SU1484094A1 - Method of purification of antibodies raised to thyroid hormones - Google Patents

Method of purification of antibodies raised to thyroid hormones

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Publication number
SU1484094A1
SU1484094A1 SU4221846/14A SU4221846A SU1484094A1 SU 1484094 A1 SU1484094 A1 SU 1484094A1 SU 4221846/14 A SU4221846/14 A SU 4221846/14A SU 4221846 A SU4221846 A SU 4221846A SU 1484094 A1 SU1484094 A1 SU 1484094A1
Authority
SU
USSR - Soviet Union
Prior art keywords
antibodies
purification
antibodies raised
thyroid hormones
antiserum
Prior art date
Application number
SU4221846/14A
Other languages
Russian (ru)
Inventor
М.Н. Ермоленко
Е.С. Пышко
О.В. Свиридов
О.А. Стрельченок
Original Assignee
Институт биоорганической химии АН БССР
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институт биоорганической химии АН БССР filed Critical Институт биоорганической химии АН БССР
Priority to SU4221846/14A priority Critical patent/SU1484094A1/en
Application granted granted Critical
Publication of SU1484094A1 publication Critical patent/SU1484094A1/en

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Abstract

FIELD: medicine. SUBSTANCE: antiserum is incubated with affinity sorbent containing thyroid hormone. Concentration of latter exceeds the maximal binding capacity of antibodies in antiserum by 5-20-times. Then washing is carried out with solution of NaOH at pH 10,0-12,0 following by antibodies desorption with 15- 50% aqueous solution of acetonitrile at pH 2,0-4,0. EFFECT: simplified method, enhanced affinity of antithyroid antibodies. 2 tbl
SU4221846/14A 1987-04-03 1987-04-03 Method of purification of antibodies raised to thyroid hormones SU1484094A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU4221846/14A SU1484094A1 (en) 1987-04-03 1987-04-03 Method of purification of antibodies raised to thyroid hormones

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU4221846/14A SU1484094A1 (en) 1987-04-03 1987-04-03 Method of purification of antibodies raised to thyroid hormones

Publications (1)

Publication Number Publication Date
SU1484094A1 true SU1484094A1 (en) 1994-01-15

Family

ID=60528093

Family Applications (1)

Application Number Title Priority Date Filing Date
SU4221846/14A SU1484094A1 (en) 1987-04-03 1987-04-03 Method of purification of antibodies raised to thyroid hormones

Country Status (1)

Country Link
SU (1) SU1484094A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2485053A1 (en) * 2011-02-03 2012-08-08 Arkray, Inc. Liquid reagent of thyroid hormone-immobilized carrier and use thereof
RU2551237C2 (en) * 2008-10-20 2015-05-20 Эббви Инк Virus inactivation in antibody purification

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2551237C2 (en) * 2008-10-20 2015-05-20 Эббви Инк Virus inactivation in antibody purification
EP2485053A1 (en) * 2011-02-03 2012-08-08 Arkray, Inc. Liquid reagent of thyroid hormone-immobilized carrier and use thereof
CN102628871B (en) * 2011-02-03 2015-03-18 爱科来株式会社 Liquid reagent of thyroid hormone-immobilized carrier and use thereof
US9551723B2 (en) 2011-02-03 2017-01-24 Arkray, Inc. Liquid reagent of thyroid hormone-immobilized carrier and use thereof

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