SG174471A1 - Culture of pluripotent and multipotent cells on microcarriers - Google Patents

Culture of pluripotent and multipotent cells on microcarriers Download PDF

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Publication number
SG174471A1
SG174471A1 SG2011067725A SG2011067725A SG174471A1 SG 174471 A1 SG174471 A1 SG 174471A1 SG 2011067725 A SG2011067725 A SG 2011067725A SG 2011067725 A SG2011067725 A SG 2011067725A SG 174471 A1 SG174471 A1 SG 174471A1
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SG
Singapore
Prior art keywords
cells
culture
microcarriers
passages
pluripotent
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SG2011067725A
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English (en)
Inventor
Steve Oh
Allen Chen
Andre Choo
Shaul Reuveny
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Agency Science Tech & Res
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Publication of SG174471A1 publication Critical patent/SG174471A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0607Non-embryonic pluripotent stem cells, e.g. MASC
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases [EC 2.]
    • C12N2501/727Kinases (EC 2.7.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00Microcarriers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Transplantation (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
SG2011067725A 2009-03-20 2010-03-12 Culture of pluripotent and multipotent cells on microcarriers SG174471A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16178609P 2009-03-20 2009-03-20
PCT/SG2010/000091 WO2010107392A1 (en) 2009-03-20 2010-03-12 Culture of pluripotent and multipotent cells on microcarriers

Publications (1)

Publication Number Publication Date
SG174471A1 true SG174471A1 (en) 2011-10-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
SG2011067725A SG174471A1 (en) 2009-03-20 2010-03-12 Culture of pluripotent and multipotent cells on microcarriers

Country Status (7)

Country Link
US (1) US9150829B2 (enExample)
EP (1) EP2408903B1 (enExample)
JP (1) JP2012520672A (enExample)
KR (1) KR20110137805A (enExample)
CN (1) CN102428172A (enExample)
SG (1) SG174471A1 (enExample)
WO (1) WO2010107392A1 (enExample)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0222846D0 (en) * 2002-10-03 2002-11-06 Choo Yen Cell culture
US9458431B2 (en) * 2008-03-17 2016-10-04 Agency For Science, Technology And Research Microcarriers for stem cell culture
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
CA2777552C (en) 2009-10-19 2019-05-21 Cellular Dynamics International, Inc. Cardiomyocyte production
TWI419970B (zh) * 2010-11-11 2013-12-21 Univ Nat Taiwan 使成體幹細胞產生為一球體細胞群體的方法
WO2012104087A2 (en) * 2011-02-02 2012-08-09 Cellmade Sas Cell culture device
WO2012149574A1 (en) * 2011-04-29 2012-11-01 Tissuetech, Inc. Methods of isolating cells
US20130064799A1 (en) * 2011-06-01 2013-03-14 Tariq M. Rana Methods for promoting cell reprogramming
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
EP3488878A1 (en) * 2012-03-08 2019-05-29 LifeCell Corporation Enzyme-activated collagen and tissue matrices
CN108753679A (zh) * 2012-07-24 2018-11-06 日产化学工业株式会社 培养基组合物以及使用所述组合物培养细胞或组织的方法
AU2013248265B2 (en) * 2012-11-08 2018-11-01 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
CN103031270A (zh) * 2013-01-05 2013-04-10 绍兴文理学院 胆管上皮细胞的高效扩增和培养方法
EP2986711A1 (en) * 2013-04-16 2016-02-24 Universiteit Maastricht Blastoid, cell line based artificial blastocyst
CN103305453A (zh) * 2013-05-23 2013-09-18 侯亚义 一种脐带间充质干细胞的微载体培养系统
WO2015008275A1 (en) * 2013-07-17 2015-01-22 Kadimastem Ltd Methods for large scale generation of stem cells
KR101662770B1 (ko) * 2013-11-01 2016-10-06 건국대학교 글로컬산학협력단 하이브리드 줄기세포 및 만능성 줄기세포의 생존율을 높이는 방법
KR101845588B1 (ko) * 2014-04-07 2018-04-05 한양대학교 산학협력단 적혈구계 세포의 인 비트로 확장
WO2015195780A1 (en) * 2014-06-17 2015-12-23 Lieber Institute For Brain Development Assay for cell specification in self-renewing human pluripotent stem cells
FR3032202B1 (fr) 2015-02-03 2018-12-07 Maco Pharma Matrice sous forme de bille comme support cellulaire
US20180066231A1 (en) * 2015-02-27 2018-03-08 Rohto Pharmaceutical Co., Ltd. Mesenchymal stem cell culture medium, methods for culturing mesenchymal stem cells, and mesenchymal stem cells
US20190127707A1 (en) * 2016-03-30 2019-05-02 Kyowa Hakko Bio Co., Ltd. Medium for culturing naive pluripotent stem cells and method for culturing pluripotent stem cells
CN106282094B (zh) * 2016-10-13 2018-11-27 吴欣怡 人皮肤来源的前体细胞诱导分化为角膜内皮样细胞的方法
WO2018075940A1 (en) 2016-10-21 2018-04-26 Georgia Tech Research Corporation Methods and systems for t cell expansion
FR3059009B1 (fr) 2016-11-23 2018-12-07 Universite de Bordeaux Microcompartiment cellulaire et procedes de preparation
CN108121161A (zh) * 2016-11-26 2018-06-05 中国科学院大连化学物理研究所 一种高通量形成拟胚体的微阵列芯片的制备方法与应用
US20200283726A1 (en) * 2017-11-21 2020-09-10 Georgetown University Process for continuous cell culture of gpscs
WO2020080561A1 (ko) * 2018-10-15 2020-04-23 (주)메디톡스 만능성 줄기세포 배양용 배지 및 그를 이용한 배양 방법
AU2019368313A1 (en) * 2018-10-24 2021-05-20 Hebecell Corporation Methods and systems for manufacturing hematopoietic lineage cells
KR102120378B1 (ko) * 2019-07-04 2020-06-08 연세대학교 산학협력단 암 오가노이드의 제조 방법 및 이의 용도
WO2021087354A1 (en) * 2019-10-30 2021-05-06 The General Hospital Corporation High-throughput culture of ipsc-derived alveolar cells
WO2021162206A1 (ko) * 2020-02-10 2021-08-19 주식회사 마이크로바이오틱스 모낭조직 배양액을 포함하는 탈모의 예방 또는 치료용 조성물
KR102458606B1 (ko) * 2020-03-06 2022-10-25 경희대학교 산학협력단 마이크로캐리어 기반 4차원 세포 배양기 및 이를 이용한 세포배양 모니터링 방법
EP4130244A4 (en) * 2020-03-24 2024-05-29 Jichi Medical University TEMPORARY TREATMENT MEDIUM, TREATMENT KIT, EMBRYO DEVELOPMENT ARREST INHIBITOR, METHOD OF PREPARATION
CA3181611A1 (en) * 2020-06-11 2021-12-16 Paul Simmons 3d culture of mesenchymal lineage precursor or stem cells
JP2023537969A (ja) * 2020-08-10 2023-09-06 リネージ セル セラピューティクス インコーポレイテッド 胚性幹細胞増殖のための組成物及び方法
CN114763534B (zh) * 2021-01-13 2024-04-26 合肥中科普瑞昇生物医药科技有限公司 原代胃肠间质瘤细胞培养基、培养方法及其应用
CN112843338B (zh) * 2021-02-08 2022-05-20 康膝生物医疗(深圳)有限公司 一种制备含胶原蛋白的间充质干细胞注射剂的方法
CN113201488A (zh) * 2021-05-13 2021-08-03 圣美基因科技(北京)有限公司 一种间充质干细胞大规模培养方法
CN113388582A (zh) * 2021-06-21 2021-09-14 香港再生医学有限公司 促使iPSC分化为外周神经干细胞的方法及培养基和系统
TWI861626B (zh) * 2021-12-21 2024-11-11 國璽幹細胞應用技術股份有限公司 穩定擴增富潛能幹細胞的方法
WO2023123299A1 (en) * 2021-12-31 2023-07-06 Beijing Theraxyte Bioscience Co., Ltd. Compositions and methods for culturing stem cells
CN115747197B (zh) * 2023-01-06 2023-04-21 中国肉类食品综合研究中心 可食用3d打印生物墨水及制备方法和在培育肉中的应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5853717A (en) 1994-07-20 1998-12-29 Cytotherapeutics, Inc. Methods and compositions of growth control for cells encapsulated within bioartificial organs
ATE307195T1 (de) 1997-07-14 2005-11-15 Osiris Therapeutics Inc Herzmuskelregenerierung unter verwendung mesenchymaler stammzellen
JP2005506839A (ja) 2001-10-02 2005-03-10 ベクトン・ディキンソン・アンド・カンパニー 細胞外マトリックスおよび他の分子を使用する幹細胞の増殖および分化
WO2005007799A2 (en) * 2003-07-17 2005-01-27 Gamida-Cell Ltd. Methods for ex-vivo expanding stem/progenitor cells
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
WO2006084040A2 (en) * 2005-02-04 2006-08-10 Massachusetts Institute Of Technology Engineering vascularized muscle tissue
WO2006091921A2 (en) 2005-02-25 2006-08-31 Drexel University Super-sparger microcarrier beads and precision extrusion deposited poly-epsilon-caprolactone structures for biological applications
WO2007030469A2 (en) 2005-09-06 2007-03-15 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Transplantable cell growth niche and related compositions and methods
AU2006292021B2 (en) 2005-09-12 2012-05-31 Es Cell International Pte Ltd. Cardiomyocyte production
AU2006326853B2 (en) 2005-12-22 2012-02-16 Es Cell International Pte Ltd Direct differentiation of cardiomyocytes from human embryonic stem cells
WO2007149926A1 (en) 2006-06-20 2007-12-27 Wisconsin Alumni Research Foundation Method for culturing stem cells
WO2008004990A2 (en) 2006-07-06 2008-01-10 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
CA2657013A1 (en) 2006-07-06 2008-01-10 Organogenesis Inc. Temperature-responsive microcarrier
ES2704401T3 (es) 2006-08-02 2019-03-18 Technion Res & Dev Foundation Métodos de expansión de células madre embrionarias en un cultivo en suspensión
JP2008099662A (ja) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
CA2675445C (en) * 2007-01-17 2014-11-04 Wisconsin Alumni Research Foundation Improved culture of stem cells
DK2173863T3 (en) * 2007-06-29 2019-01-21 Fujifilm Cellular Dynamics Inc Automated method and apparatus for embryonic stem cell culture
US8828720B2 (en) 2008-03-17 2014-09-09 Agency For Science, Technology And Research Microcarriers for stem cell culture
US20110143433A1 (en) 2008-03-17 2011-06-16 Agency For Science, Technology And Research Microcarriers for Stem Cell Culture
US8691569B2 (en) 2008-03-17 2014-04-08 Agency For Science, Technology And Research Microcarriers for stem cell culture
SG188918A1 (enExample) 2008-03-17 2013-04-30 Agency Science Tech & Res
WO2009139703A1 (en) 2008-05-15 2009-11-19 Ge Healthcare Bio-Sciences Ab Method for cell expansion
KR101651661B1 (ko) 2008-06-30 2016-08-26 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
PL2366021T3 (pl) 2008-11-20 2017-12-29 Janssen Biotech, Inc. Hodowla pluripotencjalnych komórek macierzystych na mikronośnikach

Also Published As

Publication number Publication date
JP2012520672A (ja) 2012-09-10
EP2408903B1 (en) 2014-08-06
CN102428172A (zh) 2012-04-25
KR20110137805A (ko) 2011-12-23
EP2408903A4 (en) 2013-02-20
WO2010107392A1 (en) 2010-09-23
US9150829B2 (en) 2015-10-06
EP2408903A1 (en) 2012-01-25
US20120009645A1 (en) 2012-01-12

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