RU2762685C2 - Выявление аффинно-зрелых человеческих антител - Google Patents
Выявление аффинно-зрелых человеческих антител Download PDFInfo
- Publication number
- RU2762685C2 RU2762685C2 RU2017134418A RU2017134418A RU2762685C2 RU 2762685 C2 RU2762685 C2 RU 2762685C2 RU 2017134418 A RU2017134418 A RU 2017134418A RU 2017134418 A RU2017134418 A RU 2017134418A RU 2762685 C2 RU2762685 C2 RU 2762685C2
- Authority
- RU
- Russia
- Prior art keywords
- artificial sequence
- seq
- dna
- synthetic primer
- primers
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/515—Complete light chain, i.e. VL + CL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161506749P | 2011-07-12 | 2011-07-12 | |
| US61/506,749 | 2011-07-12 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014102617A Division RU2636045C2 (ru) | 2011-07-12 | 2012-07-12 | Выявление аффинно-зрелых человеческих антител |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2017134418A RU2017134418A (ru) | 2019-02-07 |
| RU2017134418A3 RU2017134418A3 (https=) | 2021-06-10 |
| RU2762685C2 true RU2762685C2 (ru) | 2021-12-22 |
Family
ID=47506915
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017134418A RU2762685C2 (ru) | 2011-07-12 | 2012-07-12 | Выявление аффинно-зрелых человеческих антител |
| RU2014102617A RU2636045C2 (ru) | 2011-07-12 | 2012-07-12 | Выявление аффинно-зрелых человеческих антител |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014102617A RU2636045C2 (ru) | 2011-07-12 | 2012-07-12 | Выявление аффинно-зрелых человеческих антител |
Country Status (11)
| Country | Link |
|---|---|
| US (6) | US9234030B2 (https=) |
| EP (2) | EP2731965A4 (https=) |
| JP (1) | JP6731702B2 (https=) |
| KR (1) | KR102139388B1 (https=) |
| CN (2) | CN107090459A (https=) |
| AU (2) | AU2012281078B2 (https=) |
| CA (1) | CA2841475C (https=) |
| IL (2) | IL230358B (https=) |
| MX (2) | MX361600B (https=) |
| RU (2) | RU2762685C2 (https=) |
| WO (1) | WO2013009967A2 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6731702B2 (ja) * | 2011-07-12 | 2020-07-29 | エックスバイオテク, インコーポレイテッドXbiotech, Inc. | 親和性成熟化ヒト抗体の同定 |
| US10119134B2 (en) | 2013-03-15 | 2018-11-06 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| GB201316644D0 (en) * | 2013-09-19 | 2013-11-06 | Kymab Ltd | Expression vector production & High-Throughput cell screening |
| WO2015182749A1 (ja) * | 2014-05-30 | 2015-12-03 | 国立大学法人富山大学 | ＴＣＲ ｃＤＮＡの増幅方法 |
| KR101753553B1 (ko) | 2014-06-03 | 2017-07-03 | 엑스바이오테크, 인크. | 스타필로코커스 아우레우스 감염의 치료 및 예방을 위한 조성물 및 방법 |
| ES2895750T3 (es) | 2014-09-15 | 2022-02-22 | Abvitro Llc | Secuenciación de alto rendimiento de colecciones de nucleótidos |
| US10975146B2 (en) | 2018-06-29 | 2021-04-13 | Cedars-Sinai Medical Center | Interleukin-1 inhibition for combination treatment of pancreatic cancer |
| US20200392578A1 (en) * | 2019-06-14 | 2020-12-17 | The Regents Of The University Of California | Methods of sequencing antibody chains from hybridomas and kits for practicing same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2370775C2 (ru) * | 2003-07-29 | 2009-10-20 | Дженентек, Инк. | Анализ нейтрализующих антител и его применение |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE284897T1 (de) * | 2000-03-10 | 2005-01-15 | Large Scale Biology Corp | Selfantigen-impfstoffe zur behandlung von b-zell lymphomen und sonstigen krebsen |
| AU2002953381A0 (en) * | 2002-12-18 | 2003-01-09 | Diatech Pty Ltd | In vivo affinity maturation scheme |
| TWI333977B (en) | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
| PE20071101A1 (es) | 2005-08-31 | 2007-12-21 | Amgen Inc | Polipeptidos y anticuerpos |
| JP5205597B2 (ja) * | 2007-06-01 | 2013-06-05 | 静岡県 | １細胞レベルでの抗体遺伝子の解析・同定方法 |
| RU2011117213A (ru) * | 2008-09-30 | 2012-11-10 | Эббот Лэборетриз (Us) | Улучшенные библиотеки антител |
| EP2398913B1 (en) | 2009-02-20 | 2016-03-30 | F. Hoffmann-La Roche AG | Method for obtaining immunoglobulin encoding nucleic acid |
| JP6731702B2 (ja) * | 2011-07-12 | 2020-07-29 | エックスバイオテク, インコーポレイテッドXbiotech, Inc. | 親和性成熟化ヒト抗体の同定 |
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2012
- 2012-07-12 JP JP2014520314A patent/JP6731702B2/ja active Active
- 2012-07-12 KR KR1020147003416A patent/KR102139388B1/ko active Active
- 2012-07-12 RU RU2017134418A patent/RU2762685C2/ru active
- 2012-07-12 RU RU2014102617A patent/RU2636045C2/ru active
- 2012-07-12 CA CA2841475A patent/CA2841475C/en active Active
- 2012-07-12 AU AU2012281078A patent/AU2012281078B2/en active Active
- 2012-07-12 EP EP12811637.3A patent/EP2731965A4/en not_active Withdrawn
- 2012-07-12 US US13/547,497 patent/US9234030B2/en active Active
- 2012-07-12 EP EP15196994.6A patent/EP3034513B1/en active Active
- 2012-07-12 MX MX2014000431A patent/MX361600B/es active IP Right Grant
- 2012-07-12 WO PCT/US2012/046431 patent/WO2013009967A2/en not_active Ceased
- 2012-07-12 CN CN201610954453.7A patent/CN107090459A/zh active Pending
- 2012-07-12 CN CN201280034673.8A patent/CN103687873B/zh active Active
-
2014
- 2014-01-07 IL IL230358A patent/IL230358B/en active IP Right Grant
- 2014-01-10 MX MX2018015450A patent/MX2018015450A/es unknown
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2015
- 2015-11-12 US US14/939,454 patent/US9453217B2/en active Active
- 2015-11-13 US US14/940,537 patent/US9394536B2/en active Active
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2016
- 2016-08-26 US US15/248,413 patent/US9670483B2/en active Active
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2017
- 2017-05-02 US US15/584,360 patent/US20170233457A1/en not_active Abandoned
- 2017-08-03 AU AU2017210585A patent/AU2017210585B2/en active Active
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2018
- 2018-11-13 IL IL262977A patent/IL262977B/en active IP Right Grant
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2019
- 2019-11-19 US US16/688,666 patent/US20200071388A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2370775C2 (ru) * | 2003-07-29 | 2009-10-20 | Дженентек, Инк. | Анализ нейтрализующих антител и его применение |
Non-Patent Citations (5)
| Title |
|---|
| JACOB GLANVILLE et al. Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire, PNAS, 2009, vol. 106, no. 48, pp.20216-20221. * |
| JACOB GLANVILLE et al. Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire, PNAS, 2009, vol. 106, no. 48, pp.20216-20221. MICHAEL J. CAMPBELL et al. Use of family specific leader region primers for PCR amplification of the human heavy chain variable region gene repertoire, Molecular Immunology, 1992, Vol. 29, No. 2, pp. 193-203. POTJAMAS PANSERI et al. A compact phage display human scFv library for selection of antibodies to a wide variety of antigens, BMC Biotechnol. 2009, Vol.9, No.16, pp.1-16. JAMES W. LARRICK et al. Polymerase Chain Reaction Using Mixed Primers: Cloning of Human Monoclonal Antibody Variable Region Genes from Single Hybridoma Cells, Nature Biotechnology, 1989, Vol. 7, 934-993. * |
| JAMES W. LARRICK et al. Polymerase Chain Reaction Using Mixed Primers: Cloning of Human Monoclonal Antibody Variable Region Genes from Single Hybridoma Cells, Nature Biotechnology, 1989, Vol. 7, 934-993. * |
| MICHAEL J. CAMPBELL et al. Use of family specific leader region primers for PCR amplification of the human heavy chain variable region gene repertoire, Molecular Immunology, 1992, Vol. 29, No. 2, pp. 193-203. * |
| POTJAMAS PANSERI et al. A compact phage display human scFv library for selection of antibodies to a wide variety of antigens, BMC Biotechnol. 2009, Vol.9, No.16, pp.1-16. * |
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