KR101274868B1 - Composition for Preventing Hair Loss and Improving Hair Condition Comprising an Extract of Asidian Tunic - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing hair loss and improving hair growth comprising the shell extract as an active ingredient. Sea urchin extract of the present invention inhibits 5α-reductase activity, and thus has an excellent effect of inhibiting the production of DHT, which causes hair loss, the composition of the present invention comprising it as an active ingredient prevents hair loss and hair growth. It can be usefully used for improvement. In particular, such as the shellfish extract is a natural substance, because it corresponds to an edible substance, the composition of the present invention comprising it as an active ingredient has a safe advantage even for long-term use.

Description

Composition for Preventing Hair Loss and Improving Hair Condition Comprising an Extract of Asidian Tunic}

The present invention relates to a composition effective for preventing hair loss and improving hair growth, and more particularly, to a hair loss preventing and hair growth improving cosmetic composition comprising a sea shell extract as an active ingredient.

The causes of hair loss have been discussed in the role of male hormones in organs such as hair roots and sebaceous glands, lowering blood flow of hair follicles, scalp abnormalities caused by bacterial reproduction or excessive sebum secretion, genetic factors, and aging. However, the definite cause of hair loss has not yet been clarified. Recently, people who are suffering from alopecia are increasing and the age group is lowering.

There are no clear effects on the treatment of alopecia, and there are some prescriptions for taking hair loss such as diagnosis of neonatal volume in Dongyoungbo, and massage of sesame oil etc. on hair loss site, And the method of stimulating specific blood circulation. However, it is known that there are many individual differences in the effect, and there is not yet reported a new drug substance which can be generally applied.

The human body has more than 1.3 million hairs and more than 100,000 hairs. These hairs go through three cycles of anagen, catagen, and telogen, and each hair is run in an independent cycle. Hair loss refers to a condition in which hair or hair is less or less than normal due to some cause, and is classified into general hair loss phenomenon (telogen effluvium) in which hair falls out of a resting state and anagen effluvium, which is abnormal hair loss. The latter is commonly referred to as hair loss.

It is well known that testosterone, a male hormone, affects hair loss, especially with regard to hair loss. The effect of androgen on hair loss is that testosterone binds to the 5alpha-reductase enzyme in the body to produce dihydroxytestosterone (DHT). It binds to androgen receptors in hair follicles and has a dominant effect on inhibiting hair follicle formation in hair follicles. Therefore, studies are being actively conducted to develop a hair loss preventing substance using an inhibitor of 5α-reductase enzyme.

Currently commercially available hair regrowth agents include minoxidil and trichosaccharide from Crinos, Co., USA, but there are no obvious effects and side effects. . Finasteride, developed by Merck, is known to inhibit the activity of 5α-reductase, an enzyme that acts on male hormone testosterone metabolism in hair follicles, but it can also cause depression. The problem is that long-term treatment is required. In addition, there are Crescina and Rogaine, but only when the hair follicle is alive on the normal scalp before the hair loss disease is fully progressed. As described above, various types of conventional hair regrowth agents have been attempted to help hair growth for the purpose of promoting blood circulation of the scalp and nourishing hair roots. However, the current toxic and side effects are severe and their effects are insufficient.

In recent years, research on the treatment of hair loss has been actively conducted using various new substances extracted from natural products, which have no toxicity and do not cause side effects even in long-term use.

On the other hand, the sea squirrel is an organism belonging to the side seagrasses of the seaweed river, which are native to the marine world, and the sea squirt is non-toxic, edible and mass-produced through aquaculture. However, the meat inside the sea squirt is used for food, but the shell is usually thrown away. At this time, since the shell is hard and not easily broken down, it is a cause of environmental pollution.

Therefore, the present inventors, while seeking to find a natural material excellent in inhibiting the activity of 5α-reductase activity without side effects in the living body, the results of experiments pharmacological effect by paying attention to the sea shells currently being treated as waste, The present invention was completed by confirming that the sea snail extract inhibits 5α-reductase activity, thereby effectively inhibiting the production of DHT, which causes hair loss.

Accordingly, an object of the present invention is to provide a cosmetic composition for preventing hair loss, promoting hair growth, and improving scalp, having an effect of inhibiting 5α-reductase activity without causing side effects in the living body, thereby inhibiting the production of DHT, which causes hair loss. It is.

In order to achieve the object of the present invention as described above, the present invention provides a cosmetic composition for preventing hair loss, promoting hair growth and scalp improvement comprising an extract of the sea shell as an active ingredient.

In one embodiment of the present invention, the content of the sea shell extract may be included as 0.00001-30% by weight relative to the total weight of the composition.

In one embodiment of the present invention, the sea shell extract may be obtained by extraction with water or an organic solvent.

In one embodiment of the present invention, the sea shell extract may have the effect of lowering the amount of dihydrotestosterone (DHT) production through the inhibition of the activity of 5 alpha-reductase.

In one embodiment of the present invention, the cosmetic composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizer cream, Hair Massage Cream, Hair Wax, Hair Aerosol, Hair Pack, Hair Nutrition Pack, Hair Soap, Hair Cleansing Foam, Hair Oil, Hair Dryer, Hair Preservative, Hair Dye, Hair Wave, Hair Decolorant, Hair Gel, Hair Glaze, Hair Dressing Er, hair lacquer, hair moisturizer, hair mousse and hair spray may be a formulation selected from the group consisting of.

In another aspect, the present invention provides a pharmaceutical composition for preventing hair loss, promoting hair growth and scalp improvement comprising the shell extract as an active ingredient.

In one embodiment of the present invention, the pharmaceutical composition is a skin external preparation selected from the group consisting of creams, gels, patches, sprays, ointments, warnings, lotions, linen preparations, pasta preparations and cataplasma preparations. It may be in the form of a composition.

In another aspect, the present invention provides a health functional food for hair loss prevention, hair growth promotion and scalp improvement comprising the shellfish extract as an active ingredient.

In one embodiment of the present invention, the food is selected from the group consisting of beverage, meat, chocolate, foods, confectionery, pizza, ram noodles, gums, candy, ice cream, alcoholic beverages, .

Sea urchin extract of the present invention inhibits 5α-reductase activity, and thus has an excellent effect of inhibiting the production of DHT, which causes hair loss, the composition of the present invention comprising it as an active ingredient prevents hair loss, hair growth It can be usefully used for palpation and scalp improvement. In particular, such as the shellfish extract is a natural substance, because it corresponds to an edible substance, the composition of the present invention comprising it as an active ingredient has a safe advantage even for long-term use.

Figure 1 is a graph measuring the amount of DHT production according to the application of the shellfish shell extract of the present invention in vitro.
Figure 2 shows a photograph of the process of hair growth after applying the shellfish shell extract of the present invention to the depilated mouse.

The present invention relates to a novel use of the shellfish shell extract, characterized by providing a composition for preventing hair loss, promoting hair growth and scalp improvement comprising the shellfish shell extract as an active ingredient.

Sea squirt is a non-toxic, edible, marine organism that is produced in large quantities through aquaculture. The meat inside of the sea squirt is used for food, but the shell is usually thrown away. At this time, the shell is hard and cannot be easily broken down.

The present invention confirms that the shellfish extract currently being treated as waste inhibits 5α-reductase activity, thereby inhibiting the production of DHT, which causes hair loss, thereby preventing hair loss, promoting hair growth, and scalp. It was the first time that it was effective for improvement.

Therefore, the present invention can provide a cosmetic composition for preventing hair loss, promoting hair growth, and improving the scalp, including the sea shell extract as an active ingredient.

The sea squirt extract according to the present invention can be obtained by extraction and separation from nature using extraction and separation methods known in the art, the 'extract' as defined in the present invention is the sea squirt shell using an appropriate solvent It is extracted from, and includes, for example, all the hot water extract of the sea shell, polar solvent soluble extract or non-polar solvent soluble extract.

As a suitable solvent for extracting the extract from the sea shell, any solvent may be used as long as it is acceptable in the art, and water or an organic solvent may be used. Examples of the solvent include alcohols having 1 to 4 carbon atoms, acetone, ether, and the like, including purified water, methanol, ethanol, propanol, isopropanol, butanol, Various solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. But is not limited to.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation in the preparation method of the sea squirt extract of the present invention, any known method can be used.

For example, the sea squirt shell extract included in the composition of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying. have. The primary extract may be further purified using various chromatography such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, and the like. You can also get

Therefore, in the present invention, the shellfish extract is a concept including all the extracts, fractions and purified products obtained at each step of extraction, fractionation or purification, their dilutions, concentrates or dried products.

According to a specific embodiment of the present invention, the production of the sea shell extract of the present invention is carried out as follows.

The sea squirt extract of the present invention comprises the steps of: a) hot water extraction after mixing water on the scab; b) centrifuging the hot water extract to discard the precipitate to obtain a supernatant; c) concentrating the supernatant to form a concentrate; d) removing the protein from the concentrate; e) centrifuging the protein removal concentrate to obtain a supernatant; And f) filtering the supernatant obtained through step e) and freeze drying the unfiltered residue.

The amount of water mixed in the step a) is preferably 5 to 10 times based on the weight of the sea shell, the hot water extraction temperature is preferably 100 to 150 ° C, the hot water extraction time is preferably 2 to 5 hours, and high pressure. Hot water extraction is recommended under In the following examples of the present invention, the shellfish and water were added to a high pressure steam cooker (Dongnam Industry, DN-1001) to extract hot water.

Centrifugation in step b) may be centrifuged for 10 to 30 minutes at 3,000rpm to 5,000rpm.

The concentrate in step c) can be obtained by concentrating the supernatant obtained through step b) until Briggs 10. In the following example of the present invention was concentrated using a rotary vacuum evaporator (Hahn Shin Scentific, Model No. 20sp).

The removal of protein in step d) may be performed by adding ammonium sulfate to the concentrate obtained through step c) and leaving it in a refrigerated state. In the following examples of the present invention, 30 to 70% of ammonium sulfate was added to the concentrate and kept in a refrigerated state of 0.01 to 10 ° C. for 24 hours.

Centrifugation in step e) may be centrifuged for 10 minutes to 30 minutes at 3,000rpm to 5,000rpm.

The step f) is a step of filtering the supernatant obtained through step e) and freeze drying the unfiltered balance, preferably dialysis the supernatant obtained through step e) with distilled water, and then It may be made through the process of filtration, and the freeze-dried residue of the unfiltered residue. In the following example of the present invention, the supernatant was dialyzed with distilled water until the salt content became 0.1% or less, and filtered through a 10,000 Da ultrafiltration membrane.

The composition of the present invention comprising such a sea shell extract as an active ingredient may be a cosmetic composition.

When the composition of the present invention is made of a cosmetic composition, the composition of the present invention may include not only the above-described sea shell extract, but also components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins , Conventional adjuvants such as pigments and perfumes, and carriers.

In addition, the composition of the present invention can be used in addition to the above-mentioned sea buckthorn extract, a wool or hair-grown agent that has been conventionally used as long as it does not impair the effect of hair growth prevention and hair improvement by the sea buckthorn extract.

The cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art, and examples thereof include a hair tonic, a hair conditioner, a hair essence, a hair lotion, a hair nutrition lotion, a hair shampoo, a hair conditioner, Hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave, hair cosmetic cream, hair moisturizing cream, But are not limited to, various types such as hair removers, hair bleaching agents, hair gels, hair glazes, hair dressers, hair lacquers, hair moisturizers, hair mousses and hair sprays.

According to a preferred embodiment of the present invention, the content of the sea shell extract of the present invention is 0.00001-30% by weight, preferably 0.5-20%, more preferably 1.0-10% by weight based on the total weight of the composition. When the content of the shellfish shell extract is less than 0.00001% by weight, hair loss prevention and hair growth improvement effect by the shellfish shell extract is greatly reduced, when the content exceeds 30% by weight may cause skin irritation, and the problem of formulation have.

On the other hand, the cosmetic composition according to the present invention may be formulated by stabilizing the inclusion of the sea shell shell extract inside the nanoliposomes. When the extract is contained inside the nanoliposomes, the components of the extract can be stabilized to solve problems such as precipitation formation, discoloration, and odor when formulated, and can increase the solubility and transdermal absorption of the components, thereby improving efficacy expected from the extract. Maximum expression can be achieved.

In the present invention, nanoliposome refers to a liposome having a typical liposome form and having an average particle diameter of 10 to 500 nm. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposomes is 50 ~ 300nm. When the average particle diameter of the nanoliposome is more than 300 nm, improvement of skin penetration and improvement of formulation stability is very weak among technological effects to be achieved in the present invention.

Nanoliposomes used to stabilize the extract according to the present invention may be prepared by a mixture comprising polyols, oily ingredients, surfactants, phospholipids, fatty acids and water.

The polyol used in the nanoliposome of the present invention is not particularly limited and is preferably a polyol such as propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isopropylene glycol, pentylene glycol, erythritol, , Sorbitol, and mixtures thereof. The amount used is 10 to 80% by weight, preferably 30 to 70% by weight based on the total weight of the nanoliposomes.

The oil component used in the preparation of the nanoliposome of the present invention may be a variety of oils known in the art, preferably hydrocarbon-based oils such as hexadecane and paraffin oil, synthetic oils of esters, Silicone oils such as dimethicone and cyclomethicone, animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil, ethoxylated alkyl ether oils, propoxylated alkyl ether oils ₄₀ is a fatty alcohol, and mixtures thereof -, phytosphingosine, sphingosine, and scan non-ping the same scan pinggo cannabinoid lipid, cholesterol side-by celebrity, sitosterol cholesteryl sulfate, cholesteryl sulfate, cytokines, C _10. The amount may be 1.0 to 30.0% by weight based on the total weight of nanoliposomes, preferably 3.0 to 20.0% by weight.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art. For example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants can be used. Preferred are anionic surfactants and nonionic surfactants. Specific examples of anionic surfactants include alkyl acyl glutamates, alkyl phosphates, alkyl lactylates, dialkyl phosphates and trialkyl phosphates. Specific examples of nonionic surfactants include alkoxylated alkylethers, alkoxylated alkylesters, alkylpolyglycosides, polyglyceryl esters and sugar esters. Particularly preferred surfactants are polysorbates belonging to nonionic surfactants. The amount thereof may be 0.1 to 10% by weight, preferably 0.5 to 5.0% by weight, based on the total weight of the nanoliposome.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are amphoteric affinity lipids, and include natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg, Dipalmitoylphosphatidylcholine or hydrogenated lecithin), preferably lecithin. In particular, unsaturated lecithin or saturated lecithin derived from nature extracted from soybean or egg yolk are preferred. Typically, lecithin derived from nature has 23-95% phosphatidylcholine and 20% or less phosphadidylethanolamine. In the preparation of the nanoliposomes of the present invention, the amount of phospholipid used is 0.5 to 20.0% by weight, preferably 2.0 to 8.0% by weight, based on the total weight of the nanoliposomes.

Fatty acid to be used in nano-liposome preparation of the present invention is a higher fatty acid, preferably a C ₁₂ - ₂₂ as a saturated or unsaturated fatty acid of the alkyl chain, e.g., lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid It includes. The amount may be 0.05 to 3.0% by weight based on the total weight of the nanoliposomes, preferably 0.1 to 1.0% by weight.

Water used in the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which may be 5.0 to 40% by weight relative to the total weight of nanoliposomes.

The preparation of nanoliposomes can be accomplished through a variety of methods known in the art, but most preferably is made by applying a mixture comprising the ingredients to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg, pressure, frequency, etc.) according to the desired particle size, and preferably 1 to 5 times high pressure homogenizer under a pressure of 600 to 1200 bar. Nanoliposomes can be prepared by passing through.

Hair loss prevention, hair growth promotion and scalp improvement cosmetic composition according to the invention may contain 0.1 ~ 20.0% by weight based on the nanoliposome filling weight, more preferably 1.0 ~ 10.0% by weight for stabilizing the formulation. .

The composition of the present invention may also be a pharmaceutical composition comprising the sea squirt extract as an active ingredient.

The pharmaceutical composition of the present invention can be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, A lubricant or a flavoring agent can be used.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

Formulation forms of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include but are not limited to natural and synthetic gums such as starch, gelatin, glucose or beta-lactose, corn sweeteners, acacia, trackercance or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like. Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile water and sterile water suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

In one embodiment of the present invention, the sea squirt extract of the present invention may be included in 0.00001 to 30% by weight based on the total weight of the composition.

In one embodiment of the present invention, the pharmaceutical composition of the present invention may be an external skin composition.

The external skin pharmaceutical composition of the present invention is a skin external preparation having the effect of preventing hair loss, promoting hair growth and improving the scalp, and includes cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma. It may be prepared and used as a pharmaceutical composition in the form of external preparations for skin, but is not limited thereto.

The composition of the present invention may also be a food composition, which may contain various flavors or natural carbohydrates as additional ingredients in addition to the sea urchin extract as an active ingredient, as in a conventional food composition.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. The aforementioned flavoring agents can advantageously be used natural flavoring agents (tautin), stevia extracts (for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.).

The food composition of the present invention may be formulated in the same manner as the pharmaceutical composition, used as a functional food, or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolates, foods, confectionery, pizza, ram noodles, other noodles, gums, candy, ice cream, alcoholic beverages, vitamin complexes, .

In addition, the food composition is a nutrient, vitamins, minerals (electrolytes), synthetic flavors and natural flavoring agents, colorants and neutralizing agents (cheese, chocolate, etc.), pectic acid and its Salts, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks and the like. In addition, the food composition of the present invention may contain a fruit flesh for producing natural fruit juice and fruit juice beverage and vegetable beverage.

Sea urchin shell extract as an active ingredient of the present invention is a natural substance with little toxicity and side effects, so it can be used with confidence even during long-term use for the purpose of preventing hair loss, promoting hair growth and improving the scalp.

The present invention also provides a health functional food for hair loss prevention, hair growth promotion and scalp improvement comprising the sea shell extract as an active ingredient.

The health functional food of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, circles, etc. for the purpose of preventing hair loss, promoting hair growth and improving scalp.

In the present invention, "health functional food" refers to a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to the Health Functional Food Act No. 6767, and nutrients for the structure and function of the human body. It is meant to be consumed for the purpose of regulating or obtaining a useful effect for health use such as physiological action.

The health functional food of the present invention may include a conventional food additive, and the suitability as a food additive, unless otherwise specified, in accordance with the General Regulations of the Food Additives and General Test Methods approved by the Food and Drug Administration, etc. Judging by the standards and standards.

Examples of the items listed in the "Food Additive Reduction" include chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as dark blue pigment, licorice extract, crystalline cellulose, high color pigment and guar gum; L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar coloring preparations and the like.

For example, the health functional food in the form of tablets is a mixture of excipients, sea urchin shell extract, an active ingredient of the present invention with excipients, binders, disintegrants and other additives by granulating in a conventional manner, and then compressed with a lubricant and the like. Or the mixture can be directly compression molded. In addition, the health functional food in the form of tablets may contain a mating agent and the like as necessary.

Hard capsules among the health functional foods in the form of capsules may be prepared by filling a mixture of the sea urchin shell extract, which is the active ingredient of the present invention, with an additive, such as an excipient, in a conventional hard capsule. The mixture mixed with the additive of may be prepared by filling in a capsule base such as gelatin. The soft capsule agent may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, as necessary.

The health functional food in the form of a cyclic form may be prepared by molding a mixture of the sea urchin shell extract, an active ingredient of the present invention, an excipient, a binder, a disintegrant, and the like by a conventionally known method. It may be avoided, or the surface may be coated with materials such as starch, talc.

The health functional food in the form of granules can be prepared in the form of granules by mixing known mixtures of the shellfish extract, the excipient, the binder, the disintegrant, and the like, which are the active ingredients of the present invention, and, if necessary, flavoring agents and copulation agents. And the like.

The sea squirt extract of the present invention inhibits 5α-reductase activity as confirmed in the following examples, thereby effectively inhibiting the production of DHT that causes hair loss, and thus, the present invention comprising it as an active ingredient. Dietary supplements are effective in preventing hair loss, promoting hair growth and improving scalp.

The health functional food may be a beverage, a meat, a chocolate, a food, a confectionery, a pizza, a ramen, a noodle, a gum, a candy, an ice cream, an alcoholic beverage, a vitamin complex and a health supplement food.

In another aspect, the present invention provides a use of the composition comprising the sea squirt extract as an active ingredient for the production of cosmetics, medicine or food for preventing hair loss, promoting hair growth and improving the scalp. The composition of the present invention comprising the above-mentioned sea buckthorn extract as an active ingredient can be used for the manufacture of cosmetics, medicine or food for preventing hair loss, promoting hair growth and improving scalp.

In another aspect, the present invention provides a method for preventing or treating hair loss, comprising administering to the mammal the shellfish extract.

The term "mammal " as used herein refers to a mammal that is the subject of treatment, observation or experimentation, preferably a human.

The term "therapeutically effective amount " as used herein refers to the amount of active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, as contemplated by a researcher, veterinarian, physician or other clinician, The amount that induces the relief of the symptoms of the disease or disorder being treated. It will be apparent to those skilled in the art that the therapeutically effective dosages and frequency of administrations for the active ingredients of the present invention will vary depending on the desired effect. The optimal dosage to be administered can therefore be readily determined by those skilled in the art and will depend upon the nature of the disease, the severity of the disease, the amount of active and other ingredients contained in the composition, the type of formulation, and the age, , Sex and diet, time of administration, route of administration and rate of administration of the composition, duration of treatment, concurrent administration of the drug, and the like. In the treatment method of the present invention, in the case of adults, it is preferable that the dose of 1 mg / kg to 250 mg / kg is administered once to several times a day of the sea shell extract of the present invention.

In the treatment method of the present invention, the composition comprising the extract of the sea squirt shell of the present invention as an active ingredient may be administered by oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes. It may be administered in a conventional manner.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Sea Shell Extract

In order to prepare the sea squirt extract, the sea squirt shell was first prepared by washing with running water until clear water came out. The sea urchin shell thus prepared was placed in a high pressure steam cooker (Dongnam Industrial Co., DN-1001) and water was added 5-10 times the amount of the sample, followed by steaming at 125 ° C. for 2-5 hours. Thereafter, the mixture was allowed to stand at room temperature and then centrifuged at 3,000 rpm for 20 minutes (Hanil Scentific Industrial, MF 600 PLUS, Large Capacity Centrifuge). After this centrifugation, the precipitated precipitate was discarded, and the supernatant was collected and concentrated using a rotary evaporator (Hahn Shin Scentific, Model No. 20sp) until brix 10 was obtained. Thereafter, 30-70% of ammonium sulfate was added to remove the protein contained in the concentrate, and left for 24 hours in a refrigerated state (0-10 ° C). This was centrifuged at 3,000 rpm for 20 minutes, and the supernatant obtained through centrifugation was dialyzed with distilled water until the salt content became 0.1% or less. The solution was filtered through a 10,000 Da ultrafiltration membrane, and the unfiltered residue was freeze-dried (F-540, Tokyo Rikakikai Co., Ltd, Tokyo, Japan) to obtain the sea shell extract of the present invention.

< Example  2>

Toxicity Evaluation of Sea Urchin Extracts of the Present Invention

To determine the toxicity of the sea squirt extract of the present invention, the toxicity test was conducted with RAW 264.7 cells. In order to determine the toxicity of the shellfish shell extract of the present invention prepared in Example 1 was treated in the medium by concentration (1μg / ml, 50μg / ml, 100μg / ml, 200μg / ml).

As a result, as shown in Table 1, it was confirmed that the toxicity does not appear at all concentrations.

sample Cell viability (%) Control group 100.0 ± 4.2 Sea urchin extract 1 ug / ml 116.3 ± 3.7 Sea urchin extract 50 ug / ml 124.2 ± 1.5 Sea urchin extract 100 ug / ml 124.7 ± 6.5 Sea Urchin Extract 200 ug / ml 124.4 ± 7.2

< Example  3>

According to the application of the sea squirt shell extract of the present invention Dermal papilla cells  Playback Effect Analysis

Proliferation rate of dermal papilla cells after applying the shellfish extract of the present invention to human follicle dermal papilla cells in order to verify whether the extract of the present invention prepared in Example 1 has an effect of regenerating dermal papilla cells Was measured.

The dermal papilla cells were tested by purchasing primary cells from human follicle dermal papilla (C-12071) from Promocell GmbH (Heidelberg, Germany). As a medium for cell culture, follical dermal papilla cell growth medium (C-26501) was purchased from the same company as the cell company. Incubated with medium containing 10% calf serum (FBS; Gibco). The cells were dispensed at 2000 cells / well in 96-well plates, and after 24 hours, the samples were incubated with each concentration.

The survival rate of the cells was treated with Promega MTS [3- (4,5-dimethyl-thiazol-2-yl) -5-carboxymethoxy-phenyl-2- (4-sulfophenyl) -2H-tetrazolium] reagent for 1 hour. Absorbance was measured at 490 nm with a microplate reader (Perkin Elmer 1420 Multilabel Counter VICTOR ™, USA). Cell viability was expressed as absorbance of the sample treated group compared to the control.

As a result, as shown in Table 2, it was confirmed that the growth rate of the dermal papilla cells in a concentration-dependent manner over time in the group treated with the sea squirt extract of the present invention.

Proliferation rate for dermal papilla cells sample Proliferation rate (%) 1 day 3 days 5 days Control group 100.0 ± 2.4 100.0 + - 3.7 100.0 ± 3.6 Sea urchin extract 1 ug / ml 99.9 ± 6.1 104.6 ± 2.0 101.2 ± 11.5 Sea urchin extract 50 ug / ml 102.1 ± 2.2 101.8 ± 4.3 103.7 ± 7.0 Sea urchin extract 100 ug / ml 103.7 ± 2.2 106.3 ± 2.3 * 121.3 ± 7.9 ** Sea Urchin Extract 200 ug / ml 103.7 ± 3.0 110.8 ± 5.4 ** 127.2 ± 3.9 **

< Example  4>

According to the application of the sea squirt shell extract of the present invention DHT  produce Inferior  Measure

Testosterone, a male hormone, binds to the 5alpha-reductase enzyme in the body to produce dihydroxytestosterone (hereinafter referred to as 'DHT'), which binds to androgen receptors in the hair roots. It has a dominant effect on inhibiting hair follicle production. Therefore, in this experiment, the effect of preventing hair loss was examined by measuring the inhibition of DHT production.

6 g of liver tissues of SD rats were homogenized with 12 ml PBS, and 1 ml / ml of the shellfish extract of the present invention was treated with 1 ml of the homogenate, followed by reaction at 37 ° C. for 15 minutes. After 15 minutes, 1 ml of extraction buffer (50 mM Na2HPO4, 0.25 M sucrose) was added to the homogenate and vortexed. After centrifugation at 1,500g at 4 ° C, the supernatant was taken and used as a sample. At this time, it was treated as a negative control that was not treated with the sea squirt extract, and as a positive control, finasteride, a drug sold as a 5-alpha-reductase inhibitor, instead of the sea squirt extract of the invention Used.

In the present invention, ELISA kit for DHT (Uscn Life Science, China) was used to measure the degree of DHT production. 100 µl of the standard and the sample were dispensed into a 96 well plate enclosed in the kit, and then reacted at 37 ° C. for 2 hours. After 2 hours, the solution was removed, and 100 µl of detection reagent A was dispensed thereto. After the reaction at 37 ° C. for 1 hour, the mixture was washed three times with wash buffer. 100 μl of detection reagent B was dispensed and reacted at 37 ° C. for 1 hour. After the reaction was completed, the supernatant was removed, washed five times with wash buffer, 90 μl of substrate solution was added, the plate was wrapped with foil, and reacted at 37 ° C. for 15 minutes. 50 μl of stop solution was added to terminate the reaction, and the absorbance was measured at 450 nm.

As a result, as shown in Figure 1, compared with the control group in the experimental group treated with the bark shell extract of the present invention was found to show a significantly higher DHT production inhibitory ability in a concentration-dependent manner.

< Example  5>

Measurement of hair growth effect using mouse

In order to determine the hair growth efficacy of the sample using a mouse, a lotion was first prepared as shown in the following table.

Preparation of Solution Containing Sea Urchin Extract of the Present Invention Name of sample water Propylene glycol Seashell Extract Preservative (Gsilk) Negative control group 97 3 0 0.3 Positive control group
(Minoxidil) - - - - Examination group 1 96.6 3 0.1 0.3 Experiment 2 95.7 3 One 0.3

Purified water and propylene glycol were added to the sea squirt extract (0.1 g, 1 g) of the present invention, and a small amount of preservative (Gsilk-P350, GFC Co. Ltd, Korea) was added and dissolved at 1,200 rpm at 40-60 ° C. for 20 minutes. After standing at room temperature, each filtrate filtered with 0.25um filter paper was used as a 0.1% sea urchin extract extract (Experimental Group 1) and a 1% sea urchin extract extract (Experimental Group 2). As a positive control, commercially available 5% minoxidil (Regrowth hair restorer) was used. For the experiment, black mouse C57BL 4-7 weeks old was divided into 6 dogs per group, the hair of the mouse was removed with a hair remover, and the back hair was completely removed with a hair removal cream. 100 μl of the sample was applied to the back area once daily at a certain time every day, and the photograph was taken once a week. Each sample was taken at the same time once a week while each sample was applied daily to the hair removal site of each mouse.

As a result, as shown in FIG. 2, as the experiment was conducted in all groups, the body surface color changed from pink to black, which is the most active hair growth in the telogen where the hair cycle stops or rests. It can be seen that the process of turning into a hair growth phase (anagen) that is a cycle. Two weeks after the sample application, the hair began to grow slowly in some groups, especially in the minoxidil-coated group, which was positive control, and began to grow in some of the mouse embryos. As it turned to color, hair began to grow. As observed in the photograph on day 21 of the application, the minoxidildo group was able to confirm the hair growth by covering all the embryos completely in all mice, and in the group 2, hair growth was observed in more than half of the mice. In the photograph on the 28th day, the two groups' back hair grew to a level similar to that of the minoxidil-treated PC group.

Therefore, considering the results of this study, group 2 shows a slower effect than that of minoxidil, which is a positive control group, but minoxidil, which is commercially available by pulling the anagen, a hair growth cycle, in which hair growth occurs when a period of about 4 weeks is applied. The effect was similar to that of hair growth. In addition, minoxidil was used as a 5% solution, but the sea urchin extract of the present invention showed a similar effect at a concentration of 1% was confirmed to have a very excellent hair growth effect.

So far I looked at the center of the preferred embodiment for the present invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (9)

A cosmetic composition for preventing hair loss and improving hair growth, comprising a sea squirt extract as an active ingredient. The method of claim 1,
The content of the shellfish shell extract is a cosmetic composition, characterized in that it comprises 0.00001-30% by weight relative to the total weight of the composition. The method of claim 1,
The sea urchin extract is a cosmetic composition, characterized in that obtained by extraction with water or an organic solvent. The method of claim 1,
The sea urchin extract is a cosmetic composition, characterized in that it has an inhibitory effect on the production of dihydrotestosterone (DHT) through the inhibition of the activity of 5 alpha-reductase. The method of claim 1,
The cosmetic composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, hair massage cream, hair wax, hair aerosol , Hair pack, nourishment pack, hair soap, hair cleansing foam, hair oil, hair drier, hair preservative, hair dye, hair wave, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, Cosmetic composition, characterized in that the formulation is selected from the group consisting of hair mousse and hair spray. A pharmaceutical composition for preventing hair loss and promoting hair growth, comprising a sea squirt extract as an active ingredient. The method according to claim 6,
The pharmaceutical composition is a composition for external skin preparations selected from the group consisting of creams, gels, patches, sprays, ointments, warnings, lotions, linings, pastas and cataplasmas as external skin preparations. Pharmaceutical composition. delete delete

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