JPS55138399A - Composition for determining beta-d-galactosidase and method thereof - Google Patents
Composition for determining beta-d-galactosidase and method thereofInfo
- Publication number
- JPS55138399A JPS55138399A JP4591279A JP4591279A JPS55138399A JP S55138399 A JPS55138399 A JP S55138399A JP 4591279 A JP4591279 A JP 4591279A JP 4591279 A JP4591279 A JP 4591279A JP S55138399 A JPS55138399 A JP S55138399A
- Authority
- JP
- Japan
- Prior art keywords
- galactosidase
- composition
- nitrophenyl
- alcohol
- determined
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: A composition that is composed of o-nitrophenyl-β-galactopyroside and a specific alcohol, thus permitting very high-sensitivity determination of the activity of β-galactosidase.
CONSTITUTION: o-Nitrophenyl-β-D-galactopyranoside is dissolved in 5W100, preferably 20W80vol% of at least one alcohol selected from ethylene glycol, methanol, propylene glycol, n-propanol, glycerol, and triethylene glycol. The solution is added to a sample to be determined so that the alcohol concentration becomes 0.5W20% and the yellow color of the nitrophenol liberated by the action of β-D-galactosidase is determined by colorimetry.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4591279A JPS55138399A (en) | 1979-04-13 | 1979-04-13 | Composition for determining beta-d-galactosidase and method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4591279A JPS55138399A (en) | 1979-04-13 | 1979-04-13 | Composition for determining beta-d-galactosidase and method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55138399A true JPS55138399A (en) | 1980-10-29 |
JPS6342520B2 JPS6342520B2 (en) | 1988-08-24 |
Family
ID=12732449
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4591279A Granted JPS55138399A (en) | 1979-04-13 | 1979-04-13 | Composition for determining beta-d-galactosidase and method thereof |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55138399A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52134087A (en) * | 1976-03-17 | 1977-11-09 | Modrovich Ivan Endre | Stabilized liquid coenzyme composition |
JPS5352685A (en) * | 1976-09-13 | 1978-05-13 | Modrovich Ivan Endre | Stabilized liquid enzyme and coenzyme composition and production thereof |
-
1979
- 1979-04-13 JP JP4591279A patent/JPS55138399A/en active Granted
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52134087A (en) * | 1976-03-17 | 1977-11-09 | Modrovich Ivan Endre | Stabilized liquid coenzyme composition |
JPS5352685A (en) * | 1976-09-13 | 1978-05-13 | Modrovich Ivan Endre | Stabilized liquid enzyme and coenzyme composition and production thereof |
Also Published As
Publication number | Publication date |
---|---|
JPS6342520B2 (en) | 1988-08-24 |
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