SE7711853L - STABILIZED LIQUID COMPOSITIONS OF ENZYMES AND COENZYMES AND THEIR PREPARATION - Google Patents

STABILIZED LIQUID COMPOSITIONS OF ENZYMES AND COENZYMES AND THEIR PREPARATION

Info

Publication number
SE7711853L
SE7711853L SE7711853A SE7711853A SE7711853L SE 7711853 L SE7711853 L SE 7711853L SE 7711853 A SE7711853 A SE 7711853A SE 7711853 A SE7711853 A SE 7711853A SE 7711853 L SE7711853 L SE 7711853L
Authority
SE
Sweden
Prior art keywords
enzymes
coenzymes
stabilised
preparation
liquid compositions
Prior art date
Application number
SE7711853A
Other languages
Swedish (sv)
Other versions
SE446742B (en
Original Assignee
Modrovich Ivan Endre
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Modrovich Ivan Endre filed Critical Modrovich Ivan Endre
Publication of SE7711853L publication Critical patent/SE7711853L/en
Publication of SE446742B publication Critical patent/SE446742B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
    • C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
    • C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D307/62—Three oxygen atoms, e.g. ascorbic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Labile coenzymes such as nicotinamide adenine dinucleotide (NAD) and/or enzymes are stabilised by treatment with an organic solvent in aqueous medium while the pH is adjusted within a range of 6.0 to 8.5. An azide may be added to the solution, not only as bacteriostatic agent, but also as stabilising agent. A second coenzyme, such as adenosine triphosphate, may also be stabilised in the same solution. One or more enzymes, such as hexokinase or glucose-6-phosphate dehydrogenase, may also be stabilised against denaturation in the same solution. The process yields a very stable composition which can be used in assays for biological diagnosis.
SE7711853A 1976-09-13 1977-10-20 STABILIZED FLUID Aqueous Mixture CONTAINING ENZYMES AND / OR COENZYMS FOR USE BY BIOLOGICAL AND DIAGNOSTIC DETERMINATIONS AND SETTING TO STABILIZE Aqueous Enzyme and / or Coenzyme SE446742B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US72256576A 1976-09-13 1976-09-13

Publications (2)

Publication Number Publication Date
SE7711853L true SE7711853L (en) 1979-04-21
SE446742B SE446742B (en) 1986-10-06

Family

ID=24902389

Family Applications (1)

Application Number Title Priority Date Filing Date
SE7711853A SE446742B (en) 1976-09-13 1977-10-20 STABILIZED FLUID Aqueous Mixture CONTAINING ENZYMES AND / OR COENZYMS FOR USE BY BIOLOGICAL AND DIAGNOSTIC DETERMINATIONS AND SETTING TO STABILIZE Aqueous Enzyme and / or Coenzyme

Country Status (7)

Country Link
JP (2) JPS5843078B2 (en)
CA (1) CA1102225A (en)
CH (1) CH631208A5 (en)
DE (1) DE2740957A1 (en)
FR (1) FR2364457A1 (en)
GB (1) GB1570971A (en)
SE (1) SE446742B (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1102225A (en) * 1976-09-13 1981-06-02 Ivan E. Modrovich Stabilized liquid enzyme and coenzyme compositions and method of preparing same
DE2814154A1 (en) * 1978-04-01 1979-10-11 Behringwerke Ag STABILIZED NICOTINAMIDE NUCLEOTIDES AND METHOD FOR MANUFACTURING THEM
JPS5513008A (en) * 1978-07-11 1980-01-29 Mitsui Toatsu Chem Inc Stabilization of aqueous solution of enzyme
CA1187388A (en) * 1978-09-20 1985-05-21 American Monitor Corporation Stabilization of working reagent solutions containing nadh, nadph, and/or enzymes, and the use of such stabilized reagents in enzymes or substrate assays
JPS55138399A (en) * 1979-04-13 1980-10-29 Dainippon Pharmaceut Co Ltd Composition for determining beta-d-galactosidase and method thereof
JPS5982398A (en) * 1982-11-01 1984-05-12 Toyobo Co Ltd Method for stabilizing coenzyme
JPS6095746U (en) * 1983-12-08 1985-06-29 株式会社ニツコー Radio control toy radio transmitter
JPS62101773A (en) * 1985-10-29 1987-05-12 白木金属工業株式会社 Electronic key apparatus
US5298406A (en) * 1992-09-14 1994-03-29 E. I. Du Pont De Nemours And Company Formulation for stabilizing enzymatic activity and immunoreactivity of creatine kinase and creatine kinase isoenzymes
DE10313972A1 (en) * 2003-03-27 2004-10-21 Degussa Ag Coupled cofactor-dependent enzymatic reaction system
JP4986281B2 (en) * 2004-10-05 2012-07-25 旭化成ファーマ株式会社 Coenzyme stabilization method and composition thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1084080A (en) * 1964-11-30 Beckman Instruments Inc
DE1673194A1 (en) * 1965-07-22 1970-10-29 Dresden Arzneimittel Method and means for the determination of transaminase activities
US3296094A (en) * 1966-05-05 1967-01-03 Baxter Laboratories Inc Stabilized aqueous enzyme solutions
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation
US3627688A (en) * 1968-11-12 1971-12-14 Procter & Gamble Stabilized aqueous enzyme containing compositions
GB1322951A (en) * 1969-10-29 1973-07-11 Warner Lambert Co Process and agent for the determination of glucose
DE2061984C3 (en) * 1970-12-16 1974-04-11 Boehringer Mannheim Gmbh, 6800 Mannheim Reagent for the determination of lactate dehydrogenase in the color test
DE2302721C2 (en) * 1973-01-19 1975-01-23 Boehringer Mannheim Gmbh, 6800 Mannheim Method for the determination of creatine kinase
US3920798A (en) * 1973-05-08 1975-11-18 Union Carbide Corp Zeolite y synthesis
US3962037A (en) * 1975-01-30 1976-06-08 Miles Laboratories, Inc. Composition for stabilizing an enzyme-containing reagent
NL7603588A (en) * 1975-04-21 1976-10-25 Hoffmann La Roche STABILIZED COENZYME SOLUTION.
JPS52111183A (en) * 1976-03-12 1977-09-17 Hitachi Ltd Hand rail guiding device
CA1091174A (en) * 1976-03-17 1980-12-09 Ivan E. Modrovich Stabilized liquid enzyme
FR2344570A1 (en) * 1976-03-17 1977-10-14 Modrovich Ivan PROCESS FOR STABILIZING A LABILE COENZYME
CA1102225A (en) * 1976-09-13 1981-06-02 Ivan E. Modrovich Stabilized liquid enzyme and coenzyme compositions and method of preparing same

Also Published As

Publication number Publication date
JPS5843078B2 (en) 1983-09-24
CA1102225A (en) 1981-06-02
SE446742B (en) 1986-10-06
JPS5352685A (en) 1978-05-13
GB1570971A (en) 1980-07-09
JPH0147999B2 (en) 1989-10-17
JPS60105494A (en) 1985-06-10
FR2364457A1 (en) 1978-04-07
FR2364457B1 (en) 1980-04-25
DE2740957A1 (en) 1978-03-16
CH631208A5 (en) 1982-07-30
DE2740957C2 (en) 1987-07-30

Similar Documents

Publication Publication Date Title
Barrio et al. A fluorescent analog of nicotinamide adenine dinucleotide
Dale et al. The synthesis and enzymatic polymerization of nucleotides containing mercury: potential tools for nucleic acid sequencing and structural analysis
Secrist III et al. Fluorescent modification of adenosine-containing coenzymes. Biological activities and spectroscopic properties
Strominger et al. Uridine diphosphoglucose dehydrogenase of pea seedlings
Judah et al. The inhibition of oxidative phosphorylation
SE7711853L (en) STABILIZED LIQUID COMPOSITIONS OF ENZYMES AND COENZYMES AND THEIR PREPARATION
DK57388A (en) THIAZOLIDINO DERIVATIVES, PROCEDURE FOR THEIR PREPARATION AND THE USE OF IT
FI901916A0 (en) DIAGNOSTIC TESTFOERPACKNING, PRIMERKOMPOSITION OCH DERAS ANVAENDNING VID REPLIKERING ELLER DETEKTERING AV NUKLEINSYROR.
US20050214759A1 (en) Enzymatic redox labelling of nucleic acids
EP0276375A3 (en) Process for the preparation of optically active alpha-hydroxynitriles
US4242505A (en) S-Adenosyl-L-methionine compositions and production thereof
GB1417538A (en) Process and reagent for the determination of blood ammonia
MINATO et al. Studies on Nonspecific Adenosine Deaminase from Takadiastase I. Purification and Properties
GB1491560A (en) Reagent composition and method for determination of glycerol concentrations
ES382365A1 (en) Method for preparing cytidine diphosphate choline
DK671688D0 (en) 2- (PIPERAZINYL) -2-OXOETHYLENE SUBSTITUTED FLAVONOID DERIVATIVES, PROCEDURES FOR PREPARING IT, AND PHARMACEUTICALS CONTAINING THESE
KR830005848A (en) Method for Preparation of Stabilized Freeze-Dried Formulation of β-lactam Compound
Datta et al. Oxidation of 2, 5-diketogluconate by a cell-free enzyme preparation from Acetobacter melanogenum
Jastorff et al. Synthesis and biological activities of cyclic AMP analogs modified in the 1, 2, and 2′-positions
DE102006035020B4 (en) Stabilization of NAD / NADH
EP0331745A4 (en) Ferrocene derivatives, surfactants containing same, and process for producing thin organic film
Barrett Nucleoside triphosphate metabolism in the muscle tissue of Ascaris lumbricoides (Nematoda)
DK348489A (en) PROCEDURE FOR THE PREPARATION OF (S) -CYAN HYDRAINES
JPS54103844A (en) Method of stabilizing formulation of prostaglandin and its analogs
KR830005221A (en) Synthesis method of diazaprine nucleoside

Legal Events

Date Code Title Description
NUG Patent has lapsed

Ref document number: 7711853-7

Effective date: 19870915

Format of ref document f/p: F