SK287793B6 - Spôsob stanovenia molekúl nukleových kyselín - Google Patents
Spôsob stanovenia molekúl nukleových kyselín Download PDFInfo
- Publication number
- SK287793B6 SK287793B6 SK1083-2003A SK10832003A SK287793B6 SK 287793 B6 SK287793 B6 SK 287793B6 SK 10832003 A SK10832003 A SK 10832003A SK 287793 B6 SK287793 B6 SK 287793B6
- Authority
- SK
- Slovakia
- Prior art keywords
- probes
- hybridization
- nucleic acid
- microarray
- acid molecules
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 65
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 63
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 55
- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 239000000523 sample Substances 0.000 claims abstract description 114
- 238000009396 hybridization Methods 0.000 claims abstract description 82
- 238000002493 microarray Methods 0.000 claims abstract description 44
- 238000007403 mPCR Methods 0.000 claims abstract description 22
- 238000002844 melting Methods 0.000 claims abstract description 22
- 230000008018 melting Effects 0.000 claims abstract description 22
- 108090000623 proteins and genes Proteins 0.000 claims description 31
- 230000003115 biocidal effect Effects 0.000 claims description 25
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 18
- 239000000835 fiber Substances 0.000 claims description 13
- 229960002685 biotin Drugs 0.000 claims description 12
- 239000011616 biotin Substances 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 12
- 125000003729 nucleotide group Chemical group 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 11
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 10
- 230000003321 amplification Effects 0.000 claims description 9
- 235000020958 biotin Nutrition 0.000 claims description 9
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 9
- 238000000746 purification Methods 0.000 claims description 8
- 239000011324 bead Substances 0.000 claims description 7
- 101150064107 fosB gene Proteins 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- 102100024746 Dihydrofolate reductase Human genes 0.000 claims description 5
- 108010090804 Streptavidin Proteins 0.000 claims description 5
- 229940126575 aminoglycoside Drugs 0.000 claims description 5
- 108020001096 dihydrofolate reductase Proteins 0.000 claims description 5
- 101150016744 ermC gene Proteins 0.000 claims description 5
- 101150037181 vanB gene Proteins 0.000 claims description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 4
- 229930024421 Adenine Natural products 0.000 claims description 4
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 claims description 4
- 108090000204 Dipeptidase 1 Proteins 0.000 claims description 4
- 102000003960 Ligases Human genes 0.000 claims description 4
- 108090000364 Ligases Proteins 0.000 claims description 4
- 101000735344 Lymantria dispar Pheromone-binding protein 2 Proteins 0.000 claims description 4
- 101710099760 Tetracycline resistance protein Proteins 0.000 claims description 4
- 101150047863 aacA-aphD gene Proteins 0.000 claims description 4
- 229960000643 adenine Drugs 0.000 claims description 4
- 101150071231 mecR1 gene Proteins 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 101100206300 Escherichia coli tetC gene Proteins 0.000 claims description 3
- 108091000080 Phosphotransferase Proteins 0.000 claims description 3
- 101150067314 aadA gene Proteins 0.000 claims description 3
- 102000006635 beta-lactamase Human genes 0.000 claims description 3
- 101150031494 blaZ gene Proteins 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 102000020233 phosphotransferase Human genes 0.000 claims description 3
- 101710116957 D-alanyl-D-alanine carboxypeptidase Proteins 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 229920002994 synthetic fiber Polymers 0.000 claims description 2
- 230000037452 priming Effects 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 108020004414 DNA Proteins 0.000 description 50
- 108091034117 Oligonucleotide Proteins 0.000 description 24
- 239000000872 buffer Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 8
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 230000000295 complement effect Effects 0.000 description 7
- 150000003839 salts Chemical class 0.000 description 5
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 241000194032 Enterococcus faecalis Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000027455 binding Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 206010034133 Pathogen resistance Diseases 0.000 description 2
- 239000002262 Schiff base Substances 0.000 description 2
- 150000004753 Schiff bases Chemical class 0.000 description 2
- 241001147693 Staphylococcus sp. Species 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 125000003172 aldehyde group Chemical group 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- 229960003276 erythromycin Drugs 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- ADIHZDIWDRJIOQ-JFGNBEQYSA-N (2s,5r,6r)-6-[[2-(3-chlorobut-2-enylsulfanyl)acetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Chemical compound S1C(C)(C)[C@H](C(O)=O)N2C(=O)[C@@H](NC(=O)CSCC=C(Cl)C)[C@H]21 ADIHZDIWDRJIOQ-JFGNBEQYSA-N 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 241000186249 Corynebacterium sp. Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 102000055027 Protein Methyltransferases Human genes 0.000 description 1
- 108700040121 Protein Methyltransferases Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000607149 Salmonella sp. Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 241000191963 Staphylococcus epidermidis Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 108010059993 Vancomycin Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 150000004683 dihydrates Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000002875 fluorescence polarization Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 229960000308 fosfomycin Drugs 0.000 description 1
- YMDXZJFXQJVXBF-STHAYSLISA-N fosfomycin Chemical compound C[C@@H]1O[C@@H]1P(O)(O)=O YMDXZJFXQJVXBF-STHAYSLISA-N 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229960003085 meticillin Drugs 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002887 multiple sequence alignment Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 1
- 229960001082 trimethoprim Drugs 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
- MYPYJXKWCTUITO-LYRMYLQWSA-O vancomycin(1+) Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C([O-])=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)[NH2+]C)[C@H]1C[C@](C)([NH3+])[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-O 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT0033701A AT410444B (de) | 2001-03-02 | 2001-03-02 | Verfahren zur detektion von nukleinsäuremolekülen |
| PCT/AT2002/000060 WO2002070736A2 (de) | 2001-03-02 | 2002-03-01 | Verfahren zur detektion von nukleinsäuremolekülen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SK10832003A3 SK10832003A3 (sk) | 2004-05-04 |
| SK287793B6 true SK287793B6 (sk) | 2011-10-04 |
Family
ID=3672000
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SK1083-2003A SK287793B6 (sk) | 2001-03-02 | 2002-03-01 | Spôsob stanovenia molekúl nukleových kyselín |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US20050196756A1 (https=) |
| EP (1) | EP1366195B1 (https=) |
| JP (2) | JP2004520838A (https=) |
| KR (1) | KR100892184B1 (https=) |
| AT (1) | AT410444B (https=) |
| AU (1) | AU2002238274B2 (https=) |
| CA (1) | CA2439531A1 (https=) |
| CZ (1) | CZ20032665A3 (https=) |
| DE (1) | DE50204680D1 (https=) |
| DK (1) | DK1366195T3 (https=) |
| ES (1) | ES2252427T3 (https=) |
| HU (1) | HUP0303377A3 (https=) |
| NO (1) | NO20033884L (https=) |
| PL (1) | PL365022A1 (https=) |
| SK (1) | SK287793B6 (https=) |
| WO (1) | WO2002070736A2 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003144153A (ja) * | 2001-11-09 | 2003-05-20 | Gifu Univ | 遺伝子検出方法、遺伝子検出用プライマー、dnaマイクロアレイ及び遺伝子検出用キット |
| KR100619189B1 (ko) * | 2004-10-08 | 2006-08-31 | 굿젠 주식회사 | 성교전파성질환 원인균 탐지용 프로브 및 이를 이용한성교전파성질환 원인균 유전자형 분석용 dna 칩,분석키트 및 유전형 분석방법 |
| EP1674583A1 (en) * | 2004-12-23 | 2006-06-28 | Eppendorf Array Technologies SA | Method and kit for the detection of a large number of genes related to antibiotic resistance in microorganisms |
| US20070059714A1 (en) * | 2005-09-12 | 2007-03-15 | Birgit Strommenger | Detection of presence and antibiotic susceptibility of enterococci |
| AT504194B1 (de) * | 2006-09-07 | 2008-07-15 | Oesterr Rotes Kreuz | Bakteriennachweis |
| EP2270203A1 (en) | 2009-06-29 | 2011-01-05 | AIT Austrian Institute of Technology GmbH | Oligonucleotide hybridization method |
| WO2013176767A1 (en) | 2012-05-25 | 2013-11-28 | The University Of North Carolina At Chapel Hill | Microfluidic devices, solid supports for reagents and related methods |
| CA2894632A1 (en) * | 2012-11-15 | 2014-05-22 | Todd WALLACH | Pcr reaction mixtures and methods of using same |
| CN114272966B (zh) | 2015-07-22 | 2023-11-07 | 北卡罗来纳-查佩尔山大学 | 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法 |
| CN109789409A (zh) | 2016-10-07 | 2019-05-21 | 勃林格殷格翰维特梅迪卡有限公司 | 用于测试样品的方法和分析系统 |
| DK3523448T3 (da) * | 2016-10-07 | 2021-06-28 | Boehringer Ingelheim Vetmedica Gmbh | Fremgangsmåde og analysesystem til at teste en prøve |
| EP3523030B1 (en) | 2016-10-07 | 2021-05-05 | Boehringer Ingelheim Vetmedica GmbH | Analysis system and method for testing a sample |
| EP3794080B1 (en) | 2018-05-18 | 2024-04-10 | The University of North Carolina at Chapel Hill | Compositions, devices, and methods for improving a surface property of a substrate |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1988003171A1 (en) * | 1986-10-30 | 1988-05-05 | Synergen Biologicals, Inc. | Human pancreatic secretory trypsin inhibitors produced by recombinant dna methods and processes for the production of same |
| CA1320162C (en) * | 1988-08-11 | 1993-07-13 | California Biotechnology Inc. | Method for stabilizing heterologous protein expression and vectors for use therein |
| US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| US5491225A (en) * | 1991-12-19 | 1996-02-13 | Hoffmann-La Roche Inc. | PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays |
| US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
| US6045996A (en) * | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
| US6063566A (en) * | 1994-05-13 | 2000-05-16 | The Scripps Research Institute | Catalytic RNA molecules |
| US6150093A (en) * | 1994-08-18 | 2000-11-21 | The Trustees Of Columbia University In The City Of New York | Unique associated Kaposi's sarcoma virus sequences and uses thereof |
| US6001564A (en) * | 1994-09-12 | 1999-12-14 | Infectio Diagnostic, Inc. | Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US5702895A (en) * | 1995-01-19 | 1997-12-30 | Wakunaga Seiyaku Kabushiki Kaisha | Method and kit for detecting methicillin-resistant Staphylococcus aureus |
| US5994066A (en) * | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US5612473A (en) * | 1996-01-16 | 1997-03-18 | Gull Laboratories | Methods, kits and solutions for preparing sample material for nucleic acid amplification |
| US5627054A (en) * | 1996-04-05 | 1997-05-06 | The United States Of America As Represented By The Secretary Of The Army | Competitor primer asymmetric polymerase chain reaction |
| GB9902970D0 (en) * | 1999-02-11 | 1999-03-31 | Zeneca Ltd | Novel matrix |
| GB9904804D0 (en) * | 1999-03-02 | 1999-04-28 | King S College London | Identification of bacteria |
| CN1117161C (zh) * | 1999-09-24 | 2003-08-06 | 东南大学 | 高密度基因芯片的制作方法 |
-
2001
- 2001-03-02 AT AT0033701A patent/AT410444B/de not_active IP Right Cessation
-
2002
- 2002-03-01 SK SK1083-2003A patent/SK287793B6/sk not_active IP Right Cessation
- 2002-03-01 WO PCT/AT2002/000060 patent/WO2002070736A2/de not_active Ceased
- 2002-03-01 HU HU0303377A patent/HUP0303377A3/hu unknown
- 2002-03-01 AU AU2002238274A patent/AU2002238274B2/en not_active Ceased
- 2002-03-01 CZ CZ20032665A patent/CZ20032665A3/cs unknown
- 2002-03-01 JP JP2002570758A patent/JP2004520838A/ja not_active Withdrawn
- 2002-03-01 US US10/469,713 patent/US20050196756A1/en not_active Abandoned
- 2002-03-01 KR KR1020037011418A patent/KR100892184B1/ko not_active Expired - Fee Related
- 2002-03-01 DE DE50204680T patent/DE50204680D1/de not_active Expired - Lifetime
- 2002-03-01 DK DK02704467T patent/DK1366195T3/da active
- 2002-03-01 EP EP02704467A patent/EP1366195B1/de not_active Expired - Lifetime
- 2002-03-01 PL PL02365022A patent/PL365022A1/xx not_active IP Right Cessation
- 2002-03-01 ES ES02704467T patent/ES2252427T3/es not_active Expired - Lifetime
- 2002-03-01 CA CA002439531A patent/CA2439531A1/en not_active Abandoned
-
2003
- 2003-09-02 NO NO20033884A patent/NO20033884L/no not_active Application Discontinuation
-
2010
- 2010-07-19 US US12/839,100 patent/US20100317535A1/en not_active Abandoned
- 2010-12-24 JP JP2010288416A patent/JP2011101652A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20100317535A1 (en) | 2010-12-16 |
| ATA3372001A (de) | 2002-09-15 |
| NO20033884L (no) | 2003-10-28 |
| JP2011101652A (ja) | 2011-05-26 |
| HUP0303377A2 (hu) | 2004-01-28 |
| SK10832003A3 (sk) | 2004-05-04 |
| CZ20032665A3 (cs) | 2005-02-16 |
| DK1366195T3 (da) | 2006-03-13 |
| JP2004520838A (ja) | 2004-07-15 |
| PL365022A1 (en) | 2004-12-27 |
| KR100892184B1 (ko) | 2009-04-07 |
| WO2002070736A2 (de) | 2002-09-12 |
| EP1366195B1 (de) | 2005-10-26 |
| WO2002070736A3 (de) | 2003-09-12 |
| ES2252427T3 (es) | 2006-05-16 |
| EP1366195A2 (de) | 2003-12-03 |
| WO2002070736A8 (de) | 2003-01-23 |
| DE50204680D1 (de) | 2005-12-01 |
| AU2002238274B2 (en) | 2007-06-28 |
| HUP0303377A3 (en) | 2005-12-28 |
| AT410444B (de) | 2003-04-25 |
| NO20033884D0 (no) | 2003-09-02 |
| US20050196756A1 (en) | 2005-09-08 |
| KR20030092009A (ko) | 2003-12-03 |
| CA2439531A1 (en) | 2002-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20100317535A1 (en) | Methods and Compositions For Detecting Nucleic Acid Molecules | |
| JP4860869B2 (ja) | 固相支持体上の複数のポリヌクレオチドを増幅し、検出する方法 | |
| CN102482719B (zh) | 使用阵列比较基因组杂交技术分析核酸突变的方法 | |
| EP1096024A1 (en) | Method and kit for the screening and/or the quantification of multiple homologous nucleic acid sequences on arrays | |
| CA2840558C (en) | Methods of detecting gene fusions using first and second nucleic acid probes | |
| CN110719957B (zh) | 用于核酸靶向富集的方法和试剂盒 | |
| US9175339B2 (en) | Method for detection of target nucleic acid | |
| EP1169474A1 (en) | Olignucleotide array and methods of use | |
| US6692915B1 (en) | Sequencing a polynucleotide on a generic chip | |
| CA2318371A1 (en) | Method for the detection of nucleic acid sequences | |
| JP2005143492A (ja) | 標的核酸配列の検出方法 | |
| EP1612282B1 (en) | Probe set and substrate for detecting nucleic acid | |
| US20090275028A1 (en) | Method of detecting target nucleic acid | |
| WO2016038351A1 (en) | Methods of detection of multidrug resistant bacteria | |
| CN114207146B (zh) | 组合的液相和固相dna扩增 | |
| US20120196765A1 (en) | Method for detection or analysis of target sequence in genomic dna | |
| EP1136566A1 (en) | Method and kit for detection and/or quantification of homologus nucleotide sequences on arrays | |
| JP2008259510A (ja) | 標的核酸配列の検出方法 | |
| TW202204635A (zh) | 銀色葡萄球菌之檢測用之引子組及探針 | |
| JP5378724B2 (ja) | 発現mRNA識別方法 | |
| EP1113080A2 (en) | Personal gene library | |
| HK1035213A (en) | Personal gene library | |
| JP2004201635A (ja) | nestedPCRで複数種の微生物に対する増幅を共通条件で同時に行う方法 | |
| JP2002034599A (ja) | 1塩基多型を検出する方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Patent lapsed due to non-payment of maintenance fees |
Effective date: 20120301 |