SA518391585B1 - طريقة تحوير عابر لإنتاج فيروس عكسي - Google Patents

طريقة تحوير عابر لإنتاج فيروس عكسي Download PDF

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Publication number
SA518391585B1
SA518391585B1 SA518391585A SA518391585A SA518391585B1 SA 518391585 B1 SA518391585 B1 SA 518391585B1 SA 518391585 A SA518391585 A SA 518391585A SA 518391585 A SA518391585 A SA 518391585A SA 518391585 B1 SA518391585 B1 SA 518391585B1
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Saudi Arabia
Prior art keywords
nucleic acid
vector
retroviral
dna
acid vector
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SA518391585A
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Arabic (ar)
English (en)
Inventor
فامفا ايرينى
جوهنسون سابين
بالانت سيليست
فينك كونراد
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جلاكسوسميثكلين انتليكشوال بروبيرتى ديفيلوبمينت ليمتد
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Priority claimed from GBGB1520764.0A external-priority patent/GB201520764D0/en
Priority claimed from GBGB1609354.4A external-priority patent/GB201609354D0/en
Application filed by جلاكسوسميثكلين انتليكشوال بروبيرتى ديفيلوبمينت ليمتد filed Critical جلاكسوسميثكلين انتليكشوال بروبيرتى ديفيلوبمينت ليمتد
Publication of SA518391585B1 publication Critical patent/SA518391585B1/ar

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C12N15/895Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection using biolistic methods
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    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16041Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16051Methods of production or purification of viral material
    • C12N2740/16052Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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    • C12N2800/00Nucleic acids vectors
    • C12N2800/20Pseudochromosomes, minichrosomosomes
    • C12N2800/204Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
    • CCHEMISTRY; METALLURGY
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    • C12N2800/00Nucleic acids vectors
    • C12N2800/22Vectors comprising a coding region that has been codon optimised for expression in a respective host
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    • C12N2800/00Nucleic acids vectors
    • C12N2800/50Vectors for producing vectors

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
SA518391585A 2015-11-24 2018-05-15 طريقة تحوير عابر لإنتاج فيروس عكسي SA518391585B1 (ar)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1520764.0A GB201520764D0 (en) 2015-11-24 2015-11-24 Transient transfection method for retroviral production
GBGB1609354.4A GB201609354D0 (en) 2016-05-26 2016-05-26 Transient transfection method for retroviral production
PCT/EP2016/078334 WO2017089307A1 (en) 2015-11-24 2016-11-21 Transient transfection method for retroviral production

Publications (1)

Publication Number Publication Date
SA518391585B1 true SA518391585B1 (ar) 2020-10-26

Family

ID=57348703

Family Applications (1)

Application Number Title Priority Date Filing Date
SA518391585A SA518391585B1 (ar) 2015-11-24 2018-05-15 طريقة تحوير عابر لإنتاج فيروس عكسي

Country Status (15)

Country Link
US (2) US10450574B2 (enExample)
EP (1) EP3380605A1 (enExample)
JP (1) JP7110096B2 (enExample)
KR (1) KR102067352B1 (enExample)
CN (1) CN108291211A (enExample)
AU (1) AU2016359838B2 (enExample)
BR (1) BR112018010639A2 (enExample)
CA (1) CA3006285A1 (enExample)
DE (1) DE102016122317A1 (enExample)
FR (1) FR3044017B1 (enExample)
IL (1) IL259223B (enExample)
IT (1) IT201600117287A1 (enExample)
RU (1) RU2749717C2 (enExample)
SA (1) SA518391585B1 (enExample)
WO (1) WO2017089307A1 (enExample)

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GB201816919D0 (en) * 2018-10-17 2018-11-28 Glaxosmithkline Ip Dev Ltd Adeno-associated viral vector producer cell lines
CN110684804B (zh) * 2019-10-15 2023-04-18 上海本导基因技术有限公司 递送外源rnp的慢病毒载体及其制备方法
GB202004371D0 (en) 2020-03-26 2020-05-13 Glaxosmithkline Ip Dev Ltd CAR constructs
GB202005096D0 (en) 2020-04-07 2020-05-20 Glaxosmithkline Modified vectors for production of retrovirus
AU2021253701B2 (en) * 2020-04-10 2024-05-02 Southwest Research Institute Three-dimensional bioreactor for viral vector production
CN117377691A (zh) 2021-03-19 2024-01-09 葛兰素史密斯克莱知识产权发展有限公司 靶向密蛋白3的嵌合抗原受体和治疗癌症的方法
CN113373266B (zh) * 2021-06-22 2022-07-01 内蒙古农业大学 一种用于检测绵羊肺腺瘤病毒的荧光rpa引物、试剂盒和检测方法
WO2024218341A1 (en) 2023-04-19 2024-10-24 Glaxosmithkline Intellectual Property Development Limited Cytotoxicity targeting chimeras for car-t and car-nk cells
WO2024218345A1 (en) 2023-04-21 2024-10-24 Glaxosmithkline Intellectual Property Development Limited Cytotoxicity targeting chimeras for antibody-drug conjugates and bispecific antibodies

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Publication number Publication date
BR112018010639A2 (pt) 2019-01-22
RU2749717C2 (ru) 2021-06-16
CA3006285A1 (en) 2017-06-01
FR3044017B1 (fr) 2019-05-03
CN108291211A (zh) 2018-07-17
AU2016359838B2 (en) 2020-08-06
IL259223A (en) 2018-07-31
RU2018118964A3 (enExample) 2020-04-02
FR3044017A1 (fr) 2017-05-26
US20200063144A1 (en) 2020-02-27
DE102016122317A1 (de) 2017-05-24
US20170145427A1 (en) 2017-05-25
KR20180073624A (ko) 2018-07-02
US10450574B2 (en) 2019-10-22
WO2017089307A1 (en) 2017-06-01
EP3380605A1 (en) 2018-10-03
IL259223B (en) 2022-05-01
KR102067352B1 (ko) 2020-01-16
IT201600117287A1 (it) 2018-05-21
RU2018118964A (ru) 2019-12-26
AU2016359838A1 (en) 2018-05-24
JP2019500030A (ja) 2019-01-10
JP7110096B2 (ja) 2022-08-01

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