WO2017135637A1 - Composition for promoting hair growth or for preventing hair loss, comprising extract of flower stalk of ginseng - Google Patents

Abstract

Disclosed is a composition for promoting hair growth or for preventing hair loss, comprising an extract of a flower stalk of ginseng as an active ingredient. The extract is obtained through extraction using water, C₁-C₅ alcohols or an aqueous C₁-C₅ alcohol solution. The composition can be used as a food composition, a pharmaceutical composition or a cosmetic composition. Also, the composition promotes the proliferation of hair papilla cells, and thus has an excellent hair growth promoting or hair loss preventing effect.

Description

A composition for promoting hair growth or preventing hair loss, comprising ginseng extract

The present specification relates to a composition for promoting hair growth or preventing hair loss, including ginseng flower rapeseed extract as an active ingredient.

There are only two drugs that have been approved by the FDA for hair loss treatment in 1988, for the first time to minoxidil, to finasteride, and in view of the fact that no new alternatives have been approved during the 30-year study. Hair loss treatment is still a difficult challenge.

However, minoxidil has side effects such as hirsutism when taken orally, and finasteride (finasteride) has been reported side effects such as decreased libido, decreased ejaculation, and erectile dysfunction in about 1 to 2% of patients. There is a need for safe and effective hair loss and hair loss prevention material.

Prior art literature

Korea Patent Registration No. 10-0894029

In view of the above problems, the inventors of the present invention have studied the ginseng flower stalk extract and found that the ginseng flower stalk extract has an effect of promoting hair growth or preventing hair loss.

One aspect of the present invention, to provide a composition for promoting hair growth or hair loss prevention comprising ginseng flower rapeseed extract as an active ingredient.

One aspect of the invention, the hair growth promoting or hair loss prevention composition is to provide a food, pharmaceutical or cosmetic composition.

One aspect of the present invention provides a composition for promoting hair growth or hair loss prevention comprising ginseng flower stalk extract as an active ingredient.

In one aspect of the invention, the extract is an extract extracted using water, C ₁ -C ₅ alcohol or C ₁ -C ₅ alcohol aqueous solution, provides a composition for promoting hair growth or hair loss prevention.

In one aspect of the invention, the concentration of the C ₁ -C ₅ alcohol aqueous solution is 40% to 90% (v / v), to provide a composition for promoting hair growth or preventing hair loss.

In one aspect of the invention, the C ₁ -C ₅ alcohol, hair growth using any one or two or more selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol Provided is a composition for promoting or preventing hair loss.

In one aspect of the present invention, the extract is an extract extracted using an aqueous ethanol solution of 60 to 80% (v / v) concentration, provides a composition for promoting hair growth or hair loss prevention.

In one aspect of the invention, the ginseng flower stalk is ginseng fruit is removed, provides a composition for promoting hair growth or hair loss prevention.

In one aspect of the invention, the composition provides a composition for promoting hair growth or preventing hair loss, which promotes the growth of dermal papilla cells.

In one aspect of the invention, the hair growth promoting or hair loss prevention composition provides a food, pharmaceutical or cosmetic composition.

In one aspect of the invention, the composition provides a composition formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injectable solutions.

The composition according to one aspect of the present invention has a hair loss prevention effect.

The composition according to one aspect of the present invention has a hair growth promoting effect.

The composition according to one aspect of the present invention has the effect of promoting the proliferation of dermal papilla cells.

One aspect of the invention has the effect of providing a food composition having a hair loss prevention or hair growth promoting effect.

One aspect of the present invention has the effect of providing a pharmaceutical composition having a hair loss prevention or hair growth promoting effect.

One aspect of the present invention has the effect of providing a cosmetic composition having a hair loss prevention or hair growth promoting effect.

The composition according to one aspect of the present invention is generally effective in finding the value of the part discarded by recycling the ginseng flower stem discarded during ginseng processing.

1 is a graph showing the results of the papillary cell proliferation promoting effect of the ginseng flower rapeseed extract of Examples 1 to 2 according to the present invention Experiment 1.

2 and 3 are photographs and graphs confirming hair growth through hair follicle organ culture of the ginseng flower stem extract of Example 1 according to the Inventive Example 2.

In general, the ginseng refers to the ginseng root, which is the root of ginseng, which is used as a medicinal herb or has been ingested and cooked as food. Ginseng (Panax ginseng CA Meyer) is a plant belonging to the genus Ogapiaceae ginseng, and has been used in Korea, China, and Japan for the purpose of preventing disease and extending the lifespan. In addition, anti-cancer activity, immune function control action, anti-diabetic action, anti-diabetic liver effect, cardiovascular disorders improvement, blood pressure control action, menopausal disorders improvement, anti-stress and anti-fatigue effect, antioxidant activity and anti-aging effect.

Ginseng flower stalks are not usually used as a medicinal herb, it is difficult to find a history of ingestion in the form of food. The ginseng flower stalk comes out in May-June, which is the fourth year of planting seeds, and is discarded along with the ginseng flower in June, before it blooms for the smooth growth of ginseng roots.

Ginsenoside, a representative physiologically active ingredient of ginseng, is distributed evenly in the ground and underground parts of ginseng. It is known. These differences in composition make a difference in the presence or absence of various efficacy and cause different site-specific efficacy characteristics. For example, ginseng flower extract has been shown to have an excellent effect and about 23 times more effective in the prevention and treatment of diabetes compared to ginseng root extract.

In this specification, by evaluating the hair growth efficacy of the ginseng flower stalk portion that has been discarded and not used in the past, it has been found that the ginseng stalk extract has a hair loss prevention or hair growth promotion and hair health promoting effect.

Hereinafter, the present invention will be described in detail.

One aspect of the present invention provides a composition for promoting hair growth or hair loss prevention comprising ginseng flower stalk extract as an active ingredient.

One aspect of the present invention is a method for promoting hair growth or preventing hair loss of the subject, the method provides a method comprising the step of administering an effective amount of the ginseng flower bud extract to a subject in need of promoting hair growth or preventing hair loss do.

One aspect of the invention provides the use of ginseng flower stalk extract in the preparation of a composition for promoting hair growth or preventing hair loss.

One aspect of the present invention provides a ginseng flower belt extract for promoting hair growth or preventing hair loss.

"Ginseng flower stalk" as used herein is the axial portion of the ginseng flower peduncle and flower stem.

As used herein, the term "extract" includes any material obtained by extracting a component therefrom from a natural product, regardless of the method of extraction or the kind of the component. For example, it is a broad concept including the thing which extracted the component melt | dissolved in a solvent from a natural product using water or an organic solvent, the thing obtained by extracting only the specific component of a natural product, etc. In one embodiment of the present invention, the organic solvent is not particularly limited, C1 ~ C5 lower alcohol, glycerol, ethylene, such as methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol Polyhydric alcohols such as glycol, propylene glycol, 1,3-butylene glycol, hydrocarbon solvents such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, chloroform, petroleum ether, methyl acetate, Non-polar organic solvents such as benzene, hexane, chloroform, methylene chloride, dimethyl ether, ethyl acetate and the like.

In one embodiment, the extract may be an extract extracted with water, in particular hot water. In another embodiment, the extract may be an extract extracted with C ₁ -C ₅ alcohol. In another embodiment, the extract may be an extract extracted with ethanol, but is not limited thereto.

In one aspect of the invention, the extract is an extract extracted using water, C ₁ -C ₅ alcohol or C ₁ -C ₅ alcohol aqueous solution, provides a composition for promoting hair growth or hair loss prevention.

In one aspect of the invention, the concentration of the C ₁ -C ₅ alcohol aqueous solution is 40% to 90% (v / v), to provide a composition for promoting hair growth or preventing hair loss. In one embodiment, the concentration of the C ₁ -C ₅ alcohol aqueous solution is at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 66%, at least 67%, 68% Or at least 69%, at least 70%, at least 71%, at least 72%, at least 73%, at least 74%, at least 75%, or at least 80%. In addition, the concentration of the C ₁ -C ₅ alcohol aqueous solution is 90% or less, 85% or less, 80% or less, 75% or less, 74% or less, 73% or less, 72% or less, 71% or less, 70% or less, 69 Up to 68%, up to 67%, up to 65%, or up to 60%. When the concentration of the C ₁ -C ₅ alcohol aqueous solution is within the above range, the hair loss prevention or hair growth promoting effect is excellent.

In one aspect of the invention, the C ₁ -C ₅ alcohol, hair growth using any one or two or more selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol Provided is a composition for promoting or preventing hair loss.

In one aspect of the present invention, the C ₁ -C ₅ alcohol aqueous solution, 60 to 80% (v / v) of the aqueous solution of ethanol, provides a composition for promoting hair growth or hair loss.

In one aspect of the invention, the ginseng flower rapeseed extract may be included in 0.1 to 90% by weight based on the total weight of the composition. In one embodiment, the ginseng flower blotch extract may be included 10 to 85% by weight based on the total weight of the composition. In another embodiment, the ginseng flower blotch extract may be included 20 to 80% by weight based on the total weight of the composition.

In one aspect of the invention, the ginseng flower stalk is ginseng fruit is removed, provides a composition for promoting hair growth or hair loss prevention.

In one aspect of the invention, the composition provides a composition for promoting hair growth or preventing hair loss, which promotes the growth of dermal papilla cells.

In one aspect of the invention, the hair growth promoting or hair loss prevention composition provides a food, pharmaceutical or cosmetic composition.

In one aspect of the invention, the composition provides a composition formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injectable solutions.

In one aspect of the invention, the pharmaceutical composition may further comprise, in one aspect, suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions. In one aspect the carriers, excipients and diluents that may be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

In addition, the pharmaceutical composition may be formulated in the form of powder, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral formulations, external preparations, suppositories, and sterile injectable solutions according to conventional methods. .

When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration may include tablets, pills, powders, granules, capsules and the like. Such solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, in addition to the active ingredient. In addition to simple excipients, lubricants such as magnesium styrate talc may also be included. Oral liquid preparations include suspensions, solvents, emulsions, syrups, and the like, and various excipients such as wetting agents, sweeteners, fragrances, preservatives, etc., in addition to commonly used simple diluents such as water and liquid paraffin. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, laurin, cacao butter, glycerogelatin and the like can be used.

The dosage of the extracts disclosed herein may vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration, and the duration and may be selected from the ranges commonly used in the art. In one aspect, the daily dose of the active ingredient may be 0.0001 to 1000 g / kg on a dry weight basis, and in one aspect, 0.001 to 1 g / kg.

In one aspect of the invention, the composition may be a cosmetic composition. Cosmetic compositions herein can be prepared in any formulation commonly prepared in the art, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto.

In one aspect of the present invention, the composition may be a food composition, for example, various foods, beverages, gums, teas, vitamin complexes, dietary supplements, and the like, and may be used in the form of powders, granules, tablets, capsules, or beverages. Can be. In addition to the active ingredient, the food composition of each formulation may be suitably selected by a person skilled in the art according to the formulation or purpose of use in addition to the active ingredient, and may be synergistic when applied simultaneously with other raw materials.

At this time, the amount of the active ingredient included in the food or beverage may generally include 1 to 5% by weight of the total food, if the health food composition, the health beverage composition is 0.02 to 10g based on 100ml, 0.3 in one aspect To 1 g.

In one embodiment, the composition may contain other ingredients and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, it may further include additives such as perfumes, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers and synthetic polymer materials to improve physical properties. In addition, supplementary ingredients such as water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extract may be further included. The components may be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use, and the amount of the additives may be selected within a range that does not impair the object and effect of the present invention. For example, the amount of the components added may range from 0.01-5% by weight, more specifically 0.01-3% by weight, based on the total weight of the composition.

The formulations of the compositions according to the invention may be in various forms, such as solutions, emulsions, viscous mixtures, tablets, powders, and the like, which may be administered by various methods such as simple drinking, injection, spray or squeeze.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Examples 1 to 2: preparation of ginseng flower extract

In the case of ginseng flower stalk extract, the ginseng flower stalks were collected and the fruit parts were separated and then dried in daylight or hot air to prepare ginseng flower stalk as a raw material. Thereafter, 70% ethanol (Example 1) or 10L (Example 2) of water was added to 1 kg of dried ginseng flower stalk and refluxed, filtered and concentrated under reduced pressure at 40 to 45.

The ginseng flower stalk extract obtained through the above process was used in the following experimental example.

Experimental Example 1: Evaluation of the effect of promoting dermal papilla cells

In order to confirm that the ginseng flower stalk extract of Examples 1 to 2 has an effect of preventing hair loss and promoting hair growth in a cell unit, the effect of promoting growth of dermal papilla cells was examined.

PVGDPLTt sa produced by transfecting a polyomavirus large T gene that encodes a temperature sensitive T antigen to a PVG vibrissa dermal papilla cell 6. Hereinafter, referred to as DP cells). W. Filsell (University of Cambridge, UK) was used to evaluate the donation, and evaluated the effect of promoting the growth of the dermal papilla cells of the ginseng flower extract of Examples 1 to 2.

First, DP cells were prepared in 96-well plates at a concentration of about 10 ⁴ cells / well per well. After dissolving the ginseng blotch extracts of Examples 1 and 2 in DMSO (dimethyl sulfoxide) or ethanol, respectively, serial dilution was performed with DMEM medium having a 5% FBS serum concentration so that the final concentration was 0.1, 1, or 10 ppm. It was. After adding 200 μl of the medium and the control medium to which the ginseng blotch extracts of Examples 1 to 2 were added per well, the cells were cultured in 37 and 5% CO 2 incubators for about 3 days.

50 μl of each MTT solution was added at a concentration of 2 mg / ml, and then left in the same incubator for 4 hours. After the MTT reagent was absorbed into the intracellular mitochondria, the supernatant was removed, and 150 μl of DMSO was added, followed by stirring for 10 minutes to uniformly dissolve promazan dye. Then, using an ELISA reader, O.D. The value was measured, and the cell number was measured according to a standard curve prepared in advance. Based on the number of cells measured, percent of papillary cell proliferation promoting ability was calculated.

The dermal papilla cell growth promoting ability according to the above experiment is shown in FIG. 1.

As shown in Figure 1, the ginseng flower extract of Example 1 (70% ethanol extraction) and Example 2 (water extraction) was confirmed to have a capacity-dependent excellent dermal papilla cell proliferation effect. Therefore, the ginseng flower stalk extract was found to have an excellent effect of promoting hair papilla cell proliferation, preventing hair loss, promoting hair growth, and further promoting hair health.

Experimental Example 2 Evaluation of Hair Growth Effect Through Hair Follicle Organ Culture

In order to confirm that the ginseng flower stalk extract of Example 1 (70% ethanol extract) is effective in preventing hair loss and promoting hair growth in the tissue unit, hair growth effect was examined through culturing hair follicle organs.

The experiment was carried out according to the following steps.

-Separation of human hair follicles: Prepare laryngeal biopsy tissue provided by Dankook University Dermatology in cold PBS. Trimming the hair follicle (Follicular unit) and cut to just below the sebaceous gland (Fig. 2).

Human hair follicle sample information (# 1: 52y male, # 2: 57y male)

-10 to 15 follicular units per test group

-Hair follicle culture: After dispensing 500ul medium into a 24well plate, culture 6 (x2 wells) samples per well,

-Culture medium: William's E media with L-glutamine (2mM), hydrocortisone (10ng / ml), antibiotics (1%), insulin (10㎍ / ml) fungizone (0.1%), medium exchange every 3 days

-Material treatment: After dissolving the ginseng flower extract of Example 1 in DMSO (dimethyl sulfoxide) or ethanol, respectively, serial dilution was carried out with the culture medium so that the final concentration was 10, 20 µg / ml.

Growth length measurement: hair follicles were photographed using a dissection microscope and Image J program was used to measure growth length on Day 2, 5, 7 findings. Representative images of hair follicle length growth on Day 5 of the hair follicle sample of # 2 were taken and shown in FIG. 2. In addition, a graph measuring the hair follicle growth length on Days 2 and 5 of # 2 hair follicle sample is shown in FIG. 3.

As can be seen in Figures 2 and 3, as a result of evaluation through human hair follicle tissue culture evaluation system, it was found that there is an effect of promoting hair follicle length growth when 20ppm ginseng flower extract of Example 1 is treated. Therefore, the ginseng flower stalk extract was found to have an excellent effect of promoting hair follicle length growth, preventing hair loss, promoting hair growth, and further promoting hair health.

Claims (9)

1. A composition for promoting hair growth or preventing hair loss, including ginseng flower rapeseed extract as an active ingredient.
2. The method of claim 1,

   The extract is an extract extracted using water, C ₁ -C ₅ alcohol or C ₁ -C ₅ alcohol aqueous solution, hair growth promoting or hair loss prevention composition.
3. The method of claim 2,

   The concentration of the C ₁ -C ₅ alcohol aqueous solution is 40% to 90% (v / v), hair growth promoting or hair loss prevention composition.
4. The method of claim 2,

   The C ₁ -C ₅ alcohol, methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol using any one or more selected from the group consisting of, hair growth promoting or hair loss prevention composition.
5. The method of claim 2,

   Wherein the extract is an extract using an aqueous ethanol solution of 60 to 80% (v / v) concentration, hair growth promoting or hair loss prevention composition.
6. The method of claim 1,

   The ginseng flower stalk is that ginseng fruit is removed, hair growth promoting or hair loss prevention composition.
7. The method according to any one of claims 1 to 6,

   The composition is to promote the growth of dermal papilla cells, hair growth promoting or hair loss prevention composition.
8. The method according to any one of claims 1 to 6,

   The hair growth promoting or hair loss preventing composition is a food, medicine or cosmetic composition.
9. The method of claim 8,

   The composition is formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injectable solutions.

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