SG11201603045VA - Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells - Google Patents

Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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Publication number
SG11201603045VA
SG11201603045VA SG11201603045VA SG11201603045VA SG11201603045VA SG 11201603045V A SG11201603045V A SG 11201603045VA SG 11201603045V A SG11201603045V A SG 11201603045VA SG 11201603045V A SG11201603045V A SG 11201603045VA SG 11201603045V A SG11201603045V A SG 11201603045VA
Authority
SG
Singapore
Prior art keywords
clustering
differentiation
suspension
pluripotent stem
cells
Prior art date
Application number
SG11201603045VA
Other languages
English (en)
Inventor
Benjamin Fryer
Daina Laniauskas
Marcia Blackmoore
Haiyun Wang
Kostadinka Lilova
Shelley Nelson
Elizabeth Rosocha
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of SG11201603045VA publication Critical patent/SG11201603045VA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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SG11201603045VA 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells SG11201603045VA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
PCT/US2014/038993 WO2015065524A2 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Publications (1)

Publication Number Publication Date
SG11201603045VA true SG11201603045VA (en) 2016-05-30

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SG11201603045VA SG11201603045VA (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
SG11201603047PA SG11201603047PA (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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Country Status (12)

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EP (2) EP3063268A4 (ko)
JP (2) JP2016534731A (ko)
KR (4) KR20180128529A (ko)
CN (2) CN105793413A (ko)
AU (4) AU2014342995C1 (ko)
BR (1) BR112016009393A8 (ko)
CA (2) CA2928639A1 (ko)
MX (2) MX2016005657A (ko)
PH (2) PH12016500783A1 (ko)
RU (2) RU2689710C2 (ko)
SG (2) SG11201603045VA (ko)
WO (2) WO2015065524A2 (ko)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201603045VA (en) * 2013-11-01 2016-05-30 Janssen Biotech Inc Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
MX2018016055A (es) * 2016-06-23 2019-07-04 Tithon Biotech Inc Células que expresan receptor de hormona paratiroidea 1 y usos de las mismas.
KR102516844B1 (ko) * 2016-11-16 2023-04-04 시나타 세라퓨틱스 엘티디 만능성 줄기 세포 분석
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
WO2019018818A1 (en) * 2017-07-21 2019-01-24 Semma Therapeutics, Inc. REAGGREGATION OF BETA PANCREATIC CELLS DERIVED FROM STEM CELLS
JP7000896B2 (ja) * 2017-08-23 2022-01-19 王子ホールディングス株式会社 細胞シート形成部材、細胞シート形成部材の製造方法、および、細胞シートの製造方法
EP3735457A4 (en) * 2018-01-05 2021-08-25 Platelet Biogenesis, Inc. COMPOSITIONS AND METHOD OF MANUFACTURING MEGAKARYOCYTE
CN112041428B (zh) * 2018-01-18 2024-05-24 新加坡科技研究局 用于在悬浮培养物中分化人多能干细胞系的方法
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법
US10724052B2 (en) 2018-09-07 2020-07-28 Crispr Therapeutics Ag Universal donor cells
AU2020284245B2 (en) 2019-05-31 2023-10-05 Viacyte, Inc. A biocompatible membrane composite
CN114206480B (zh) 2019-05-31 2024-06-04 W.L.戈尔及同仁股份有限公司 生物相容性膜复合材料
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
US20220233299A1 (en) 2019-05-31 2022-07-28 W. L. Gore & Associates, Inc. Cell encapsulation devices with controlled oxygen diffusion distances
US11118195B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
JP2022547053A (ja) 2019-09-05 2022-11-10 クリスパー セラピューティクス アクチェンゲゼルシャフト ユニバーサルドナー細胞
CN114729323A (zh) * 2019-11-22 2022-07-08 诺和诺德股份有限公司 旋转聚集的神经微球及其应用
EP4271796A1 (en) 2020-12-31 2023-11-08 CRISPR Therapeutics AG Universal donor cells
JPWO2022203051A1 (ko) * 2021-03-25 2022-09-29

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUP0500699A3 (en) * 2001-11-09 2010-01-28 Artecel Sciences Endocrine pancreas differentiation of adipose tissue-derived stromal cells and uses thereof
DK3441459T3 (da) * 2006-08-02 2021-06-07 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur
JP5067949B2 (ja) * 2006-11-09 2012-11-07 独立行政法人国立国際医療研究センター 霊長類動物胚性幹細胞の培養及び継代方法、並びにその分化誘導方法
US20090053182A1 (en) * 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
KR101732952B1 (ko) * 2007-07-01 2017-05-08 라이프스캔, 인코포레이티드 단일 다분화성 줄기 세포 배양
GB0800524D0 (en) * 2008-01-14 2008-02-20 Univ Brighton Cell culture system
JP2011514169A (ja) * 2008-03-17 2011-05-06 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 幹細胞培養のためのマイクロキャリア
KR101829310B1 (ko) * 2008-06-30 2018-02-14 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
ES2590036T3 (es) * 2009-08-12 2016-11-17 Kyoto University Método para inducir la diferenciación de células madre pluripotentes en células precursoras neurales
US8932857B2 (en) * 2010-06-15 2015-01-13 Kyoto University Method for selecting reduced differentiation resistance human induced pluripotent stem cells
US9085757B2 (en) * 2010-06-17 2015-07-21 Regents Of The University Of Minnesota Production of insulin producing cells
BR112013004514A2 (pt) * 2010-08-31 2016-06-07 Janssen Biotech Inc diferenciação das células-tronco embrionárias humanas
MY177150A (en) * 2011-02-28 2020-09-08 Stempeutics Res Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2013056072A1 (en) * 2011-10-13 2013-04-18 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
RU2705001C2 (ru) * 2011-12-22 2019-11-01 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в одногормональные инсулинположительные клетки
US10519422B2 (en) * 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
CA2896750A1 (en) * 2012-12-31 2014-07-03 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
SG11201603045VA (en) * 2013-11-01 2016-05-30 Janssen Biotech Inc Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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