SG11202106445RA - One pot synthesis of sets of oligonucleotides - Google Patents
One pot synthesis of sets of oligonucleotidesInfo
- Publication number
- SG11202106445RA SG11202106445RA SG11202106445RA SG11202106445RA SG11202106445RA SG 11202106445R A SG11202106445R A SG 11202106445RA SG 11202106445R A SG11202106445R A SG 11202106445RA SG 11202106445R A SG11202106445R A SG 11202106445RA SG 11202106445R A SG11202106445R A SG 11202106445RA
- Authority
- SG
- Singapore
- Prior art keywords
- oligonucleotides
- sets
- pot synthesis
- pot
- synthesis
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19305007 | 2019-01-03 | ||
| PCT/EP2019/087048 WO2020141143A1 (en) | 2019-01-03 | 2019-12-26 | One pot synthesis of sets of oligonucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202106445RA true SG11202106445RA (en) | 2021-07-29 |
Family
ID=65200741
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202106445RA SG11202106445RA (en) | 2019-01-03 | 2019-12-26 | One pot synthesis of sets of oligonucleotides |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20220356510A1 (zh) |
| EP (1) | EP3906317A1 (zh) |
| JP (1) | JP7491934B2 (zh) |
| KR (1) | KR20210111262A (zh) |
| CN (1) | CN113272442B (zh) |
| AU (1) | AU2019417966A1 (zh) |
| CA (1) | CA3124763A1 (zh) |
| IL (1) | IL284441A (zh) |
| SG (1) | SG11202106445RA (zh) |
| WO (1) | WO2020141143A1 (zh) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3145912A1 (en) * | 2019-08-01 | 2021-02-04 | Adrian Horgan | Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides. |
| JP2022549196A (ja) | 2019-09-23 | 2022-11-24 | ディーエヌエー スクリプト | ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加 |
| WO2022013094A1 (en) | 2020-07-15 | 2022-01-20 | Dna Script | Massively parallel enzymatic synthesis of polynucleotides |
| US20220064206A1 (en) * | 2020-08-28 | 2022-03-03 | Twist Bioscience Corporation | Devices and methods for synthesis |
| US20220195476A1 (en) * | 2020-12-21 | 2022-06-23 | Chen cheng yao | Method and kit for regenerating reusable initiators for nucleic acid synthesis |
| CN118127102A (zh) * | 2024-05-10 | 2024-06-04 | 天津凯莱英生物科技有限公司 | 酶法合成具有3’-o-磷酸修饰的核酸的方法 |
Family Cites Families (57)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5367066A (en) | 1984-10-16 | 1994-11-22 | Chiron Corporation | Oligonucleotides with selectably cleavable and/or abasic sites |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US4942124A (en) | 1987-08-11 | 1990-07-17 | President And Fellows Of Harvard College | Multiplex sequencing |
| US5168038A (en) | 1988-06-17 | 1992-12-01 | The Board Of Trustees Of The Leland Stanford Junior University | In situ transcription in cells and tissues |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| EP0450060A1 (en) | 1989-10-26 | 1991-10-09 | Sri International | Dna sequencing |
| US6054034A (en) | 1990-02-28 | 2000-04-25 | Aclara Biosciences, Inc. | Acrylic microchannels and their use in electrophoretic applications |
| US5126022A (en) | 1990-02-28 | 1992-06-30 | Soane Tecnologies, Inc. | Method and device for moving molecules by the application of a plurality of electrical fields |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| JP3080178B2 (ja) | 1991-02-18 | 2000-08-21 | 東洋紡績株式会社 | 核酸配列の増幅方法およびそのための試薬キット |
| US5981179A (en) | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
| US5498392A (en) | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5436143A (en) | 1992-12-23 | 1995-07-25 | Hyman; Edward D. | Method for enzymatic synthesis of oligonucleotides |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| DE69531545T2 (de) | 1994-06-23 | 2004-06-24 | Affymax Technologies N.V., Willemstad | Photolabile verbindungen und verfahren für deren verwendung |
| US6001229A (en) | 1994-08-01 | 1999-12-14 | Lockheed Martin Energy Systems, Inc. | Apparatus and method for performing microfluidic manipulations for chemical analysis |
| US5808045A (en) | 1994-09-02 | 1998-09-15 | Andrew C. Hiatt | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
| US5763594A (en) | 1994-09-02 | 1998-06-09 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
| US5604097A (en) | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| US5700642A (en) | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
| US5830655A (en) | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6458530B1 (en) | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
| ATE295427T1 (de) | 1996-06-04 | 2005-05-15 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| CA2306126A1 (en) | 1997-10-15 | 1999-04-22 | Aclara Biosciences, Inc. | Laminate microstructure device and method for making same |
| DE69928995T2 (de) | 1998-06-22 | 2006-09-07 | Affymetrix, Inc., Santa Clara | Reagenz und Verfahren zu fester Phase Synthese |
| JP3974441B2 (ja) | 1998-11-09 | 2007-09-12 | 栄研化学株式会社 | 核酸の合成方法 |
| EP1159285B1 (en) | 1999-03-08 | 2005-05-25 | Metrigen, Inc. | Methods and compositions for economically synthesizing and assembling long dna sequences |
| CA2373537A1 (en) | 1999-05-12 | 2000-11-16 | Aclara Biosciences, Inc. | Multiplexed fluorescent detection in microfluidic devices |
| US6939451B2 (en) | 2000-09-19 | 2005-09-06 | Aclara Biosciences, Inc. | Microfluidic chip having integrated electrodes |
| AU2002307152A1 (en) | 2001-04-06 | 2002-10-21 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| WO2004005667A1 (en) | 2002-07-08 | 2004-01-15 | Shell Internationale Research Maatschappij B.V. | Choke for controlling the flow of drilling mud |
| US7947817B2 (en) | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US8212020B2 (en) | 2005-03-11 | 2012-07-03 | Steven Albert Benner | Reagents for reversibly terminating primer extension |
| US7544794B1 (en) | 2005-03-11 | 2009-06-09 | Steven Albert Benner | Method for sequencing DNA and RNA by synthesis |
| US7537897B2 (en) | 2006-01-23 | 2009-05-26 | Population Genetics Technologies, Ltd. | Molecular counting |
| GB0612508D0 (en) | 2006-06-23 | 2006-08-02 | Arrow Int Ltd | Crystalline duloxetine hydrochloride |
| US8399188B2 (en) | 2006-09-28 | 2013-03-19 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| EP2183390A1 (en) * | 2007-08-23 | 2010-05-12 | Novartis Ag | Methods for detecting oligonucleotides |
| US8034923B1 (en) | 2009-03-27 | 2011-10-11 | Steven Albert Benner | Reagents for reversibly terminating primer extension |
| US8574864B2 (en) * | 2009-11-05 | 2013-11-05 | Epicentre Technologies Corporation | Methods and kits for 3'-end-tagging of RNA |
| US8808989B1 (en) * | 2013-04-02 | 2014-08-19 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| EP3209781B8 (en) * | 2014-10-20 | 2020-01-15 | Molecular Assemblies Inc. | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| GB201502152D0 (en) | 2015-02-10 | 2015-03-25 | Nuclera Nucleics Ltd | Novel use |
| GB201512372D0 (en) * | 2015-07-15 | 2015-08-19 | Nuclera Nucleics Ltd | Novel method |
| WO2017156218A1 (en) * | 2016-03-11 | 2017-09-14 | President And Fellows Of Harvard College | Method of making polynucleotides using closed-loop verification |
| FR3052462A1 (fr) | 2016-06-14 | 2017-12-15 | Dna Script | Variants d'une adn polymerase de la famille polx |
| WO2018152323A1 (en) * | 2017-02-17 | 2018-08-23 | Camena Bioscience Limited | Compositions and methods for template-free enzymatic nucleic acid synthesis |
| WO2019051253A1 (en) | 2017-09-08 | 2019-03-14 | Sigma-Aldrich Co. Llc | DNA MODIFIED POLYMERASES |
| EP3737749A1 (en) | 2018-01-08 | 2020-11-18 | DNA Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
-
2019
- 2019-12-26 US US17/419,637 patent/US20220356510A1/en active Pending
- 2019-12-26 SG SG11202106445RA patent/SG11202106445RA/en unknown
- 2019-12-26 CN CN201980087736.8A patent/CN113272442B/zh active Active
- 2019-12-26 CA CA3124763A patent/CA3124763A1/en active Pending
- 2019-12-26 JP JP2021538753A patent/JP7491934B2/ja active Active
- 2019-12-26 AU AU2019417966A patent/AU2019417966A1/en active Pending
- 2019-12-26 EP EP19829643.6A patent/EP3906317A1/en active Pending
- 2019-12-26 KR KR1020217022202A patent/KR20210111262A/ko active Search and Examination
- 2019-12-26 WO PCT/EP2019/087048 patent/WO2020141143A1/en unknown
-
2021
- 2021-06-28 IL IL284441A patent/IL284441A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022515912A (ja) | 2022-02-22 |
| AU2019417966A1 (en) | 2021-07-08 |
| CA3124763A1 (en) | 2020-07-09 |
| CN113272442A (zh) | 2021-08-17 |
| WO2020141143A1 (en) | 2020-07-09 |
| CN113272442B (zh) | 2024-03-15 |
| JP7491934B2 (ja) | 2024-05-28 |
| IL284441A (en) | 2021-08-31 |
| KR20210111262A (ko) | 2021-09-10 |
| EP3906317A1 (en) | 2021-11-10 |
| US20220356510A1 (en) | 2022-11-10 |
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