SG11201505618XA - Process for detection of dna modifications and protein binding by single molecule manipulation - Google Patents
Process for detection of dna modifications and protein binding by single molecule manipulationInfo
- Publication number
- SG11201505618XA SG11201505618XA SG11201505618XA SG11201505618XA SG11201505618XA SG 11201505618X A SG11201505618X A SG 11201505618XA SG 11201505618X A SG11201505618X A SG 11201505618XA SG 11201505618X A SG11201505618X A SG 11201505618XA SG 11201505618X A SG11201505618X A SG 11201505618XA
- Authority
- SG
- Singapore
- Prior art keywords
- detection
- protein binding
- single molecule
- dna modifications
- molecule manipulation
- Prior art date
Links
- 230000008836 DNA modification Effects 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/557—Immunoassay; Biospecific binding assay; Materials therefor using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13305074 | 2013-01-22 | ||
| PCT/EP2014/051272 WO2014114687A1 (fr) | 2013-01-22 | 2014-01-22 | Procédé de détection de modifications d'adn et de liaison de protéine par une seule manipulation moléculaire |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201505618XA true SG11201505618XA (en) | 2015-08-28 |
Family
ID=47683668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201505618XA SG11201505618XA (en) | 2013-01-22 | 2014-01-22 | Process for detection of dna modifications and protein binding by single molecule manipulation |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US9915655B2 (fr) |
| EP (1) | EP2948774B1 (fr) |
| JP (1) | JP6461008B2 (fr) |
| KR (1) | KR102022427B1 (fr) |
| CN (2) | CN108588208B (fr) |
| AU (1) | AU2014209942B2 (fr) |
| BR (1) | BR112015017354A2 (fr) |
| CA (1) | CA2898151C (fr) |
| DK (1) | DK2948774T3 (fr) |
| ES (1) | ES2689676T3 (fr) |
| HK (1) | HK1215602A1 (fr) |
| IL (1) | IL239926B (fr) |
| LT (1) | LT2948774T (fr) |
| RU (1) | RU2678996C2 (fr) |
| SG (1) | SG11201505618XA (fr) |
| WO (1) | WO2014114687A1 (fr) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010037001A2 (fr) | 2008-09-26 | 2010-04-01 | Immune Disease Institute, Inc. | Oxydation sélective de 5-méthylcytosine par des protéines de la famille tet |
| ES2872073T3 (es) | 2011-12-13 | 2021-11-02 | Univ Oslo Hf | Procedimientos y kits de detección de estado de metilación |
| ES2669512T3 (es) | 2012-11-30 | 2018-05-28 | Cambridge Epigenetix Limited | Agente oxidante para nucleótidos modificados |
| US20170298412A1 (en) * | 2014-09-29 | 2017-10-19 | Fujirebio Inc | Method and kit for measuring target nucleic acid containing modified nucleobase |
| EP3090803B1 (fr) | 2015-05-07 | 2019-08-07 | Paris Sciences et Lettres - Quartier Latin | Dispositif amélioré pour l'analyse de molécules d'acide nucléique |
| US10196683B2 (en) | 2015-05-07 | 2019-02-05 | Paris Sciences Et Lettres—Quartier Latin | Formation of hairpins in situ using force-induced strand invasion |
| US11459573B2 (en) | 2015-09-30 | 2022-10-04 | Trustees Of Boston University | Deadman and passcode microbial kill switches |
| KR101928659B1 (ko) | 2017-06-19 | 2018-12-13 | 서울대학교병원 | Dna 변형 유도 단백질 복합체 및 이를 암호화하는 유전자를 포함하는 재조합 벡터 |
| WO2019030306A1 (fr) | 2017-08-08 | 2019-02-14 | Depixus | Isolement et enrichissement in vitro d'acides nucléiques à l'aide de nucléases spécifiques à un site |
| CN110470844A (zh) * | 2018-05-09 | 2019-11-19 | 中国科学院物理研究所 | 基于解旋酶检测dna与蛋白质相互作用的方法 |
| CA3120176A1 (fr) | 2018-11-16 | 2020-05-22 | Depixus | Optimisation d'isolement in vitro d'acides nucleiques a l'aide de nucleases a site specifique |
| EP3837379B1 (fr) | 2018-12-12 | 2022-05-18 | Depixus | Procédé d'enrichissement d'acide nucléique à l'aide de nucléases spécifiques à un site suivi de capture hybride |
| CN110396535B (zh) * | 2019-05-08 | 2022-12-23 | 南开大学 | 单分子技术检测cgi序列甲基化调控cxxc结构域位点特异性结合的方法 |
| CN111269911B (zh) * | 2020-01-23 | 2023-05-02 | 南开大学 | 包括CpG位点的发夹结构及测量CpG毗邻序列影响蛋白解离时间常数的单分子力学方法 |
| AU2021319150A1 (en) | 2020-07-30 | 2023-03-02 | Cambridge Epigenetix Limited | Compositions and methods for nucleic acid analysis |
| EP4160199A1 (fr) | 2021-10-04 | 2023-04-05 | Depixus | Appareil d'analyse de biomolécules à l'aide d'un puits et d'une cavité au-dessous du puits |
| WO2023187759A1 (fr) * | 2022-04-01 | 2023-10-05 | Depixus SAS | Procédés de criblage de composés |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0329198B1 (fr) | 1981-04-17 | 1998-06-10 | Yale University | Oligonucléotides et polynucléotides modifiés à bases et méthodes pour la preparation et l'utilisation des mêmes |
| IL73577A (en) | 1983-12-12 | 1989-10-31 | Miles Lab | Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid |
| CA1223222A (fr) | 1984-02-22 | 1987-06-23 | Nanibhushan Dattagupta | Sonde moleculaire contenant un acide nucleique immobilise |
| DE69206080T2 (de) | 1991-03-21 | 1996-06-13 | Johnson & Johnson Clin Diag | Element und verfahren für nuklein-säure amplifikation und nachweis unter verwendung von adhärierten sonden. |
| US7052650B2 (en) | 1997-03-28 | 2006-05-30 | Center National De La Recherche Scientifique (Cnrs) | Apparatus and method for the manipulation and testing of molecules, and in particular of DNA |
| US7244391B2 (en) | 1997-03-28 | 2007-07-17 | Centre National De La Recherche Scientifique (Cnrs) | Apparatus and method for the manipulation and testing of molecules, and in particular of DNA |
| US6238869B1 (en) * | 1997-12-19 | 2001-05-29 | High Throughput Genomics, Inc. | High throughput assay system |
| JP2004500020A (ja) * | 1999-03-30 | 2004-01-08 | ナノゲン・インコーポレイテッド | 半導体マイクロチップ上の電子的ドットブロットアッセイによる一塩基多型の識別 |
| US9249464B2 (en) * | 2004-11-29 | 2016-02-02 | Sequenom, Inc. | Kits and methods for detecting methylated DNA |
| CN100424174C (zh) * | 2005-09-01 | 2008-10-08 | 上海交通大学 | 原子力显微镜诱导单分子dna定位突变方法 |
| EP2053379A1 (fr) * | 2007-10-24 | 2009-04-29 | Nambition GmbH | Procédé et appareil pour l'analyse des traces de force |
| EP2390351A1 (fr) * | 2010-05-27 | 2011-11-30 | Centre National de la Recherche Scientifique (CNRS) | Procédé de séquençage de l'ADN par hybridisation |
| EP2390350A1 (fr) * | 2010-05-27 | 2011-11-30 | Centre National de la Recherche Scientifique (CNRS) | Procédé de séquençage de l'ADN par polymérisation |
-
2014
- 2014-01-22 BR BR112015017354A patent/BR112015017354A2/pt not_active IP Right Cessation
- 2014-01-22 LT LTEP14701960.8T patent/LT2948774T/lt unknown
- 2014-01-22 KR KR1020157022687A patent/KR102022427B1/ko active IP Right Grant
- 2014-01-22 US US14/761,944 patent/US9915655B2/en active Active
- 2014-01-22 CN CN201810287691.6A patent/CN108588208B/zh active Active
- 2014-01-22 JP JP2015553132A patent/JP6461008B2/ja active Active
- 2014-01-22 DK DK14701960.8T patent/DK2948774T3/en active
- 2014-01-22 EP EP14701960.8A patent/EP2948774B1/fr active Active
- 2014-01-22 CN CN201480017389.9A patent/CN105122063A/zh active Pending
- 2014-01-22 SG SG11201505618XA patent/SG11201505618XA/en unknown
- 2014-01-22 WO PCT/EP2014/051272 patent/WO2014114687A1/fr active Application Filing
- 2014-01-22 AU AU2014209942A patent/AU2014209942B2/en active Active
- 2014-01-22 RU RU2015132798A patent/RU2678996C2/ru active
- 2014-01-22 ES ES14701960.8T patent/ES2689676T3/es active Active
- 2014-01-22 CA CA2898151A patent/CA2898151C/fr active Active
-
2015
- 2015-07-14 IL IL239926A patent/IL239926B/en active IP Right Grant
-
2016
- 2016-03-24 HK HK16103518.9A patent/HK1215602A1/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP6461008B2 (ja) | 2019-01-30 |
| DK2948774T3 (en) | 2018-09-24 |
| EP2948774B1 (fr) | 2018-07-04 |
| US20150362488A1 (en) | 2015-12-17 |
| JP2016507744A (ja) | 2016-03-10 |
| AU2014209942B2 (en) | 2019-01-31 |
| KR102022427B1 (ko) | 2019-09-18 |
| CN105122063A (zh) | 2015-12-02 |
| CN108588208B (zh) | 2022-05-24 |
| CA2898151C (fr) | 2021-05-25 |
| HK1215602A1 (zh) | 2016-09-02 |
| EP2948774A1 (fr) | 2015-12-02 |
| AU2014209942A1 (en) | 2015-08-27 |
| ES2689676T3 (es) | 2018-11-15 |
| US9915655B2 (en) | 2018-03-13 |
| CN108588208A (zh) | 2018-09-28 |
| IL239926A0 (en) | 2015-08-31 |
| RU2015132798A (ru) | 2017-03-02 |
| WO2014114687A1 (fr) | 2014-07-31 |
| KR20160003629A (ko) | 2016-01-11 |
| RU2678996C2 (ru) | 2019-02-05 |
| CA2898151A1 (fr) | 2014-07-31 |
| IL239926B (en) | 2018-08-30 |
| LT2948774T (lt) | 2018-10-10 |
| BR112015017354A2 (pt) | 2017-11-21 |
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