SG11201608585RA - Modified transposases for improved insertion sequence bias and increased dna input tolerance - Google Patents

Modified transposases for improved insertion sequence bias and increased dna input tolerance

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Publication number
SG11201608585RA
SG11201608585RA SG11201608585RA SG11201608585RA SG11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA
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Singapore
Prior art keywords
insertion sequence
increased dna
dna input
improved insertion
sequence bias
Prior art date
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SG11201608585RA
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English (en)
Inventor
Christian Gloeckner
Amirali Kia
Erin Bomati
Molly He
Haiying Li Grunenwald
Scott Kuersten
Trina Faye Osothprarop
Darin Haskins
Joshua Burgess
Anupama Khanna
Daniel Schlingman
Ramesh Vaidyanathan
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Illumina Inc
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Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of SG11201608585RA publication Critical patent/SG11201608585RA/en

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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1068Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201608585RA 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance SG11201608585RA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461979871P 2014-04-15 2014-04-15
US201462062006P 2014-10-09 2014-10-09
US201462080882P 2014-11-17 2014-11-17
PCT/US2015/025889 WO2015160895A2 (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance

Publications (1)

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SG11201608585RA true SG11201608585RA (en) 2016-11-29

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US (4) US9790476B2 (https=)
EP (2) EP3845640A3 (https=)
JP (1) JP6490710B2 (https=)
CN (3) CN120098956A (https=)
AU (3) AU2015247779B2 (https=)
CA (2) CA3262557A1 (https=)
SG (1) SG11201608585RA (https=)
TW (1) TWI682997B (https=)
WO (1) WO2015160895A2 (https=)

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