CN111201011A - Skin external preparation composition containing ginseng condensate obtained by vacuum drying at normal temperature - Google Patents
Skin external preparation composition containing ginseng condensate obtained by vacuum drying at normal temperature Download PDFInfo
- Publication number
- CN111201011A CN111201011A CN201880066177.8A CN201880066177A CN111201011A CN 111201011 A CN111201011 A CN 111201011A CN 201880066177 A CN201880066177 A CN 201880066177A CN 111201011 A CN111201011 A CN 111201011A
- Authority
- CN
- China
- Prior art keywords
- ginseng
- cosmetic composition
- composition according
- condensate
- vacuum drying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a composition for external use containing ginseng condensate obtained by a normal temperature vacuum drying method, and more particularly, to a composition for external use containing ginseng condensate obtained by a normal temperature vacuum drying method, which has effects of preventing skin aging, anti-inflammation, or moisturizing by recovering effective ingredients of ginseng using ginseng condensate which is not an extract of ginseng or a steamed liquid of ginseng in such a manner that the effective ingredients of ginseng are not lost.
Description
Technical Field
The present application claims priority rights based on korean patent application No. 10-2017-0110958 filed on 31.2017 and korean patent application No. 10-2018-0099176 filed on 24.2018.08, and the entire contents disclosed in the corresponding korean patent application documents are included as part of the present specification.
The present invention relates to a composition for external use containing ginseng condensate obtained by a normal temperature vacuum drying method, and more particularly, to a composition for external use containing ginseng condensate obtained by a normal temperature vacuum drying method, which has effects of preventing skin aging, anti-inflammation, or moisturizing by recovering effective ingredients of ginseng using ginseng condensate which is not an extract of ginseng or a steamed liquid of ginseng in such a manner that the effective ingredients of ginseng are not lost.
Background
Ginseng (Panax ginseng c.a. meyer) is a plant belonging to the genus Panax of the family araliaceae, is a crude drug that has been used for more than 2000 years ago in korea, china, japan, and the like, and has been used empirically for the purpose of preventing diseases and prolonging life. The functions and effects of ginseng known so far are: effects on the central nervous system, anticancer effects, anticancer activities, immune function regulating effects, antidiabetic effects, liver function hyperactivity, cardiovascular disorder improvement, arteriosclerosis prevention effects, blood pressure regulation effects, climacteric disorder improvement, osteoporosis prevention effects, anti-stress and anti-fatigue effects, antioxidant activities, aging-inhibiting functions, and the like (the latest Korean ginseng "component and functional article", Korean ginseng tobacco institute, 56-112, 1996).
It is known that ginsenosides, which are representative physiologically active ingredients of ginseng, are uniformly distributed in the overground and underground parts of ginseng, and in particular, the composition of ginsenosides varies not only in terms of the content thereof but also in terms of the parts of ginseng roots (roots), ginseng leaves, ginseng fruits, etc. (Attele AS et al, Biochem Pharmacol, 58; 1685-1693, 1999).
Ginseng genus (Panax) korean ginseng (ginseng) has 4 varieties of purple stem variety (var. jakengsjong), yellow ripe variety (var. hwangsookjong), green stem variety (var. chungkeangjong), and orange ripe variety (var. deunghwangsookjong).
Accordingly, research into the preparation of skin cosmetics and pharmaceutical compositions using ginseng has been actively conducted.
For example, as a prior art regarding cosmetic compositions containing ginseng extracts, korean patent laid-open No. 10-1711002 describes a method for processing and improving ginseng leaves by a traditional Chinese medicine preparation method and a technique for cosmetic compositions containing an improved ginseng leaf extract as a main active ingredient, and korean patent laid-open No. 10-2013 0090029 discloses a skin external agent containing steamed ginseng seeds as a main ingredient and a method for preparing a skin external agent using the same, characterized in that the skin external agent consists of a steamed ginseng seed extract extracted by any one solvent selected from water, ethanol or n-hexane and steamed ginseng seed oil extracted from steamed ginseng seeds which have been subjected to a steaming treatment process.
However, the prior art as described above has the following limitations: the ginseng extract contains an extremely small amount of effective components of ginseng and thus fails to exhibit a significant effect; since the ginseng extract is prepared using an organic solvent, the organic solvent may remain; in order to extract a large amount of effective components, a plurality of steps need to be performed.
Therefore, there is a need to develop a cosmetic composition containing a large amount of ginseng active ingredients and exhibiting skin aging prevention, anti-inflammatory or moisturizing effects.
Documents of the prior art
Patent document
Korean granted patent No. 10-1711002 (preparation method of folium Ginseng extract and cosmetic composition containing the same as effective component)
Korean Korea laid-open patent No. 10-2013-0090029 (skin external preparation composition containing steamed ginseng seed as main ingredient and preparation method of skin external preparation containing steamed ginseng seed as main ingredient using the same)
Disclosure of Invention
In order to solve the above problems, the present inventors prepared cosmetic compositions containing ginseng condensate or ginseng steamed liquid obtained by a room-temperature vacuum drying method, and confirmed that these compositions are excellent in skin aging prevention, anti-inflammatory or moisturizing effects without containing substances harmful to the skin and further containing more effective ingredients of ginseng exhibiting skin aging prevention, anti-inflammatory or moisturizing effects than conventional ginseng extracts, thereby completing the present invention.
Accordingly, an object of the present invention is to provide a cosmetic composition containing ginseng condensate obtained by a vacuum drying method at room temperature, which has skin aging prevention, anti-inflammatory, or moisturizing effects by recovering only effective ingredients without losing effective ingredients of ginseng.
In order to achieve the above objects, according to one embodiment of the present invention, there is provided a cosmetic composition for preventing skin aging, comprising ginseng condensate obtained by a vacuum drying method at an ordinary temperature as an effective ingredient.
In addition, according to an embodiment of the present invention, a cosmetic composition for anti-inflammation, which contains ginseng condensate obtained by a vacuum drying method at normal temperature as an effective ingredient.
In addition, according to an embodiment of the present invention, a cosmetic composition for skin moisturizing, which contains ginseng condensate obtained by an ambient-temperature vacuum drying method as an effective ingredient.
The ginseng condensate can be a ginseng steamed liquid.
The cosmetic composition may contain 0.001 to 50 wt% of ginseng condensate with respect to the total weight of the composition.
The cosmetic composition may have a formulation selected from the group consisting of softening lotion, astringent lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, mask, powder, body cream, body oil, and body essence.
The cosmetic composition may be used for preventing skin aging by reducing the amount of MMP-1 (matrix metalloproteinase-1) expressed by ultraviolet irradiation.
The cosmetic composition may improve or alleviate skin inflammation by reducing the expression of inflammatory genes (cyclooxygenase-2, COX-2) increased by Interferon gamma (IFN-gamma).
The cosmetic composition may contribute to skin moisturization by increasing the production amount of hyaluronic acid concentration-dependently.
The cosmetic composition may be one which promotes collagen production.
The collagen may be procollagen reduced by UV irradiation.
The steamed liquid of panax japonicus may contain one or more compounds selected from Octamethylcyclotetrasiloxane (octamethylcyclotetramethoxyxane), decamethylcyclopentasiloxane (decamethylcyclopentasiloxane), and dodecamethylcyclohexasiloxane (dodecamethylcyclohexasiloxane).
According to an embodiment of the present invention, there is provided a method for preparing a cosmetic composition, the cosmetic composition being the cosmetic composition described above, the method comprising: s1) removing and cleaning foreign matter stuck on the ginseng; s2) preparing ginseng vapor by vacuum drying the cleaned ginseng at normal temperature; and S3) condensing the ginseng vapor to prepare ginseng condensate.
Optionally, a step of steaming the boiled ginseng with water vapor may be further included between the step of S1) and the step of S2).
The vacuum pressure in the step of S2) may be 5 to 150 kPa.
The cosmetic composition containing ginseng condensate obtained by a room-temperature vacuum drying method of the present invention comprises ginseng condensate from which only water is removed or ginseng steamed liquid, thereby preventing skin aging and inhibiting skin aging caused by ultraviolet rays.
In addition, the cosmetic composition of the present invention containing ginseng condensate or ginseng steamed liquid obtained by vacuum drying at room temperature can reduce the expression level of inflammatory genes to improve or alleviate skin inflammation.
In addition, the cosmetic composition of the present invention containing ginseng condensate or steamed ginseng solution obtained by vacuum drying at room temperature can contribute to moisture retention.
In addition, the cosmetic composition of the present invention containing the ginseng condensate or the steamed liquid of panax japonicus obtained by the vacuum drying method at normal temperature can mass-produce the cosmetic composition having skin aging prevention, anti-inflammatory or moisturizing effects through a simple preparation method.
Drawings
Fig. 1 is a diagram showing a room-temperature vacuum drying apparatus according to an embodiment of the present invention.
Fig. 2 is a graph showing the results of evaluating the skin aging prevention effect of example 1.
Fig. 3 is a graph showing the results of evaluating the anti-inflammatory effect of example 1.
Fig. 4 is a graph showing the results of evaluating the moisturizing effect of example 1.
Fig. 5 is a graph showing the results of evaluating cytotoxicity of example 2.
Fig. 6 is a graph showing the results of evaluating the collagen production effect of example 2.
(description of reference numerals)
100: drying apparatus
200: heating device
201: air channel
300: steam discharge pipe
400: steam condenser
401: compressor with a compressor housing
402: condenser
500: storage tank
Detailed Description
The cosmetic composition according to an embodiment of the present invention contains ginseng condensate obtained by a vacuum drying method at room temperature as an active ingredient.
The "ordinary temperature" mentioned in the present specification is an internal temperature when the pressure is reduced to vacuum, and means a temperature in the range of 20 ℃ to 37 ℃.
The "normal temperature vacuum drying method" mentioned in the present specification is a method of drying food or food, and is a drying method of drying food or food by rapidly transmitting pressure and energy in a vacuum state at normal temperature.
The room temperature vacuum drying method may be performed by a vacuum drying apparatus.
As an embodiment of the present invention, the vacuum drying apparatus is shown in fig. 1, and the vacuum drying apparatus may include: a dryer 100 which can accommodate ginseng; a heater 200 heating the inside of the dryer 100; a vapor discharge pipe 300 connected to the inside of the dryer 100; a vapor condenser 400 connected to a downstream side of the vapor discharge pipe 300; and a storage tank 500 connected to a downstream side of the vapor condenser 400 to store the ginseng condensate.
The dryer 100 may be a device capable of drying ginseng by depositing ginseng therein, and for this reason, the dryer 100 may be implemented in a container form in which the interior is sealed and a vacuum state can be maintained, and may be provided with a heater 200 at a lower portion thereof and a drying rack in which ginseng can be deposited and dried at an upper portion thereof.
The heater 200 directly or indirectly heats the dryer 100, and may be implemented to have an air passage 201 connected to the inside of the dryer 100, and the heated air passes along the air passage 201 while being indirectly thermally transferred to the inside of the dryer 100.
The vapor discharge pipe 300 is a passage through which air heated inside the dryer 100 is discharged to the outside, and a downstream side of the vapor discharge pipe 300 may be connected to the vapor condenser 400 such that vapor inside the dryer 100 is supplied to the vapor condenser 400 through the vapor discharge pipe 300.
The steam condenser 400 is a device for compressing and condensing steam supplied from the dryer 100 through the steam discharge pipe 300 to collect effective components of ginseng, and for this, the steam condenser 400 may include a compressor 401 and a condenser 402, in which case, the compressor 401 is a device connected to the steam discharge pipe 300 to compress steam, and the condenser 402 may be connected to a downstream side of the compressor 401 to condense the compressed steam.
The storage tank 500 is a device for storing the ginseng condensate collected by the steam condenser 400, and may be disposed at a downstream side of the steam condenser 400.
More specifically, a method for producing a cosmetic composition containing ginseng condensate as an active ingredient will be described later.
The "ginseng" mentioned in the present specification is a perennial herb belonging to the family Araliaceae and may include Panax japonicus, Panax ginseng, and Ginseng radix Rubri.
In addition, the "ginseng" in the present invention means ginseng that has just been harvested, and means ginseng that has not been air-dried or dried but has been harvested directly under natural conditions.
The white ginseng is harvested for 4-6 years to root, peeled and dried in the sun, dried to be straight and bent to be dried to be called as a curved ginseng. In addition, the red ginseng means ginseng obtained by steaming 5-6 year-old roots with skins with water vapor and drying the roots.
The "condensate" referred to in the present specification means a liquid produced by condensation of water vapor, for example, means water produced by phase change of water vapor by loss of heat. The condensate may typically have the same or similar degree of temperature as the water vapor.
The present invention will be described in detail below.
In one embodiment of the present invention, a cosmetic composition containing ginseng condensate obtained by a vacuum drying method at room temperature as an active ingredient can provide a cosmetic composition for preventing skin aging or improving wrinkles.
In addition, the cosmetic composition can regulate the balance of collagen lyase and collagen synthase by reducing the expression level of MMP-1 (matrix metalloproteinase-1) caused by ultraviolet irradiation, and prevent skin aging by reducing the expression level of MMP-1.
Further, an anti-inflammatory cosmetic composition containing ginseng condensate obtained by a vacuum drying method at room temperature as an active ingredient can be provided, which can improve skin allergy including acne by sebum regulation or anti-inflammatory effect, that is, by inhibiting the production of inflammatory factors.
In addition, the cosmetic composition can improve or alleviate skin inflammation by reducing the expression of cyclooxygenase-2 (COX-2) increased by Interferon gamma (IFN-gamma).
Further, a cosmetic composition for skin moisturizing containing ginseng condensate obtained by a vacuum drying method at room temperature as an active ingredient can be provided, and the cosmetic composition for skin moisturizing can contribute to skin moisturizing.
In addition, the cosmetic composition can increase the production amount of hyaluronic acid concentration-dependently to improve skin elasticity and skin wrinkles, while hyaluronic acid as a hydrophilic substance can provide excellent moisturizing effect to the skin.
In an embodiment of the present invention, the ginseng condensate may be a steamed ginseng solution. As described above, the ginseng includes ginseng, and the steamed liquid of ginseng means a liquid condensed by a normal temperature vacuum drying method after the steaming step.
The "ginseng condensate" refers to a liquid obtained by a vacuum drying method at room temperature, and means a liquid obtained by vacuum drying an undried or dried ginseng at room temperature to condense water vapor contained in the ginseng.
The water-ginseng steamed liquid may include one or more compounds selected from Octamethylcyclotetrasiloxane (octamethylcyclotetramethoxyxane), decamethylcyclopentasiloxane (decamethylcyclopentasiloxane), and dodecamethylcyclohexasiloxane (dodecamethylcyclohexasiloxane). Thus, the cosmetic composition can further improve the skin aging prevention, anti-inflammatory or moisturizing effect by containing the steamed ginseng liquid, compared to the case of containing the ginseng condensate.
In one embodiment of the present invention, the cosmetic composition promotes collagen production, increases the amount of collagen production in a concentration-dependent manner, cuts skin dryness to improve moisturizing power, improves skin elasticity, and improves skin wrinkles.
In this case, the collagen may be procollagen (procollagen) decreased by UV irradiation. Among them, procollagen is a protein component constituting the dermis layer of the skin, and plays a role in maintaining the moist feeling and elasticity of the skin by combining with moisture. In addition, since procollagen is synthesized in cells and then secreted out of cells to polymerize into collagen fibers, the production of procollagen can be increased to promote collagen synthesis.
In one embodiment of the present invention, the cosmetic composition may contain 0.001 to 50% by weight of ginseng condensate with respect to the total weight of the composition, and when the ginseng condensate is less than 0.001% by weight with respect to the total weight of the cosmetic composition, there may be no skin aging prevention or anti-inflammatory effect, and when it is more than 50% by weight, there may be caused an allergic reaction to the skin to cause an unexpected side effect. Preferably 0.01 to 30 wt%, more preferably 0.05 to 25 wt%, but not limited thereto.
In one embodiment of the present invention, the cosmetic composition may further comprise a cosmetically or dermatologically acceptable medium or base commonly used in the art. They can be provided in all forms suitable for topical application by methods conventional in the art, such as solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranule spheres or ionic (liposome) and/or non-ionic vesicle dispersion forms, or in cream, emollient water, emulsion, powder, ointment, spray or concealer stick forms. These compositions may be prepared by methods conventional in the art.
In an embodiment of the present invention, the cosmetic composition may have a formulation selected from the group consisting of softening lotion, astringent lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, mask, powder, body cream, body oil, and body essence.
In addition, regarding the cosmetic composition, those skilled in the art can select and formulate other components than the above-mentioned essential components in each dosage form as appropriate depending on the kind of other external preparations or the purpose of use, etc. without any difficulty. For example, ultraviolet ray blocking agents, hair conditioners, perfumes, and the like may be further contained.
When the dosage form of the present invention is a solution or emulsion, a solvent, solubilizer or opacifying agent, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan is used as a carrier component.
When the dosage form of the present invention is a suspension, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or traganth (r) gum may be used as a carrier component.
When the formulation of the present invention is a paste, a paste or a gel, animal oil, plant oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide can be used as a carrier component.
When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component, and particularly when a spray, a propellant such as chlorofluorocarbon, propane/butane or dimethyl ether may be additionally contained.
In one embodiment of the present invention, the cosmetic composition may additionally comprise a thickener. The thickener contained in the cosmetic composition may be methylcellulose, carboxymethylcellulose, carboxymethyl hydroxy guanine, hydroxymethyl cellulose, hydroxyethyl cellulose, carboxyvinyl polymer, polyquaternary ammonium salt, cetearyl alcohol, stearic acid, carrageenan, or the like, preferably one or more of carboxymethylcellulose, carboxyvinyl polymer, and polyquaternary ammonium salt may be used, and most preferably carboxyvinyl polymer is used.
In one embodiment of the present invention, the cosmetic composition may contain various bases and additives as appropriate, and the inventors can easily select the kinds and amounts of these components. Acceptable additives may be contained as necessary, and for example, preservatives, pigments, additives and the like which are commonly used in the art may be additionally contained.
The antiseptic can be Phenoxyethanol (phenoxythane) or 1, 2-Hexanediol (1, 2-hexane diol) and the like, and the perfume can be synthetic perfume and the like.
In one embodiment of the present invention, the cosmetic composition may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular peptides, high molecular polysaccharides, sphingolipids, and seaweed extracts. Examples of the other formulation ingredients that may be added include oil and fat ingredients, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, ethanol, pigments, perfumes, blood circulation promoters, cooling agents, antiperspirants, purified water, and the like.
The other formulation components that can be added are not limited to these, and any of the above components may be added within a range that does not impair the object and effect of the present invention.
The method of preparing the cosmetic composition according to an embodiment of the present invention includes: s1) removing and cleaning foreign matter stuck on the ginseng; s2) preparing ginseng vapor by vacuum drying the cleaned ginseng at normal temperature; and S3) condensing the ginseng vapor to prepare ginseng condensate.
The S1) step is a step of removing and cleaning foreign matters stuck on the ginseng, and is a preparation step of removing soil or pesticides stuck on or sticking to the ginseng for vacuum drying.
In this case, the foreign matter may include soil, agricultural chemicals, stones.
The cleaning is preferably performed with running water to prevent ginseng damage, but is not limited as long as it is a ginseng cleaning method commonly used in the art. In addition, because of cleaning with water, ginseng can be referred to as water ginseng.
S2) is a step of vacuum-drying the cleaned ginseng at normal temperature to prepare ginseng vapor, and the step S2) is a step of drying the ginseng by evaporating the water contained in the ginseng at normal temperature, using the principle that the water is easily evaporated at normal temperature by lowering the pressure and maintaining the ginseng in a vacuum state to lower the boiling point of water.
In this case, the vacuum drying apparatus described above can be used. Specifically, the pressure may be reduced to a vacuum state after the cleaned ginseng is accumulated in the dryer 100. In addition, the temperature inside the dryer 100 may be maintained at a normal temperature by the heater 200 connected to the dryer 100.
The normal temperature vacuum drying is a method of drying ginseng by rapidly transferring pressure and energy in a vacuum state at normal temperature.
The step of S2) is a step of extracting active ingredients contained in ginseng, which exhibit skin aging prevention or anti-inflammatory effects, with steam.
In the S2), the vacuum pressure may be 5 to 150kPa, wherein the vacuum pressure means the pressure inside the dryer 100 or the space to be dried. At this time, when the vacuum pressure is less than 5kPa, the pressure is too low, and thus, components other than the effective components of ginseng may be vaporized together, and when the vacuum pressure is more than 150kPa, the steam generation rate of ginseng may be reduced. Preferably 10kPa to 99kPa, more preferably 80kPa to 100kPa, but not limited thereto.
In the step S2), the heating temperature is preferably set to a normal temperature of 20 to 37 ℃, and the drying time is preferably 2 to 15 hours. When the drying time is less than 2 hours, the effective components of ginseng may not be completely vaporized, and when the drying time is more than 15 hours, other foreign substances that are not the effective components of ginseng may be vaporized together. Preferably, it is 5 hours to 10 hours, but is not limited thereto.
In an embodiment of the present invention, a step of steaming the ginseng using water vapor may be further included between the step of S1) and the step of S2).
The steaming is to steam ginseng with heated steam, and can induce changes in the active ingredients of ginseng by steaming, and is expected to prevent skin aging or improve the anti-inflammatory effect.
The steaming time may be 30 minutes to 2 hours, and when the steaming time is less than 30 minutes, it is difficult to induce a change in the effective components of ginseng, and when the steaming time is more than 2 hours, the effective components of ginseng may be scattered and evaporated together with water vapor. It may be preferable to be 30 minutes to 1 time, but is not limited thereto.
The steaming temperature is 80 to 120 ℃, preferably 90 to 110 ℃, but is not particularly limited as long as it is a temperature at which ginseng can be steamed.
The S3) step is a step of condensing the ginseng vapor to prepare a ginseng condensate, and is a step of condensing and liquefying the vapor containing the ginseng active ingredient.
At this time, the ginseng condensate may be prepared using the steam condenser 400 of the vacuum drying apparatus described above. Specifically, the ginseng vapor supplied through the vapor discharge pipe 300 is delivered to the vapor compressor 401. The ginseng vapor may then be compressed via the compressor 401 of the vapor compressor 401, and the compressed ginseng vapor is condensed by the condenser 402 of the vapor compressor 401. The condensed ginseng vapor may then be collected into a storage tank 500 located downstream of the condenser 402.
Hereinafter, preferred examples are provided to aid understanding of the present invention. However, the following embodiments are only for illustrating the present invention, and it is apparent to those skilled in the art that various changes and modifications may be made within the scope and technical spirit of the present invention, and these changes and modifications also fall within the scope of the appended claims.
Preparation example 1: preparation of ginseng condensate
1) Cleaning: after ginseng was obtained through Korean Korea ginseng wholesale center, soil attached thereto was removed.
2) And (3) normal-temperature vacuum drying: putting the ginseng into a vacuum dryer 100, setting the vacuum pressure to be 90-100 kPa and the heating temperature to be 20-37 ℃, and then drying for 5-10 hours.
3) Preparing a condensate: condensing the ginseng vapor obtained by vacuum drying at normal temperature and preparing a ginseng condensate.
Preparation example 2: preparation of steamed ginseng liquid
1) Cleaning: after obtaining ginseng through Korean Korea Korean ginseng wholesale center, soil attached thereto was removed.
2) Steaming: steaming Ginseng radix with water vapor at 100 deg.C for 30 min.
3) And (3) normal-temperature vacuum drying: putting the steamed ginseng into a vacuum drier 100, setting the vacuum pressure to be 90-100 kPa and the heating temperature to be 20-37 ℃, and then drying for 5-10 hours.
4) Preparation of a steamed liquid: condensing the ginseng vapor obtained by vacuum drying at normal temperature and preparing the ginseng steamed liquid.
Example 1
A composition comprising the ginseng condensate prepared in the preparation example 1.
Example 2
A composition containing the boiled liquid of panax japonicus prepared in the preparation example 2.
Experimental example 1: analysis of composition by GC-MS
In order to confirm the components of the ginseng condensate prepared from the preparation example 1 and to perform component comparison with the boiled water ginseng liquid prepared from the preparation example 2, Headspace gas chromatography mass spectrometry (Headspace GC-MS) was performed. 2g of the ginseng condensate and the steamed liquid of the panax japonicus were taken and put into a Headspace bottle (Headspace visual), heated at 80 ℃ for 30 minutes, and then injected into GC-MS to perform composition analysis. At this time, the operation conditions of GG-MS are as follows. The injection port temperature was 250 ℃ and an HP-5 column (column) was used as a GC column. After the oven temperature was held at 40 ℃ for 2 minutes, it was ramped up at 5 ℃/minute and held at 300 ℃ for 5 minutes. As a Carrier gas (Carrier gas), helium (He) was used at a rate of 1 mL/min. The m/z region was analyzed (20-550) using a scanning mode (SCAN mode) and the detected peak mass spectra were qualitatively confirmed by library (library) search. The results are shown in table 1 below.
[ Table 1]
As shown in Table 1 above, the steamed liquid of Panax japonicus contains octamethylcyclotetrasiloxane (octamethylcyclotetrasiloxane), Decamethylcyclopentasiloxane (Decamethylcyclopentasiloxane) and dodecamethylcyclohexasiloxane (dodecamethylcyclohexasiloxane), unlike the ginseng condensate.
On the other hand, unlike the boiled liquid of panax japonicus, the ginseng condensate contains 2-ethyl-1-hexanol (2-ethyl-1-hexanol) and 2-Isopropyl-3-methoxypyrazine (2-isoproxyl-3-methoxypyrazine).
Thus, it was confirmed that the effective components contained differ depending on the presence or absence of the steaming process.
Experimental example 2: skin aging preventing effect of ginseng condensate
In order to confirm the skin aging prevention effect of ginseng condensate, it was confirmed whether the expression level of MMP-1 increased by ultraviolet rays was reduced by treating ginseng condensate, and the results are shown in fig. 2.
First, human fibroblasts were treated at 7.5 × 104The concentration of each was inoculated (seeding) in a 12-well plate incubator by using DMEM medium containing 10% FBS (fetal bovine serum). The next day, the medium was cleaned with PBS and then put in DMEM medium without FBS for at least 6 hours of cell starvation culture (cellstarvation). Then, the medium was cleaned with PBS and then washed at 20mJ/cm2After UVB irradiation, DMEM without FBS according to the example 1 was added at various concentrations (50ppm, 100ppm, 200ppm) and cultured for additional 48 hours. The next day of culture medium was harvested (harvest) and the amount of MMP-1 (matrix metalloproteinase 1, matrixmetalloprotenase-1) produced was quantified by ELISA. As a result, a comparative value was calculated by comparing the measured value with a control group containing no test substance as 100, and 2. mu.M of RA (Retinoic acid); Sigma was used as a positive control group. In addition, the ultraviolet control group means that the control group containing no test substance was irradiated with UVB.
As shown in FIG. 2, it was confirmed that the ginseng condensate of example 1 was able to reduce the expression level of MMP-1 increased by ultraviolet irradiation by 90% to 100%. In addition, it was confirmed that the MMP-1 expression level was significantly reduced even when a very small amount of 50ppm of ginseng condensate was treated.
Among them, MMP-1 is an enzyme which acts to decompose collagen, and when the amount of MMP-1 is increased, the skin firmness is decreased, and wrinkles are easily formed. Therefore, it was confirmed that the ginseng condensate of the present invention has an effect of improving or preventing wrinkles.
Experimental example 3: anti-inflammatory effect of ginseng condensate
In order to confirm the anti-inflammatory effect of ginseng condensate, it was confirmed whether or not the treatment of ginseng condensate reduced the expression level of inflammatory genes increased by Interferon gamma (IFN- γ).
5 x 10 in 6-well plate culture device5Human keratinocytes were cultured at the respective concentrations, and 24 hours later, the culture was replaced with DMEM medium containing 125ppm, 250ppm and 500ppm of the ginseng condensate of example 1 and subjected to pretreatment. Interferon gamma (50ng/ml) was treated 6 hours after pretreatment to initiate inflammation. After 24 hours (harvest) cells were harvested and analyzed by RT-qPCR for the amount of cyclooxygenase-2 (COX-2: cyclooxgene-2) produced. The gene expression level was calculated based on an Internal standard (Internal control) RPLP0 and the comparative values are shown in fig. 3. In this case, the control group was the non-treated group in which the ginseng condensate of example 1 was cultured without treatment. In addition, the IFN- γ control group is a group to which interferon γ is treated.
As shown in fig. 3, it was confirmed that the ginseng condensate of example 1 decreased the expression amount of cyclooxygenase-2 increased by interferon γ by about 40% to 60%.
Among them, cyclooxygenase-2 is an enzyme related to inflammation, and a mechanism for treating inflammation by selectively inhibiting cyclooxygenase-2 is known. Therefore, it was confirmed that the ginseng condensate of the present invention inhibits cyclooxygenase-2, thereby confirming its therapeutic and prophylactic effects on inflammation.
Experimental example 4: moisturizing effect of ginseng condensate
In order to confirm the moisturizing effect of the ginseng condensate, the amount of Hyaluronic Acid (HA) produced by treating the ginseng condensate with HaCaT cells, which are keratinocyte cell lines, was measured and is shown in fig. 4.
First, DMEM medium containing 100 units/ml (units/ml) of penicillin-streptomycin (penicillin-streptomycin) and 10% FBS was used at 37 ℃ and 5% CO2And culturing a keratinocyte strain, namely HaCaT cells in a thermostat, subculturing once every 3-4 days, and then evaluating the moisture retention.
The HaCaT cells cultured as above were cultured at 5 × 105Seed (seed) into a 24-well plate incubator, and replace the medium with serum-free after 18 hoursserum-free) DMEM, the condensate of example 1 was treated in different wells at various concentrations (1%, 10%, 20%). After 24 hours, the medium was collected, centrifuged at 15000Xg for 5 minutes, and the supernatant was collected and the amount of hyaluronic acid produced was quantified by ELISA. At this time, the medium was collected and stored at-20 ℃ until ELISA analysis. The ELISA used an HA-ELISA kit (Elapscience Biotechnology Co., Ltd., Elapscience, Ltd.). As a result, a comparative value was calculated by comparing the measured value with a control group containing no test substance as 100, and 2. mu.M of RA (Retinoic acid); Sigma was used as a positive control group.
As shown in fig. 4, it was confirmed that the amount of hyaluronic acid produced, which is a factor related to moisture retention, was increased in a concentration-dependent manner as a result of treating the induced-to-dryness keratinocytes (human kerationcells) in example 1 at different concentrations. Hyaluronic acid is one of skin components, and if the amount of hyaluronic acid in the skin is reduced, skin aging is induced, and skin wrinkles are induced due to the reduction of elasticity. In addition, hyaluronic acid has many hydroxyl groups (-OH), and thus it has a moisturizing effect on the skin of animals as a hydrophilic substance. Therefore, it was confirmed that the ginseng condensate of the present invention induces the production of hyaluronic acid, and has not only an anti-wrinkle effect but also an excellent moisturizing effect.
Experimental example 5: evaluation of skin aging prevention Effect of steamed liquid of Panax japonicus-cytotoxicity
In order to confirm the skin aging prevention effect of the steamed ginseng, the steamed ginseng was treated at different concentrations according to example 2 to confirm whether the toxicity of cells after Ultraviolet (UVB) treatment was blocked, and the results are shown in fig. 5.
First, Human Dermal Fibroblasts (HDFn) were regenerated at 7.5 × 104The concentration of each was inoculated (seeding) in a 24-well plate incubator by using DMEM medium containing 10% FBS (fetal bovine serum). The next day, the medium was cleaned with PBS and then put into DMEM medium without FBS for at least 6 hours of cell starvation culture (cell culture). Then, the medium was cleaned with PBS and then washed at 20mJ/cm2The irradiation of the UVB is carried out,then, DMEM without FBS containing the steamed liquid of the aforementioned Panax ginseng of example 2 at various concentrations (0.5 wt%, 1 wt%, 2 wt%) was added and cultured for 48 hours, the cell culture medium was harvested the next day of culture (harvest) and cytotoxicity was measured using MTT kit, and as a result, a control group containing no test substance was used as 100 and compared with the measured value to calculate a comparative value, and TGF β at 5ng/ml was used as a positive control group.
As shown in fig. 5, it was confirmed that the steamed liquid of example 2 did not affect cytotoxicity under the ultraviolet irradiation condition. In addition, it was confirmed that the cell survival rate was 98% even when a very small amount of 0.5 wt% steamed ginseng solution was treated.
Therefore, it was confirmed that the steamed liquid of panax japonicus according to the present invention can block cytotoxicity and maintain cell viability even under ultraviolet irradiation, thereby preventing skin aging caused by ultraviolet rays.
Experimental example 6: evaluation of collagen production as skin aging prevention Effect of steamed liquid of Panax japonicus
In order to confirm the skin aging prevention effect of the steamed liquid of panax japonicus, the steamed liquid of panax japonicus of example 2 was treated at different concentrations to confirm whether the collagen production was promoted in the cells after the Ultraviolet (UVB) treatment, and the results are shown in fig. 6.
First, Human Dermal Fibroblasts (HDFn) were regenerated at 7.5 × 104The concentration of each was inoculated (seeding) in a 24-well plate incubator by using DMEM medium containing 10% FBS (fetal bovine serum). The next day, the medium was cleaned with PBS and then put into DMEM medium without FBS for at least 6 hours of cell starvation culture (cell culture). Then, the medium was cleaned with PBS and then washed at 20mJ/cm2After irradiation with UVB, DMEM without FBS containing the steamed ginseng solution of example 2 at various concentrations (0.5 wt%, 1 wt%, 2 wt%) was added and cultured for additional 48 hours. Cell culture media were harvested the next day of culture (harvest) and used the procollagen type IC-peptide EIA kit (Takara Bio), Cat ID (Cat Biotechnology), Cat IDNo.) MK101, japan), the amount of procollagen secreted into the culture medium was measured, the procollagen production amount was calculated by diluting the standard solution contained in the collagen measurement kit to various concentrations and measuring absorbance at 450nm to prepare a standard concentration curve, and as a result, a comparative value was calculated by comparing the measured value with a control group containing No test substance as 100, and a positive control group used TGF β of 5ng/ml, and a control group means a case where UVB was irradiated without a test substance.
As shown in fig. 6, it was confirmed that the amount of procollagen produced, which is a factor involved in wrinkle improvement and moisture retention, increased in concentration-dependently, as a result of treating the human dermal fibroblasts irradiated with ultraviolet rays in example 2 at different concentrations. Procollagen is produced intracellularly, and is a substance that polymerizes into collagen when released outside the cell. In addition, collagen in the skin plays a role in preventing the dispersion of moisture in the skin caused by the external environment, and is related to skin moisture retention, and locks moisture in the skin to prevent skin wrinkles and aging. Therefore, it was confirmed that the steamed ginseng liquid of the present invention promotes collagen production, and has not only an effect of preventing wrinkles but also an excellent moisturizing effect.
Claims (15)
1. A cosmetic composition for preventing skin aging comprises ginseng condensate obtained by vacuum drying at room temperature as an effective ingredient.
2. A cosmetic composition for anti-inflammatory contains ginseng condensate obtained by vacuum drying at room temperature as an effective ingredient.
3. A cosmetic composition for moisturizing skin contains ginseng condensate obtained by vacuum drying at room temperature as an effective ingredient.
4. The cosmetic composition according to any one of claims 1 to 3,
the ginseng condensate is a boiled ginseng liquid.
5. The cosmetic composition according to claim 1,
the cosmetic composition contains 0.001 to 50 wt% of ginseng condensate with respect to the total weight of the composition.
6. The cosmetic composition according to any one of claims 1 to 3,
the cosmetic composition has a formulation selected from the group consisting of softening lotion, astringent lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, mask, powder, body lotion, body cream, body oil, and body essence.
7. The cosmetic composition according to claim 1,
the cosmetic composition can prevent skin aging by reducing MMP-1 expression caused by ultraviolet irradiation.
8. The cosmetic composition according to claim 2,
the cosmetic composition improves or alleviates skin inflammation by reducing the expression amount of an inflammatory gene increased by interferon gamma.
9. The cosmetic composition according to claim 3,
the cosmetic composition contributes to skin moisturization by increasing the production amount of hyaluronic acid concentration-dependently.
10. The cosmetic composition according to claim 4,
the cosmetic composition promotes collagen production.
11. The cosmetic composition according to claim 10,
the collagen is procollagen reduced by UV irradiation.
12. The cosmetic composition according to claim 4,
the steamed liquid of rhizoma anemarrhenae contains one or more compounds selected from octamethylcyclotetrasiloxane, decamethylcyclopentasiloxane and dodecamethylcyclohexasiloxane.
13. A method for producing a cosmetic composition according to any one of claims 1 to 3,
the preparation method of the cosmetic composition comprises the following steps:
s1) removing and cleaning foreign matter stuck on the ginseng;
s2) preparing ginseng vapor by vacuum drying the cleaned ginseng at normal temperature; and
s3) condensing the ginseng vapor to prepare a ginseng condensate.
14. The method for producing a cosmetic composition according to claim 13,
a step of steaming the boiled ginseng using water vapor between the step of S1) and the step of S2).
15. The method for producing a cosmetic composition according to claim 13,
and the vacuum pressure in the step S2) is 5-150 kPa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310063217.6A CN115844788A (en) | 2017-08-31 | 2018-08-30 | Skin external preparation composition containing steamed Stichopus japonicus liquid obtained by vacuum drying at room temperature |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2017-0110958 | 2017-08-31 | ||
| KR1020170110958A KR102440460B1 (en) | 2017-08-31 | 2017-08-31 | Composition for external application for skin containing ginseng condensed water obtained by vacuum drying at room temperature |
| KR10-2018-0099176 | 2018-08-24 | ||
| KR1020180099176A KR102540470B1 (en) | 2018-08-24 | 2018-08-24 | Composition for external application for skin containing fresh ginseng steamed water obtained by vacuum drying at room temperature |
| PCT/KR2018/010029 WO2019045468A2 (en) | 2017-08-31 | 2018-08-30 | Skin external preparation composition including ginseng condensate water obtained by room temperature vacuum drying method |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310063217.6A Division CN115844788A (en) | 2017-08-31 | 2018-08-30 | Skin external preparation composition containing steamed Stichopus japonicus liquid obtained by vacuum drying at room temperature |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111201011A true CN111201011A (en) | 2020-05-26 |
Family
ID=65525905
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880066177.8A Pending CN111201011A (en) | 2017-08-31 | 2018-08-30 | Skin external preparation composition containing ginseng condensate obtained by vacuum drying at normal temperature |
| CN202310063217.6A Pending CN115844788A (en) | 2017-08-31 | 2018-08-30 | Skin external preparation composition containing steamed Stichopus japonicus liquid obtained by vacuum drying at room temperature |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310063217.6A Pending CN115844788A (en) | 2017-08-31 | 2018-08-30 | Skin external preparation composition containing steamed Stichopus japonicus liquid obtained by vacuum drying at room temperature |
Country Status (2)
| Country | Link |
|---|---|
| CN (2) | CN111201011A (en) |
| WO (1) | WO2019045468A2 (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101278296B1 (en) * | 2011-04-19 | 2013-06-25 | 박승수 | Ginsenoside collection system used vacuum and a compressive cycle |
| CN103565679A (en) * | 2012-07-19 | 2014-02-12 | 郑毅男 | Fresh ginseng extract preparation method and application of fresh ginseng extract in cosmetics |
| CN103638080A (en) * | 2013-12-12 | 2014-03-19 | 东莞市双红生物转化技术有限公司 | Preparation method for high-activity ginseng extract, product and application |
| KR20150127971A (en) * | 2014-05-08 | 2015-11-18 | 주식회사 모드리나화장품 | Modeling pack containing red ginseng or ginseng marc and manufacturing method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090129928A (en) * | 2008-06-13 | 2009-12-17 | (주)아모레퍼시픽 | Composition for applying to skin externally containing extract from flower of ginseng |
| KR101173353B1 (en) * | 2010-03-16 | 2012-08-10 | (주)풀무원홀딩스 | Ginsenoside Re increased Red Ginseng Extract and method for preparing the same |
| KR20130049649A (en) * | 2011-11-04 | 2013-05-14 | 주식회사 동구제약 | Method for manufacturing the porous red ginseng used high-vacuum art and the porous red ginseng made thereby |
-
2018
- 2018-08-30 WO PCT/KR2018/010029 patent/WO2019045468A2/en active Application Filing
- 2018-08-30 CN CN201880066177.8A patent/CN111201011A/en active Pending
- 2018-08-30 CN CN202310063217.6A patent/CN115844788A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101278296B1 (en) * | 2011-04-19 | 2013-06-25 | 박승수 | Ginsenoside collection system used vacuum and a compressive cycle |
| CN103565679A (en) * | 2012-07-19 | 2014-02-12 | 郑毅男 | Fresh ginseng extract preparation method and application of fresh ginseng extract in cosmetics |
| CN103638080A (en) * | 2013-12-12 | 2014-03-19 | 东莞市双红生物转化技术有限公司 | Preparation method for high-activity ginseng extract, product and application |
| KR20150127971A (en) * | 2014-05-08 | 2015-11-18 | 주식회사 모드리나화장품 | Modeling pack containing red ginseng or ginseng marc and manufacturing method thereof |
Non-Patent Citations (8)
| Title |
|---|
| EUNJI KIM ET AL: "The skin protective effects of compound K, a metabolite of ginsenoside Rb1 from Panax ginseng", 《JOURNAL OF GINSENG RESEARCH》 * |
| JI SEON KIM ET AL: "Enhancement of Biological Activities of Fresh Ginseng by Balanced Low Pressure Drying Processes", 《FOOD ENGINEERING PROGRESS》 * |
| 孙诗清: "《生物分离实验技术》", 28 February 2017, 北京理工大学出版社 * |
| 张彩 等: "人参化学成分和药理作用研究进展", 《食品与药品》 * |
| 张景云: "《实用美容药物学》", 31 July 2006, 中国中医药出版社 * |
| 李芳莉 等: "《实用美容美体熏浴术》", 31 January 2005, 辽宁科学技术出版社 * |
| 段金廒 等: "《中药资源化学》", 30 September 2013, 中国中医药出版社 * |
| 王昌涛 等: "《化妆品植物添加剂的开发与应用》", 30 April 2013, 中国轻工业出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019045468A3 (en) | 2019-05-09 |
| WO2019045468A2 (en) | 2019-03-07 |
| CN115844788A (en) | 2023-03-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102069712B1 (en) | Cosmetic Composition for Preventing Alopecia and Promoting Hair Growth Containing Natural Extract | |
| KR20110076043A (en) | Cosmetic composition comprising smilax china as active ingredient | |
| KR102440460B1 (en) | Composition for external application for skin containing ginseng condensed water obtained by vacuum drying at room temperature | |
| KR101787531B1 (en) | Composition for preventing hair loss comprising extracts of crude drug complex | |
| JP5616045B2 (en) | Ceramide production promoter and moisturizer | |
| KR20070095570A (en) | Methods for improving contents of physiological activated substances | |
| KR101207558B1 (en) | Cosmetic composition comprising the very high-pressure extract of red ginseng fermentation | |
| KR20170137465A (en) | Composition for improving skin condition comprising herb extracts mixture | |
| KR20170137549A (en) | Composition for improving skin condition comprising herb extracts mixture | |
| EP3689330A1 (en) | Cosmetic and pharmaceutical compositions each containing aloe extract and upland rice extract | |
| CN111201011A (en) | Skin external preparation composition containing ginseng condensate obtained by vacuum drying at normal temperature | |
| KR101856374B1 (en) | Cosmetic composition comprising steamed root extract of Acanthopanax senticosus for protecting photo-damaged skin | |
| KR102247966B1 (en) | Cosmetic Composition Containing Mixture Extracts of Phaseolus Radiatus Seed, Betula Alba Juice and Rumex Crispus Root | |
| KR101571517B1 (en) | Antiaging cosmetics containing the extract of Saururus chinensis (Lour.) Baill. with improved anti-wrinkle and antioxidant activity by Jeju Shindari fermentation method | |
| WO2020203933A1 (en) | Antiaging agent, antioxidant, antiinflammatory agent and whitening agent, and cosmetic | |
| CN115400050A (en) | Antiaging composition containing extracts of herba Saussureae Involueratae, usnea and fermented radix scrophulariae | |
| KR102540470B1 (en) | Composition for external application for skin containing fresh ginseng steamed water obtained by vacuum drying at room temperature | |
| KR102054100B1 (en) | Cosmetic composition containing fermented extract of roots | |
| KR20030004499A (en) | Cosmetic for skin whitening containing a herb extract with inhibitory activity of melanin formation | |
| KR20170136912A (en) | Composition for improving skin condition comprising herb extracts mixture | |
| JP5896618B2 (en) | Melanin production inhibitor | |
| KR102669864B1 (en) | Preparation method of bamboo extracts and cosmetic composition containing the same | |
| KR102504487B1 (en) | Glechoma hederacea var extract with skin barrier protection function and its manufacturing method | |
| KR102555941B1 (en) | Composition for skin regeneration and wound treatment comprising an absolute type essential oil derived from a Angelica polymorpha as an active ingredient | |
| EP3354255B1 (en) | Cosmetics composition containing ginsenoside as active ingredient |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |