SE456164B - Forfarande for immobilisering, odling och efterfoljande frigoring av animala celler samt mikroberare av gelatin med absorberade animala celler - Google Patents
Forfarande for immobilisering, odling och efterfoljande frigoring av animala celler samt mikroberare av gelatin med absorberade animala cellerInfo
- Publication number
- SE456164B SE456164B SE8005838A SE8005838A SE456164B SE 456164 B SE456164 B SE 456164B SE 8005838 A SE8005838 A SE 8005838A SE 8005838 A SE8005838 A SE 8005838A SE 456164 B SE456164 B SE 456164B
- Authority
- SE
- Sweden
- Prior art keywords
- cells
- microcarrier
- gelatin
- animal cells
- alginate
- Prior art date
Links
- 108010010803 Gelatin Proteins 0.000 title claims abstract description 20
- 229920000159 gelatin Polymers 0.000 title claims abstract description 20
- 239000008273 gelatin Substances 0.000 title claims abstract description 20
- 235000019322 gelatine Nutrition 0.000 title claims abstract description 20
- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 10
- 210000004027 cell Anatomy 0.000 claims abstract description 42
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000001419 dependent effect Effects 0.000 claims abstract description 6
- 239000002245 particle Substances 0.000 claims abstract description 5
- 230000006378 damage Effects 0.000 claims abstract description 4
- 239000003431 cross linking reagent Substances 0.000 claims abstract 5
- 239000000696 magnetic material Substances 0.000 claims abstract 5
- 210000004102 animal cell Anatomy 0.000 claims abstract 4
- 230000003100 immobilizing effect Effects 0.000 claims description 3
- 230000007515 enzymatic degradation Effects 0.000 claims 2
- 108010007093 dispase Proteins 0.000 abstract description 7
- 102000029816 Collagenase Human genes 0.000 abstract description 5
- 108060005980 Collagenase Proteins 0.000 abstract description 5
- 229960002424 collagenase Drugs 0.000 abstract description 5
- 229920000936 Agarose Polymers 0.000 abstract description 4
- 235000010443 alginic acid Nutrition 0.000 abstract description 4
- 229920000615 alginic acid Polymers 0.000 abstract description 4
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 abstract description 4
- 238000011282 treatment Methods 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 4
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 abstract 3
- 229940072056 alginate Drugs 0.000 abstract 3
- 102000004877 Insulin Human genes 0.000 abstract 2
- 108090001061 Insulin Proteins 0.000 abstract 2
- 229940125396 insulin Drugs 0.000 abstract 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N CHCl3 Substances ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 abstract 1
- 229920001661 Chitosan Polymers 0.000 abstract 1
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 abstract 1
- 230000032823 cell division Effects 0.000 abstract 1
- 150000004676 glycans Chemical class 0.000 abstract 1
- 210000004153 islets of langerhan Anatomy 0.000 abstract 1
- 229920001282 polysaccharide Polymers 0.000 abstract 1
- 239000005017 polysaccharide Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 239000004094 surface-active agent Substances 0.000 abstract 1
- 239000011324 bead Substances 0.000 description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 102000004142 Trypsin Human genes 0.000 description 5
- 108090000631 Trypsin Proteins 0.000 description 5
- 230000010261 cell growth Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 239000012588 trypsin Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 4
- 239000002953 phosphate buffered saline Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000004677 Nylon Substances 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- CUNWUEBNSZSNRX-RKGWDQTMSA-N (2r,3r,4r,5s)-hexane-1,2,3,4,5,6-hexol;(z)-octadec-9-enoic acid Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.CCCCCCCC\C=C/CCCCCCCC(O)=O.CCCCCCCC\C=C/CCCCCCCC(O)=O.CCCCCCCC\C=C/CCCCCCCC(O)=O CUNWUEBNSZSNRX-RKGWDQTMSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 239000012979 RPMI medium Substances 0.000 description 1
- 206010038389 Renal cancer Diseases 0.000 description 1
- 208000006265 Renal cell carcinoma Diseases 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- LQEJKDNALLXRCT-UHFFFAOYSA-N chloroform;toluene Chemical compound ClC(Cl)Cl.CC1=CC=CC=C1 LQEJKDNALLXRCT-UHFFFAOYSA-N 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 201000010174 renal carcinoma Diseases 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000001626 skin fibroblast Anatomy 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B60—VEHICLES IN GENERAL
- B60P—VEHICLES ADAPTED FOR LOAD TRANSPORTATION OR TO TRANSPORT, TO CARRY, OR TO COMPRISE SPECIAL LOADS OR OBJECTS
- B60P1/00—Vehicles predominantly for transporting loads and modified to facilitate loading, consolidating the load, or unloading
- B60P1/44—Vehicles predominantly for transporting loads and modified to facilitate loading, consolidating the load, or unloading having a loading platform thereon raising the load to the level of the load-transporting element
- B60P1/4414—Vehicles predominantly for transporting loads and modified to facilitate loading, consolidating the load, or unloading having a loading platform thereon raising the load to the level of the load-transporting element and keeping the loading platform parallel to the ground when raising the load
- B60P1/4421—Vehicles predominantly for transporting loads and modified to facilitate loading, consolidating the load, or unloading having a loading platform thereon raising the load to the level of the load-transporting element and keeping the loading platform parallel to the ground when raising the load the loading platform being carried in at least one vertical guide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0012—Cell encapsulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
- C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/10—Mineral substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/72—Chitin, chitosan
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Transportation (AREA)
- Mechanical Engineering (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Sustainable Development (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Loading Or Unloading Of Vehicles (AREA)
- Medicinal Preparation (AREA)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8005838A SE456164B (sv) | 1980-08-20 | 1980-08-20 | Forfarande for immobilisering, odling och efterfoljande frigoring av animala celler samt mikroberare av gelatin med absorberade animala celler |
| DE19813152230 DE3152230A1 (de) | 1980-08-20 | 1981-08-14 | Immobilization of animal cells |
| GB8210512A GB2093040B (en) | 1980-08-20 | 1981-08-14 | Immobilization of animal cells |
| EP81902357A EP0058689B1 (de) | 1980-08-20 | 1981-08-14 | Zellkulturmedium, verbessertes Verfahren zum Züchten tierischer Zellen, Verfahren zur Herstellung von Mikroträgern und Mikroträger |
| PCT/US1981/001098 WO1982000660A1 (en) | 1980-08-20 | 1981-08-14 | Immobilization of animal cells |
| JP56502848A JPS57501411A (de) | 1980-08-20 | 1981-08-14 | |
| SE8602560A SE456153B (sv) | 1980-08-20 | 1986-06-06 | Lyftgaffelanordning for en lyftanordning, som er anbringad pa ett lastflak till ett lastfordon |
| SE8604739A SE456163B (sv) | 1980-08-20 | 1986-11-05 | Forfarande for att odla och skorda ytberoende animala celler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8005838A SE456164B (sv) | 1980-08-20 | 1980-08-20 | Forfarande for immobilisering, odling och efterfoljande frigoring av animala celler samt mikroberare av gelatin med absorberade animala celler |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8005838L SE8005838L (sv) | 1982-02-21 |
| SE456164B true SE456164B (sv) | 1988-09-12 |
Family
ID=20341574
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8005838A SE456164B (sv) | 1980-08-20 | 1980-08-20 | Forfarande for immobilisering, odling och efterfoljande frigoring av animala celler samt mikroberare av gelatin med absorberade animala celler |
| SE8602560A SE456153B (sv) | 1980-08-20 | 1986-06-06 | Lyftgaffelanordning for en lyftanordning, som er anbringad pa ett lastflak till ett lastfordon |
| SE8604739A SE456163B (sv) | 1980-08-20 | 1986-11-05 | Forfarande for att odla och skorda ytberoende animala celler |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8602560A SE456153B (sv) | 1980-08-20 | 1986-06-06 | Lyftgaffelanordning for en lyftanordning, som er anbringad pa ett lastflak till ett lastfordon |
| SE8604739A SE456163B (sv) | 1980-08-20 | 1986-11-05 | Forfarande for att odla och skorda ytberoende animala celler |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0058689B1 (de) |
| JP (1) | JPS57501411A (de) |
| GB (1) | GB2093040B (de) |
| SE (3) | SE456164B (de) |
| WO (1) | WO1982000660A1 (de) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA827728B (en) * | 1981-11-17 | 1983-08-31 | Nestle Sa | A process for the production of enzymatically active biocatalysts and the products obtained |
| DE3200988A1 (de) * | 1982-01-14 | 1983-07-28 | Thomas A. Dr. 6900 Heidelberg Reed | Verfahren und vorrichtung zur abtrennung von organischen stoffen aus einer suspension oder loesung |
| SE8201972L (sv) * | 1982-03-29 | 1983-09-30 | Gambro Lundia Ab | Magnetiskt paverkbara kristalliserade kolhydrat sferer eller partiklar att anvendas tillsammans med bioadsorberande material |
| EP0097907A3 (de) * | 1982-06-25 | 1985-01-09 | Flow General, Inc. | Mikroträger für Zellkultur |
| US4605623A (en) * | 1982-11-08 | 1986-08-12 | Malette William Graham | Method of altering growth and development and suppressing contamination microorganisms in cell or tissue culture |
| JPS59164723A (ja) * | 1983-03-10 | 1984-09-17 | Koken:Kk | 再生コラーゲンフイブリルを含有する基質及びその製造方法 |
| IL74259A0 (en) * | 1984-02-06 | 1985-05-31 | Surface Concepts Pty Ltd | Improved method for cell culture |
| SE8401125L (sv) * | 1984-03-01 | 1985-09-02 | Ulf Schroder | Sett vid diagnostisering in vitro och diagnostiskt medel |
| US5387522A (en) * | 1984-06-01 | 1995-02-07 | Schering Corporation | Apparatus having a biphasic spray head for entrapping biological material in a hydrophilic gel |
| AU5669486A (en) * | 1985-04-04 | 1986-10-23 | Verax Corp. | Weighted microsponge for immobilizing bioactive material |
| US5100783A (en) * | 1985-05-10 | 1992-03-31 | Verax Corporation | Weighted microsponge for immobilizing bioactive material |
| CA1265443A (en) * | 1985-09-18 | 1990-02-06 | Elieser Gorelik | Assays for chemotherapeutic agents |
| DD274336A3 (de) * | 1985-10-03 | 1989-12-20 | Npo Biolar | Verfahren zur Gewinnung einer Mikroträgersubstanz für die Zellkultivierung |
| SE464816B (sv) * | 1985-10-15 | 1991-06-17 | Nilsson Kjell | Makroporoesa partiklar, foerfarande foer dess framstaellning och dess anvaendning |
| PT83746B (pt) * | 1985-11-15 | 1988-08-17 | Gist Brocades Nv | Processo para a preparacao de novos biocatalisadores imobilizados e para a producao de etanol por fermentacao |
| JPH0634699B2 (ja) * | 1986-12-27 | 1994-05-11 | 千代田化工建設株式会社 | 動物細胞培養方法及び装置 |
| DE3735397A1 (de) * | 1987-10-20 | 1989-05-03 | Hoechst Ag | Magnetische membrankapseln und ihre verwendung |
| EP0318286A3 (de) * | 1987-11-26 | 1990-10-31 | Katakura Chikkarin Co., Ltd. | Träger für Zellkulturen, seine Herstellung und Verwendung |
| EP0417227A1 (de) * | 1989-03-15 | 1991-03-20 | KINDERVATER, Ralf | Biokatalytisches verfahren sowie trägerteilchen, das aus magnetischem glas oder keramikteilchen besteht, und vorrichtung zur durchführung |
| DK210989D0 (da) * | 1989-05-01 | 1989-05-01 | Danochemo As | Microcarriers fremstillet ved sprayteknik |
| WO1999021963A1 (de) * | 1997-10-25 | 1999-05-06 | Roche Diagnostics Gmbh | Autodegradable microcarriers and their use |
| KR19990014353A (ko) * | 1998-10-17 | 1999-02-25 | 윤태욱 | 췌도의 대량 배양증식방법 |
| US7604807B2 (en) | 2000-12-01 | 2009-10-20 | Auburn University | Use of pullulan to isolate and preserve biological material |
| EP1337830A2 (de) | 2000-12-01 | 2003-08-27 | Auburn University | Benutzung von gummi acacia (gummi arabicum) zur isolierung und konservierung von biologischem material |
| AU2003245185B2 (en) | 2002-06-11 | 2008-01-10 | Celltrix Ab | Porous gelatin material, gelatin structures, methods for preparation of the same and uses thereof |
| US20040248291A1 (en) * | 2003-04-10 | 2004-12-09 | Pentax Corporation | Method for culturing cells, cell culture carriers and cell culture apparatus |
| WO2005003332A2 (en) * | 2003-06-30 | 2005-01-13 | University Of South Florida | Diamagnetically stabilised three-dimensional cell culture |
| US20090137018A1 (en) | 2003-06-30 | 2009-05-28 | University Of South Florida | Magnetic three-dimensional cell culture apparatus and method |
| CN101416059A (zh) * | 2003-07-17 | 2009-04-22 | 格洛伯塞尔解决方案公司 | 自动化细胞培养系统及方法 |
| WO2005010139A2 (en) | 2003-07-23 | 2005-02-03 | University Of South Florida | Ferromagnetic cell and tissue culture microcarriers |
| CN1321175C (zh) * | 2004-01-13 | 2007-06-13 | 中国科学院动物研究所 | 一种用于大规模培养细胞的微载体 |
| DE102009043537B4 (de) | 2009-09-30 | 2012-03-22 | Siemens Aktiengesellschaft | Verfahren und Anordnung zur Bestimmung von Zell-Vitalitäten |
| EP2640827B1 (de) * | 2010-11-16 | 2023-07-19 | Ingeneron, Inc. | Verfahren zur konservierung von zielzellen |
| WO2018182016A1 (ja) * | 2017-03-30 | 2018-10-04 | 日産化学株式会社 | ナノファイバーを用いた細胞培養 |
| CN109125786A (zh) * | 2018-08-29 | 2019-01-04 | 佛山市森昂生物科技有限公司 | 一种高强度抑菌医用敷料的制备方法 |
| CN109624816A (zh) * | 2018-10-24 | 2019-04-16 | 唐卫 | 货车分层自动装卸技术 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3639558A (en) * | 1968-02-19 | 1972-02-01 | Louis Csizmas | Immunological reagent particles having proteinaceous materials covalently bonded thereto |
| FR2053565A5 (de) * | 1969-07-09 | 1971-04-16 | Pasteur Institut | |
| US3977941A (en) * | 1971-04-20 | 1976-08-31 | Research Corporation | Protein-enzyme complex membranes |
| BE789195A (fr) * | 1971-09-24 | 1973-03-22 | Gist Brocades Nv | Compositions d'enzymes |
| US3972776A (en) * | 1973-02-26 | 1976-08-03 | Research Corporation | Preparation of protein membranes containing microbial cells |
| US4024020A (en) * | 1975-12-15 | 1977-05-17 | Monsanto Company | Method of cell culture on polyacrylonitrile surface |
| US4036693A (en) * | 1976-02-02 | 1977-07-19 | Massachusetts Institute Of Technology | Treatment of cell culture microcarries |
| FR2353562A1 (fr) * | 1976-06-04 | 1977-12-30 | Roquette Freres | Procede de fabrication de particules insolubles a activite enzymatique |
| US4167447A (en) * | 1976-07-19 | 1979-09-11 | The United States Of America As Represented By The Secretary Of Agriculture | Method for insolubilizing enzymes on chitosan |
| US4189534A (en) * | 1976-11-11 | 1980-02-19 | Massachusetts Institute Of Technology | Cell culture microcarriers |
| FR2396083A1 (fr) * | 1977-06-30 | 1979-01-26 | Merieux Inst | Procede de culture de virus |
| DK146481C (da) * | 1978-08-14 | 1984-03-26 | Novo Industri As | Fremgangsmaade til fremstilling af et immobiliseret enzymprodukt |
| US4257884A (en) * | 1979-04-17 | 1981-03-24 | Damon Corporation | Chromatography |
| US4266032A (en) * | 1979-08-10 | 1981-05-05 | Monsanto Company | Method of culturing microcarrier supported cells |
| SE445116B (sv) * | 1979-09-12 | 1986-06-02 | Pharmacia Fine Chemicals Ab | Sett att odla celler pa mikroberare med fibronektinytskikt |
| FR2470794A1 (fr) * | 1979-12-05 | 1981-06-12 | Pasteur Institut | Nouvelles microparticules, leur preparation et leurs applications en biologie, notamment a la culture de cellules diploides humaines |
-
1980
- 1980-08-20 SE SE8005838A patent/SE456164B/sv unknown
-
1981
- 1981-08-14 WO PCT/US1981/001098 patent/WO1982000660A1/en active IP Right Grant
- 1981-08-14 GB GB8210512A patent/GB2093040B/en not_active Expired
- 1981-08-14 EP EP81902357A patent/EP0058689B1/de not_active Expired
- 1981-08-14 JP JP56502848A patent/JPS57501411A/ja active Pending
-
1986
- 1986-06-06 SE SE8602560A patent/SE456153B/sv not_active IP Right Cessation
- 1986-11-05 SE SE8604739A patent/SE456163B/sv not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| SE8604739L (sv) | 1986-11-05 |
| SE456163B (sv) | 1988-09-12 |
| JPS57501411A (de) | 1982-08-12 |
| EP0058689A1 (de) | 1982-09-01 |
| SE456153B (sv) | 1988-09-12 |
| SE8604739D0 (sv) | 1986-11-05 |
| SE8602560L (sv) | 1987-12-07 |
| EP0058689A4 (de) | 1983-02-16 |
| EP0058689B1 (de) | 1986-04-23 |
| GB2093040A (en) | 1982-08-25 |
| GB2093040B (en) | 1984-09-19 |
| SE8602560D0 (sv) | 1986-06-06 |
| SE8005838L (sv) | 1982-02-21 |
| WO1982000660A1 (en) | 1982-03-04 |
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