2〇〇52®c 九、發明說明： 【發明所屬之技術領域】 本發明疋有關於一種以大果苔每（cranberry，２００２®c IX. Description of the invention: [Technical field to which the invention belongs] The present invention relates to a kind of cranberry,
Vaccinium macrocarpon Ait·)萃取物作為有效成分的口服 用皮膚改善劑。 【先前技術】Vaccinium macrocarpon Ait ·) extract is used as an active ingredient for improving skin. [Prior art]
大果苔苺生長在北美洲等寒帶地區，為杜鵑花科、烏 飯樹屬（Vaccinium)、蔓狀苔苺(0xycoccus)節的一種植物， 所結的果實為小果實。在美國進行的品種改良，實施大規 f的栽種。大果苔苺的果實可直接食用，在本發明出現之 前’就被廣泛地，可以作為水果乾，還可用以作為果 汁等飲料、料理用調味料、果f、果料的原料使用。此 夕日卜’眾所皆知，攝取大果¥苺果汁，對於治療尿道傳染病 疋有效的（參考 j· Am· Med· Association，271 ·· 751-754 女：^以ΐ，使用大果苔苺的化妝品的例子，主要是由 r判二的壓榨過遽物及/或萃取物所組成的皮膚緊 主:，而大果苔苺果實的壓榨過濾、物及/或萃取物 3=6t(r?eyanin)聚合體所組成的(參考日本專利特 然而’上述所提及的全部較“外關mt)途 5 200528033 16195pif.doc 方面目前並不清楚。 【發明内容】 本發明的目的就是在提供一種以大果苔苺萃取物作 為有效成分的口服用皮膚改善劑。 ，發明人為解決上述問題，積極研究的結果發現，以 大^苔苺萃取物作為口服用皮膚改善劑是有用的，而完成 本發明。意即，本發明下述的口服用皮膚改善劑。 (1) 一種口服用皮膚改善劑，其特徵在於以大果苔苺萃 取物作為有效成分。 口 (2) 上述(1)中所述之口服用皮膚改善劑，其具有皮膚保 溼、調整皮脂量、保持皮膚彈性或保持末梢血液循環的任 何一項以上機能。 (3) 種食或藥品，其特徵在於含有（1)或(2)所述之 口服用皮膚改善劑。 為讓本發明之上述和其他目的、特徵和優點能更明顯 易懂，下文特舉較佳實施例，並配合所附圖式，作詳細說 明如下。 【實施方式】 本發明提出一種以大果苔苺萃取物作為有效成分的 口服用皮膚改善劑。 有效成份是大果苔莓萃取物，例如是依照常法由大果 苔莓榨汁所得到的果汁或果汁的濃縮物、在果汁或果汁的 濃縮物中添加並混合葡聚醣等賦形劑，再利用噴霧乾燥或 冷凍乾燥等方法所得到之大果苔莓果汁中的固體成份、或 6 200528〇33 是使用適當的溶劑從大果苔莓 里士 ―、您工一 ,,^ ^ ^ 不口每的果貝、果皮或種子卒取出 、卞_、 迟的大果苔莓萃取物經口服攝取，能笋揮 改善皮膚的作。 ㈣㈣月4揮 ^發：戶!得到的大果苔苺萃取物的有效成份，舉例來 况，例如疋原花青素（proanthocyanidin)、花青素 (anthocyanm)、黃酮配糖體（flabv_咖⑽_等 (，~。_、金雞鈉酸(quinic娜）、捧樣酸(dtric 荨有機酸類、無機物及維仙人楚 ^It is a plant in the azalea family, genus Vaccinium, and 0xycoccus node, and its fruit is small fruit. Variety improvement in the United States, implementation of large-scale f planting. The fruit of the big fruit moss can be eaten directly. Before the invention, it was widely used as dried fruit and as a raw material for drinks such as fruit juice, cooking seasoning, fruit f, and fruit material. It is well known today that ingesting big fruit ¥ berry juice is effective for treating urinary tract infectious diseases (see j · Am · Med · Association, 271 ·· 751-754 Female: ^ Yi, using large fruit moss An example of a berry cosmetic is mainly a skin compact consisting of a pressed extract and / or extract of r2: and a squeeze filter, extract and / or extract of the fruit fruit berry 3 = 6t ( r? eyanin) polymer (refer to Japanese patent, but 'all of the above mentioned are more "foreign relations mt." 5 200528033 16195pif.doc is currently unclear. [Abstract] The purpose of the present invention is to Provided is an oral skin improving agent which uses a large fruit berry extract as an active ingredient. In order to solve the above problems, the inventors have found that active research has found that it is useful to use the large fruit berry extract as an oral skin improvement agent, and The present invention has been completed. In other words, the following oral skin improving agent of the present invention is provided. (1) An oral skin improving agent, which is characterized in that a large fruit berry extract is used as an active ingredient. Mouth (2) The above (1) Oral skin An improving agent, which has any one or more functions of moisturizing the skin, adjusting the amount of sebum, maintaining skin elasticity, or maintaining peripheral blood circulation. (3) A food or a medicine, characterized in that it contains one of (1) or (2) Oral skin improving agent. In order to make the above and other objects, features, and advantages of the present invention more comprehensible, the preferred embodiments are described below in conjunction with the accompanying drawings, and described in detail as follows. The invention proposes an oral skin improving agent that uses a large fruit berry extract as an active ingredient. The effective ingredient is a large fruit berry extract, for example, a fruit juice or fruit juice concentrate obtained from a large fruit berry extract according to a conventional method. Solids in fruit juice or fruit juice concentrates by adding and mixing excipients such as dextran and spray drying or freeze drying, or 6 200528〇33 is used Appropriate solvents are taken from the large fruit moss raspberry, ^ ^ ^ ^ ^ ^ ^ shells, peels or seeds are not taken out, 卞 ,, late fruit berry extract can be taken orally, can shoot Improve 4 月 4 月 挥发: The active ingredients of the large fruit moss raspberry extract obtained by the household, for example, such as proanthocyanidin, anthocyanm, and flavv_カ ⑽_ 等 (, ~ ._, quinic sodium, quinic acid, dtric net organic acids, inorganic substances and Weixianrenchu ^
肩物及、准他命專。其中，較佳的情況是原 化月，、化月’、等夕紛類、金雞納酸等有機酸類的含量越 多越好。 在進行萃取處理中，所使用的溶劑具有特別的限定， 例如是使用-般的極性溶劑或雙極性溶劑。其中，溶劑例 如是使用水、有機溶劑、含水或有機溶_溶劑或水和 機溶劑的混合溶液。其巾，有機溶劑例如是低級醇(具#來 說如乙醇(ethanol)、甲醇(methan〇1)、丙醇(pr〇pan〇l)、、^ 醇 (butanol))、乙醚（ether)類（具體來說如二乙醚 ether))、鹵化烴（hal〇gen hydrocarbon)(具體來說如氣仿 (chloroform))、亞硝酸鹽（nitrne)類（具體來說如乙氰 (acetonitrile))、酯（ester)類（具體來說如乙酸乙酯 acetate))、S同(ketone)類(具體來說如丙酮(acetone))等。在使 用性方面以乙醇、甲醇或乙酸乙酯較佳。此外，有機溶劑 以兩種以上混合使用為佳。 ’片 另外，為了濃縮/精製大果苔苺萃取物的有效成份，還 可以進行溶劑萃取，及以合成吸附劑與離子交換樹脂進行 7 200528033 處理。 如實施例所示，因為大果苔苺萃取物具有皮膚保渔、 调整皮脂量、保持皮膚彈性或保持末梢血液楯環的機能， 所以可作為口服用皮膚改善劑使用。 月匕 皮膚保濕，是指能良好地保持皮膚中水分含量，以捭 進皮膚摸起來的光滑感、滑溜感或濕潤感等。里 曰 調整皮脂量，是指能良好地調整皮脂量，以 油、粉刺或面皰等情況。 °〆Shoulder and quasi-death. Among them, a better case is the original month, the more the content of organic acids such as ceratoic acid, crotonic acid, etc., the better. In performing the extraction treatment, the solvent used is particularly limited, for example, a polar solvent or a bipolar solvent is used. Among them, the solvent is, for example, water, an organic solvent, an aqueous or organic solvent, or a mixed solution of water and an organic solvent. The organic solvent is, for example, a lower alcohol (for example, ethanol, methanol, methan〇1, propanol, butanol), or ether. (Specifically, such as diethyl ether), halogen hydrocarbons (specifically, such as chloroform), nitrites (specifically, such as acetonitrile), Ester (specifically, such as ethyl acetate), S ketone (specifically, such as acetone), and the like. In terms of usability, ethanol, methanol or ethyl acetate is preferred. The organic solvent is preferably used in combination of two or more. In addition, in order to concentrate / refine the effective components of the berry extract, you can also perform solvent extraction and a synthetic adsorbent and ion exchange resin treatment. As shown in the examples, the berry extract has the functions of preserving the skin, adjusting the amount of sebum, maintaining the elasticity of the skin, or retaining the peripheral blood loops, so it can be used as an oral skin improver. Moon Dagger Skin moisturization refers to the ability to keep the moisture content in the skin well, and to feel the smoothness, slippery feeling, or moisturization of the skin. Here, adjusting the amount of sebum means that the amount of sebum can be well adjusted, such as oil, acne or facial blister. ° 〆
保持皮膚彈性’是指良好地保持皮膚彈性，以 膚的張力或改善眼下皮膚鬆弛等情況。 曰皮 ，持末梢，液循環’是指良好地保持末梢血液循環， 以改善血液循環差的情況。 =發_劑’單就财身或是添加 中，可以液態或固態的方式使 R次条ασ 話，劑的形狀特別限定在錠要作為口服使用的 液_。有效成份121顆粒劑、散劑或 程度、用藥目的、使用者的縮跟精製的 舉例來說，口服使用的h Λ狀心而作出適當的設定。 天服用o._lg〜50g/K;為f照使用者的體重而定，每 〇.〇〇〇5g^25g/Kg，^ ^ ^ 本發明的口服田忐忐勹却人服用O.OOlg〜l〇g/Kg。 使用的時間至少兩週，以持續性使用為佳，持續 以上。 奴佳的為四週以上，更佳的為八週 以下 根據貫施例所示 描述本發明的大果苔莓萃取 8 200528033 16195pif.doc 物對於皮膚改善作用的確認方法及其確認的結果。此外， 此一實施例並不用以限定本發明。 【實施例】 (口服攝取大果苔莓萃取物的皮膚改善效果） 以同意參加實驗的33〜38歲健康女性24名作為對 象’分為實驗組及對照組等兩組(母組12名）。實驗組每天 早晚各喝一瓶市面上販售的125ml大果苔莓果汁(產品名 —UR65」，夺7口一公司出品）。至於 對照組，不攝取此指定的特別飲料，進行季節變動測量監 測。 實驗期間共四週，於開始時及四週後進行「自己報告 的皮膚狀態及身體情況的變化」、「美容專家做的皮膚診 斷」、「皮膚水分含量及皮膚彈性」及「末梢血液循環機 能」的測定。各種測定的實施是於洗臉後，在將環境調整 在溫度23°C與溼度50%RH的環境實驗室中，於座位上^ 息20分鐘後進行。 ® 自己報告的皮膚狀態及身體情況的變化，是以問卷調 查的方式進行，並將此一變化數值化。以開始時間作為基 準的四週後，自己評價的皮膚狀態變化如圖丨所示。每12 名的平均值，實驗組與對照組的比較結果，可以看出全部 的評價項目皆有改善的傾向。特別是在「泛油」、「張力、 「粉刺及面皰」、「透明感」及「皮膚的總合評價」的項 目中，被認為具有統計上的顯著差異。 美容專家做的皮膚診斷為視診及觸診，並將此一變化 9 200528033 16195pif.doc 數值化。以開始時間作為基準的四週後，自己評價的皮膚 狀態變化如圖2所示。每12名的平均值，實驗組與對照組 的比較結果，可以看出全部的評價項目皆有改善的趨勢。 特別是在「魚尾紋」、「眼下皮膚鬆弛」、「張力感」、 皮膚的總J評價」的項目中，被認為具有統計上的顯 著差異此θ驗結果指出’實驗組被認為具有口服攝取 的皮膚改善效果。 S膚水分含量’是以商品名為SKIC〇N侧(公司 除製)的電傳導型商品，測定臉頰中央部份角層(h〇my 的水分含量而得之。測定的結果，即為以開始時間作為基 準的四週後？分含量的變化量，實驗組為+6.城，對照組 為-〇¥’實驗組被認為具有良好的皮膚保濕效果。對自 己報告的、「皮膚摸起來的光滑感」的改善、觸診的「光滑 感」及「漁/閏感」之改善結果進行綜合思考，可了解本發 明的口服用皮膚改善劑具有皮膚保濕的機能。 ^ 皮脂量，是使用透過光比率型油脂計對左側眉毛上方 • 的皮脂程度進行測定而得之，對其進行圖形化並進行判 定。判定的結果，即為以開始時間作為基準的四週後的皮 脂量判定，實驗組中，狀況改善的有四名，沒變化的有四 名，惡化的有四名。另一方面，在對照組中，没狀 善的人，沒變化的有7名，惡化的有五名。這個^吉果指 實驗期間中進行季節變動測量監測的結果，對照1組θ : 量狀況惡化，而實驗組的皮脂量狀況則維持不變、。對自月曰 報告的「泛油」、「粉刺及面皰」的改善情^行綜= 200528〇33 考，可了解本發明的口服用皮膚改善劑具有調整皮脂量的 機能。 皮膚彈性，是使用皮膚彈性測定機SEM575(彳 >于/ 今儿工公司製）對右側頰骨上部的皮膚彈性（反彈率）進行 測定而得之。測定的結果，即為以開始時間作為基準的四 週後皮膚彈性的變化量，實驗組為+5·9%，對照組為 -3.9%，貫驗組被認為具有良好地改善皮膚彈性的效果。對 自己報告的「張力」的改善、視診的「眼下皮膚鬆弛」的 改善及觸診的「張力感」之改善結果進行综合思考，可了 解本發明的口服用皮膚改善劑具有保持皮膚彈性的機能。 /末梢血液循環機能，是使用Bc檢查器（加速度脈波測 定系統’ 7 : ν亇…夕工彳7、、公司製)對食指指尖置於感 測器的-部分巾’㈣纽波作為分數錢行败而得之。 測定的結果’即為以開始時間作為基準的四週後末梢血液 循環機能之分數㈣變化f，實驗組為+39，對照組為 _23.9% ’實驗組被認為具有良好地改善皮膚彈性的效果。 這個結果指出，實驗期間中進行季節變動測量監測的結 果’對照組的末梢血液循環機能狀況惡化，而實驗组 1血液循環機能狀況則維持不變。對自己報告的「血液循 善Ϊ果進行综合思考，可了解本發明的口服用 皮膚改善诏具有保持末梢血液猶環的機#。 本發明的口服用皮膚改善劑具有^保 持〜環的機能，在藥= 200528033 I6l95pif.doc 和範圍内，當“些;:=:不之精神 範圍當視後附之巾請專利範圍所界匕本發明之保護 【圖式簡單說明】 考為準。 圖 是依照本發明一實施例所 緣 狀態的測定結果之示意圖。 丁之自已報告的皮膚 圖2是依照本發明一實施例所、纟會示 觸診的測定結果之示意圖。 【主要元件符號說明】 —〇 之美容專家視診及 12Maintaining skin elasticity 'means to maintain the skin elasticity well, to improve the tension of the skin, or to improve the skin sagging under the eyes. "Skin, holding the peripheral, fluid circulation 'means to maintain the peripheral blood circulation well to improve the poor blood circulation. In the case of the agent alone or in addition, the shape of the agent can be changed to ασ in a liquid or solid state. The shape of the agent is particularly limited to the liquid in which the tablet is to be used orally. Active ingredient 121 granules, powders or degrees, purpose of administration, user's shrinkage and refinement For example, the oral application of h Λ shaped heart makes appropriate settings. Take o._lg ~ 50g / K every day; it depends on the weight of the user, every 0.0005g ^ 25g / Kg, ^^^^ Oral tincture of the present invention is taken by people OOlg ~ lOg / Kg. Use for at least two weeks. Continuous use is preferred. Nujia is more than four weeks, and more preferably eight weeks or less. The method for confirming the skin improving effect of the present invention and the results of the confirmation are described in accordance with the examples. In addition, this embodiment is not intended to limit the present invention. [Examples] (Skin-improving effect of oral ingestion of B. macrophylla extract) 24 healthy women aged 33 to 38 who agreed to participate in the experiment were divided into two groups including an experimental group and a control group (12 mother groups) . The experimental group drank a bottle of 125ml large fruit moss berry juice (product name-UR65 "sold by the company in the morning and evening each day. As for the control group, the specified special drink was not ingested, and seasonal fluctuation measurement monitoring was performed. The experiment period was four weeks in total. At the beginning and after four weeks, the changes of "self-reported skin state and physical condition", "skin diagnosis by a beauty expert", "skin moisture content and skin elasticity", and "peripheral blood circulation function" were performed. Determination. Various measurements were carried out after washing the face, in an environmental laboratory adjusted to an environment at a temperature of 23 ° C and a humidity of 50% RH, and performed on the seat for 20 minutes. ® The changes in skin state and physical condition reported by ourselves are carried out by means of questionnaires and the changes are quantified. Four weeks after the start time is used as a reference, the skin state changes evaluated by yourself are shown in Figure 丨. For the average of 12 people, the comparison between the experimental group and the control group shows that all the evaluation items have a tendency to improve. In particular, the items of "pan oil", "tension," acne and facial blister "," transparency ", and" comprehensive evaluation of skin "are considered to have statistically significant differences. The skin diagnosis made by a beauty expert is visual inspection and palpation, and this change is quantified 9 200528033 16195pif.doc. Four weeks after the start time was taken as a reference, the skin state changes evaluated by myself are shown in FIG. 2. For the average of 12 persons, the comparison results between the experimental group and the control group show that all the evaluation items have a tendency to improve. Especially in the items of "crow's feet", "skin under the eyes", "sense of tension", and total J evaluation of the skin, it is considered to have a statistically significant difference. This test result indicates that the 'experimental group is considered to have oral intake Skin improvement effect. S Skin Moisture Content 'is an electrically conductive product with the trade name SKICON side (manufactured by the company). It is obtained by measuring the moisture content of the central corneal layer (h〇my). The result of the measurement is Four weeks after the start time was taken as the benchmark, the amount of change in the content was divided into the experimental group of +6. City and the control group of -0 ¥ '. The experimental group was considered to have a good skin moisturizing effect. Improvement of "smoothness", palpation of "smoothness", and improvement of "fishing / fishing feeling" By comprehensive consideration, we can understand that the oral skin improving agent of the present invention has the function of moisturizing the skin. ^ The amount of sebum is used through The light ratio type fat meter is obtained by measuring the degree of sebum on the left eyebrows, and graphs and judges it. The result of the judgment is the sebum amount determination after four weeks based on the start time. In the experimental group, There were four who improved, four who did not change, and four who deteriorated. On the other hand, in the control group, there were seven people who did not change well, seven who did not change, and five who deteriorated. This ^ Jiguo Refers to the results of seasonal variation measurement monitoring during the experimental period. Control θ: The amount of the condition deteriorated, while the sebum level of the experimental group remained unchanged. For the "pan oil", "acne and facial rash" reported since the month The improvement of the ^^^ = 200528〇33 test, we can understand that the oral skin improver of the present invention has a function to adjust the amount of sebum. Skin elasticity, using the skin elasticity measuring machine SEM575 (彳 > by / Jiner Kogyo company ) The skin elasticity (rebound rate) of the upper part of the right cheekbone was measured. The measurement result is the change in skin elasticity after four weeks based on the start time. The experimental group was + 5.9%, the control group At -3.9%, the test group was considered to have a good effect on improving skin elasticity. The improvement of the "tension" reported by themselves, the improvement of "slackness of the skin under inspection", and the improvement of "tension" of palpation were performed. By comprehensive consideration, it can be understood that the oral skin improving agent of the present invention has a function of maintaining skin elasticity. / Peripheral blood circulation function is using a Bc tester (acceleration pulse wave measurement system '7: (亇 ... 夕 工 彳 7, made by the company) The part of the towel '㈣ Niubo, whose fingertip is placed on the sensor, was defeated as a fraction of money. The result of the measurement' is four weeks based on the start time. The change in the fraction of posterior peripheral blood circulation function, f, is +39 in the experimental group and _23.9% in the control group. The experimental group is considered to have a good effect on improving skin elasticity. This result indicates that the seasonal fluctuation measurement monitoring during the experimental period Results' The peripheral blood circulation function of the control group deteriorated, while the blood circulation function of the experimental group 1 remained unchanged. By comprehensively thinking about the "blood circulation good results" reported by ourselves, we can understand that the oral skin improvement of the present invention has The machine that keeps peripheral blood still ring. # The oral skin improving agent of the present invention has the function of maintaining ~ ring, in the drug = 200528033 I6l95pif.doc and the range, when "some;: =: 不 的 psychological range when viewed The attached towel is subject to the protection of the invention within the scope of the patent. [Schematic description of the drawing] shall prevail. FIG. Is a schematic diagram showing the measurement results of the status according to an embodiment of the present invention. Ding Zhi's self-reported skin FIG. 2 is a schematic diagram showing the results of palpation measurement according to an embodiment of the present invention. [Description of main component symbols] — 〇 Inspection by beauty experts and 12