KR101707522B1 - Composition for applying to skin externally containing extract from flower of ginseng - Google Patents

Abstract

The present invention relates to the use of ginseng flower extracts, and the use of the ginseng flower extract is useful for skin protection, anti-aging, anti-wrinkle, skin elasticity improvement, prevention and improvement of skin dryness diseases such as skin whitening, , Prevention or improvement of skin troubles such as acne, exfoliation, improvement of skin color, prevention of hair loss, anti dandruff, improvement of gloss and softness of hair, volume or elasticity of face or body, improvement of face or body line.

Description

TECHNICAL FIELD The present invention relates to a composition for external application for skin containing ginseng flower extract,

The present invention relates to the use of ginseng flower extract. More particularly, the present invention relates to the use of ginseng flower extract for skin protection, anti-aging, anti-wrinkle, skin elasticity improvement, whitening, skin moisturizing, prevention and improvement of skin dryness diseases such as atopy, Anti-inflammatory use, prevention or improvement of skin troubles such as acne, exfoliation, improvement of skin color, prevention of hair loss, anti dandruff, improvement of gloss and softness of hair, volume or elasticity of face or body, improvement of face or body line Lt; / RTI >

Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogawa and Ginseng. It has been used for over 2,000 years in Korea, China and Japan. It has been empirically used to prevent disease and prolong its life. The known potency and effects of ginseng are as follows: action on the central nervous system, anti-cancer and anti-cancer activity, immune function regulation, anti-diabetic effect, liver function enhancement, cardiovascular disorder improvement and anti-arteriosclerosis effect, Antioxidant activity, antioxidant activity, and antioxidant activity of the present invention.

Ginsenoside, a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground of ginseng. However, ginsenoside content and composition vary greatly depending on the site such as ginseng roots, ginseng leaves, and ginseng fruit. In particular, ginseng blossom has been reported to have excellent hepatoprotective properties based on ginseng roots and other ginsenoside components and components (J. Nat. Prod, 66; 922-927, 2003.) .

Recently, consumers' interest in natural cosmetics has been increasing, and at the same time, many cosmetics utilizing herbal materials have been released, and ginseng has been studied as a plant material having important skin effect of cosmetics. However, most of them use ginseng roots. Specifically, only the extract of ginseng root or the ginsenoside component of ginseng root and ginseng polysaccharide are used, and there is no research on the skin effect by the ingredient of ginseng flower.

It is an object of the present invention to provide a skin external preparation useful for skin.

It is an object of one embodiment of the present invention to provide a composition for protecting the skin.

It is an object of another embodiment of the present invention to provide a composition for protecting hair.

It is an object of another embodiment of the present invention to provide a composition for preventing or improving aging.

It is an object of another embodiment of the present invention to provide a composition for preventing or improving wrinkles.

Another object of one embodiment of the present invention is to provide a composition for improving skin elasticity.

Another object of one embodiment of the present invention is to provide a composition for skin whitening.

Another object of the present invention is to provide a composition for skin moisturizing.

Another object of the present invention is to provide a composition for preventing or improving skin dryness diseases such as atopy.

 It is an object of another embodiment of the present invention to provide a composition for removing skin horny.

It is an object of another embodiment of the present invention to provide a composition for controlling skin sebum.

It is an object of another embodiment of the present invention to provide a composition for preventing or alleviating inflammation.

It is an object of another embodiment of the present invention to provide a composition for preventing or alleviating inflammation of the scalp.

Another object of the present invention is to provide a composition for preventing or improving skin troubles such as acne.

Another object of the present invention is to provide a composition for preventing or ameliorating skin troubles such as acne occurring on the scalp.

It is an object of another embodiment of the present invention to provide a composition for improving skin color.

Another object of the present invention is to provide a composition for preventing hair loss.

Another object of one embodiment of the present invention is to provide an anti-dandruff composition.

It is an object of another embodiment of the present invention to provide a composition for promoting gloss or softness of hair.

It is an object of another embodiment of the present invention to provide a composition for enhancing the volume and elasticity of a face or a body.

It is an object of another embodiment of the present invention to provide a composition for improving the face or body line.

To achieve the above object, the composition for external application for skin according to the present invention is characterized by containing ginseng flower extract as an active ingredient.

In one embodiment of the present invention, the composition for external application for skin is characterized by having a use for protecting the skin by inhibiting skin damage. Wherein the skin comprises scalp or hair. That is, in the present specification, a composition for external application for skin which protects the hair by suppressing damage to the scalp or hair is disclosed.

In another embodiment of the present invention, the composition for external application for skin is characterized in that the skin is protected through an action of suppressing skin damage caused by oxidation or ultraviolet rays. Wherein the skin comprises scalp or hair. That is, a composition for external application for skin which protects scalp or hair by inhibiting damage of scalp or hair by oxidation or ultraviolet rays is disclosed.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for suppressing skin aging.

In another embodiment of the present invention, the composition for external application for skin may be used for skin aging through inhibition of skin damage caused by oxidation or ultraviolet rays, promotion of collagen production, or inhibition of biosynthesis of matrix metalloproteinase I (MMP-1) Is suppressed.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for preventing or improving wrinkles of the skin.

In another embodiment of the present invention, the composition for external application for skin is characterized by preventing or improving wrinkles of the skin through promotion of collagen production or inhibition of biosynthesis of matrix metalloproteinase I (MMP-1).

In another embodiment of the present invention, the composition for external application for skin is characterized by having an application for improving skin elasticity.

In another embodiment of the present invention, the composition for external application for skin is characterized by having skin whitening application.

In another embodiment of the present invention, the composition for external application for skin is characterized by performing skin whitening through inhibition of melanin production or whitening of pigment deposited on the skin.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a skin moisturizing use.

In another embodiment of the present invention, the composition for external application for skin maintains skin moisture through promotion of differentiation of keratinocytes or promotion of transglutaminase.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for preventing or ameliorating skin dryness diseases including atopy or psoriasis.

In another embodiment of the present invention, the composition for external application for skin is characterized by having an application for exfoliation.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a skin sebum control purpose.

In another embodiment of the present invention, the composition for external application for skin is characterized in that sebum of the skin is controlled by inhibiting secretion of sebum.

In another embodiment of the present invention, the composition for external application for skin is characterized by having anti-inflammatory or anti-irritating properties. Here the skin contains the scalp. That is, in the present specification, there is provided a composition for external application for skin having the use of preventing or ameliorating inflammation of the scalp and alleviating irritation to the scalp.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for preventing or improving skin troubles including acne. Here the skin contains the scalp. That is, in the present specification, there is provided a composition for external application for skin having a use for preventing skin troubles including acne on the scalp from occurring and for improving skin troubles that have already occurred.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for improving skin color.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for preventing hair loss.

In another embodiment of the present invention, the composition for external application for skin is characterized by having anti-dandruff application.

In another embodiment of the present invention, the composition for external application for skin is characterized in that it has a use for improving gloss or softness of hair.

In another embodiment of the present invention, the composition for external application for skin is characterized in that it has a use for increasing the volume and elasticity of a face or a body.

In another embodiment of the present invention, the composition for external application for skin is characterized by having a use for improving lines of a face or a body.

In the present invention, ginseng root and ginseng flower extract having different composition and composition among the above-mentioned ginseng root components are contained as an active ingredient, and thus it is possible to provide skin protection, anti-aging, anti wrinkle, skin elasticity improvement, whitening, skin moisturizing, Prevention or improvement of skin, sebum control, anti-inflammation, prevention or improvement of skin troubles such as acne, exfoliation, improvement of skin color, prevention of hair loss, anti dandruff, improvement of gloss and softness of hair, improvement of volume and elasticity of face or body, It is possible to provide a composition for improving the line of the body.

1 is a graph showing the results of inhibition of active oxygen production of ginseng flower extract according to an embodiment of the present invention.
2 is a graph showing the activity of inhibiting DNA oxidative damage of ginseng flower extract according to an embodiment of the present invention.
FIG. 3 is a graph showing the effect of ginseng flower extract on the production of type I procollagen according to an embodiment of the present invention.
4 is a graph showing the effect of ginseng flower extract on the expression of MMP-1 according to an embodiment of the present invention.
5 is a graph showing the effect of ginseng flower extract on melanin production according to an embodiment of the present invention.

As used herein, the term "extract" refers to a substance obtained by extracting a component contained therein from a natural product, regardless of the method of extraction or the kind of the component. For example, it is a broad concept including both extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and extracts of specific components of natural products, such as oils, and extracts of specific components.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is a concept of the broadest notion including a scalp and hair as well as a tissue covering a body surface such as a face or a body.

In an embodiment of the present invention, the ginseng is Panax ginseng C. A. Meyer.

In an embodiment of the present invention, the ginseng flower extract may be mixed in an amount of 0.001 to 10 wt% based on the total weight of the composition, but is not limited thereto.

The ginseng flower extract according to an embodiment of the present invention can be prepared by dissolving an organic solvent (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.) or organic solvent containing water or water in dried ginseng flower at 1 to 10 times (wt / v%) and allowed to stand at room temperature for one day. Preferably, the above step is repeated twice to obtain an extract. Preferably, chlorophyll is removed from the extract, and the filtrate is concentrated in a vacuum concentrator to obtain a dry product of the ginseng flower extract.

The human skin undergoes changes due to various internal and external factors as it gets older. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction As the amount of ultraviolet rays reaching the surface of the sunlight increases and the environmental pollution is further intensified, free radicals and active noxious oxygen are increased, thereby decreasing the thickness of the skin, increasing the wrinkles and decreasing the elasticity However, skin coloration becomes dull and unpleasant, skin troubles frequently occur, and spots, freckles, and black blotch also increase.

As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid, and glycoprotein, which constitute the skin, change or decrease, and oxidative stress due to free radicals and active noxious oxygen occurs. Cox-2 (cyclooxygenase), an enzyme that produces a proinflammatory cytokine, known to cause inflammation in most cells constituting the skin by aging or ultraviolet rays, And the biosynthesis of Matrix metalloproteinase (MMP), an enzyme that degrades skin tissue, is increased by these inflammatory factors, and nitric oxide (NO) production by iNOS (inducible nitric oxide synthase) Respectively. In other words, decrease of cellular activity due to natural endogenous aging and decrease of biosynthesis of substrate material by microinflammation, increase of stress due to various harmful environment, increase of active oxygen species by sunlight, As the degradation and denaturation are accelerated, the skin substrate is destroyed and thinned, and various symptoms of skin aging appear.

The composition for external application for skin disclosed in the present specification contains a ginseng flower extract having an effect of inhibiting the production of active oxygen and inhibiting oxidative damage caused by active oxygen as an active ingredient so that damage to cells caused by active oxygen or ultraviolet rays It can prevent and protect the skin through this. Further, progress of aging by active oxygen or ultraviolet rays can be suppressed. In addition, the ginseng flower extract can prevent or ameliorate inflammation by inhibiting the biosynthesis of COX-2, thereby alleviating skin troubles. Here the skin contains the scalp. That is, the ginseng flower extract disclosed in the present specification can prevent damage to cells caused by active oxygen or ultraviolet rays generated from the scalp or hair, prevent or improve inflammation of the scalp, relieve the stimulation of the scalp by perming or dyeing It can be used for applications. The ginseng flower extract disclosed in the present specification can also be used for preventing or improving the wrinkles of the skin.

Several factors are involved in determining the skin color of a person. Among them, factors such as the activity of the melanocyte making the melanin pigment, the distribution of the blood vessel, the thickness of the skin, and the presence or absence of the pigment in and out of the human body such as carotenoid and bilirubin It is important.

Especially, the most important factor is melanin, a melanin pigment produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside of the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results.

The ginseng flower extract disclosed in the present specification can whiten skin by inhibiting the production of melanin. Thus, the composition for external application for skin disclosed in the present specification, which contains ginseng flower extract as an active ingredient, can be used for skin whitening purposes.

The most important function of the epidermis in the outermost skin is to protect against external stimuli (chemical, air pollutants, dry environment, physical and chemical stimulants such as ultraviolet rays) This protective function can maintain its function only when the stratum corneum composed of keratinocytes is normally formed. The outermost stratum corneum (horney layer) is formed from keratinocytes and consists of keratinocytes with complete differentiation and a surrounding lipid layer. The keratinocyte is a characteristic cell in which basal cells continuously proliferating in the lowest epidermis undergo a stepwise change in morphology and function and are elevated to the surface of the skin. After a certain period of time, And the new keratinocyte replaces its function. This repetitive sequence of changes is called "epidermal cell differentiation" or "keratinization". During keratinization, keratinocytes form the stratum corneum while producing natural moisturizing factors (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids), which make the stratum corneum firm and flexible, As shown in Fig.

However, such a stratum corneum can be easily lost due to excessive harshness, environmental factors such as living habits such as bath, dry atmosphere, pollutants, and endogenous diseases such as atopic skin and senile skin, In fact, due to the increasing number of factors in the modern age, there has been an increasing tendency in recent years to appeal to people with dry skin symptoms and disorders. Therefore, in order to maintain proper skin moisture, studies have been conducted to supply moisture from the outside or to prevent water loss from the body, and a variety of moisturizers having moisture retention ability have been developed, It is widely used in the area.

However, as the risk factors for humans increase in the living environment and the aging population rapidly increases, the turnover rate of the stratum corneum is delayed, the lipid synthesis ability of the keratinocytes decreases, The cells are not smoothly divided, maturated and differentiated, and the amount of moisturizing factors and lipids in the stratum corneum is decreased, so that the normal stratum corneum does not maintain its function. Namely, .

Due to the division and differentiation of abnormal abnormal epidermal cells, the skin causes various skin diseases such as dry skin, atopy and psoriasis. These diseases can alleviate the symptoms only by a conventional moisturizing agent having only moisture holding function , But it is difficult to expect fundamental healing.

The ginseng flower extract disclosed in the present specification promotes the differentiation of keratinocytes and thereby helps the process of elimination of old keratinocytes and the process of "differentiation of epidermal cells" or "keratinization", which is a generation process of new keratinocytes, Can effectively be removed. The composition for external application for skin disclosed herein may be useful as a composition for removing exfoliation by containing the ginseng flower extract having an excellent exfoliation effect as an effective ingredient.

In addition, the ginseng flower extract disclosed in this specification can keep the amount of moisturizing factor and lipid in the stratum corneum normally by keeping the turnover rate of the stratum corneum at a normal range. It also promotes the expression of transglutaminase. Through this, it is possible to moisturize the skin.

In addition, the ginseng flower extract disclosed in the present specification can promote the differentiation of keratinocytes and enhance the skin barrier function by maintaining skin moisture, thereby preventing or treating skin dryness diseases such as atopy and psoriasis.

In general, sebaceous glands in the skin, including the scalp and face, play a role in preventing moisturization and microbial invasion of the skin. However, when excess sebum secreted by sebum is secreted excessively, hair loss is promoted and acne is worsened. And seborrheic dermatitis is caused.

The hypersecretion of sebaceous glands is caused by various causes, and the most important of them is the activation of sebaceous glands by the amount of dihydrotestosterone (DHT), one of the hormones involved in promoting sebaceous gland secretion in sebaceous gland activity, It is overdone. That is, in the case of hair loss, testosterone (T) is converted into dihydrotestosterone, one of the male hormones, by the 5-alpha-reductase type 2 in cells, It binds to the receptor to cause hair loss. However, in skin and sebaceous glands, testosterone is converted to dihydrotestosterone by 5-α-reductase type 1 to activate sebaceous cells By accelerating the differentiation sebum in the sebaceous (sebum) is overdose to cause acne.

Acne and hair loss, which are skin troubles other than simple sebum hypersecretion, are further exacerbated by the minute inflammatory reaction of the skin. The cause of acne is that excess sebum accumulates in the hair follicle, activating acne activity and inducing inflammation.

The ginseng flower extract disclosed in the present specification can prevent or improve acne, enlargement of pores and seborrheic dermatitis due to excessive secretion of sebum by inhibiting sebum secretion.

The composition for external application for skin disclosed in the present specification can promote volume and elasticity of face and body by promoting differentiation of adipocytes. Applying the composition for external application for skin disclosed herein to areas where the volume and elasticity of the face and the body are required to be improved, volume and elasticity are enhanced. The area where volume and elasticity are required to be promoted may be a depressed area due to aging. For example, it may be a bow tortured by aging. Applying the composition for external application for skin disclosed herein to a depressed ball may promote volume by promoting adipocyte differentiation. Further, as another example in which the volume and the elasticity are required to be enhanced, a chest and a hip region are exemplified. When the composition for external application for skin disclosed in this specification is applied to the chest and the buttocks, the chest and buttocks are resilient and bulky.

In addition, the composition for external application for skin disclosed in this specification can improve the line of face and body. The ginseng flower extract as an active ingredient promotes the differentiation of adipocytes, thereby promoting volume and elasticity of the face and body, promoting collagen biosynthesis, inhibiting collagen degradation, Can be improved.

The cosmetic form according to the invention contains a cosmetically or dermatologically acceptable medium or base. It may be in any form suitable for topical application, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing the oil phase in water, a suspension, a microemulsion, a microcapsule, In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art. The composition according to the invention may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition of the present invention can be used in the form of a cosmetic or dermatological composition comprising a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, , Cosmetics such as fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants conventionally used in the field of dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

The external preparation for skin comprising the ginseng flower extract of the present invention is not particularly limited in its formulation, and examples thereof include softening longevity, astringent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, Cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil and body essence.

The composition for external application for skin according to the present invention may be a pharmaceutical composition. The pharmaceutical composition may additionally contain preservatives, stabilizers, wetting or emulsifying accelerators, pharmaceutical adjuvants such as salts and / or buffers for controlling osmotic pressure, and other therapeutically useful substances. The pharmaceutical composition may be formulated into lotions, creams, ointments or gels.

The pharmaceutical composition is preferably transdermally administered.

The dosage of the ginseng flower extract which is an effective ingredient of the pharmaceutical composition will depend on the age, sex, and weight of the subject to be treated, the particular disease or pathological condition to be treated, the severity of the disease or pathological condition, . Dosage determinations based on these factors are within the level of those skilled in the art. Generally, the dosage ranges from 0.0001 mg / kg / day to about 2000 g / kg / day.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to examples and test examples. However, these examples and examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Example 1] Production of ginseng flower extract

10 g of 70% (v / v) ethanol was added to 1 kg of dried ginseng flower, and the mixture was allowed to stand at room temperature for one day. The above process was repeated twice to obtain an extract, and the obtained extract was filtered, and the filtrate was concentrated to 2 L in a vacuum concentrator. Chlorophyll was then removed by removing the ether layer from the filtrate by adding an equal amount of ether. The above process was repeated three times, and the remaining extract was concentrated in a vacuum concentrator to obtain 150 g of dry solid.

[Example 2, Comparative Example 1]

The composition containing the ginseng flower extract prepared in Example 1 was mixed according to the composition shown in Table 1 below.

Component (% by weight) Example 2 Comparative Example 1 Ginseng flower extract (Example 1) 0.01 - Caprylic Capric Triglyceride 70 80 ethanol 10 10 water Balance Balance

[Test Example 1] Inhibition of active oxygen production

Antioxidant effect was investigated by comparing the ability to remove reactive oxygen species (ROS) from cells by ultraviolet (UV) irradiation. As a positive control, vitamin C (Sigma Co.), which is generally used to compare the antioxidant effect, was used. In addition, the test group was not used as a control group. (UVB 30 mJ / cm ² ) was applied to a human HaCaT keratinocyte monolayer culture system (P Boukamp, German Cancer Research Center, Heidelberg, Germany), and the extract prepared according to Example 1 10 ppm, 50 ppm, and 100 ppm, respectively. Then, DCFH-DA (2 ', 7'-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc.) was measured with a fluorescent plate reader. The control group not treated with ultraviolet light was set as 100, and the ratio of ROS generation was calculated as a ratio to 100. The results are shown in Table 2 and FIG.

Test substance ROS generation rate (%) Non-UVB 100 UVB 161 UVB + Example 1 (10 ppm) 78 UVB + Example 1 (50 ppm) 72 UVB + Example 1 (100 ppm) 70 UVB + vitamin C 100 uM 72

The ginseng flower extract of Example 1 according to the present invention showed an effect of significantly erasing active oxygen generated by UV as compared with the control group. This is an excellent activity similar to vitamin C used as an activity index of antioxidants. That is, the ginseng flower extract according to Example 1 of the present invention protects the skin including the scalp and hair by significantly eradicating the active oxygen which is a cause of skin damage, and furthermore suppresses the generation of wrinkles, It was confirmed that it could have a preventive effect.

[Experimental Example 2] Inhibitory activity against DNA oxidative damage by single cell gel electrophoresis (SCGE, Comet assay)

After the human fibroblasts were cultured for 24 hours, the ginseng flower extract of Example 1 and vitamin E were treated for each concentration. After 12 hours, the positive control group, H ₂ O ₂ (final concentration: 10-3M) The result of staining with the electrophoresis method was observed with a fluorescence microscope. At this time, the length (um) of the tail as a result of DNA cleavage in each of 25 cells was analyzed using an image analyzer, KOMET 3.1 (Kinetic Imaging, England) 2.

Test substance Tail length (탆) Inhibition rate (%) H ₂ O ₂ 112.4 - H ₂ O ₂ + Example 1 (10 ppm) 104.6 6.9 H ₂ O ₂ + Example 1 (50 ppm) 70.2 37.5 H ₂ O ₂ + Example 1 (100 ppm) 65.4 41.8 H ₂ O ₂ + vitamin E 10 ppm 68.9 38.7

In the results of Table 3, the ginseng flower extract of Example 1 by cell gel electrophoresis showed significant oxidative DNA damage inhibitory activity, and thus the ginseng flower extract according to the present invention inhibited the hydroxyl radical (.OH) , Which is a DNA oxidative damage inhibitor that suppresses DNA single strand break caused by DNA damage. This is an important protective effect for protecting the genes of cells of the skin including the scalp or hair.

[Experimental Example 3] Type I procollagen assay (type I procollagen assay)

Human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , and then replaced with a medium containing 1 ppm, 10 ppm, and 50 ppm of the ginseng flower extract of Example 1. On the third day of culture, the cells were harvested and the amount of type I procollagen produced by ELISA was quantitated. TGF-β (Human Transforming Growth Factor-β1, Roche Co.) was used as a positive control. The results were calculated by comparing the values obtained by comparing the values of the control group without the ginseng flower extract of Example 1 to 0 and the positive control group with 100. This is shown in Table 4 and FIG.

Type I Procollagen Biosynthesis (%) Negative control group 0 Example 1 (1 ppm) 7 Example 1 (10 ppm) 22 Example 1 (50 ppm) 31 TGF-β 10 ng / ml 100

In the normal human fibroblast monolayer culture system, the ginseng flower extract of Example 1 markedly promoted the formation of type I procollagen as compared with the control. That is, the ginseng flower extract of Example 1 promotes collagen production, thereby alleviating the collagen reduction phenomenon caused by aging of human skin.

[Experimental Example 4] MMP-1 expression inhibition assay

The human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ^{5, and} irradiated with ultraviolet ray B at 40 mJ / cm ² , and then replaced with a medium containing 1 ppm, 10 ppm and 50 ppm of the ginseng flower extract of Example 1. On the second day of culture, the cells were harvested and the amount of MMP-1 (matrix metalloproteinase I) produced by ELISA was quantitated. The results were compared with those of the control group not irradiated with ultraviolet light containing no test substance as 100. RA (Retinoic acid , Sigma Co.) was used as a positive control group.

MMP-1 biosynthesis (%) No-UV 100 UV 152 UV + Example 1 (1 ppm) 128 UV + Example 1 (10 ppm) 105 UV + ≪ / RTI > Example 1 (50 ppm) 92 UV + RA 10 uM 45

In the human normal fibroblast monolayer culture system, the ginseng flower extract of Example 1 according to the present invention significantly inhibited the expression of MMP-1 induced by UVB 40 mJ / cm ² . Therefore, the ginseng flower extract of Example 1 according to the present invention inhibits the biosynthesis of MMP-1, a skin tissue degrading enzyme caused by internal aging or external environmental factors, thereby suppressing collagen reduction in skin and improving wrinkles will be.

[Experimental Example 5] Effect of improving skin wrinkles on human skin

Forty subjects aged 30 to 50 years were examined for the nutritional cream containing the ginseng flower extract of Example 1 having the composition shown in the following Table 5 (Example 3) and those not containing the ginseng flower extract , A nutritional cream of the same composition as that of Example 3 (Comparative Example 2) was prepared, and the skin wrinkle-reducing effect was compared and evaluated (unit: wt%).

ingredient Example 3 Comparative Example 2 Ginseng flower extract 0.1 - Wax 10 10 Polysorbate 60 1.5 1.5 Piggy 60 hardened castor oil 2 2 Sorbitan sesquioleate 0.5 0.5 Liquid paraffin 10 10 Squalane 5 5 Caprylic / capric triglyceride
(Estasan: Uniqema (manufacturer)) 5 5 glycerin 5 5 Butylene glycol 3 3 Propylene glycol 3 3 Triethanolamine 0.2 0.2 Preservative, coloring, fragrance Suitable amount Suitable amount Purified water Balance Balance

Example 3 was used for the face side of the subject, and Comparative Example 2 was used for the right side for 3 months. The skin condition of both sides of the facial area before the use of the cream was measured, and then the same part was remeasured 3 months after using the cream to measure the change of the skin wrinkles. The skin wrinkles were measured with a Visiometer system (C + K company) by removing the wrinkles of the eye tail region with a replica in a constant temperature and humidity chamber at 24 ° C and a relative humidity of 40%. The amount of change in the skin wrinkles was calculated according to the following formula (1).

[Equation 1]

In the equation (1), "Tdi" is the measured site value at D90 and "Tdo" is the measured site value at D0.

As a result of calculation according to the above formula (1), skin wrinkles at the site using Comparative Example 2 showed a decrease of 6.1 ± 7% (mean ± standard deviation), while skin wrinkles at the site using Example 3 were 35 ± 8% Of the total wrinkles.

[Experimental Example 6] Effect of skin elasticity improvement in human body

The skin elasticity improving effect of Example 3 and Comparative Example 2 prepared in the composition of Table 6 was measured. Forty women aged 30 years or older were divided into two groups, and the nutritional creams of Example 3 and Comparative Example 2 were applied to the face for 2 weeks for 12 weeks in each group. The skin elasticity meter (Cutometer SEM 575, C + K Electronic Co., Germany) was used to measure skin elasticity. As a result of the calculation, the elasticity effect of the region using Comparative Example 2 was 0.21 ± 0.14 (mean ± standard deviation), while the elasticity effect of the region using Example 3 was 0.30 ± 0.10, Respectively. The resultant value indicates a property of skin viscoelasticity of the skin elasticity measuring device.

As shown in the above results, when the nutritional cream of Example 3 containing the ginseng flower extract of Example 1 was used, the skin elasticity was improved by about 140%.

[Experimental Example 7] Inhibitory effect of melanin on mouse pigment cells

In order to examine the inhibitory effect of the ginseng flower extract of Example 1 on the melanin formation, mouse pigment cells were used.

First, 10% fetal bovine serum, 100 nM (100 nM) of Dulbecco's modified Eagles media was injected into DMEM (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) 2-O-tetradecanoyphorbol-13-acetate and 1 nM cholera toxin at 37 ° C and 5% CO ₂ . The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, and the cells were cultured at a concentration of 10 ⁵ cells / well in a 24-well plate. After 10 days, 50 ppm, 100 ppm of ginseng flower extract (Example 1) was added and cultured. At this time, arbutin (Sigma Co.) was used as a positive control. Next, the culture solution was removed, washed with PBS, and the cells were dissolved with 1 N sodium hydroxide, and the absorbance at 400 nm was measured. The rate of inhibition of melanin production was calculated according to the following formula (2) (Dooley's method).

&Quot; (2) "

Melanin production inhibition rate (%) Control group (untreated group) 0 Example 1 (10 ppm) 4 Example 1 (50 ppm) 25 Example 1 (100 ppm) 49 Arbutin 100 ppm 52

As shown in Table 7, it was confirmed that the ginseng flower extract of Example 1 according to the present invention showed a similar inhibition rate of melanin formation to that of hydroquinone. As a result, it was confirmed that the ginseng flower extract of Example 1 according to the present invention showed excellent whitening effect.

[Experimental Example 8] Whitening effect on human skin

The following experiment was conducted to examine the whitening effect of the ginseng flower extract of Example 1 on human skin.

First, 12 healthy boys were exposed to a 1.5 cm diameter opaque tape at the upper part of the subject's body, and then they were exposed to ultraviolet rays (UVB) of about 1.5 to 2 times the minimum erythema dose of each subject. To induce blackening of the skin.

After the UV irradiation, the ginseng flower extract of Example 1 and hydroquinone were each applied, and one of the conditions was observed for 10 weeks without applying anything. The color of skin was measured with a colorimeter CR2002 (Minolta Co., Japan) every 1 week.

Then, the difference (ΔL *) between the start time of the application of the respective test substances and the completion time of the application of the test substances was calculated according to the following equation (3). On the other hand, the whitening effect is judged by comparison of DELTA L * between the sample application site and the control site. When the DELTA L * value is 2, the whitening of the deposited pigment is noticeable. .

&Quot; (3) "

DELTA L * = L * value at the time of completion of application - L * value at the start of application

Test substance The degree of skin color brightness (DELTA L *) Example 1 1.91 + 0.17 Hydroquinone (positive control) 1.89 ± 0.15 Vehicle (negative control) 0.51 + - 0.13

As shown in Table 8, it was confirmed that the ginseng flower extract of Example 1 according to the present invention showed a degree of skin color brightness similar to that of hydroquinone. This is because the ginseng flower extract improves pigmentation caused by ultraviolet light and brightens skin color.

[Experimental Example 9] Differentiation induction of human keratinocytes

The effect of the ginseng flower extract of Example 1 according to the present invention on the cell differentiation promoting effect was examined by measuring the amount of CE (Cornified Envelop) produced upon differentiation of keratinocyte and human skin cell line (HaCaT).

The primary cultured human keratinocytes were placed in a culture flask and adhered to the bottom. The test substances described in Table 9 below were added to the culture medium by concentration, and the cells were cultured for 5 days until the cells reached 70-80% Lt; / RTI > The cells were harvested and washed with PBS (phosphate buffered saline). Then, the cells were washed with 10 mM Tris-HCl buffer (Tris-HCl buffer containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) , pH 7.4), sonication, boiling, and centrifugation were suspended again in 1 ml of PBS, and the absorbance at 340 nm was measured. Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a criterion when evaluating the degree of cell differentiation. The test results are shown in Table 8 below, in which low calcium (0.03 mM) treated group and high calcium (1.2 mM) treated group are negative / positive control group, and low calcium concentration test substance is added.

Test substance The ability to differentiate in keratinocytes (%) Control group The low calcium treatment group (Ca ^{2 +} 0.03 mM) 100 The calcium treatment group (Ca ^{2 +} 1.2 mM) 215 Example 1 1 ppm 117 10 ppm 122 100 ppm 125

From the results of Table 9, it can be confirmed that the ginseng flower extract promotes differentiation in keratinocytes. This indicates that the elimination of old keratinocytes and normalization of turnover cycle, which is a generation cycle of new keratinocyte, can enhance skin moisturization. In addition, it was confirmed that ginseng flower extract can promote skin exfoliation.

[Experimental Example 10] Expression of transglutaminase in human keratinocytes

Human keratinocytes 96 welhyeong cells into a 5x10 ⁴ are in each well of the culture plate was attached for 24 hours. After the adhered skin cell line was treated with the test substance, after 2 days, the medium was removed and stored in a -20 ° C refrigerator. Cells treated with the substance were treated with acetone: ethanol (1: 1, v / v) stored at -20 ° C for 30 minutes at 4 ° C, followed by freezing-thawing twice Cells were fixed. After incubation at room temperature, the organic solvent was evaporated and treated with blocking (1% bovine serum albumin), transglutaminase antibody, HRP anti-mouse antibody, -phennyldiamine). Absorbance was measured at 490 nm and calibration was performed by measuring the background at 630 nm. The test results are shown in Table 10 below, in which low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were used as negative / positive control groups and low calcium concentration test substances were added.

Test substance Expression amount (%) of transglutaminase Control group The low calcium treatment group (Ca ^{2 +} 0.03 mM) 100 The calcium treatment group (Ca ^{2 +} 1.2 mM) 138 Example 1 1 ppm 98 10 ppm 119 100 ppm 141

From the results of Table 10, the ginseng flower extract of Example 1 according to the present invention showed better expression than the control group. Thus, it can be seen that the ginseng flower extract of Example 1 of the present invention improves the expression of transglutaminase.

[Experimental Example 11] Increase in skin moisturizing power in human body

50 men and women of 50-60 age group representing dry skin were divided into two groups, and Example 3 and Comparative Example 2 were applied twice daily to the face for 4 weeks in each group. (24 ° C, relative humidity: 40%) after one week, two weeks, and four weeks after application and before two weeks (six weeks after completion of application) Skin moisture was measured and the results are shown in Table 11 below. The test result is a percentage of the increment of the measured value after a certain period of treatment based on the coneometer value measured just before the start of the test.

Moisture growth rate (%) Test substance 1 week Two weeks 4 weeks 6 weeks Example 3 33 41 42 33 Comparative Example 2 31 34 34 15

Connio meter is a skin moisture analyzer that measures the amount of water present in the skin by measuring the electrical conductivity of the skin. As shown in Table 11, in the group to which the substance of Example 3 containing the ginseng flower extract of Example 1 was applied, the skin moisture amount was further increased as compared with the control group to which Comparative Example 2 was applied. Further, the values of the skin moisture measured 2 weeks after the stop of application of the nutritional cream of Example 3 (total of 6 weeks) were similar to those after 1 to 2 weeks, It was found that the skin moisture by the application of the ginseng flower extract according to the present invention was continuously maintained.

[Experimental Example 12] Effect of suppressing sebum secretion

In order to measure the oily skin mitigation effect, 20 women aged 20-45 years, 20 men with oily skin, 10 men and 10 women in the forehead region, and 10 women in the morning and evening, After 2, 3, and 4 weeks of application, the skin sebum secretion amount was measured using a skin moisture analyzer (Sebumeter SM810). The test was performed at 20 ° C and 20% relative humidity. The measurement results are shown in Table 12. The results were expressed as the average value of 10 subjects. In the case of oily skin, the measurement value is 220 or more, and in the case of normal skin, it is between 100 and 220.

Test substance Before application 1 week 2 weeks 3 weeks 4 weeks Comparative Example 2 246 240 239 235 230 Example 3 248 237 232 216 211

From the results of Table 12, it was confirmed that the nutritional cream of Example 3 containing the ginseng flower extract of Example 1 according to the present invention has an effect of suppressing excessive sebum secretion, and thus has sebum control effect. Therefore, it is possible to prevent acne and trouble caused by excessive sebum occurring in the skin including the scalp.

Experimental Example 13 Effect of LPS (Lipopolysaccharide) Induced Cyclooxygenase-2 (COX-2) on Biosynthesis

Human fibroblasts were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , and then subjected to LPS treatment. Then, the ginseng flower extract of Example 1 was replaced with a medium containing 1 ppm, 10 ppm and 100 ppm. On the second day of culture, cells were harvested and the amount of cyclooxygenase-2 (COX-2) produced using the Westren blot method was quantitated. The comparison with the value measured with a densitometer with the control group as 100 is shown in Table 13 below. The control group was an untreated group in which the ginseng flower extract of Example 1 was cultured without treatment.

Test substance (concentration: ppm) COX-2 biosynthesis (%) Example 1 100 56 10 64 One 89 Control group 100

As can be seen from the results of Table 13, the ginseng flower extract of Example 1 according to the present invention inhibits the inflammatory reaction by reducing the biosynthesis of cyclooxygenase-2 induced by LPS, which is an inflammatory reaction inducer, . In addition, this showed an efficacy to alleviate skin troubles.

[Experimental Example 14] Effect of improving acne and skin trouble

The subjects were 10 women with severe skin troubles including acne. The subjects were soaked in soap for 30 minutes in the temperature and humidity chamber, and the skin trouble was measured by comparing the number of acne and inflammation .

The subjects were allowed to use the nutritional creams of Example 3 and Comparative Example 2 for 4 weeks, respectively, and the degree of acne and skin troubles were compared. The results are shown in Table 14 below.

Example 1
(5 people) Comparative Example 1
(5 people) Skin Trouble Improvement Ratio 86% 32%

As shown in the results of Table 14, application of Example 3 containing the ginseng flower extract of Example 1 according to the present invention for 4 weeks resulted in reduction of the number of existing acnes and improvement of inflammatory accompanying trouble.

[Experimental Example 15] Improvement in color of human skin (face)

In order to evaluate the effect of accelerating the blood circulation in the skin of the composition containing the ginseng flower extract of the present invention, the degree of blood circulation in the skin was measured using LDPI (Laser Doppler Perfusion Imager). LDPI is a widely used and widely used device for measuring blood circulation in the skin. It is a highly sensitive device that can measure not only the velocity and quantity of blood in the capillaries of the skin but also the flow in the small arteries and the parenchyma .

The face was soaked with soap and allowed to adjust for 30 minutes. The initial value was measured using an IR (Infrad) camera with LDPI and a skin temperature measuring instrument. The initial blood flow in the lower part of the forehead was measured by LDPI and the initial temperature of the forehead, under the eyes and cheeks was measured using an IR camera.

Blood flow and skin temperature (占 폚) were compared with the initial measured values after using the Example 3 for a week, and the results are shown in Tables 15 and 16, respectively.

Face Before use After 1 week use medium 0.945 1.154

forehead Under the eyes cheek Before use (℃) 32.16 32.09 30.34 After 1 week use (℃) 33.67 33.81 33.75

As described above, as a result of using Example 3 containing the ginseng flower extract of the present invention, it was confirmed that the blood color was improved due to the peripheral blood circulation improving effect on the faces of the subjects, It is suggested that the flower extracts may contribute to effectively transmit nutrients of the skin and inhibit and retard skin aging.

[Experimental Example 16] Test for preventing hair loss

A study on the effect of preventing hair loss was conducted on 30 subjects who had an early hair loss phenomenon for 16 weeks. The compositions of Example 2 and Comparative Example 1 were used once a day for 16 weeks. After 16 weeks, the hair was collected and counted again. The results are shown in Table 17.

Treated group Number of dropouts (average) / standard deviation Comparative Example 1 82.80 / 10.32 Example 2 (Ginseng flower extract) 75.07 / 8.70

As shown in the above table, when the composition containing the ginseng flower extract was treated, it was confirmed that the number of dropouts decreased significantly compared with the comparative example.

[Experimental Example 17] Experiments for inhibiting dandruff

The above Example 1 (ginseng flower extract) was applied to P. ovale ATCC 12087) was tested using a broth dilution method. After double dilution, the cells were cultured at 32 ° C for 24 hours and 48 hours, and the presence or absence of bacterial growth was judged by the degree of suspension. The minimum concentration without bacterial growth was defined as Minimum Inhibition Concentration (MIC) Concentration). If the mixture is opaque and it is difficult to judge the growth of bacteria, it is confirmed by microscopic observation. The results are shown in the following table.

Treated group MIC (minimum inhibitory concentration) Example 1 (Ginseng flower extract) 0.05%

As can be seen from the above table, it can be seen that the degree of reduction of the MIC of the ginseng flower extract is excellent. Therefore, ginseng flower extracts can be effectively used to treat and prevent dandruff.

[Experimental Example 18] Hair gloss enhancement effect

0.1 g of the hair sample was washed with 0.1 g of shampoo, washed thoroughly with flowing water, and then the hair sample was dried at 25 ° C and 50% humidity for 6 hours. Then, 0.1 g of each of the compositions of Example 2 and Comparative Example 1 shown in Table 1 was applied, and gloss was measured using a gonios photometer in the following manner.

The hair samples were measured for gloss in a cone photometer equipped with a halogen lamp. The cone photometer has a light incident angle of 30 degrees and can obtain a reflected value from 0 to 90 degrees. The gloss was calculated by the following equation.

&Quot; (4) "

Glossiness (G) = Sf / d

Sf: the highest value among reflection values by specular reflection value

d: When the diffuse reflection value is 0 degree

The gloss is less diffuse and the more the specular reflection occurs, the better the result. The measurement results are shown in Table 19 below.

Treated group d Sf G Comparative Example 1 6.49 68.41 10.54 Example 2 (Ginseng flower extract) 3.58 83.20 23.24

From the results of the above table, it was confirmed that when the composition containing ginseng flower extract was treated, the hair gloss was significantly improved as compared with the comparative example.

[Test Example 19] Confirmation of promoting effect of adipocyte differentiation

Human bone marrow mesenchymal stem cells (hBM-MSCs) were purchased from Lonza, Inc. (Walkersbille, MD, USA) and cultured according to Lonza's guidelines. Adipocyte differentiation was carried out using a method recommended by Ron, except that troglitazone (TRO) was used instead of indomethacin to induce adipocyte differentiation of mesenchymal stem cells. Specifically, mesenchymal stem cells are differentiated into adipocytes by inserting 1 g / ml of insulin, 1 M of dexamethasone, DEXA, 0.5 mM of isobutylmethylxanthine (IBX) and 2 M of TRO into the culture medium of mesenchymal stem cells . In the experimental group, 10 mM of each of the ginseng flower extract, troglitazone, and glibenclamide (obtained from Sigma-aldrich, USA) of Example 1 was treated (at 10-day intervals for two days) 10M each was added to the medium. After 10 days of induction of differentiation of mesenchymal stem cells into adipocytes, the cell culture medium was removed and the cells were washed with phosphate buffered saline (PBS). Cells were washed with 0.2 ml / cm ² of 10% formalin / PBS solution Was fixed for 10 minutes. The lipid bodies in a washed once fixed cells in 60% isopropanol solution 0.5ml / cm ^2, and oil red O (Oil Red O) for 10 minutes for dye dissolved in 60% isopropanol in 0.2ml / cm ² adipocytes Lt; / RTI > The dyed oil red oi was eluted with a 100% isopropanol solution, and the absorbance was measured at a wavelength of 500 nm. The results are shown in the following table. Each data value was calibrated to an IDX negative control.

Treated group Oil Red Relative Dyeing Ratio Comparative Example 1.00 Example 1 (Ginseng flower extract) 2.18

As shown in the above table, it was confirmed that the ginseng flower extract promoted differentiation of adipocytes (P value <0.01).

Hereinafter, formulation examples of the composition according to the present invention will be described, but the present invention is not limited thereto.

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) Purified water Balance Ginseng flower extract (Example 1) 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient Content (% by weight) Purified water Balance Ginseng flower extract (Example 1) 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 3] Massage cream

Compounding ingredient Content (% by weight) Purified water Balance Ginseng flower extract (Example 1) 0.1 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 4] Pack

Compounding ingredient Content (% by weight) Purified water Balance Ginseng flower extract (Example 1) 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Pingyi-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount

Claims (8)

As an external composition for skin,
The composition contains 70% (v / v) ethanol extract of ginseng flower as an active ingredient,
The ginseng is Panax ginseng CA Meyer,
The extract is chlorophyll-free,
Wherein said composition has anti-dandruff usage by inhibiting the growth of the dysenteric pityrosporum ovalle ( Pityrosporum ovale ). The composition for external application for skin according to claim 1, wherein the ginseng flower extract is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. delete The composition for external application for skin according to claim 1, wherein the skin is scalp or hair. delete The composition for external application for skin according to claim 1, wherein the composition is a cosmetic composition. 7. The composition of claim 6, wherein the composition is selected from the group consisting of softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, , Body oil, and body essence. The composition for external application for skin according to claim 1, wherein said composition is a pharmaceutical composition.

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