SG11201502138WA - USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS - Google Patents
USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDSInfo
- Publication number
- SG11201502138WA SG11201502138WA SG11201502138WA SG11201502138WA SG11201502138WA SG 11201502138W A SG11201502138W A SG 11201502138WA SG 11201502138W A SG11201502138W A SG 11201502138WA SG 11201502138W A SG11201502138W A SG 11201502138WA SG 11201502138W A SG11201502138W A SG 11201502138WA
- Authority
- SG
- Singapore
- Prior art keywords
- proteases
- detergents
- extraction
- low
- nucleic acids
- Prior art date
Links
- 102000035195 Peptidases Human genes 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 title 1
- 239000004365 Protease Substances 0.000 title 1
- 239000003599 detergent Substances 0.000 title 1
- 238000000605 extraction Methods 0.000 title 1
- 150000002500 ions Chemical class 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261703112P | 2012-09-19 | 2012-09-19 | |
| PCT/US2013/060349 WO2014047141A1 (en) | 2012-09-19 | 2013-09-18 | USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201502138WA true SG11201502138WA (en) | 2015-05-28 |
Family
ID=49304332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201502138WA SG11201502138WA (en) | 2012-09-19 | 2013-09-18 | USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS |
Country Status (10)
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2843202T3 (es) | 2012-09-28 | 2021-07-16 | Cepheid | Métodos para la extracción de ADN y ARN a partir de muestras de tejido fijado embebido en parafina |
| CN104749009B (zh) * | 2015-03-30 | 2018-05-04 | 上海云泽生物科技有限公司 | 用于免疫分析的免疫抑制剂药物提取试剂 |
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| KR101927720B1 (ko) * | 2017-01-04 | 2018-12-11 | 국민대학교산학협력단 | 필터 추출용 조성물 및 이를 이용한 식품 내 미생물 분리 방법 |
| CN108531472B (zh) * | 2017-03-05 | 2021-07-16 | 北京新羿生物科技有限公司 | 一种用于核酸提取的裂解液 |
| EP3631006A4 (en) | 2017-06-01 | 2020-06-03 | Nantomics, LLC | DNA STABILIZATION OF RNA |
| CN107254463B (zh) * | 2017-06-20 | 2018-11-20 | 迈克生物股份有限公司 | 用于提取丙型肝炎病毒rna的试剂盒 |
| CN109306349A (zh) * | 2017-07-26 | 2019-02-05 | 天津迦美惠众科技有限公司 | 一种基于纳米磁珠的生物样品核酸提取纯化方法 |
| CN107794260A (zh) * | 2017-11-21 | 2018-03-13 | 苏州吉玛基因股份有限公司 | 一种从大体积无细胞体液中提取游离核酸的方法 |
| SG11202104814XA (en) * | 2019-01-25 | 2021-08-30 | Sekisui Chemical Co Ltd | Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip |
| CN111154753A (zh) * | 2020-03-09 | 2020-05-15 | 重庆市大足区人民医院 | 一种磁珠法细菌核酸提取液 |
| CN115667510A (zh) * | 2020-05-25 | 2023-01-31 | 积水医疗株式会社 | 核酸的纯化方法 |
| EP4215610A4 (en) * | 2020-09-15 | 2024-10-16 | Sekisui Medical Co., Ltd. | NUCLEIC ACID PURIFICATION PROCESS, NUCLEIC ACID EXTRACTION FLUID AND NUCLEIC ACID PURIFICATION KIT |
| KR20220075978A (ko) * | 2020-11-30 | 2022-06-08 | (주)바이오니아 | 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법 |
| JP2025506366A (ja) * | 2022-02-05 | 2025-03-11 | ベクトン・ディキンソン・アンド・カンパニー | 非不透明溶解緩衝液組成物製剤 |
| CN114410747A (zh) * | 2022-03-30 | 2022-04-29 | 北京中海生物科技有限公司 | 一种病毒免提试剂及其在病毒基因组扩增中的应用 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1301606C (en) * | 1986-05-02 | 1992-05-26 | David H. Gillespie | Chaotropic method for evaluating nucleic acids in a biological sample |
| JP3856174B2 (ja) * | 1997-12-11 | 2006-12-13 | 東洋紡績株式会社 | 植物ｄｎａの抽出精製方法 |
| US6664379B1 (en) * | 1999-09-24 | 2003-12-16 | Ambion, Inc. | Nuclease inhibitor cocktail |
| US20030203384A1 (en) * | 2002-03-08 | 2003-10-30 | Chafin David R. | Multiplex detection of biological materials in a sample |
| US7998699B2 (en) * | 2002-08-15 | 2011-08-16 | University Of South Florida | Early detection of pathogens in blood |
| EP1559783A1 (de) * | 2004-01-29 | 2005-08-03 | Qiagen GmbH | Verfahren zur chromatographischen Trennung eines Nukleinsäuregemisches |
| WO2006052680A1 (en) * | 2004-11-05 | 2006-05-18 | Qiagen North American Holdings, Inc. | Compositions and methods for purifying nucleic acids from stabilization reagents |
| CN101400689A (zh) * | 2006-02-08 | 2009-04-01 | 鲁米金公司 | 提取rna的方法 |
| US20070185322A1 (en) * | 2006-02-08 | 2007-08-09 | Nexgen Diagnostics Llc | Methods of extracting RNA |
| US20070190526A1 (en) * | 2006-02-16 | 2007-08-16 | Nexgen Diagnostics Llc | Methods of extracting nucleic acids |
| EP1911844A1 (en) * | 2006-10-10 | 2008-04-16 | Qiagen GmbH | Methods and kit for isolating nucleic acids |
| WO2009094597A2 (en) * | 2008-01-24 | 2009-07-30 | Bioventures, Inc. | Use of nucleic acid probes to detect nucleotide sequences of interest in a sample |
| JP5641191B2 (ja) * | 2010-02-26 | 2014-12-17 | Jsr株式会社 | ウイルスの検出方法 |
| US20110111410A1 (en) * | 2009-11-09 | 2011-05-12 | Streck, Inc. | Stabilization of rna in intact cells within a blood sample |
| RS56636B1 (sr) * | 2010-01-07 | 2018-03-30 | Bigtec Private Ltd | Postupak i komplet za izolaciju nukleinskih kiselina |
| US8420801B2 (en) * | 2010-01-08 | 2013-04-16 | Roche Molecular Systems, Inc. | Recovery of nucleic acids from magnetic glass particles |
| EP2598654B2 (en) * | 2010-07-29 | 2023-08-02 | F. Hoffmann-La Roche AG | Generic sample preparation |
-
2013
- 2013-09-18 IN IN2479DEN2015 patent/IN2015DN02479A/en unknown
- 2013-09-18 US US14/030,195 patent/US20140087366A1/en not_active Abandoned
- 2013-09-18 AU AU2013318156A patent/AU2013318156A1/en not_active Abandoned
- 2013-09-18 BR BR112015005718A patent/BR112015005718A2/pt not_active IP Right Cessation
- 2013-09-18 SG SG11201502138WA patent/SG11201502138WA/en unknown
- 2013-09-18 WO PCT/US2013/060349 patent/WO2014047141A1/en unknown
- 2013-09-18 EP EP13773470.3A patent/EP2898069B1/en active Active
- 2013-09-18 KR KR1020157006945A patent/KR20150054844A/ko not_active Withdrawn
- 2013-09-18 CN CN201380047926.XA patent/CN105008534A/zh active Pending
- 2013-09-18 JP JP2015532169A patent/JP2015528313A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| BR112015005718A2 (pt) | 2017-07-04 |
| EP2898069B1 (en) | 2018-04-11 |
| AU2013318156A1 (en) | 2015-03-19 |
| IN2015DN02479A (enrdf_load_stackoverflow) | 2015-09-11 |
| JP2015528313A (ja) | 2015-09-28 |
| CN105008534A (zh) | 2015-10-28 |
| EP2898069A1 (en) | 2015-07-29 |
| WO2014047141A1 (en) | 2014-03-27 |
| US20140087366A1 (en) | 2014-03-27 |
| KR20150054844A (ko) | 2015-05-20 |
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