetic Imaging, England) Respectively.

Test substance Tail length (탆) Inhibition rate (%) Control group 121.1 0.0 Example 1 (1 ppm) 76.2 37.1 Example 1 (10 ppm) 68.8 43.2 Example 2 (1 ppm) 88.5 26.9 Example 2 (10 ppm) 72.9 39.8 Vitamin E 10 ppm 68.9 43.1

In the results of Table 2, the ginseng seed oil produced by the cell gel electrophoresis according to Example 1 and the ginseng seed residue extract of Example 2 showed significant oxidative DNA damage inhibitory activity, (Ginseng Seed Extract) as a DNA oxidative damage inhibitor which inhibits DNA single strand break by hydroxyl radical (.OH). This is an important protective effect for protecting the genes of cells of the skin including the scalp or hair.

[Experimental Example 3] Type I procollagen assay (type I procollagen assay)

The human fibroblasts (their primary cultures) were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , and then the ginseng seed oil prepared according to Example 1 and the ginseng seed residue extract of Example 2 were cultured in a medium Respectively. On the third day of culture, the cells were harvested and the amount of type I procollagen produced by ELISA was quantitated. The results are shown in Table 1. The results are shown in Table 1 and Table 2. The results are shown in Table 1 and Table 2. The positive control group was TGF-b (Human Transforming Growth Factor-? 1, Roche Co.) Were used.

Type I Procollagen Biosynthesis (%) Negative control group 0 Example 1 (1 ppm) 19 Example 1 (10 ppm) 39 Example 2 (1 ppm) 11 Example 2 (10 ppm) 25 TGF-b 10 ng / ml 100

In the normal human fibroblast monolayer culture system, the ginseng seed oil of Example 1 and the ginseng seed residue extract of Example 2 significantly enhanced the production of type I procollagen as compared with the control. That is, the composition for external application for skin containing ginseng seed extract according to the present invention promotes collagen production, thereby alleviating the collagen reduction phenomenon caused by aging of human skin.

[Experimental Example 4] Effect of improving skin wrinkles on human skin

For 80 subjects who had facial wrinkles of 30-50 years old, the following Table 4 shows that the ginseng seed oil of Example 1 and Example 3 containing the ginseng seed residue extract of Example 2 and the ginseng seed extract of Example 2 containing no ginseng seed extract The nutritional creams of Comparative Example 1 were prepared and their skin wrinkle-improving effects were comparatively evaluated (unit: wt%).

ingredient Example 3 Comparative Example 1 Ginseng seed oil 0.1 - Ginseng seed residue extract 0.1 - Wax 10 10 Polysorbate 60 1.5 1.5 Piggy 60 hardened castor oil 2 2 Sorbitan sesquioleate 0.5 0.5 Liquid paraffin 10 10 Squalane 5 5 Caprylic / capric triglyceride
(Estasan: Uniqema (manufacturer)) 5 5 glycerin 5 5 Butylene glycol 3 3 Propylene glycol 3 3 Triethanolamine 0.2 0.2 Preservative, coloring, fragrance Suitable amount Suitable amount Purified water Balance Balance

20 subjects were recruited and the subjects were used in Example 3 for the facial seat and for Comparative Example 1 for 3 months. The skin condition of both sides of the facial area before the use of the cream was measured, and then the same part was remeasured 3 months after using the cream to measure the change of the skin wrinkles. The skin wrinkles were measured with a Visiometer system (C + K company) by removing the wrinkles of the eye tail region with a replica in a constant temperature and humidity chamber at 24 ° C and a relative humidity of 40%. The amount of change in the skin wrinkles was calculated according to the following formula (1).

In the equation (1), "Tdi" is the measured site value at D90 and "Tdo" is the measured site value at D0.

As a result of calculation according to the above formula (1), skin wrinkles at the site using Comparative Example 1 showed a decrease of 6.1 +/- 7% (mean 占 standard deviation), while skin wrinkles at the site using Example 3 were 32 占And 5%, respectively.

[Experimental Example 5] Improvement of skin elasticity in human body

The skin elasticity improving effect of Example 3 and Comparative Example 1 prepared in the composition of Table 4 was measured. The nutritional creams of Example 3 and Comparative Example 1 were applied to the face of 20 women over 30 years old twice a day for 12 weeks, and then skin elasticity meter (Cutometer SEM 575, C + K Electronic Co., Germany) was used And skin elasticity was measured. As a result, the elasticity effect of the region using Comparative Example 1 was 0.21 ± 0.14 (mean ± standard deviation), while the elasticity effect of the region using Example 3 was 0.29 ± 0.08, Respectively. The resultant value indicates a property of skin viscoelasticity of the skin elasticity measuring device.

[Experimental Example 6] Inhibitory effect of melanin on mouse pigment cells

Mouse pigment cells were used to examine melanin production inhibitory effects of the ginseng seed oil of Example 1 and the ginseng seed residue extract of Example 2.

First, 10% fetal bovine serum, 100 nM (100 nM) of Dulbecco's modified Eagles media was injected into DMEM (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) 2-O-tetradecanoyphorbol-13-acetate and 1 nM cholera toxin at 37 ° C and 5% CO ₂ . The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, and cells were cultured at a concentration of 10 ⁵ cells / well in a 24-well plate. ppm and 100 ppm ginseng seed oil (Example 1) and ginseng seed residue extract (Example 2) were added and cultured. At this time, arbutin (Sigma Co.) was used as a positive control. Then, the culture solution was removed, washed with PBS, and the cells were dissolved with 1 N sodium hydroxide. Absorbance was measured at 400 nm, and the inhibition rate of melanin production was calculated according to the following equation (2) (Dooley's method).

Melanin production inhibition rate (%) Example 1 Example 2 Untreated group 0 0 10 ppm treated group 3 23 50 ppm treated group 19 32 100 ppm treated group 43 51 Arbutin 100 ppm 51

As shown in Table 5 and FIG. 1, the ginseng seed oil of Example 1 according to the present invention and the ginseng seed residue extract of Example 2 showed a similar inhibition rate of melanin formation to that of arbutin used as a control group. Thus, it was confirmed that the ginseng seed oil of Example 1 and the ginseng seed residue extract of Example 2 according to the present invention showed excellent whitening effect.

[Experimental Example 7] Whitening effect on human skin

The following experiment was conducted to examine the whitening effect of the ginseng seed oil of Example 1 and the ginseng seed residue extract of Example 2 on human skin.

First, 12 healthy boys were exposed to a 1.5 cm diameter opaque tape at the upper part of the subject's body, and then they were exposed to ultraviolet rays (UVB) of about 1.5 to 2 times the minimum erythema dose of each subject. To induce blackening of the skin.

The ginseng seed oil of Example 1, the ginseng seed residue extract of Example 2, and hydroquinone, which were the test substances after the ultraviolet ray irradiation, were applied, and the state change was observed for 10 weeks without applying any one of them. The color of skin was measured with a colorimeter CR2002 (Minolta Co., Japan) every 1 week.

Then, the difference (ΔL *) between the start time of application of the respective test materials and the completion time of the application of the test materials was calculated according to the following equation (3), and the results are shown in Table 7 below. On the other hand, the whitening effect is judged by comparison of DELTA L * between the sample application site and the control site. When the DELTA L * value is 2, the whitening of the deposited pigment is noticeable. .

DELTA L * = L * value at the time of completion of application - L * value at the start of application

Test substance The degree of skin color brightness (DELTA L *) Example 1 1.94 + 0.11 Example 2 1.91 + 0.14 Hydroquinone (positive control) 1.89 ± 0.15 Vehicle (negative control) 0.51 + - 0.13

As shown in Table 6, it was confirmed that the ginseng seed oil of Example 1 and the ginseng seed residue extract of Example 2 according to the present invention exhibited skin color brightness similar to that of hydroquinone. This is because it improves pigmentation caused by ultraviolet light and brightens skin color.

[Experimental Example 8] Differentiation induction of human keratinocytes

The amount of CE (cornified envelope) produced during the differentiation of keratinocyte and human skin cell line (HaCaT) was measured to promote the cell differentiation of the ginseng seed oil of Example 1 and the ginseng seed residue extract of Example 2 .

The primary cultured human keratinocytes (self-primed) were put in a culture flask and adhered to the bottom. The test substances described in Table 7 below were added to the culture medium by the concentration, and the cells were incubated at 70 to 80% Lt; / RTI > for 5 days. The cells were harvested and washed with PBS (phosphate buffered saline). Then, the cells were washed with 10 mM Tris-HCl buffer (Tris-HCl buffer containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) , pH 7.4), sonication, boiling, and centrifugation were suspended again in 1 ml of PBS, and the absorbance at 340 nm was measured. Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a criterion when evaluating the degree of cell differentiation. The test results are shown in Table 7 below, in which low calcium (0.03 mM) treated group and high calcium (1.2 mM) treated group are negative / positive control group, and low calcium concentration test substance is added.

Test substance The ability to differentiate in keratinocytes (%) Control group The low calcium treatment group (Ca ²⁺ 0.03 mM) 100 The calcium treatment group (Ca ²⁺ 1.2 mM) 213 Example 1 1 ppm 111 10 ppm 119 100 ppm 137 Example 2 1 ppm 97 10 ppm 109 100 ppm 121

From the results of Table 7, it was confirmed that the ginseng seed oil of Example 1 and the ginseng seed residue extract of Example 2 according to the present invention promote differentiation in keratinocytes. This indicates that the elimination of old keratinocytes and normalization of turnover cycle, which is a generation cycle of new keratinocyte, can enhance skin moisturization. Also, it was confirmed that ginseng seed extract can promote skin exfoliation.

[Experimental Example 9] Increase in skin moisturizing power in human body

Fifty male and female adult men and women representing dry skin were divided into two groups, and Example 3 and Comparative Example 1 were applied twice daily to the face for 4 weeks in each group. (24 ° C, relative humidity: 40%) after one week, two weeks, and four weeks after application and before two weeks (six weeks after completion of application) Skin moisture was measured and the results are shown in Table 8 below. The test result is a percentage of the increment of the measured value after a certain period of treatment based on the coneometer value measured just before the start of the test.

Test substance Moisture growth rate (%) 1 week Two weeks 4 weeks 6 weeks Example 3 34 40 42 29 Comparative Example 1 30 34 34 15

Connio meter is a skin moisture analyzer that measures the amount of water present in the skin by measuring the electrical conductivity of the skin. As shown in Table 8, in the group to which the substance of Example 3 containing the ginseng seed oil of Example 1 and the ginseng seed oil extract of Example 2 were applied, the skin moisture content was higher than that of the control group to which Comparative Example 1 was applied Respectively. Further, the values of the skin moisture measured 2 weeks after the stop of application of the nutritional cream of Example 3 (total of 6 weeks) were similar to those after 1 to 2 weeks, It was found that the skin moisture was continuously maintained during application of the ginseng seed oil extract and ginseng seed residue extract according to the present invention.

[Experimental Example 10] Effect of suppressing sebum secretion

In order to measure the oily skin mitigation effect, 20-45 year-old female layer was applied to 20 people with oily skin, 10 persons to Example 3 (containing ginseng seed oil and ginseng seed oil extract) 1 was applied twice a day in the morning and evening. After 1 week, 2 weeks, 3 weeks and 4 weeks, sebum secretion amount of skin was measured using a sebum smear meter (Sebumeter SM810). The experiment was carried out at 20 ° C and 20% relative humidity. The measurement results are shown in Table 9. The results were expressed as the average value of 10 subjects. In the case of oily skin, the measurement value is 220 or more, and in the case of normal skin, it is between 100 and 220.

Test substance Before application 1 week 2 weeks 3 weeks 4 weeks Comparative Example 1 246 240 239 235 230 Example 3 244 235 231 217 211

From the results of Table 9, it can be seen that the nutritional cream of Example 3 containing ginseng seed oil according to the present invention has an effect of suppressing excessive sebum secretion and has a sebum control effect. It is possible to prevent acne and trouble caused by excessive sebum occurring in the skin including the scalp.

EXPERIMENTAL EXAMPLE 11 Effect of LPS (Lipopolysaccharide) Induced Cyclooxygenase-2 (COX-2) on Biosynthesis

Human fibroblasts (their primary cultures) were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , and then subjected to LPS treatment. Then, 1 ppm of the ginseng seed oil of Example 1 and 50 ppm of the ginseng seed oil extract of Example 2 Containing medium. On the second day of culture, cells were harvested and the amount of cyclooxygenase-2 (COX-2) produced using the Westren blot method was quantitated. The comparison with the value measured with a densitometer with a control value of 100 is shown in Table 10 below. The control group was an untreated group in which the ginseng seed oil of Example 1 was cultured without treatment.

Test substance (concentration: ppm) COX-2 biosynthesis (%) Example 1 50 42 One 78 Example 2
50 62 One 89 Control group 100

As can be seen from the results of Table 10, the ginseng seed oil of Example 1 and the ginseng seed extract of Example 2 according to the present invention showed the biosynthesis of cyclooxygenase-2 induced by LPS, which is an inflammatory reaction inducer, Dependent reduction of inflammation, thereby suppressing the inflammatory reaction, thus showing the effect of relieving skin troubles.

 [Experimental Example 12] Effect of improving acne and skin troubles

The subjects were 10 women with severe skin troubles including acne. The subjects were soaked in soap for 30 minutes in the temperature and humidity chamber, and the skin trouble was measured by comparing the number of acne and inflammation .

The subjects were allowed to use the nutritional creams of Example 3 and Comparative Example 1 for 4 weeks, respectively, and the degree of acne and skin troubles were compared. The results are shown in Table 11 below.

Example 1
(5 people) Comparative Example 1
(5 people) Skin Trouble Improvement Ratio 84% 32%

As can be seen from the results of Table 11, when the ginseng seed oil of Example 1 according to the present invention and the ginseng seed oil extract of Example 2 were applied for 4 weeks, the number of existing acne was decreased and the inflammation accompanied The improvement of the trouble appeared.

[Experimental Example 13] Improvement in color of human skin (face)

In order to evaluate the effect of accelerating the blood circulation in the skin of the composition containing the ginseng seed extract of the present invention, the degree of blood circulation in the skin was measured using LDPI (Laser Doppler Perfusion Imager). LDPI is a widely used and widely used device for measuring blood circulation in the skin. It is a highly sensitive device that can measure not only the velocity and quantity of blood in the capillaries of the skin but also the flow in the small arteries and the parenchyma .

The face was soaked with soap and allowed to adjust for 30 minutes. The initial value was measured using an IR (Infrad) camera with LDPI and a skin temperature measuring instrument. The initial blood flow in the lower part of the forehead was measured by LDPI and the initial temperature of the forehead, under the eyes and cheeks was measured using an IR camera.

Blood flow and skin temperature (占 폚) were compared with the initial measured values after using Example 3 for a week, and the results are shown in Tables 14 and 15, respectively.

Face Before use After 1 week use medium 0.945 1.154

forehead Under the eyes cheek Before use (℃) 32.16 32.09 30.34 After 1 week use (℃) 33.67 33.81 33.75

As described above, Example 3 containing the ginseng seed oil extract and ginseng seed residue extract according to the present invention was used, and it was confirmed that the color tone was improved due to the peripheral blood circulation improving effect on the faces of the subjects, It is suggested that the ginseng seed extract according to the present invention can contribute to effectively transmit nutrients of the skin and inhibit and retard skin aging.

[Examples 3 and 4, Comparative Example 2]

The compositions containing ginseng seed oil and ginseng seed residue extracts prepared in Examples 1 and 2 were mixed according to the composition shown in Table 16 below.

Component (% by weight) Example 3 Example 4 Comparative Example 2 Ginseng seed oil (Example 1) 10 - - Ginseng seed residue extract (Example 2) - 0.1 - Caprylic Capric Triglyceride 70 70 80 ethanol 10 10 10 water Balance Balance Balance

[Experimental Example 14] Test for preventing hair loss

A study on the effect of preventing hair loss was conducted on 30 subjects who had an early hair loss phenomenon for 16 weeks. The compositions of Examples 3 and 4 and Comparative Example 2 were used once a day for 16 weeks. After 16 weeks, the hair was collected again and hair was collected again, and the number was counted. The results are shown in Table 17 .

Treated group Number of dropouts (average) / standard deviation Comparative Example 1 82.80 / 10.32 Example 3 (ginseng seed oil) 76.27 / 9.10 Example 4 (Ginseng seed residue extract) 79.21 / 6.10

As shown in the table, when the composition containing ginseng seed oil or ginseng seed residue extract was treated, it was confirmed that the number of decolorization moths was significantly reduced as compared with the comparative example.

[Experimental Example 15] Experiments for inhibiting dandruff

To investigate the effect of Example 1 (ginseng seed oil) and Example 2 (ginseng seed residue extract) on inhibition of dandruff ( P. ovale ATCC 12087), experiments were conducted using a broth dilution method Respectively. After double dilution, the cells were cultured at 32 ° C for 24 hours and 48 hours, and the presence or absence of bacterial growth was judged by the degree of suspension. The minimum concentration without bacterial growth was defined as Minimum Inhibition Concentration (MIC) Concentration). If the mixture is opaque and it is difficult to judge the growth of bacteria, it is confirmed by microscopic observation. The results are shown in the following table.

Treated group MIC (minimum inhibitory concentration) Example 1 (ginseng seed oil) 0.04% Example 2 (Ginseng seed residue extract) 0.07%

As can be seen from the above table, it can be seen that the degree of reduction of MIC of ginseng seed oil and ginseng seed residue extract is excellent. Therefore, ginseng seed extract can be effectively used for treating and preventing dandruff.

[Experimental Example 16] Hair gloss enhancement effect

0.1 g of the hair sample was washed with 0.1 g of shampoo, washed thoroughly with flowing water, and then the hair sample was dried at 25 ° C and 50% humidity for 6 hours. Then, 0.1 g each of the compositions of Examples 3 and 4 and Comparative Example 2 in Table 18 were applied, and the gloss was measured using a cone photometer in the following manner.

The hair samples were measured for gloss in a cone photometer equipped with a halogen lamp. The cone photometer has a light incident angle of 30 degrees and can obtain a reflected value from 0 to 90 degrees. The gloss was calculated by the following equation.

Glossiness (G) = Sf / d

Sf: the highest value among reflection values by specular reflection value

d: When the diffuse reflection value is 0 degree

The gloss is less diffuse and the more the specular reflection occurs, the better the result. The measurement results are shown in Table 19 below.

Treated group d Sf G Comparative Example 1 6.49 68.41 10.54 Example 3 (ginseng seed oil) 2.81 87.45 31.12 Example 4 (Ginseng seed residue extract) 3.91 64.23 16.43

From the results of the above table, it was confirmed that when the composition containing ginseng seed extract was treated, the hair gloss was significantly improved as compared with the comparative example.

[Test Example 17] Confirmation of promotion effect of adipocyte differentiation

Human bone marrow mesenchymal stem cells (hBM-MSCs) were purchased from Lonza, Inc. (Walkersbille, MD, USA) and cultured according to Lonza's guidelines. Adipocyte differentiation was carried out using a method recommended by Ron, except that troglitazone (TRO) was used instead of indomethacin to induce adipocyte differentiation of mesenchymal stem cells. Specifically, mesenchymal stem cells are differentiated into adipocytes by inserting 1 g / ml of insulin, 1 M of dexamethasone, DEXA, 0.5 mM of isobutylmethylxanthine (IBX) and 2 M of TRO into the culture medium of mesenchymal stem cells . In the case of the experimental group, 10 mM of the ginseng seed oil of Example 1, the ginseng seed residue extract of Example 2, troglitazone, and glibenclamide (purchased from Sigma-aldrich, USA) 10 days for two days), 10M each was added to the medium. After 10 days of induction of differentiation of mesenchymal stem cells into adipocytes, the cell culture medium was removed and the cells were washed with phosphate buffered saline (PBS). Cells were washed with 0.2 ml / cm ² of 10% formalin / PBS solution Was fixed for 10 minutes. The lipid bodies in a washed once fixed cells in 60% isopropanol solution 0.5ml / cm ^2, and oil red O (Oil Red O) for 10 minutes for dye dissolved in 60% isopropanol in 0.2ml / cm ² adipocytes Lt; / RTI > The dyed oil red oi was eluted with a 100% isopropanol solution, and the absorbance was measured at a wavelength of 500 nm. The results are shown in Table 5 below. Each data value was calibrated to an IDX negative control.

Treated group Oil Red Relative Dyeing Ratio Comparative Example 1.00 Example 1 (ginseng seed oil) 2.74 Example 2 (Ginseng seed residue extract) 1.75

As shown in the above table, the ginseng seed extract promoted the differentiation of adipocytes (P value <0.01).

Hereinafter, formulation examples of the composition according to the present invention will be described, but the present invention is not limited thereto.

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) Purified water Balance Ginseng seed extract 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient Content (% by weight) Purified water Balance Ginseng seed extract 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 3] Massage cream

Ingredient content (weight%) Purified water Balance Ginseng seed extract 0.1 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 4] Pack

Ingredient content (weight%) Purified water Balance Ginseng seed extract 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Pingyi-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount

1 is a graph showing the effect of inhibiting melanin production by ginseng seed extract according to the present invention.

Claims (33)

Wherein the ginseng extract is an effective ingredient and the ginseng is Panax ginseng CA Meyer. The composition for external application for skin according to claim 1, wherein the composition further comprises ginseng seed oil. delete delete The composition for external application for skin according to claim 1, wherein the ginseng seed residue extract is contained at a concentration of 0.001 to 10% by weight based on the total weight of the composition for external application for skin. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin has a use for protecting the skin by inhibiting skin damage. The composition for external application for skin according to claim 6, wherein the composition for external application for skin protects the skin through an action of inhibiting skin damage caused by oxidation or ultraviolet rays. 7. The composition for external application for skin according to claim 6, wherein the skin is scalp or hair. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin has a use for preventing or improving skin aging. 10. The composition for external application for skin according to claim 9, characterized by inhibiting aging of the skin through inhibition of skin damage due to oxidation or ultraviolet rays, promotion of collagen production or inhibition of biosynthesis of matrix metalloproteinase I (MMP-1) Wherein the external preparation is a skin composition. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin has a use for preventing or improving skin wrinkles. 12. The composition for external application for skin according to claim 11, wherein the composition for external application for skin is to prevent or improve wrinkles of the skin through promotion of collagen production or inhibition of biosynthesis of matrix metalloproteinase I (MMP-1). The composition for external application for skin according to any one of claims 1, 2 and 5, wherein the composition for external application for skin has application for improving skin elasticity. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin has a skin whitening application. 15. The composition for external application for skin according to claim 14, wherein the composition for external application for skin performs skin whitening through inhibition of melanin formation or whitening of pigment deposited on skin. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin is used for skin moisturizing. [17] The composition for external application for skin according to claim 16, wherein the composition for external application for skin maintains skin moisture through promotion of differentiation of keratinocytes or promotion of transglutaminase expression. The composition for external application for skin according to claim 16, wherein the composition for external application for skin has a use for preventing or ameliorating dry skin diseases including atopic or psoriasis. The composition for external application for skin according to any one of claims 1, 2 and 5, wherein the composition for external application for skin has a use for exfoliation of skin. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin has application for regulating skin sebum. 21. The composition for external application for skin according to claim 20, wherein the composition for external application for skin regulates the sebum of skin by suppressing secretion of sebum. The composition for external application for skin according to any one of claims 1, 2 and 5, wherein the composition for external application for skin has a use for preventing or ameliorating inflammation or relieving irritation. 23. The composition for external application for skin according to claim 22, wherein the skin is a scalp. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin has a use for preventing or improving skin troubles including acne. 25. The composition for external application for skin according to claim 24, wherein the skin is a scalp. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin has application for improving skin color. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the skin is scalp or hair. 28. The composition for external application for skin according to claim 27, wherein the composition for external application for skin has a use for prevention of hair loss. 28. The composition for external application for skin according to claim 27, wherein the composition for external application for skin has anti-dandruff application. 28. The composition for external application for skin according to claim 27, wherein the composition for external application for skin has application for improving gloss or softness of hair. The composition for external application for skin according to any one of claims 1, 2, and 5, wherein the composition for external application for skin has a volume or elasticity-enhancing use for a face or a body. 32. The composition for external application for skin according to claim 31, wherein the composition for external application for skin promotes the volume and elasticity of the face or body through promotion of adipocyte differentiation. The composition for external application for skin according to any one of claims 1, 2 and 5, wherein the composition for external application for skin has a use for improving the face or body line.

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