SG11201609053YA - Multiplexed single cell gene expression analysis using template switch and tagmentation - Google Patents
Multiplexed single cell gene expression analysis using template switch and tagmentationInfo
- Publication number
- SG11201609053YA SG11201609053YA SG11201609053YA SG11201609053YA SG11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA
- Authority
- SG
- Singapore
- Prior art keywords
- tagmentation
- gene expression
- single cell
- expression analysis
- cell gene
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985983P | 2014-04-29 | 2014-04-29 | |
| US201461987433P | 2014-05-01 | 2014-05-01 | |
| PCT/US2015/028062 WO2015168161A2 (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201609053YA true SG11201609053YA (en) | 2016-11-29 |
Family
ID=53055134
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201912516SA SG10201912516SA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| SG11201609053YA SG11201609053YA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201912516SA SG10201912516SA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20170044525A1 (de) |
| EP (2) | EP3680333A1 (de) |
| JP (1) | JP6412954B2 (de) |
| CN (1) | CN106459967A (de) |
| AU (4) | AU2015253299B2 (de) |
| CA (2) | CA3060708A1 (de) |
| SG (2) | SG10201912516SA (de) |
| WO (1) | WO2015168161A2 (de) |
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2015
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- 2015-04-28 CA CA3060708A patent/CA3060708A1/en active Pending
- 2015-04-28 SG SG10201912516SA patent/SG10201912516SA/en unknown
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| EP3137601B1 (de) | 2020-04-08 |
| JP2017514487A (ja) | 2017-06-08 |
| JP6412954B2 (ja) | 2018-10-24 |
| AU2021203246B2 (en) | 2023-11-30 |
| US20170044525A1 (en) | 2017-02-16 |
| WO2015168161A2 (en) | 2015-11-05 |
| AU2015253299A1 (en) | 2016-11-17 |
| EP3137601A2 (de) | 2017-03-08 |
| AU2024201323A1 (en) | 2024-03-21 |
| AU2018226493B2 (en) | 2021-02-25 |
| EP3680333A1 (de) | 2020-07-15 |
| WO2015168161A3 (en) | 2015-12-30 |
| CN106459967A (zh) | 2017-02-22 |
| US20210079386A1 (en) | 2021-03-18 |
| AU2018226493A1 (en) | 2018-09-27 |
| CA2947426C (en) | 2020-01-07 |
| AU2015253299B2 (en) | 2018-06-14 |
| SG10201912516SA (en) | 2020-02-27 |
| CA2947426A1 (en) | 2015-11-05 |
| CA3060708A1 (en) | 2015-11-05 |
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