CN112204139A - 用于从样品中的一种或多种类型的微生物的不同群体中提取核酸分子的通用方法 - Google Patents

用于从样品中的一种或多种类型的微生物的不同群体中提取核酸分子的通用方法 Download PDF

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CN112204139A
CN112204139A CN201980036347.2A CN201980036347A CN112204139A CN 112204139 A CN112204139 A CN 112204139A CN 201980036347 A CN201980036347 A CN 201980036347A CN 112204139 A CN112204139 A CN 112204139A
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genetic material
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S·杰恩
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Sun Genomics Inc
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Sun Genomics Inc
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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    • C12Q2527/125Specific component of sample, medium or buffer
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/137Concentration of a component of medium
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
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    • G16B20/00ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10Sequence alignment; Homology search

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980036347.2A 2018-04-02 2019-04-02 用于从样品中的一种或多种类型的微生物的不同群体中提取核酸分子的通用方法 Pending CN112204139A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862651620P 2018-04-02 2018-04-02
US62/651,620 2018-04-02
PCT/US2019/025457 WO2019195342A1 (en) 2018-04-02 2019-04-02 Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

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CN112204139A true CN112204139A (zh) 2021-01-08

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CN201980036347.2A Pending CN112204139A (zh) 2018-04-02 2019-04-02 用于从样品中的一种或多种类型的微生物的不同群体中提取核酸分子的通用方法

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JP (1) JP2021520191A (ko)
KR (1) KR20200140303A (ko)
CN (1) CN112204139A (ko)
AU (1) AU2019247693A1 (ko)
CA (1) CA3095745A1 (ko)
GB (1) GB2587545B (ko)
IL (1) IL277682A (ko)
SG (1) SG11202009744WA (ko)
WO (1) WO2019195342A1 (ko)

Families Citing this family (1)

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CN113186185B (zh) * 2020-01-14 2023-05-26 东北林业大学 一种从哺乳动物粪便中高效富集宿主dna的方法

Citations (9)

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JP2002541824A (ja) * 1999-04-09 2002-12-10 エグザクト サイエンシーズ コーポレイション 癌の指標である核酸を検出するための方法
KR20050103085A (ko) * 2004-04-24 2005-10-27 한국생명공학연구원 유산균을 검출하기 위한 게놈 마이크로어레이 및 이를이용한 유산균의 검출 방법
WO2012043183A1 (ja) * 2010-09-28 2012-04-05 オリンパス株式会社 糞便中の標的核酸の合成方法
US20130338350A1 (en) * 2012-06-15 2013-12-19 Ut-Battelle, Llc Method for isolating nucleic acids
CN105518150A (zh) * 2013-09-06 2016-04-20 索发股份公司 用于评价包含微生物的组合物对肠道微生物群的作用的方法
US20160110515A1 (en) * 2014-10-21 2016-04-21 Zachary Apte Method and system for microbiome-derived diagnostics and therapeutics
CN106460048A (zh) * 2014-04-28 2017-02-22 耶达研究及发展有限公司 针对试剂的微生物组应答
WO2018053308A1 (en) * 2016-09-15 2018-03-22 Sun Genomics Llc Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US20180080065A1 (en) * 2016-09-15 2018-03-22 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

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LU91864B1 (en) * 2011-09-02 2013-03-04 Univ Luxembourg Method and kit for the isolation of genomic DNA, RNA, proteins and metabolites from a single biological sample
US20150225712A1 (en) * 2012-08-21 2015-08-13 Qiagen Gmbh Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
US11773455B2 (en) * 2015-09-09 2023-10-03 Psomagen, Inc. Method and system for microbiome-derived diagnostics and therapeutics infectious disease and other health conditions associated with antibiotic usage
WO2017070123A1 (en) * 2015-10-19 2017-04-27 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection

Patent Citations (9)

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Publication number Priority date Publication date Assignee Title
JP2002541824A (ja) * 1999-04-09 2002-12-10 エグザクト サイエンシーズ コーポレイション 癌の指標である核酸を検出するための方法
KR20050103085A (ko) * 2004-04-24 2005-10-27 한국생명공학연구원 유산균을 검출하기 위한 게놈 마이크로어레이 및 이를이용한 유산균의 검출 방법
WO2012043183A1 (ja) * 2010-09-28 2012-04-05 オリンパス株式会社 糞便中の標的核酸の合成方法
US20130338350A1 (en) * 2012-06-15 2013-12-19 Ut-Battelle, Llc Method for isolating nucleic acids
CN105518150A (zh) * 2013-09-06 2016-04-20 索发股份公司 用于评价包含微生物的组合物对肠道微生物群的作用的方法
CN106460048A (zh) * 2014-04-28 2017-02-22 耶达研究及发展有限公司 针对试剂的微生物组应答
US20160110515A1 (en) * 2014-10-21 2016-04-21 Zachary Apte Method and system for microbiome-derived diagnostics and therapeutics
WO2018053308A1 (en) * 2016-09-15 2018-03-22 Sun Genomics Llc Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US20180080065A1 (en) * 2016-09-15 2018-03-22 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

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松木吏弓: "DNA鑑定を利用した野生動物調査法を開発", pages 4, Retrieved from the Internet <URL:https://criepi.denken.or.jp/koho/news/den441.pdf> *

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GB2587545B (en) 2023-06-14
GB2587545A (en) 2021-03-31
AU2019247693A1 (en) 2020-11-19
CA3095745A1 (en) 2019-10-10
WO2019195342A1 (en) 2019-10-10
SG11202009744WA (en) 2020-10-29
KR20200140303A (ko) 2020-12-15
IL277682A (en) 2020-11-30
EP3775197A1 (en) 2021-02-17
GB202017361D0 (en) 2020-12-16
JP2021520191A (ja) 2021-08-19

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