ES456196A1 - Method for the purification of heparin - Google Patents
Method for the purification of heparinInfo
- Publication number
- ES456196A1 ES456196A1 ES456196A ES456196A ES456196A1 ES 456196 A1 ES456196 A1 ES 456196A1 ES 456196 A ES456196 A ES 456196A ES 456196 A ES456196 A ES 456196A ES 456196 A1 ES456196 A1 ES 456196A1
- Authority
- ES
- Spain
- Prior art keywords
- heparin
- matrix
- fixed
- buffer
- ionic strength
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A method for the preparation of heparin of high specific activity, characterized in that a heparin solution of a concentration of 0 to 8%, dissolved in a buffer having a ph value comprised between 5 and 9.5 and an ionic strength comprised between 0 and 0.8 m, is adsorbed on plasma proteins that fix heparin, fixed in a matrix, constituted by antithrombin iii fixed in a matrix or an inhibitor of inter.-alpha-trypsin fixed in a matrix, of a higher concentration at 0.1 mg/ml gel, and subsequently it is eluted using a buffer with a ph value between 4.5 and 10 and an ionic strength greater than 1 m. (Machine-translation by Google Translate, not legally binding)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE7603040A SE431218B (en) | 1976-03-05 | 1976-03-05 | HEPARIN PURIFICATION PROCEDURE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES456196A1 true ES456196A1 (en) | 1978-07-01 |
Family
ID=20327240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES456196A Expired ES456196A1 (en) | 1976-03-05 | 1977-02-23 | Method for the purification of heparin |
Country Status (21)
| Country | Link |
|---|---|
| JP (1) | JPS52108012A (en) |
| AT (1) | AT356818B (en) |
| AU (1) | AU508430B2 (en) |
| CA (1) | CA1079272A (en) |
| CS (1) | CS196349B2 (en) |
| DE (1) | DE2709500C2 (en) |
| DK (1) | DK155606C (en) |
| ES (1) | ES456196A1 (en) |
| FI (1) | FI62103C (en) |
| FR (1) | FR2342991A1 (en) |
| GB (1) | GB1539332A (en) |
| HU (1) | HU176811B (en) |
| IE (1) | IE44907B1 (en) |
| IL (1) | IL51346A (en) |
| NL (1) | NL186385C (en) |
| NO (1) | NO144388C (en) |
| NZ (1) | NZ183480A (en) |
| PL (1) | PL103049B1 (en) |
| SE (1) | SE431218B (en) |
| SU (1) | SU736860A3 (en) |
| ZA (1) | ZA771267B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE449753B (en) * | 1978-11-06 | 1987-05-18 | Choay Sa | MUCOPOLYSACCARIDE COMPOSITION WITH REGULATORY EFFECTS ON COAGULATION, MEDICINAL CONTAINING ITS SAME AND PROCEDURE FOR PREPARING THEREOF |
| DE3531101A1 (en) * | 1984-08-31 | 1986-03-13 | Nicolás Huberto Olivos Behrens | A NON-THROMBIC HEPARINE AND A METHOD FOR ITS PRODUCTION |
| US9676816B2 (en) * | 2014-01-21 | 2017-06-13 | Board Of Trustees Of The University Of Arkansas | Heparin affinity tag and applications thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2623001A (en) * | 1949-04-07 | 1952-12-23 | Bengt E G V Sylven | Preparing heparin |
| DE1195010B (en) * | 1962-05-12 | 1965-06-16 | Ormonoterapia Richter S P A | Method for purifying heparin by the chromatographic method |
-
1976
- 1976-03-05 SE SE7603040A patent/SE431218B/en not_active IP Right Cessation
-
1977
- 1977-01-27 IL IL51346A patent/IL51346A/en unknown
- 1977-02-14 CA CA271,731A patent/CA1079272A/en not_active Expired
- 1977-02-15 FI FI770491A patent/FI62103C/en not_active IP Right Cessation
- 1977-02-22 NL NLAANVRAGE7701882,A patent/NL186385C/en not_active IP Right Cessation
- 1977-02-23 ES ES456196A patent/ES456196A1/en not_active Expired
- 1977-02-25 JP JP2016477A patent/JPS52108012A/en active Pending
- 1977-03-02 NZ NZ183480A patent/NZ183480A/en unknown
- 1977-03-03 ZA ZA00771267A patent/ZA771267B/en unknown
- 1977-03-03 IE IE469/77A patent/IE44907B1/en not_active IP Right Cessation
- 1977-03-04 AU AU22953/77A patent/AU508430B2/en not_active Expired
- 1977-03-04 AT AT144577A patent/AT356818B/en not_active IP Right Cessation
- 1977-03-04 GB GB9275/77A patent/GB1539332A/en not_active Expired
- 1977-03-04 SU SU772457090A patent/SU736860A3/en active
- 1977-03-04 DE DE2709500A patent/DE2709500C2/en not_active Expired
- 1977-03-04 NO NO770764A patent/NO144388C/en unknown
- 1977-03-04 DK DK096077A patent/DK155606C/en not_active IP Right Cessation
- 1977-03-04 PL PL1977196438A patent/PL103049B1/en unknown
- 1977-03-04 HU HU77KA1485A patent/HU176811B/en unknown
- 1977-03-04 FR FR7706469A patent/FR2342991A1/en active Granted
- 1977-03-07 CS CS771523A patent/CS196349B2/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IE44907B1 (en) | 1982-05-19 |
| FR2342991A1 (en) | 1977-09-30 |
| ATA144577A (en) | 1979-10-15 |
| FI62103C (en) | 1982-11-10 |
| NZ183480A (en) | 1979-08-31 |
| HU176811B (en) | 1981-05-28 |
| SU736860A3 (en) | 1980-05-25 |
| IL51346A0 (en) | 1977-03-31 |
| DE2709500C2 (en) | 1986-09-18 |
| IL51346A (en) | 1979-11-30 |
| DE2709500A1 (en) | 1977-09-08 |
| NL7701882A (en) | 1977-09-07 |
| FI62103B (en) | 1982-07-30 |
| NO144388C (en) | 1981-08-19 |
| SE431218B (en) | 1984-01-23 |
| CS196349B2 (en) | 1980-03-31 |
| ZA771267B (en) | 1978-01-25 |
| AT356818B (en) | 1980-05-27 |
| DK155606C (en) | 1989-09-11 |
| SE7603040L (en) | 1977-09-06 |
| CA1079272A (en) | 1980-06-10 |
| NO144388B (en) | 1981-05-11 |
| NL186385B (en) | 1990-06-18 |
| GB1539332A (en) | 1979-01-31 |
| FR2342991B1 (en) | 1981-10-30 |
| JPS52108012A (en) | 1977-09-10 |
| NL186385C (en) | 1990-11-16 |
| AU508430B2 (en) | 1980-03-20 |
| NO770764L (en) | 1977-09-06 |
| IE44907L (en) | 1977-09-05 |
| FI770491A (en) | 1977-09-06 |
| DK155606B (en) | 1989-04-24 |
| DK96077A (en) | 1977-09-06 |
| AU2295377A (en) | 1978-09-07 |
| PL103049B1 (en) | 1979-05-31 |
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