TW200602487A - Polypeptides having DNA polymerase activity - Google Patents

Polypeptides having DNA polymerase activity

Info

Publication number
TW200602487A
TW200602487A TW094118437A TW94118437A TW200602487A TW 200602487 A TW200602487 A TW 200602487A TW 094118437 A TW094118437 A TW 094118437A TW 94118437 A TW94118437 A TW 94118437A TW 200602487 A TW200602487 A TW 200602487A
Authority
TW
Taiwan
Prior art keywords
dna polymerase
polymerase activity
polypeptides
polypeptide
amplifying
Prior art date
Application number
TW094118437A
Other languages
English (en)
Inventor
Yoshimi Sato
Kazue Nishiwaki
Nana Shimada
Shigekazu Hokazono
Takashi Uemori
Hiroyuki Mukai
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Publication of TW200602487A publication Critical patent/TW200602487A/zh

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1081Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
TW094118437A 2004-06-04 2005-06-03 Polypeptides having DNA polymerase activity TW200602487A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2004166541 2004-06-04

Publications (1)

Publication Number Publication Date
TW200602487A true TW200602487A (en) 2006-01-16

Family

ID=35462914

Family Applications (1)

Application Number Title Priority Date Filing Date
TW094118437A TW200602487A (en) 2004-06-04 2005-06-03 Polypeptides having DNA polymerase activity

Country Status (8)

Country Link
US (3) US7704713B2 (zh)
EP (3) EP1757698B1 (zh)
JP (1) JP4685768B2 (zh)
KR (3) KR20070032737A (zh)
CN (1) CN1993468B (zh)
CA (1) CA2568998A1 (zh)
TW (1) TW200602487A (zh)
WO (1) WO2005118815A1 (zh)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110020877A1 (en) * 2008-01-11 2011-01-27 Genesys Limited Cren7 chimeric protein
GB0803628D0 (en) * 2008-02-28 2008-04-02 Genesys Ltd Enzyme
GB0804722D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
GB0804721D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
JP5977000B2 (ja) * 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
JP6478445B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性dnaポリメラーゼ
CN104250641B (zh) * 2013-06-28 2017-09-01 北京福安华生物科技有限公司 一种高保真dna聚合酶及其制备和应用
GB201802744D0 (en) * 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
JP7563679B2 (ja) 2019-02-28 2024-10-08 池田食研株式会社 遺伝子増幅法及び遺伝子増幅用キット
WO2024138419A1 (zh) * 2022-12-28 2024-07-04 深圳华大生命科学研究院 具有dna聚合酶活性的多肽及其应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3909710A1 (de) 1989-03-23 1990-09-27 Boehringer Mannheim Gmbh Verfahren zur expression eines rekombinanten gens
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US5948663A (en) 1990-12-03 1999-09-07 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5866395A (en) 1990-12-03 1999-02-02 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
JP3132624B2 (ja) 1994-05-09 2001-02-05 東洋紡績株式会社 超好熱始原菌由来のdnaポリメラーゼ遺伝子およびその用途
JP3112148B2 (ja) 1995-05-31 2000-11-27 東洋紡績株式会社 核酸の増幅方法およびその試薬
US6033859A (en) 1996-05-24 2000-03-07 Toyo Boseki Kabushiki Kaisha Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1
JP3487394B2 (ja) * 1996-07-29 2004-01-19 東洋紡績株式会社 改変された耐熱性dnaポリメラーゼおよびその用途
DE69725076T2 (de) 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
EP0834571A1 (en) 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostable nucleic acid polymerase from Thermococcus gorgonarius
EP0834570A1 (en) 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostable nucleic acid polymerase from Thermococcus gorgonarius
US6183998B1 (en) * 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US6107038A (en) * 1998-08-14 2000-08-22 Agilent Technologies Inc. Method of binding a plurality of chemicals on a substrate by electrophoretic self-assembly
US8268605B2 (en) * 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
WO2001032887A1 (en) 1999-10-29 2001-05-10 Stratagene Compositions and methods utilizing dna polymerases
EP1154017B1 (en) * 2000-05-11 2010-01-20 Toyo Boseki Kabushiki Kaisha Modified thermostable dna polymerase from pyrococcus kodakarensis
JP3891330B2 (ja) * 2000-05-11 2007-03-14 東洋紡績株式会社 改変された耐熱性dnaポリメラーゼ
DE60231901D1 (de) * 2001-11-14 2009-05-20 Toyo Boseki Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
DE602004025801D1 (de) * 2003-03-25 2010-04-15 Stratagene California Dna-polymerasefusionen und deren verwendungen

Also Published As

Publication number Publication date
US7704713B2 (en) 2010-04-27
JPWO2005118815A1 (ja) 2008-04-03
EP1757698A4 (en) 2007-08-29
US8344105B2 (en) 2013-01-01
KR20090132644A (ko) 2009-12-30
KR20090132645A (ko) 2009-12-30
EP2292766A2 (en) 2011-03-09
US8048987B2 (en) 2011-11-01
CN1993468B (zh) 2011-04-20
JP4685768B2 (ja) 2011-05-18
EP1757698B1 (en) 2012-05-23
EP2292766A3 (en) 2011-12-07
WO2005118815A1 (ja) 2005-12-15
CN1993468A (zh) 2007-07-04
EP2292767A3 (en) 2011-12-07
US20120083590A1 (en) 2012-04-05
EP2292767A2 (en) 2011-03-09
KR20070032737A (ko) 2007-03-22
EP2292767B1 (en) 2013-11-20
EP2292766B1 (en) 2013-11-20
EP1757698A1 (en) 2007-02-28
CA2568998A1 (en) 2005-12-15
US20100143979A1 (en) 2010-06-10
US20090098610A1 (en) 2009-04-16

Similar Documents

Publication Publication Date Title
TW200602487A (en) Polypeptides having DNA polymerase activity
ATE527381T1 (de) In-vitro-evolution bei mikrofluidischen systemen
WO2007040592A8 (en) Making nucleic acid sequences in parallel and use
NO20072722L (no) Preparater og fremgangsmate for a rense nukleinsyrer fra stabiliseringsreagenser
US7846694B2 (en) Process for producing template DNA and process for producing protein in cell-free protein synthesis system with the use of the same
WO2010051049A3 (en) Thermophilic and thermoacidophilic glycosylation genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
DE60107556D1 (de) Gene für denitrifizierende enzyme
WO2007057167A3 (en) A method for enhancing enzymatic dna polymerase reactions
WO2008051530A3 (en) Polymerase enzymes and reagents for enhanced nucleic acid sequencing
WO2001070816A3 (en) Ecdysone receptor-based inducible gene expression system
DE602007012858D1 (de) Verfahren zur durchführung homologer rekombinationen in pflanzen
GB2439039A (en) Identification of a sterol acyltransferase gene
NO20062695L (no) Genvarianter kodende for proteiner fra metabolsk reaksjonsveien til finkjemikalier
WO2005118608A3 (en) 2’-nitrobenzyl-modified ribonucleotides
WO2006030455A8 (en) Dna polymerases having strand displacement activity
WO2006074288A3 (en) Polypeptides having nucleic acid binding activity
WO2006021453A3 (en) Optimized nucleotide sequences encoding sgp 130
ATE541056T1 (de) Verwendung von dna-polymerasen wie exoribonukleasen
DE602006010990D1 (de) Verfahren zur dipeptidherstellung
ATE553194T1 (de) Verfahren zur dipeptidherstellung
DK1639119T3 (da) Fremgangsmåde til mikrobiologisk isomerisering af alpha-hydroxycarboxylsyrer
DE602004029875D1 (de) Zuführung von nukleinsäuren in eukaryontische genome unter verwendung in vitro zusammengesetzter mu-transpositionskomplexe
TW200621984A (en) A method for promoting growth of gene recombinant cell and enhancing production of target gene product
IL195194A0 (en) Thermostable dna polymerase of the archaeal ampullavirus abv and its applications
ZA200404743B (en) Expression vectors and promoters for heterologous gene expression.