FR2364457A1 - STABILIZATION PROCESS OF A CO-ENZYME AND OF A LABILE ENZYME - Google Patents

STABILIZATION PROCESS OF A CO-ENZYME AND OF A LABILE ENZYME

Info

Publication number
FR2364457A1
FR2364457A1 FR7727386A FR7727386A FR2364457A1 FR 2364457 A1 FR2364457 A1 FR 2364457A1 FR 7727386 A FR7727386 A FR 7727386A FR 7727386 A FR7727386 A FR 7727386A FR 2364457 A1 FR2364457 A1 FR 2364457A1
Authority
FR
France
Prior art keywords
enzyme
labile
stabilized
enzymes
stabilization process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7727386A
Other languages
French (fr)
Other versions
FR2364457B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MODROVICH IVAN
Original Assignee
MODROVICH IVAN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MODROVICH IVAN filed Critical MODROVICH IVAN
Publication of FR2364457A1 publication Critical patent/FR2364457A1/en
Application granted granted Critical
Publication of FR2364457B1 publication Critical patent/FR2364457B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
    • C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
    • C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D307/62—Three oxygen atoms, e.g. ascorbic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un procédé de stabilisation d'un coenzyme et d'un enzyme labiles. Des co-enzymes labiles tels que le nicotinamide-adénine-dinucléotide (NAD) sont stabilisés par traitement d'un solvant organique en milieu aqueux dans un polymère convenable n'inhibant pas l'activité enzymatique. Un azoture peut être ajouté à la solution, non seulement comme agent bactériostatique, mais aussi comme agent stabilisant. Un second enzyme tel que le triphosphate d'adénosine peut aussi être stabilisé dans la même solution. Un ou plusieurs enzymes tels que l'hexokinase ou la glucose-6-phosphate-déshydrogénase peuvent également être stabilisés contre une dénaturation dans la même solution. Le procédé donne une composition très stable qui peut être utilisée dans des dosages pour le diagnostic biologique.The invention relates to a method of stabilizing a labile coenzyme and an enzyme. Labile co-enzymes such as nicotinamide-adenine-dinucleotide (NAD) are stabilized by treatment of an organic solvent in aqueous medium in a suitable polymer which does not inhibit the enzymatic activity. Azide can be added to the solution, not only as a bacteriostatic agent, but also as a stabilizing agent. A second enzyme such as ATP can also be stabilized in the same solution. One or more enzymes such as hexokinase or glucose-6-phosphate-dehydrogenase can also be stabilized against denaturation in the same solution. The method gives a very stable composition which can be used in assays for biological diagnosis.

FR7727386A 1976-09-13 1977-09-09 STABILIZATION PROCESS OF A CO-ENZYME AND OF A LABILE ENZYME Granted FR2364457A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US72256576A 1976-09-13 1976-09-13

Publications (2)

Publication Number Publication Date
FR2364457A1 true FR2364457A1 (en) 1978-04-07
FR2364457B1 FR2364457B1 (en) 1980-04-25

Family

ID=24902389

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7727386A Granted FR2364457A1 (en) 1976-09-13 1977-09-09 STABILIZATION PROCESS OF A CO-ENZYME AND OF A LABILE ENZYME

Country Status (7)

Country Link
JP (2) JPS5843078B2 (en)
CA (1) CA1102225A (en)
CH (1) CH631208A5 (en)
DE (1) DE2740957A1 (en)
FR (1) FR2364457A1 (en)
GB (1) GB1570971A (en)
SE (1) SE446742B (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1102225A (en) * 1976-09-13 1981-06-02 Ivan E. Modrovich Stabilized liquid enzyme and coenzyme compositions and method of preparing same
DE2814154A1 (en) * 1978-04-01 1979-10-11 Behringwerke Ag STABILIZED NICOTINAMIDE NUCLEOTIDES AND METHOD FOR MANUFACTURING THEM
JPS5513008A (en) * 1978-07-11 1980-01-29 Mitsui Toatsu Chem Inc Stabilization of aqueous solution of enzyme
CA1187388A (en) * 1978-09-20 1985-05-21 American Monitor Corporation Stabilization of working reagent solutions containing nadh, nadph, and/or enzymes, and the use of such stabilized reagents in enzymes or substrate assays
JPS55138399A (en) * 1979-04-13 1980-10-29 Dainippon Pharmaceut Co Ltd Composition for determining beta-d-galactosidase and method thereof
JPS5982398A (en) * 1982-11-01 1984-05-12 Toyobo Co Ltd Method for stabilizing coenzyme
JPS6095746U (en) * 1983-12-08 1985-06-29 株式会社ニツコー Radio control toy radio transmitter
JPS62101773A (en) * 1985-10-29 1987-05-12 白木金属工業株式会社 Electronic key apparatus
US5298406A (en) * 1992-09-14 1994-03-29 E. I. Du Pont De Nemours And Company Formulation for stabilizing enzymatic activity and immunoreactivity of creatine kinase and creatine kinase isoenzymes
DE10313972A1 (en) * 2003-03-27 2004-10-21 Degussa Ag Coupled cofactor-dependent enzymatic reaction system
WO2006038647A1 (en) * 2004-10-05 2006-04-13 Asahi Kasei Pharma Corporation Method for stabilizing coenzyme and composition therefor

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2308639A1 (en) * 1975-04-21 1976-11-19 Hoffmann La Roche STABLE SOLUTION OF A COENZYME
FR2344568A1 (en) * 1976-03-17 1977-10-14 Modrovich Ivan PROCESS FOR STABILIZING A LABILE ENZYME
FR2344570A1 (en) * 1976-03-17 1977-10-14 Modrovich Ivan PROCESS FOR STABILIZING A LABILE COENZYME

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1084079A (en) * 1964-11-30 Beckman Instruments Inc
DE1673194A1 (en) * 1965-07-22 1970-10-29 Dresden Arzneimittel Method and means for the determination of transaminase activities
US3296094A (en) * 1966-05-05 1967-01-03 Baxter Laboratories Inc Stabilized aqueous enzyme solutions
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation
US3627688A (en) * 1968-11-12 1971-12-14 Procter & Gamble Stabilized aqueous enzyme containing compositions
GB1322951A (en) * 1969-10-29 1973-07-11 Warner Lambert Co Process and agent for the determination of glucose
DE2061984C3 (en) * 1970-12-16 1974-04-11 Boehringer Mannheim Gmbh, 6800 Mannheim Reagent for the determination of lactate dehydrogenase in the color test
DE2302721C2 (en) * 1973-01-19 1975-01-23 Boehringer Mannheim Gmbh, 6800 Mannheim Method for the determination of creatine kinase
US3920798A (en) * 1973-05-08 1975-11-18 Union Carbide Corp Zeolite y synthesis
US3962037A (en) * 1975-01-30 1976-06-08 Miles Laboratories, Inc. Composition for stabilizing an enzyme-containing reagent
JPS52111183A (en) * 1976-03-12 1977-09-17 Hitachi Ltd Hand rail guiding device
CA1102225A (en) * 1976-09-13 1981-06-02 Ivan E. Modrovich Stabilized liquid enzyme and coenzyme compositions and method of preparing same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2308639A1 (en) * 1975-04-21 1976-11-19 Hoffmann La Roche STABLE SOLUTION OF A COENZYME
FR2344568A1 (en) * 1976-03-17 1977-10-14 Modrovich Ivan PROCESS FOR STABILIZING A LABILE ENZYME
FR2344570A1 (en) * 1976-03-17 1977-10-14 Modrovich Ivan PROCESS FOR STABILIZING A LABILE COENZYME

Also Published As

Publication number Publication date
GB1570971A (en) 1980-07-09
DE2740957A1 (en) 1978-03-16
CA1102225A (en) 1981-06-02
JPS5843078B2 (en) 1983-09-24
SE7711853L (en) 1979-04-21
JPS5352685A (en) 1978-05-13
FR2364457B1 (en) 1980-04-25
JPH0147999B2 (en) 1989-10-17
CH631208A5 (en) 1982-07-30
JPS60105494A (en) 1985-06-10
DE2740957C2 (en) 1987-07-30
SE446742B (en) 1986-10-06

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