KR20080035453A - Manufacturing method of ginseng fruit and ginseng spray plenty ginsenoside quantity - Google Patents

Abstract

A method of manufacturing fruit and flower stalk of ginseng having high contents of ginsenoside is provided which increases the content of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re and other ginsenosides. Fruit and flower stalk of ginseng are treated with light, steamed one or more times, dried and then soaked in a solution containing one or more ginsenoside hydrolysates selected from alpha-glucosidase, alpha-Rhamnosidase and soyasapogenol B. After the soaking process, the fruit and flower stalk can be additionally treated with any one of ultrasonic treatment, low-frequency treatment, electric field treatment and magnetic field treatment. The light is selected from infrared rays, visible rays and ultraviolet rays.

Description

Manufacturing method of ginseng fruit and ginseng vase with high content of ginsenosides {Manufacturing method of ginseng fruit and ginseng spray plenty ginsenoside quantity}

The present invention relates to a ginseng fruit with a high content of ginsenoside components and a method for producing a ginseng vase, and more specifically, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re and others. It relates to a ginseng fruit and ginseng vase manufacturing method with a high content of ginsenosides.

Ginseng is a perennial semi-negative plant and belongs to Ogapiaceae, and its efficacy is widely known in various documents in China and Korea, and it has been used as a herbal medicine in many fields since ancient times.

Ginseng has been widely studied in modern times, and ginseng is known to contain a large amount of ginsenosides (Saponin). In addition to the medicinal herbs used in the past, it is recently applied to foods and used a lot as a health food material.

Ginseng usually takes more than 4 years to grow, and to get the highest quality ginseng, it has to be grown for 6 years, which takes a long time, and during harvesting, the yield is higher than the initial cultivation for various reasons such as various pests and natural disasters. There is a disadvantage that the price is expensive, so much time is required to obtain ginseng.

Ginseng has roots and brains under the ground and consists of stems, leaves, and fruits on the ground. More specifically, the roots and brains of the roots, roots, and roots of the subterranean ground, stems connected from the brainstem, leaves extending from the stems, fruits (stalks, fruits) at the end of the stems, and small parts connecting the stems and the fruits. There is a vase which is a branch (see FIG. 1).

Ginseng begins to bloom in ginseng for more than three years, and green fruits appear in May. By mid-July, the green fruits of ginseng will ripen and turn red.

Ginseng cultivation farmers use the roots of ginseng, so the ginseng fruit is being picked up when the ginseng fruit begins to promote root growth rather than reproductive growth. At this time, a part of the flower is cut and the flower and ginseng are picked up together.

Ginsenoside means saponin in ginseng, and ginseng contains various kinds of ginsenosides. Ginsenosides of ginseng include panaxadiol (PD), panaxatriol (PT), and oleane (Oleanane). In addition to the ginsenoside component, ginseng includes carbohydrates such as starch, antioxidant aromatic compounds of polyacetylene, gomisin (Gomisin N-A) for protecting the liver, and acidic peptides having a similar insulin action.

The main pharmacological action of ginsenosides, including the central nervous system, affects the endocrine system, the immune system, the metabolic system, and has various effects on body control functions. In addition, ginsenosides (1) have a large lipolytic ability, promote nutrient absorption and digestion, (2) promote metabolism by activating enzymes in cells, and (3) increase energy to restore energy, fatigue, and helplessness. It is known to have an effect on improving anorexia, and (4) promoting the synthesis of serum proteins.

According to the present invention, much of ginsenoside Re is known to have central nervous system inhibitory activity, DNA and RNA promoting action, prasmin activating action, adrenal cortex stimulating hormone secretion, and Rd promotes corticosteroid secretion. , Rb2 is known to have a central nervous system inhibitory action, DNA and RNA synthesis promoting action, plasmin activation action, adrenal cortical stimulating hormone secretion action, antidiabetic action.

However, the ginseng fruit and ginseng vase are removed after being removed from ginseng for growth of ginseng roots, and thus the use of ginsenoside contained in ginseng fruit and ginseng vase is insufficient.

An object of the present invention is to provide a method for preparing ginseng fruit and ginseng vase with high content of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re and other ginsenosides. .

Ginseng fruit and ginseng vase by the present invention has a high content of ginsenosides can be provided by applying them to health supplements can provide a health supplement foods with a high content of ginsenosides.

The present invention steamed and dried the light-treated ginseng fruit and ginseng vase with steam at least one time and then immersed the steamed and dried ginseng fruit and ginseng vase in a solution containing ginsenoside decomposed to a high content of ginsenosides. To provide a method of manufacturing ginseng fruit and ginseng vase.

The ginseng fruit and ginseng vase with much higher content of ginsenosides prepared by the method of the present invention, as in the results of the test example, are processed using the ginseng fruit and ginseng vase which are simply discarded in conventional ginseng farms. Can be obtained.

In addition, the ginseng fruit and the ginseng vase of the present invention are concentrated with a concentrator to obtain a concentrate of ginseng fruit and ginseng vase with high content of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, and ginsenoside Re. Can be.

In accordance with the present invention, ginseng fruits and ginseng vases having excellent content of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re and other ingredients can be obtained from ginseng fruit. Therefore, it can contribute to the income increase of ginseng cultivated farmers.

The present invention comprises the steps of steaming and drying the light-treated ginseng fruit and ginseng vase with steam at least one time, and immersing the steamed and dried ginseng fruit and ginseng vase in a solution containing ginsenoside decomposition products. It shows the manufacturing method of ginseng fruit and ginseng vase with high ginsenoside content.

Hereinafter, the present invention will be described in more detail by each step.

1. Steaming and drying after light irradiation of ginseng fruit and ginseng vase

In the present invention, in order to increase the content of ginsenosides in ginseng fruit and ginseng vase, ginseng fruit and ginseng vase are preferably treated by irradiating light after obtaining from ginseng.

The light may be treated by irradiating sunlight containing ultraviolet rays, visible rays, and infrared rays.

In the above light, any one or more selected from ultraviolet rays, visible rays, and infrared rays may be used.

In the above, the ultraviolet ray may be an ultraviolet ray having a wavelength band of 10 to 397 nm.

As the visible light, visible light having a wavelength range of 380 to 770 nm may be used.

In the infrared, infrared rays having a wavelength range of 0.75 μm to 1000 μm may be used.

Irradiation of light on the ginseng fruit and the ginseng vase can be carried out for 1 to 24 hours. If the irradiation time of ginseng fruit and ginseng vase is less than 1 hour, the increase of ginsenoside content of ginseng fruit and ginseng vase is insignificant, and if it exceeds 24 hours, the effect on the increase of ginsenoside content of ginseng fruit and ginseng vase Rather, there is a fear that ginseng fruit and other nutrients contained in ginseng vase will decrease. Therefore, when the ginseng fruit and ginseng vase with a high content of ginsenosides of the present invention, the light irradiation treatment of the ginseng fruit and the ginseng vase is preferably performed for 1 to 24 hours.

Ginseng fruit and ginseng vase in order to increase the content of ginsenosides or to form various kinds of ginsenoside in ginseng fruit and ginseng vase when ginseng fruit and ginseng vase with high content of ginsenosides of the present invention can do.

As an example of steaming ginseng fruit and ginseng vase in the present invention, it can be performed several times for 1 to 3 hours with steam at 90 to 120 ° C.

Steaming of the ginseng fruit and ginseng vase is preferably performed at least once.

Steaming of the ginseng fruit and the ginseng vase can be performed four times for 1 to 3 hours with steam at 90 to 120 ℃.

Drying is carried out to remove steam from steamed ginseng fruit and ginseng vase.

When the ginseng fruit and the ginseng vase are steamed several times with steam, the steaming and drying may be performed immediately after one steaming is finished.

The steaming and drying of the ginseng fruit and the ginseng vase can be carried out at a temperature of 65 to 75 ℃ to remove the water vapor outside the ginseng fruit and the ginseng vase.

The ginseng fruit and the ginseng vase can be dried by steaming ginseng fruit and ginseng vase using a conventional method. As one example of the drying method, any one or more selected from natural drying at room temperature, hot air drying using hot air, and freeze drying at low temperature may be used. Preferably, the ginseng fruit and the ginseng vase can be dried to the extent that the ginseng fruit and the water vapor outside the ginseng vase can be removed at a temperature of 65 to 75 ° C.

2. Dip steamed and dried ginseng fruit into solution containing ginsenoside degradant

Ginseng contains various kinds of ginsenosides. These ginsenosides are not directly absorbed by the human body, but are known to be absorbed by the ginsenoside degrading products such as enzymes in the body and then absorbed by the ginsenosides. have.

Therefore, the present invention is the ginsenoside and / or ginsenoside component content that is easily absorbed by the human body by immersing the ginseng fruit and ginseng vase with ginsenoside decomposition products and steamed and dried with steam treatment, steamed and steamed in the above solution Many of the ginseng fruit and ginseng vase can provide a manufacturing method.

In the above ginsenoside decomposed product, any one or more selected from Hasegawa bacteria, alpha-glucosidase, alpha-rhamnosidase, and soya safogenol B may be used.

The solution containing the ginsenoside decomposition product may be obtained by adding 1 to 20% of the ginsenoside decomposition product to the purified water by weight.

In the solution containing ginsenoside decomposition products, when the ginsenoside decomposition products are added in an amount less than 1% by weight of purified water, the content of ginsenosides and / or ginsenoside components which are easily absorbed by the human body does not increase, but exceeds 20%. When added, there is no obvious effect of increasing the content of ginsenosides and / or the increase in the content of ginsenosides, which are easily absorbed by the human body, and in addition to ginsenosides, the ginseng fruit and other components contained in ginseng vases are affected. It is not preferable because it gives. Therefore, in the present invention, it is preferable to irradiate light with a solution in which 1 to 20% of ginsenoside decomposition product is added to the purified water by weight, dipping steamed and dried ginseng fruit and ginseng vase with water vapor.

In the light irradiation treatment, steaming and steaming and dried ginseng fruit and ginseng bottle can be immersed in a solution in which ginsenoside decomposition products are dissolved for 10 minutes to 24 hours.

The present invention further performs any one or more treatments selected from ultrasonic treatment, low frequency treatment, electric field treatment, and magnetic field treatment for ginseng fruit and ginseng vase treated with the first and second stages of ginseng with high content of ginsenoside components. Can produce fruit and ginseng vase.

In the above, the ultrasonic treatment may be performed for 10 minutes or more at 20kHz or more.

The ultrasonic treatment may be performed for 10 minutes to 2 hours at 20 to 50 kHz.

Ginsenosides with high content of ginsenosides of the present invention were subjected to ultrasonic treatment under various conditions in the manufacture of ginseng fruit and ginseng vase, and the ginsenoside component corresponding to the object of the present invention when treating ginseng fruit and ginseng vase under the conditions mentioned above. Many ginseng fruits and ginseng vases can be produced.

The low frequency treatment can be performed for 30 minutes or more at 10 kHz or less.

The low frequency treatment can be performed at 5 to 10 kHz for 30 minutes to 2 hours.

Ginseng fruit containing ginsenoside content of ginseng fruit and ginseng vase of the present invention was subjected to low frequency treatment under various conditions. Ginsenoside components satisfying the object of the present invention when treating ginseng fruit and ginseng vase under the conditions mentioned above Many ginseng fruits and ginseng vases can be produced.

The electric field treatment may be performed for 30 seconds or more in an electric field of 5kv or more.

In the above, the electric field treatment can be carried out at 5 to 20kv for 30 seconds to 5 minutes.

Ginseng fruit and ginseng vase with high content of ginsenosides of the present invention were subjected to electric field treatment under various conditions. The ginsenoside component corresponding to the object of the present invention when treating ginseng fruit and ginseng vase under the above-mentioned conditions Many ginseng fruits and ginseng vases can be produced.

The magnetic field treatment may be performed for 30 minutes or more in a magnetic field of 5 Gauss or more.

The above magnetic field treatment can be carried out at 5 to 30 gauss for 30 minutes to 1 hour.

Ginsenosides with high ginsenoside content of the present invention were subjected to magnetic field treatment under various conditions in the manufacture of ginseng fruit and ginseng vase, and the ginsenoside component corresponding to the object of the present invention when treating ginseng fruit and ginseng vase under the conditions mentioned above. Many ginseng fruits and ginseng vases can be produced.

The present invention includes ginseng fruit prepared by the above-mentioned method.

The present invention includes the ginseng vase prepared by the above-mentioned method.

The present invention includes a health supplement comprising a ginseng fruit having a high content of ginsenosides by the above-mentioned method.

The present invention includes a dietary supplement comprising a ginseng vase with high ginsenoside content by the above-mentioned method.

For example, the health supplement may be provided in the form of a liquid beverage and solid tablets, pills, powders, and capsules.

The health supplement of the present invention may contain 0.1 to 10% of ginseng fruit and / or ginseng vase with high content of ginsenosides prepared above as a constituent, based on the total weight of the health supplement, and other excipients and lubricants And submaterials such as lubricants, binders, disintegrants. The subsidiary materials are generally used when provided in the form of solid tablets, pills, powders, and capsules, and are considered to be appropriately selected by those skilled in the art, and detailed descriptions thereof will be omitted.

The ginseng fruit and / or ginseng flower bottle included as a constituent of the health supplement food may be included in any one or more forms selected from powder, juice, hot water extract, and organic solvent extract.

The powder of the ginseng fruit and / or ginseng vase may be one obtained by powdering the ginseng fruit and / or ginseng vase with a grinder.

The juice of the ginseng fruit and / or ginseng vase can be used to extract the juice of ginseng fruit and / or ginseng vase with a juicer.

The hot water extract of the ginseng fruit and / or ginseng vase is placed in 3 to 10 times the purified water relative to the weight of the ginseng fruit and / or ginseng vase and the content of the first purified water at 80 to 120 ° C. The filtrate filtered after extraction to% and / or the concentrate which concentrated the said filtrate can be used.

The organic solvent extract of the ginseng fruit and / or ginseng vase is placed in 2-5 times the organic solvent of the ginseng fruit and / or ginseng vase to the weight of the ginseng fruit and / or ginseng bottle for 1 hour to 24 hours at 65-75 ℃. The filtrate filtered after extraction and / or the concentrated concentrate of the filtrate can be used. At this time, the organic solvent may be removed from the filtrate by performing the step of removing the organic solvent from the filtrate.

The organic solvent used to obtain the organic solvent extract of the ginseng fruit and / or ginseng vase may be an alcohol solvent having 1 to 20 carbon atoms.

The organic solvent used to obtain the organic solvent extract of the ginseng fruit and / or ginseng bottle may be any one selected from methanol, ethanol, propanol, butanol, pentanol.

Ethanol may be used as the organic solvent used to obtain the organic solvent extract of the ginseng fruit and / or ginseng bottle.

Hereinafter, the content of the present invention will be described in detail through examples and test examples. However, these are intended to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1-1>

Ginseng fruit containing a high content of ginsenosides was prepared using the following process.

The ginseng fruit collected from ginseng was irradiated with infrared rays having a wavelength of 100 μm for 10 hours.

1 kg of the above-infrared irradiated ginseng fruit was steamed for 1 hour in a steam of 95 ℃ and then dried at a temperature of 70 ℃.

After steaming and drying for four times, 1 kg of ginseng fruit was put in a solution containing ginsenoside decomposed product and soaked for 30 minutes.

The solution containing the ginsenoside decomposition product was obtained by adding alpha-glucosidase to purified water by 10% by weight of purified water as the ginsenoside decomposition product.

<Example 1-2>

A ginseng vase with an increased content of ginsenoside components was prepared in the same manner as in Example 1-1 except that the ginseng vase collected from ginseng was used.

<Example 2-1>

A ginseng fruit was prepared in the same manner as in Example 1-1, except that the ginseng fruit was irradiated with ultraviolet rays having a wavelength of 100 nm for 5 hours.

<Example 2-2>

A ginseng vase with an increased content of ginsenosides was prepared in the same manner as in Example 2-1, except that the ginseng vase collected from ginseng was used.

<Example 3-1>

A ginseng fruit was prepared in the same manner as in Example 1-1, except that the ginseng fruit was irradiated with visible light having a wavelength of 500 nm for 7 hours.

<Example 3-2>

A ginseng vase with increased content of ginsenosides was prepared in the same manner as in Example 3-1, except that the ginseng vase collected from ginseng was used.

<Example 4-1>

The ginseng fruit collected from ginseng was irradiated with infrared rays having a wavelength of 100 μm for 10 hours.

1 kg of the above-infrared irradiated ginseng fruit was steamed for 1 hour in a steam of 95 ℃ and then dried at a temperature of 70 ℃.

After steaming and drying for four times, 1 kg of ginseng fruit was put in a solution containing ginsenoside decomposed product and soaked for 30 minutes.

1 kg of ginseng fruit immersed in the immersion treatment was carried out at 40 kHz for 1 hour and subjected to ultrasonic treatment.

The solution containing the ginsenoside decomposition product was obtained by adding alpha-glucosidase to purified water by 10% by weight of purified water as the ginsenoside decomposition product.

<Example 4-2>

A ginseng vase with increased content of ginsenosides was prepared in the same manner as in Example 4-1, except that the ginseng vase collected from ginseng was used.

<Example 5-1>

Ginseng fruit was prepared in the same manner as in Example 4-1, except that the ginseng fruit was subjected to low frequency treatment at 7 kHz for 1 hour instead of ultrasonication.

<Example 5-2>

A ginseng vase with an increased content of ginsenoside components was prepared in the same manner as in Example 5-1, except that the ginseng vase collected from ginseng was used.

<Example 6-1>

Ginseng fruit was prepared in the same manner as in Example 4-1, except that the ginseng fruit was subjected to electric field treatment for 3 minutes at 15 kv instead of ultrasonication.

<Example 6-2>

A ginseng vase with an increased content of ginsenosides was prepared in the same manner as in Example 6-1, except that the ginseng vase collected from ginseng was used.

<Example 7-1>

Ginseng fruit was prepared in the same manner as in Example 4-1, except that the ginseng fruit was subjected to magnetic field treatment at 20 gauss for 40 minutes instead of ultrasonication.

<Example 7-2>

A ginseng vase with an increased content of ginsenoside components was prepared in the same manner as in Example 7-1 except that the ginseng vase collected from ginseng was used.

<Test Example 1>

Ginseng fruit prepared in Example 1-1, Example 2-1, Example 3-1, Example 4-1, Example 5-1, Example 6-1 and Example 7-1 The ginseng extract obtained by hot water extraction until the content of purified water became 30% at 100 ° C. was used as a test zone.

The ginseng root sold as a commodity in the market was the same as the hot water extraction conditions of the experimental zone, and the ginseng extract obtained by hot water extraction was used as a control.

The ginsenoside content of the ginseng extract of each experimental and control group obtained above was measured and the results are shown in Table 1 below.

Table 1. Ginsenoside content of ginseng extract of Examples and Comparative Examples

Item Ginsenoside content Example 1-1 105.4 Example 2-1 105.2 Example 3-1 105.5 Example 4-1 107.6 Example 5-1 107.5 Example 6-1 107.4 Example 7-1 107.6 Control 100

The ginsenoside content of Table 1 was measured using ginsenoside Rd as a standard, and the sample was separated into water and butanol, and the butanol layer was dried. The dried sample and the standard were dissolved in glacial acetic acid, and 15% antimony trichloride solution It is the value which measured after absorbance at 525 nm by cooling after heating for 30 minutes at 60 degreeC. At this time, when the ginsenoside content of the control was set to 100, the ginsenoside content of each example indicates the ratio to the content of the control, and the higher the value, the higher the ginsenoside content.

<Test Example 2>

The ginseng vase prepared in Examples 1-2, Example 2-2, Example 3-2, Example 4-2, Example 5-2, Example 6-2 and Example 7-2 The extract obtained by hot water extraction was added to 5 times purified water to weight by weight until the content of purified water became 25% at 100 ° C.

The ginseng vase obtained during the ginseng cultivation was subjected to the hot water extraction under the same conditions as the hot water extraction conditions of the experimental zone, and the extract obtained as the control.

The ginsenoside content of the extracts of each of the experimental and control groups obtained above was measured in the same manner as in Test Example 1, and the results are shown in Table 2 below.

Table 2. Ginsenosides content of extracts of Examples and Comparative Examples

Item Ginsenoside content Example 1-2 104.2 Example 2-2 104.1 Example 3-3 104.3 Example 4-2 106.4 Example 5-2 106.3 Example 6-2 106.2 Example 7-2 106.4 Control 100

<Test Example 3>

The ginseng fruit prepared in Example 1-1 and the Ginseng fruit prepared in Example 1-1 and the Ginsenoside content of black ginseng, black ginseng, red ginseng, and red ginseng obtained in the Republic of Korea were analyzed. The results are shown in Table 3 below.

Table 3. Results of ginsenoside component analysis on ginseng fruit (mg / g)

Item Example 1-1 Black root Black ginseng Red ginseng root Red ginseng Ginsenoside Rg3 4.51 9.18 14.23 0.18 0.10 Ginsenoside Rb1 12.98 1.78 4.78 0.69 1.37 Ginsenoside Rb2 23.11 1.51 3.99 0.19 0.71 Ginsenoside Rc 14.74 1.32 3.93 0.25 0.12 Ginsenoside Rd 31.05 1.14 5.68 0.26 0.10 Ginsenoside Re 135.16 0.20 1.40 0.40 0.99 Ginsenoside Rf - 0.68 1.00 0.22 0.31 Ginsenoside Rg2 3.01 0.28 0.54 0.18 0.22 Ginsenoside Rh1 2.22 1.06 1.17 Confirm Confirm Ginsenoside Rh2 0.04 - - - - Ginsenoside X 0.52 - - - -

-: Not detected

As shown in the results of Table 3, ginsenoside component content contained in the ginseng fruit prepared in Example 1-1 of the present invention and ginsenosides contained in black ginseng, black ginseng, red ginseng root, and red ginseng, respectively, obtained in Korean Patent No. 0543862. In comparison with the side component content, it can be seen that the ginseng fruit prepared by the present invention has a particularly high content of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, and ginsenoside Re. .

As described above, the present invention has been described with reference to the preferred embodiments and test examples, but a person skilled in the art may vary the present invention without departing from the spirit and scope of the present invention as set forth in the claims below. It will be understood that modifications and changes can be made.

Ginseng fruit and ginseng vase with a high ginsenoside content according to the present invention can obtain a ginseng fruit and a ginseng vase with a lot of saponim content in addition to the conventional ginseng root, the ginseng fruit with a ginsenoside content and ginseng vase itself or the Ginsenoside content may contribute to the improvement of income of ginseng cultivated farmers by processed products obtained from ginseng fruit and ginseng vase.

1 is a diagram showing the composition of the fruit, leaves, stems, roots of ginseng.

Claims (4)

Steaming and drying the light-treated ginseng fruit and ginseng vase with steam at least once, Method of producing a ginseng fruit and ginseng vase with high ginsenoside content comprising the step of immersing the steamed and dried ginseng fruit and ginseng vase in a solution containing ginsenoside decomposition products. The method according to claim 1, wherein the light is at least one selected from infrared rays, visible rays, and ultraviolet rays. According to claim 1, steamed and dried ginseng fruit and ginseng vase is any one or more selected from Hasegawa bacteria, alpha-glucosidase, alpha-Rhamnosidase, Soya Safogenol B A method for producing ginseng fruit and ginseng vase with high ginsenoside content, which is immersed in a solution containing the senoside decomposition product. The method according to claim 1, wherein the ginseng fruit and the ginseng vase are immersed in a solution containing the ginsenoside decomposition product, and then the ginseng fruit and the ginseng bottle are added to at least one treatment step selected from ultrasonic treatment, low frequency treatment, electric field treatment, and magnetic field treatment. Ginsenoside content of ginseng fruit and ginseng vase, characterized in that it is further carried out.

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