SE7711853L - STABILIZED LIQUID COMPOSITIONS OF ENZYMES AND COENZYMES AND THEIR PREPARATION - Google Patents
STABILIZED LIQUID COMPOSITIONS OF ENZYMES AND COENZYMES AND THEIR PREPARATIONInfo
- Publication number
- SE7711853L SE7711853L SE7711853A SE7711853A SE7711853L SE 7711853 L SE7711853 L SE 7711853L SE 7711853 A SE7711853 A SE 7711853A SE 7711853 A SE7711853 A SE 7711853A SE 7711853 L SE7711853 L SE 7711853L
- Authority
- SE
- Sweden
- Prior art keywords
- enzymes
- coenzymes
- stabilised
- preparation
- liquid compositions
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
- C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D307/62—Three oxygen atoms, e.g. ascorbic acid
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Emergency Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Labile coenzymes such as nicotinamide adenine dinucleotide (NAD) and/or enzymes are stabilised by treatment with an organic solvent in aqueous medium while the pH is adjusted within a range of 6.0 to 8.5. An azide may be added to the solution, not only as bacteriostatic agent, but also as stabilising agent. A second coenzyme, such as adenosine triphosphate, may also be stabilised in the same solution. One or more enzymes, such as hexokinase or glucose-6-phosphate dehydrogenase, may also be stabilised against denaturation in the same solution. The process yields a very stable composition which can be used in assays for biological diagnosis.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US72256576A | 1976-09-13 | 1976-09-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
SE7711853L true SE7711853L (en) | 1979-04-21 |
SE446742B SE446742B (en) | 1986-10-06 |
Family
ID=24902389
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE7711853A SE446742B (en) | 1976-09-13 | 1977-10-20 | STABILIZED FLUID Aqueous Mixture CONTAINING ENZYMES AND / OR COENZYMS FOR USE BY BIOLOGICAL AND DIAGNOSTIC DETERMINATIONS AND SETTING TO STABILIZE Aqueous Enzyme and / or Coenzyme |
Country Status (7)
Country | Link |
---|---|
JP (2) | JPS5843078B2 (en) |
CA (1) | CA1102225A (en) |
CH (1) | CH631208A5 (en) |
DE (1) | DE2740957A1 (en) |
FR (1) | FR2364457A1 (en) |
GB (1) | GB1570971A (en) |
SE (1) | SE446742B (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1102225A (en) * | 1976-09-13 | 1981-06-02 | Ivan E. Modrovich | Stabilized liquid enzyme and coenzyme compositions and method of preparing same |
DE2814154A1 (en) * | 1978-04-01 | 1979-10-11 | Behringwerke Ag | STABILIZED NICOTINAMIDE NUCLEOTIDES AND METHOD FOR MANUFACTURING THEM |
JPS5513008A (en) * | 1978-07-11 | 1980-01-29 | Mitsui Toatsu Chem Inc | Stabilization of aqueous solution of enzyme |
CA1187388A (en) * | 1978-09-20 | 1985-05-21 | American Monitor Corporation | Stabilization of working reagent solutions containing nadh, nadph, and/or enzymes, and the use of such stabilized reagents in enzymes or substrate assays |
JPS55138399A (en) * | 1979-04-13 | 1980-10-29 | Dainippon Pharmaceut Co Ltd | Composition for determining beta-d-galactosidase and method thereof |
JPS5982398A (en) * | 1982-11-01 | 1984-05-12 | Toyobo Co Ltd | Method for stabilizing coenzyme |
JPS6095746U (en) * | 1983-12-08 | 1985-06-29 | 株式会社ニツコー | Radio control toy radio transmitter |
JPS62101773A (en) * | 1985-10-29 | 1987-05-12 | 白木金属工業株式会社 | Electronic key apparatus |
US5298406A (en) * | 1992-09-14 | 1994-03-29 | E. I. Du Pont De Nemours And Company | Formulation for stabilizing enzymatic activity and immunoreactivity of creatine kinase and creatine kinase isoenzymes |
DE10313972A1 (en) * | 2003-03-27 | 2004-10-21 | Degussa Ag | Coupled cofactor-dependent enzymatic reaction system |
JP4986281B2 (en) * | 2004-10-05 | 2012-07-25 | 旭化成ファーマ株式会社 | Coenzyme stabilization method and composition thereof |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1084080A (en) * | 1964-11-30 | Beckman Instruments Inc | ||
DE1673194A1 (en) * | 1965-07-22 | 1970-10-29 | Dresden Arzneimittel | Method and means for the determination of transaminase activities |
US3296094A (en) * | 1966-05-05 | 1967-01-03 | Baxter Laboratories Inc | Stabilized aqueous enzyme solutions |
US3557002A (en) * | 1967-11-15 | 1971-01-19 | Procter & Gamble | Stabilized aqueous enzyme preparation |
US3627688A (en) * | 1968-11-12 | 1971-12-14 | Procter & Gamble | Stabilized aqueous enzyme containing compositions |
GB1322951A (en) * | 1969-10-29 | 1973-07-11 | Warner Lambert Co | Process and agent for the determination of glucose |
DE2061984C3 (en) * | 1970-12-16 | 1974-04-11 | Boehringer Mannheim Gmbh, 6800 Mannheim | Reagent for the determination of lactate dehydrogenase in the color test |
DE2302721C2 (en) * | 1973-01-19 | 1975-01-23 | Boehringer Mannheim Gmbh, 6800 Mannheim | Method for the determination of creatine kinase |
US3920798A (en) * | 1973-05-08 | 1975-11-18 | Union Carbide Corp | Zeolite y synthesis |
US3962037A (en) * | 1975-01-30 | 1976-06-08 | Miles Laboratories, Inc. | Composition for stabilizing an enzyme-containing reagent |
NL7603588A (en) * | 1975-04-21 | 1976-10-25 | Hoffmann La Roche | STABILIZED COENZYME SOLUTION. |
JPS52111183A (en) * | 1976-03-12 | 1977-09-17 | Hitachi Ltd | Hand rail guiding device |
CA1091174A (en) * | 1976-03-17 | 1980-12-09 | Ivan E. Modrovich | Stabilized liquid enzyme |
FR2344570A1 (en) * | 1976-03-17 | 1977-10-14 | Modrovich Ivan | PROCESS FOR STABILIZING A LABILE COENZYME |
CA1102225A (en) * | 1976-09-13 | 1981-06-02 | Ivan E. Modrovich | Stabilized liquid enzyme and coenzyme compositions and method of preparing same |
-
1977
- 1977-08-31 CA CA285,845A patent/CA1102225A/en not_active Expired
- 1977-09-08 GB GB3744577A patent/GB1570971A/en not_active Expired
- 1977-09-09 FR FR7727386A patent/FR2364457A1/en active Granted
- 1977-09-12 DE DE19772740957 patent/DE2740957A1/en active Granted
- 1977-09-12 CH CH1111877A patent/CH631208A5/en not_active IP Right Cessation
- 1977-09-13 JP JP52111183A patent/JPS5843078B2/en not_active Expired
- 1977-10-20 SE SE7711853A patent/SE446742B/en not_active IP Right Cessation
-
1984
- 1984-09-29 JP JP20545084A patent/JPS60105494A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS5843078B2 (en) | 1983-09-24 |
CA1102225A (en) | 1981-06-02 |
SE446742B (en) | 1986-10-06 |
JPS5352685A (en) | 1978-05-13 |
GB1570971A (en) | 1980-07-09 |
JPH0147999B2 (en) | 1989-10-17 |
JPS60105494A (en) | 1985-06-10 |
FR2364457A1 (en) | 1978-04-07 |
FR2364457B1 (en) | 1980-04-25 |
DE2740957A1 (en) | 1978-03-16 |
CH631208A5 (en) | 1982-07-30 |
DE2740957C2 (en) | 1987-07-30 |
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Legal Events
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NUG | Patent has lapsed |
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