SG11202009744WA - Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample - Google Patents

Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

Info

Publication number
SG11202009744WA
SG11202009744WA SG11202009744WA SG11202009744WA SG11202009744WA SG 11202009744W A SG11202009744W A SG 11202009744WA SG 11202009744W A SG11202009744W A SG 11202009744WA SG 11202009744W A SG11202009744W A SG 11202009744WA SG 11202009744W A SG11202009744W A SG 11202009744WA
Authority
SG
Singapore
Prior art keywords
microbes
sample
types
nucleic acid
acid molecules
Prior art date
Application number
SG11202009744WA
Other languages
English (en)
Inventor
Suneer Jain
Original Assignee
Sun Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sun Genomics Inc filed Critical Sun Genomics Inc
Publication of SG11202009744WA publication Critical patent/SG11202009744WA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/125Specific component of sample, medium or buffer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/137Concentration of a component of medium
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11202009744WA 2018-04-02 2019-04-02 Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample SG11202009744WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862651620P 2018-04-02 2018-04-02
PCT/US2019/025457 WO2019195342A1 (en) 2018-04-02 2019-04-02 Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

Publications (1)

Publication Number Publication Date
SG11202009744WA true SG11202009744WA (en) 2020-10-29

Family

ID=68101118

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202009744WA SG11202009744WA (en) 2018-04-02 2019-04-02 Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

Country Status (9)

Country Link
JP (2) JP2021520191A (ko)
KR (1) KR20200140303A (ko)
CN (1) CN112204139A (ko)
AU (1) AU2019247693A1 (ko)
CA (1) CA3095745A1 (ko)
GB (1) GB2587545B (ko)
IL (1) IL277682A (ko)
SG (1) SG11202009744WA (ko)
WO (1) WO2019195342A1 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113186185B (zh) * 2020-01-14 2023-05-26 东北林业大学 一种从哺乳动物粪便中高效富集宿主dna的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2269129T3 (es) * 1999-04-09 2007-04-01 Exact Sciences Corporation Procedimientos para detectar acidos nucleicos reveladores de cancer.
KR20050103085A (ko) * 2004-04-24 2005-10-27 한국생명공학연구원 유산균을 검출하기 위한 게놈 마이크로어레이 및 이를이용한 유산균의 검출 방법
WO2012043183A1 (ja) * 2010-09-28 2012-04-05 オリンパス株式会社 糞便中の標的核酸の合成方法
LU91864B1 (en) * 2011-09-02 2013-03-04 Univ Luxembourg Method and kit for the isolation of genomic DNA, RNA, proteins and metabolites from a single biological sample
US9145553B2 (en) * 2012-06-15 2015-09-29 Ut-Battelle, Llc Method for isolating nucleic acids
WO2014029791A1 (en) * 2012-08-21 2014-02-27 Qiagen Gmbh Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
ITMI20131473A1 (it) * 2013-09-06 2015-03-07 Sofar Spa Metodo per valutare gli effetti di una composizione comprendente microrganismi sul microbiota intestinale
CN106460048B (zh) * 2014-04-28 2021-01-05 耶达研究及发展有限公司 针对试剂的微生物组应答
CN107075588B (zh) * 2014-10-21 2023-03-21 普梭梅根公司 用于微生物组来源的诊断和治疗的方法及系统
US11773455B2 (en) * 2015-09-09 2023-10-03 Psomagen, Inc. Method and system for microbiome-derived diagnostics and therapeutics infectious disease and other health conditions associated with antibiotic usage
CN108368542B (zh) * 2015-10-19 2022-04-08 多弗泰尔基因组学有限责任公司 用于基因组组装、单元型定相以及独立于靶标的核酸检测的方法
CA3036702A1 (en) * 2016-09-15 2018-03-22 Sun Genomics Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US10428370B2 (en) * 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

Also Published As

Publication number Publication date
JP2024063020A (ja) 2024-05-10
CA3095745A1 (en) 2019-10-10
GB2587545B (en) 2023-06-14
AU2019247693A1 (en) 2020-11-19
KR20200140303A (ko) 2020-12-15
EP3775197A1 (en) 2021-02-17
GB202017361D0 (en) 2020-12-16
WO2019195342A1 (en) 2019-10-10
IL277682A (en) 2020-11-30
CN112204139A (zh) 2021-01-08
GB2587545A (en) 2021-03-31
JP2021520191A (ja) 2021-08-19

Similar Documents

Publication Publication Date Title
IL265285B (en) A universal method for extracting nucleic acid molecules from a diverse population of one or more types of bacteria in an example
IL282421A (en) A universal method for extracting nucleic acid molecules from a diverse population of bacteria
SG11202108788TA (en) Methods for processing nucleic acid molecules
ZA202210297B (en) Genomic infrastructure for on-site or cloud-based dna and rna processing and analysis
AU2017263886A1 (en) Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids
HK1256342A1 (zh) 環境或生物樣本中的核酸純化
EP3845640A3 (en) Modified tranposases for improved insertion sequence bias and increased dna input tolerence
EP3734294A4 (en) METHOD OF EXTRACTION OF NUCLEIC ACID USING A CARTRIDGE
EP3167062A4 (en) Methods for isolating microvesicles and extracting nucleic acids from biological samples
EP3201350A4 (en) Method for nucleic acid analysis directly from an unpurified biological sample
IL260800B (en) A test tube for shaking using spheres and a method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
EP3559231A4 (en) METHOD FOR PROCESSING NUCLEIC ACID SAMPLES
IL290274A (en) Methods and reagents for nucleic acid sequencing and related applications
EP3192900A4 (en) Method for constructing nucleic acid single-stranded cyclic library and reagents thereof
GB2550712B (en) Method for constructing hairpin-containing nucleic acid molecules for nanopore sequencing
GB2613427B (en) Methods and Systems for Analyzing Nucleic Acid Molecules
SG11202003885UA (en) Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
EP3192901A4 (en) Method for constructing nucleic acid single-stranded cyclic library and reagents thereof
EP3512965C0 (en) METHOD FOR THE PRODUCTION OF NUCLEIC ACIDS FOR THE ANALYSIS OF CELL-FREE DNA
EP3505628A4 (en) METHOD FOR CONCENTRATING MICROORGANISMS OR FOR EXTRACING NUCLEIC ACID USING DTBP
WO2016109799A3 (en) Method of rna isolation from clinical samples
IL283775A (en) Trilop-containing double-stranded nucleic acid inhibitory molecules
ZA201905052B (en) Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
HK1250246A1 (zh) 使用靶向特異性dna探針在生物樣品中進行染色體多重分析的方法和系統
IL277682A (en) A universal method for extracting nucleic acid molecules from a diverse population of one or more types of bacteria in a sample