SG11201809961TA - Variants of a dna polymerase of the polx family - Google Patents
Variants of a dna polymerase of the polx familyInfo
- Publication number
- SG11201809961TA SG11201809961TA SG11201809961TA SG11201809961TA SG11201809961TA SG 11201809961T A SG11201809961T A SG 11201809961TA SG 11201809961T A SG11201809961T A SG 11201809961TA SG 11201809961T A SG11201809961T A SG 11201809961TA SG 11201809961T A SG11201809961T A SG 11201809961TA
- Authority
- SG
- Singapore
- Prior art keywords
- variants
- dna polymerase
- polx family
- nucleic acid
- polx
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1655475A FR3052462A1 (fr) | 2016-06-14 | 2016-06-14 | Variants d'une adn polymerase de la famille polx |
| PCT/FR2017/051519 WO2017216472A2 (fr) | 2016-06-14 | 2017-06-13 | Variants d'une adn polymérase de la famille polx |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201809961TA true SG11201809961TA (en) | 2018-12-28 |
Family
ID=57348782
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10202009688QA SG10202009688QA (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
| SG11201809961TA SG11201809961TA (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10202009688QA SG10202009688QA (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20200002690A1 (fr) |
| EP (1) | EP3469075A2 (fr) |
| JP (2) | JP7118510B2 (fr) |
| KR (1) | KR102522263B1 (fr) |
| CN (1) | CN109477080A (fr) |
| AU (2) | AU2017286477C1 (fr) |
| CA (1) | CA3024184A1 (fr) |
| FR (2) | FR3052462A1 (fr) |
| IL (1) | IL263503A (fr) |
| SG (2) | SG10202009688QA (fr) |
| WO (1) | WO2017216472A2 (fr) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10774316B2 (en) * | 2014-10-20 | 2020-09-15 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| US20210164008A1 (en) * | 2017-05-26 | 2021-06-03 | Nuclera Nucleics Ltd. | Use of Terminal Transferase Enzyme in Nucleic Acid Synthesis |
| EP3665274A1 (fr) | 2017-08-07 | 2020-06-17 | DNA Script | Variants de l'adn polymérase de la famille a et leurs utilisations |
| US10752887B2 (en) | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| AU2019205606A1 (en) * | 2018-01-08 | 2020-07-30 | Centre National De La Recherche Scientifique | Variants of Terminal deoxynucleotidyl Transferase and uses thereof |
| JP7430701B2 (ja) | 2018-07-23 | 2024-02-13 | ディーエヌエー スクリプト | 核酸鎖の大規模並列酵素合成 |
| AU2019379287A1 (en) * | 2018-11-14 | 2021-06-03 | Dna Script | Terminal deoxynucleotidyl transferase variants and uses thereof |
| US11268091B2 (en) | 2018-12-13 | 2022-03-08 | Dna Script Sas | Direct oligonucleotide synthesis on cells and biomolecules |
| US20220356510A1 (en) | 2019-01-03 | 2022-11-10 | Dna Script | One Pot Synthesis of Sets of Oligonucleotides |
| GB201901501D0 (en) * | 2019-02-04 | 2019-03-27 | Nuclera Nucleics Ltd | Modified terminal deoxynucleotidyl transferase (TdT) enzymes |
| EP3924500A1 (fr) * | 2019-02-12 | 2021-12-22 | DNA Script | Clivage efficace de produit dans la synthèse enzymatique sans matrice de polynucléotides |
| CA3140498A1 (fr) * | 2019-05-28 | 2020-12-03 | Dna Script | Variants de la desoxynucleotidyle transferase terminale et leurs utilisations |
| EP3744854A1 (fr) * | 2019-05-28 | 2020-12-02 | DNA Script | Variants de désoxynucléotidyle transférase terminale et leurs utilisations |
| AU2020319829A1 (en) | 2019-07-30 | 2022-02-24 | Dna Script | Template-free enzymatic synthesis of polynucleotides using poly(A) and poly(U) polymerases |
| CN114430778A (zh) | 2019-08-01 | 2022-05-03 | Dna斯克瑞普特公司 | 增加多核苷酸的无模板酶促合成中长序列的产量 |
| CN110331136B (zh) * | 2019-09-05 | 2019-12-24 | 中国科学院天津工业生物技术研究所 | 一种末端脱氧核糖核苷转移酶变体及其应用 |
| JP2022547918A (ja) | 2019-09-09 | 2022-11-16 | ディーエヌエー スクリプト | 光開裂性結合を用いる鋳型なし酵素的ポリヌクレオチド合成 |
| WO2021058438A1 (fr) | 2019-09-23 | 2021-04-01 | Dna Script | Augmentation de rendements de séquence longue dans la synthèse enzymatique sans matrice de polynucléotides |
| CN114761575A (zh) | 2019-11-13 | 2022-07-15 | Dna斯克瑞普特公司 | 多核苷酸的高效无模板酶促合成 |
| EP4073240A1 (fr) * | 2019-12-12 | 2022-10-19 | DNA Script | Désoxynucléotidyltransférases terminales chimériques pour la synthèse enzymatique sans matrice de polynucléotides |
| WO2021122539A1 (fr) | 2019-12-16 | 2021-06-24 | Dna Script | Synthèse de polynucléotides enzymatiques sans matrice à l'aide de variants de désoxynucléotidyltransférases terminales sans dismutation |
| US20230089448A1 (en) | 2020-02-25 | 2023-03-23 | Dna Script | Method And Apparatus for Enzymatic Synthesis of Polynucleotides |
| EP4139446A1 (fr) | 2020-04-20 | 2023-03-01 | DNA Script | Variants de désoxynucléotidyl transférase et utilisations correspondantes |
| WO2021250265A2 (fr) | 2020-06-12 | 2021-12-16 | Synhelix | Synthèse d'acides nucléiques ab-initio indépendante de la matrice, à l'aide d'enzymes thermostables |
| WO2021254934A1 (fr) | 2020-06-16 | 2021-12-23 | Dna Script | Systèmes, appareil et kits pour la synthèse enzymatique de polynucléotides |
| US20230313255A1 (en) | 2020-07-15 | 2023-10-05 | Dna Script | Massively Parallel Enzymatic Synthesis of Polynucleotides |
| WO2022029427A1 (fr) * | 2020-08-04 | 2022-02-10 | Nuclera Nucleics Ltd | Enzymes de désoxynucléotidyl transférase terminale (tdt) modifiées |
| GB2598152A (en) * | 2020-08-21 | 2022-02-23 | Nuclera Nucleics Ltd | Modified terminal deoxynucleotidyl transferase (TdT) enzymes |
| EP4217476A1 (fr) | 2020-09-22 | 2023-08-02 | DNA Script | Variants stabilisés de désoxynucléotidyl transférase terminale tronqués à l'extrémité n-terminale et utilisations associées |
| EP4237573A1 (fr) | 2020-10-29 | 2023-09-06 | DNA Script | Synthèse enzymatique de sondes polynucléotidiques |
| US20220145345A1 (en) | 2020-11-11 | 2022-05-12 | Microsoft Technology Licensing, Llc | Spatial control of polynucleotide synthesis by strand capping |
| CN112322715B (zh) * | 2020-11-17 | 2022-11-25 | 清华大学 | 一种核酸测序方法 |
| JP2024511874A (ja) | 2021-04-02 | 2024-03-15 | ディーエヌエー スクリプト | Ｇ４を形成しやすいポリヌクレオチドの酵素合成のための方法及びキット |
| DE102022114351A1 (de) | 2021-06-10 | 2022-12-15 | Dna Script | Enzymatische synthese von polynukleotiden mittels 3'-o-amino-2'-deoxyribonukleosid triphosphat monomeren |
| WO2023025488A1 (fr) | 2021-08-23 | 2023-03-02 | Dna Script | Procédé de génération d'acide nucléique double brin |
| WO2023035003A1 (fr) | 2021-09-03 | 2023-03-09 | Elegen Corp. | Dispositifs de tri par billes à voies multiples, systèmes et procédés d'utilisation de ceux-ci au moyen de sources de pression |
| WO2023083999A2 (fr) | 2021-11-10 | 2023-05-19 | Dna Script | Nouvelles variantes de la désoxynucléotidyl transférase terminale (tdt) |
| WO2023083997A2 (fr) | 2021-11-10 | 2023-05-19 | Dna Script | Nouveau désoxynucléotidyl terminal |
| CN116555216A (zh) * | 2022-01-28 | 2023-08-08 | 中国科学院天津工业生物技术研究所 | 可控合成单链dna的末端转移酶变体及应用 |
| WO2023170266A1 (fr) | 2022-03-11 | 2023-09-14 | Dna Script | Station d'automatisation pour la synthèse enzymatique de polynucléotides |
| WO2023170286A2 (fr) | 2022-03-11 | 2023-09-14 | Dna Script | Montant d'alignement et mécanisme sécurisé pour synthèse enzymatique de polynucléotides |
| WO2023170258A1 (fr) | 2022-03-11 | 2023-09-14 | Dna Script | Appareil de synthèse enzymatique d'une pluralité de polynucléotides comprenant un piège à condensation |
| WO2023170259A1 (fr) | 2022-03-11 | 2023-09-14 | Dna Script | Support d'accessoire modulaire |
| WO2023240202A2 (fr) * | 2022-06-08 | 2023-12-14 | Ansa Biotechnologies, Inc. | Polymérases désoxynucléotidyl transférases terminales modifiées |
| WO2024013278A1 (fr) | 2022-07-13 | 2024-01-18 | Dna Script | Procédé de purification d'une solution comprenant des polynucléotides |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5004689A (en) | 1982-02-22 | 1991-04-02 | Biogen, Massachusetts | DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields |
| IL71691A (en) | 1984-04-27 | 1991-04-15 | Yeda Res & Dev | Production of interferon-ypsilon |
| US5047335A (en) | 1988-12-21 | 1991-09-10 | The Regents Of The University Of Calif. | Process for controlling intracellular glycosylation of proteins |
| BG52073B2 (en) | 1990-01-24 | 1996-04-30 | Inst Molekuljarna Biolog | Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine |
| US6812339B1 (en) * | 2000-09-08 | 2004-11-02 | Applera Corporation | Polymorphisms in known genes associated with human disease, methods of detection and uses thereof |
| CA2742593C (fr) * | 2008-11-03 | 2020-05-05 | Bjarne Faurholm | Adn polymerases chimeriques |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
| EP3699283A1 (fr) * | 2014-10-20 | 2020-08-26 | Molecular Assemblies Inc. | Enzymes indépendantes de la matrice modifiées pour la synthèse de polydésoxynucléotides |
-
2016
- 2016-06-14 FR FR1655475A patent/FR3052462A1/fr active Pending
-
2017
- 2017-06-13 CA CA3024184A patent/CA3024184A1/fr active Pending
- 2017-06-13 KR KR1020187034921A patent/KR102522263B1/ko active IP Right Grant
- 2017-06-13 SG SG10202009688QA patent/SG10202009688QA/en unknown
- 2017-06-13 JP JP2018564828A patent/JP7118510B2/ja active Active
- 2017-06-13 SG SG11201809961TA patent/SG11201809961TA/en unknown
- 2017-06-13 AU AU2017286477A patent/AU2017286477C1/en active Active
- 2017-06-13 WO PCT/FR2017/051519 patent/WO2017216472A2/fr unknown
- 2017-06-13 CN CN201780037427.0A patent/CN109477080A/zh active Pending
- 2017-06-13 EP EP17746156.3A patent/EP3469075A2/fr active Pending
- 2017-06-13 US US16/309,414 patent/US20200002690A1/en not_active Abandoned
- 2017-07-07 FR FR1756408A patent/FR3053699B1/fr active Active
-
2018
- 2018-12-04 IL IL263503A patent/IL263503A/en unknown
-
2022
- 2022-06-30 JP JP2022105854A patent/JP2022137127A/ja active Pending
- 2022-07-27 US US17/815,490 patent/US20230167421A1/en active Pending
-
2023
- 2023-11-21 US US18/516,616 patent/US20240124855A1/en active Pending
- 2023-12-13 AU AU2023282219A patent/AU2023282219A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IL263503A (en) | 2019-02-03 |
| JP2022137127A (ja) | 2022-09-21 |
| EP3469075A2 (fr) | 2019-04-17 |
| CA3024184A1 (fr) | 2017-12-21 |
| FR3052462A1 (fr) | 2017-12-15 |
| CN109477080A (zh) | 2019-03-15 |
| JP2019517799A (ja) | 2019-06-27 |
| US20230167421A1 (en) | 2023-06-01 |
| US20200002690A1 (en) | 2020-01-02 |
| AU2017286477C1 (en) | 2023-12-21 |
| US20240124855A1 (en) | 2024-04-18 |
| KR102522263B1 (ko) | 2023-04-18 |
| AU2017286477A1 (en) | 2018-12-06 |
| JP7118510B2 (ja) | 2022-08-16 |
| FR3053699B1 (fr) | 2021-04-02 |
| KR20190024883A (ko) | 2019-03-08 |
| FR3053699A1 (fr) | 2018-01-12 |
| AU2017286477B2 (en) | 2023-09-14 |
| WO2017216472A2 (fr) | 2017-12-21 |
| AU2023282219A1 (en) | 2024-01-18 |
| SG10202009688QA (en) | 2020-11-27 |
| WO2017216472A3 (fr) | 2018-03-15 |
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